EKS-HOS-00408

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00408

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/PDGFR	605.6	1.5E-181	600	958	359

Status

Reviewed

Ensembl Protein

ENSP00000261799

UniProt Accession

P09619; Q8N5L4;

Protein Name

Platelet-derived growth factor receptor beta

Protein Synonyms/Alias

PDGF-R-beta; PDGFR-beta; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; Platelet-derived growth factor receptor 1; PDGFR-1;

Gene Name

PDGFRB

Gene Synonyms/Alias

PDGFRB; PDGFR, PDGFR1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000113721	ENSP00000429218	ENST00000517488
ENSG00000113721	ENSP00000430026	ENST00000520579
ENSG00000113721	ENSP00000261799	ENST00000261799
ENSG00000113721	ENSP00000430715	ENST00000517957

Organism

Homo sapiens

Functional Description

Tyrosine-protein kinase that acts as cell-surfacereceptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis and migration. Plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. Required for normal development of the cardiovascular system. Required for normal recruitment of pericytes (mesangial cells) in the kidney glomerulus, and for normal formation of a branched network of capillaries in kidney glomeruli. Promotes rearrangement of the actin cytoskeleton and the formation of membrane ruffles. Binding of its cognate ligands - homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1 and NCK1. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to the activation of the AKT1 signaling pathway. Phosphorylation of SHC1, or of the C-terminus of PTPN11, creates a binding site for GRB2, resulting in the activation of HRAS, RAF1 and down-stream MAP kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation and activation of SRC family kinases. Promotes phosphorylation of PDCD6IP/ALIX and STAM. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.

Protein Length

1106

Protein Sequence
(FASTA)

MRLPGAMPAL ALKGELLLLS LLLLLEPQIS QGLVVTPPGP ELVLNVSSTF VLTCSGSAPV 60

VWERMSQEPP QEMAKAQDGT FSSVLTLTNL TGLDTGEYFC THNDSRGLET DERKRLYIFV 120

PDPTVGFLPN DAEELFIFLT EITEITIPCR VTDPQLVVTL HEKKGDVALP VPYDHQRGFS 180

GIFEDRSYIC KTTIGDREVD SDAYYVYRLQ VSSINVSVNA VQTVVRQGEN ITLMCIVIGN 240

EVVNFEWTYP RKESGRLVEP VTDFLLDMPY HIRSILHIPS AELEDSGTYT CNVTESVNDH 300

QDEKAINITV VESGYVRLLG EVGTLQFAEL HRSRTLQVVF EAYPPPTVLW FKDNRTLGDS 360

SAGEIALSTR NVSETRYVSE LTLVRVKVAE AGHYTMRAFH EDAEVQLSFQ LQINVPVRVL 420

ELSESHPDSG EQTVRCRGRG MPQPNIIWSA CRDLKRCPRE LPPTLLGNSS EEESQLETNV 480

TYWEEEQEFE VVSTLRLQHV DRPLSVRCTL RNAVGQDTQE VIVVPHSLPF KVVVISAILA 540

LVVLTIISLI ILIMLWQKKP RYEIRWKVIE SVSSDGHEYI YVDPMQLPYD STWELPRDQL 600

VLGRTLGSGA FGQVVEATAH GLSHSQATMK VAVKMLKSTA RSSEKQALMS ELKIMSHLGP 660

HLNVVNLLGA CTKGGPIYII TEYCRYGDLV DYLHRNKHTF LQHHSDKRRP PSAELYSNAL 720

PVGLPLPSHV SLTGESDGGY MDMSKDESVD YVPMLDMKGD VKYADIESSN YMAPYDNYVP 780

SAPERTCRAT LINESPVLSY MDLVGFSYQV ANGMEFLASK NCVHRDLAAR NVLICEGKLV 840

KICDFGLARD IMRDSNYISK GSTFLPLKWM APESIFNSLY TTLSDVWSFG ILLWEIFTLG 900

GTPYPELPMN EQFYNAIKRG YRMAQPAHAS DEIYEIMQKC WEEKFEIRPP FSQLVLLLER 960

LLGEGYKKKY QQVDEEFLRS DHPAILRSQA RLPGFHGLRS PLDTSSVLYT AVQPNEGDND 1020

YIIPLPDPKP EVADEGPLEG SPSLASSTLN EVNTSSTISC DSPLEPQDEP EPEPQLELQV 1080

EPEPELEQLP DSGCPAPRAE AEDSFL 1106

Nucleotide Sequence
(FASTA)

ATGCGGCTTC CGGGTGCGAT GCCAGCTCTG GCCCTCAAAG GCGAGCTGCT GTTGCTGTCT 60

CTCCTGTTAC TTCTGGAACC ACAGATCTCT CAGGGCCTGG TCGTCACACC CCCGGGGCCA 120

GAGCTTGTCC TCAATGTCTC CAGCACCTTC GTTCTGACCT GCTCGGGTTC AGCTCCGGTG 180

GTGTGGGAAC GGATGTCCCA GGAGCCCCCA CAGGAAATGG CCAAGGCCCA GGATGGCACC 240

TTCTCCAGCG TGCTCACACT GACCAACCTC ACTGGGCTAG ACACGGGAGA ATACTTTTGC 300

ACCCACAATG ACTCCCGTGG ACTGGAGACC GATGAGCGGA AACGGCTCTA CATCTTTGTG 360

CCAGATCCCA CCGTGGGCTT CCTCCCTAAT GATGCCGAGG AACTATTCAT CTTTCTCACG 420

GAAATAACTG AGATCACCAT TCCATGCCGA GTAACAGACC CACAGCTGGT GGTGACACTG 480

CACGAGAAGA AAGGGGACGT TGCACTGCCT GTCCCCTATG ATCACCAACG TGGCTTTTCT 540

GGTATCTTTG AGGACAGAAG CTACATCTGC AAAACCACCA TTGGGGACAG GGAGGTGGAT 600

TCTGATGCCT ACTATGTCTA CAGACTCCAG GTGTCATCCA TCAACGTCTC TGTGAACGCA 660

GTGCAGACTG TGGTCCGCCA GGGTGAGAAC ATCACCCTCA TGTGCATTGT GATCGGGAAT 720

GAGGTGGTCA ACTTCGAGTG GACATACCCC CGCAAAGAAA GTGGGCGGCT GGTGGAGCCG 780

GTGACTGACT TCCTCTTGGA TATGCCTTAC CACATCCGCT CCATCCTGCA CATCCCCAGT 840

GCCGAGTTAG AAGACTCGGG GACCTACACC TGCAATGTGA CGGAGAGTGT GAATGACCAT 900

CAGGATGAAA AGGCCATCAA CATCACCGTG GTTGAGAGCG GCTACGTGCG GCTCCTGGGA 960

GAGGTGGGCA CACTACAATT TGCTGAGCTG CATCGGAGCC GGACACTGCA GGTAGTGTTC 1020

GAGGCCTACC CACCGCCCAC TGTCCTGTGG TTCAAAGACA ACCGCACCCT GGGCGACTCC 1080

AGCGCTGGCG AAATCGCCCT GTCCACGCGC AACGTGTCGG AGACCCGGTA TGTGTCAGAG 1140

CTGACACTGG TTCGCGTGAA GGTGGCAGAG GCTGGCCACT ACACCATGCG GGCCTTCCAT 1200

GAGGATGCTG AGGTCCAGCT CTCCTTCCAG CTACAGATCA ATGTCCCTGT CCGAGTGCTG 1260

GAGCTAAGTG AGAGCCACCC TGACAGTGGG GAACAGACAG TCCGCTGTCG TGGCCGGGGC 1320

ATGCCCCAGC CGAACATCAT CTGGTCTGCC TGCAGAGACC TCAAAAGGTG TCCACGTGAG 1380

CTGCCGCCCA CGCTGCTGGG GAACAGTTCC GAAGAGGAGA GCCAGCTGGA GACTAACGTG 1440

ACGTACTGGG AGGAGGAGCA GGAGTTTGAG GTGGTGAGCA CACTGCGTCT GCAGCACGTG 1500

GATCGGCCAC TGTCGGTGCG CTGCACGCTG CGCAACGCTG TGGGCCAGGA CACGCAGGAG 1560

GTCATCGTGG TGCCACACTC CTTGCCCTTT AAGGTGGTGG TGATCTCAGC CATCCTGGCC 1620

CTGGTGGTGC TCACCATCAT CTCCCTTATC ATCCTCATCA TGCTTTGGCA GAAGAAGCCA 1680

CGTTACGAGA TCCGATGGAA GGTGATTGAG TCTGTGAGCT CTGACGGCCA TGAGTACATC 1740

TACGTGGACC CCATGCAGCT GCCCTATGAC TCCACGTGGG AGCTGCCGCG GGACCAGCTT 1800

GTGCTGGGAC GCACCCTCGG CTCTGGGGCC TTTGGGCAGG TGGTGGAGGC CACGGCTCAT 1860

GGCCTGAGCC ATTCTCAGGC CACGATGAAA GTGGCCGTCA AGATGCTTAA ATCCACAGCC 1920

CGCAGCAGTG AGAAGCAAGC CCTTATGTCG GAGCTGAAGA TCATGAGTCA CCTTGGGCCC 1980

CACCTGAACG TGGTCAACCT GTTGGGGGCC TGCACCAAAG GAGGACCCAT CTATATCATC 2040

ACTGAGTACT GCCGCTACGG AGACCTGGTG GACTACCTGC ACCGCAACAA ACACACCTTC 2100

CTGCAGCACC ACTCCGACAA GCGCCGCCCG CCCAGCGCGG AGCTCTACAG CAATGCTCTG 2160

CCCGTTGGGC TCCCCCTGCC CAGCCATGTG TCCTTGACCG GGGAGAGCGA CGGTGGCTAC 2220

ATGGACATGA GCAAGGACGA GTCGGTGGAC TATGTGCCCA TGCTGGACAT GAAAGGAGAC 2280

GTCAAATATG CAGACATCGA GTCCTCCAAC TACATGGCCC CTTACGATAA CTACGTTCCC 2340

TCTGCCCCTG AGAGGACCTG CCGAGCAACT TTGATCAACG AGTCTCCAGT GCTAAGCTAC 2400

ATGGACCTCG TGGGCTTCAG CTACCAGGTG GCCAATGGCA TGGAGTTTCT GGCCTCCAAG 2460

AACTGCGTCC ACAGAGACCT GGCGGCTAGG AACGTGCTCA TCTGTGAAGG CAAGCTGGTC 2520

AAGATCTGTG ACTTTGGCCT GGCTCGAGAC ATCATGCGGG ACTCGAATTA CATCTCCAAA 2580

GGCAGCACCT TTTTGCCTTT AAAGTGGATG GCTCCGGAGA GCATCTTCAA CAGCCTCTAC 2640

ACCACCCTGA GCGACGTGTG GTCCTTCGGG ATCCTGCTCT GGGAGATCTT CACCTTGGGT 2700

GGCACCCCTT ACCCAGAGCT GCCCATGAAC GAGCAGTTCT ACAATGCCAT CAAACGGGGT 2760

TACCGCATGG CCCAGCCTGC CCATGCCTCC GACGAGATCT ATGAGATCAT GCAGAAGTGC 2820

TGGGAAGAGA AGTTTGAGAT TCGGCCCCCC TTCTCCCAGC TGGTGCTGCT TCTCGAGAGA 2880

CTGTTGGGCG AAGGTTACAA AAAGAAGTAC CAGCAGGTGG ATGAGGAGTT TCTGAGGAGT 2940

GACCACCCAG CCATCCTTCG GTCCCAGGCC CGCTTGCCTG GGTTCCATGG CCTCCGATCT 3000

CCCCTGGACA CCAGCTCCGT CCTCTATACT GCCGTGCAGC CCAATGAGGG TGACAACGAC 3060

TATATCATCC CCCTGCCTGA CCCCAAACCC GAGGTTGCTG ACGAGGGCCC ACTGGAGGGT 3120

TCCCCCAGCC TAGCCAGCTC CACCCTGAAT GAAGTCAACA CCTCCTCAAC CATCTCCTGT 3180

GACAGCCCCC TGGAGCCCCA GGACGAACCA GAGCCAGAGC CCCAGCTTGA GCTCCAGGTG 3240

GAGCCGGAGC CAGAGCTGGA ACAGTTGCCG GATTCGGGGT GCCCTGCGCC TCGGGCGGAA 3300

GCAGAGGATA GCTTCCTGTA G 3321

Domain Profile

S: 1     lelGkvlGsGafGkvveatayGlsksdavlkvavkmlkstarssekealmselkilshlG  60

         l+lG++lGsGafG+vveata+Gls+s+a++kvavkmlkstarssek+almselki+shlG

Q: 600   LVLGRTLGSGAFGQVVEATAHGLSHSQATMKVAVKMLKSTARSSEKQALMSELKIMSHLG  659

         78**********************************************************

S: 61    ehenivnllGactkgGpiyviteyccyGdllnylrrkresflkkslekkkekeeeldska  120

         +h+n+vnllGactkgGpiy+iteyc+yGdl++yl+r++++fl+++++k++++++el+s+a

Q: 660   PHLNVVNLLGACTKGGPIYIITEYCRYGDLVDYLHRNKHTFLQHHSDKRRPPSAELYSNA  719

         ************************************************************

S: 121   lladkslrsksslssesseeyvdlkkd................vsdvssskadklasekk  164

         l+++  l s++sl+ es++ y+d++kd                ++d++ss++++++++++

Q: 720   LPVGLPLPSHVSLTGESDGGYMDMSKDesvdyvpmldmkgdvkYADIESSNYMAPYDNYV  779

         ************************************************************

S: 165   ksalerevkaaleeedsevleledllsfsyqvakGmeflaskncvhrdlaarnvllteGk  224

          sa er+++a+l++e s vl+++dl+ fsyqva+Gmeflaskncvhrdlaarnvl++eGk

Q: 780   PSAPERTCRATLINE-SPVLSYMDLVGFSYQVANGMEFLASKNCVHRDLAARNVLICEGK  838

         **************9.9*******************************************

S: 225   vvkicdfGlardimkdsnyvvkGnarlpvkwmapesifdslytvksdvwsyGillweifs  284

         +vkicdfGlardim+dsny++kG+++lp+kwmapesif+slyt+ sdvws+Gillweif+

Q: 839   LVKICDFGLARDIMRDSNYISKGSTFLPLKWMAPESIFNSLYTTLSDVWSFGILLWEIFT  898

         ************************************************************

S: 285   lGstpypgllvdskfyklikeGyrmaqpayateeiyeimqkcweaepekrpsfkqlvell  344

         lG+tpyp+l+++++fy++ik+Gyrmaqpa+a++eiyeimqkcwe+++e rp+f+qlv ll

Q: 899   LGGTPYPELPMNEQFYNAIKRGYRMAQPAHASDEIYEIMQKCWEEKFEIRPPFSQLVLLL  958

         ********************************************************9986

Domain Sequence
(FASTA)

LVLGRTLGSG AFGQVVEATA HGLSHSQATM KVAVKMLKST ARSSEKQALM SELKIMSHLG 60

PHLNVVNLLG ACTKGGPIYI ITEYCRYGDL VDYLHRNKHT FLQHHSDKRR PPSAELYSNA 120

LPVGLPLPSH VSLTGESDGG YMDMSKDESV DYVPMLDMKG DVKYADIESS NYMAPYDNYV 180

PSAPERTCRA TLINESPVLS YMDLVGFSYQ VANGMEFLAS KNCVHRDLAA RNVLICEGKL 240

VKICDFGLAR DIMRDSNYIS KGSTFLPLKW MAPESIFNSL YTTLSDVWSF GILLWEIFTL 300

GGTPYPELPM NEQFYNAIKR GYRMAQPAHA SDEIYEIMQK CWEEKFEIRP PFSQLVLLL 359

Keyword

3D-structure; ATP-binding; Cell membrane; Chemotaxis; Chromosomal rearrangement; Complete proteome; Cytoplasmic vesicle; Developmental protein; Direct protein sequencing; Disulfide bond; Glycoprotein; Immunoglobulin domain; Kinase; Lysosome; Membrane; Nucleotide-binding; Phosphoprotein; Polymorphism; Proto-oncogene; Receptor; Reference proteome; Repeat; Signal; Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0016324	; C:apical plasma membrane
GO:0005737	; C:cytoplasm
GO:0016023	; C:cytoplasmic membrane-bounded vesicle
GO:0005887	; C:integral to plasma membrane
GO:0031226	; C:intrinsic to plasma membrane
GO:0043202	; C:lysosomal lumen
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0004992	; F:platelet activating factor receptor activity
GO:0005019	; F:platelet-derived growth factor beta-receptor activity
GO:0048407	; F:platelet-derived growth factor binding
GO:0005021	; F:vascular endothelial growth factor-activated receptor activity
GO:0030325	; P:adrenal gland development
GO:0035909	; P:aorta morphogenesis
GO:0055003	; P:cardiac myofibril assembly
GO:0060981	; P:cell migration involved in coronary angiogenesis
GO:0035441	; P:cell migration involved in vasculogenesis
GO:0001701	; P:in utero embryonic development
GO:0072277	; P:metanephric glomerular capillary formation
GO:0072262	; P:metanephric glomerular mesangial cell proliferation involved in metanephros development
GO:0006807	; P:nitrogen compound metabolic process
GO:0018108	; P:peptidyl-tyrosine phosphorylation
GO:0046488	; P:phosphatidylinositol metabolic process
GO:0048015	; P:phosphatidylinositol-mediated signaling
GO:0090280	; P:positive regulation of calcium ion import
GO:0038091	; P:positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway
GO:0050921	; P:positive regulation of chemotaxis
GO:2000573	; P:positive regulation of DNA biosynthetic process
GO:0070374	; P:positive regulation of ERK1 and ERK2 cascade
GO:0043406	; P:positive regulation of MAP kinase activity
GO:0035793	; P:positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathway
GO:0045840	; P:positive regulation of mitosis
GO:0043552	; P:positive regulation of phosphatidylinositol 3-kinase activity
GO:0014068	; P:positive regulation of phosphatidylinositol 3-kinase cascade
GO:0010863	; P:positive regulation of phospholipase C activity
GO:0032516	; P:positive regulation of phosphoprotein phosphatase activity
GO:2000379	; P:positive regulation of reactive oxygen species metabolic process
GO:0014911	; P:positive regulation of smooth muscle cell migration
GO:0048661	; P:positive regulation of smooth muscle cell proliferation
GO:0046777	; P:protein autophosphorylation
GO:0032956	; P:regulation of actin cytoskeleton organization
GO:0050730	; P:regulation of peptidyl-tyrosine phosphorylation
GO:0061298	; P:retina vasculature development in camera-type eye
GO:0048705	; P:skeletal system morphogenesis
GO:0071670	; P:smooth muscle cell chemotaxis
GO:0048745	; P:smooth muscle tissue development
GO:0001894	; P:tissue homeostasis
GO:0048010	; P:vascular endothelial growth factor receptor signaling pathway

KEGG

hsa:5159

;

InterPros

IPR007110	; Ig-like.
IPR013783	; Ig-like_fold.
IPR013098	; Ig_I-set.
IPR003599	; Ig_sub.
IPR003598	; Ig_sub2.
IPR013151	; Immunoglobulin.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.
IPR016243	; Tyr_kinase_CSF1/PDGF_rcpt.
IPR001824	; Tyr_kinase_rcpt_3_CS.
IPR009134	; Tyr_kinase_VEGFR_rcpt_N.

Pfam

PF07679	; I-set; 1.
PF00047	; ig; 1.
PF07714	; Pkinase_Tyr; 1.

SMARTs

SM00409	; IG; 2.
SM00408	; IGc2; 1.
SM00219	; TyrKc; 1.

Prosites

PS50835	; IG_LIKE; 2.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS00240	; RECEPTOR_TYR_KIN_III; 1.

Prints

PR01832

; VEGFRECEPTOR.

Created Date

20-Feb-2013