EKS-HOS-00266

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00266

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CAMK/CAMKL	248.2	4.0E-73	783	1035	253

Status

Reviewed

Ensembl Protein

ENSP00000443083

UniProt Accession

Q96RG2; Q68DY3; Q6GSJ5; Q86XH6; Q99763; Q9UFR7;

Protein Name

PAS domain-containing serine/threonine-protein kinase

Protein Synonyms/Alias

PAS-kinase; PASKIN; hPASK;

Gene Name

PASK

Gene Synonyms/Alias

PASK; KIAA0135;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000115687	ENSP00000441374	ENST00000544142
ENSG00000115687	ENSP00000443083	ENST00000539818
ENSG00000115687	ENSP00000351475	ENST00000358649
ENSG00000115687	ENSP00000408506	ENST00000437780
ENSG00000115687	ENSP00000384438	ENST00000403638
ENSG00000115687	ENSP00000384016	ENST00000405260
ENSG00000115687	ENSP00000397922	ENST00000452907
ENSG00000115687	ENSP00000234040	ENST00000234040
ENSG00000115687	ENSP00000395672	ENST00000433589
ENSG00000115687	ENSP00000400734	ENST00000415234

Organism

Homo sapiens

Functional Description

Serine/threonine-protein kinase involved in energyhomeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells; however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate to respiratory regulation.

Protein Length

1107

Protein Sequence
(FASTA)

MKRMRQERRL CCVVVLEPVE RVSTWVAFQS DGTVTSCDSL FAHLHGYVSG EDVAGQHITD 60

LIPSVQLPPS GQHIPKNLKI QRSVGRARDG TTFPLSLKLK SQPSSEEATT GEAAPVSGYR 120

ASVWVFCTIS GLITLLPDGT IHGINHSFAL TLFGYGKTEL LGKNITFLIP GFYSYMDLAY 180

NSSLQLPDLA SCLDVGNESG CGERTLDPWQ GQDPAEGGQD PRINVVLAGG HVVPRDEIRK 240

LMESQDIFTG TQTELIAGGQ LLSCLSPQPA PGVDNVPEGS LPVHGEQALP KDQQITALGR 300

EEPVAIESPG QDLLGESRSE PVDVKPFASC EDSEAPVPAE DGGSDAGMCG LCQKAQLERM 360

GVSGPSGSDL WAGAAVAKPQ AKGQLAGGSL LMHCPCYGSE WGLWWRSQDL APSPSGMAGL 420

SFGTPTLDEP WLGVENDREE LQTCLIKEQL SQLSLAGALD VPHAELVPTE CQAVTAPVSS 480

CDLGGRDLCG GCTGSSSACY ALATDLPGGL EAVEAQEVDV NSFSWNLKEL FFSDQTDQTS 540

SNCSCATSEL RETPSSLAVG SDPDVGSLQE QGSCVLDDRE LLLLTGTCVD LGQGRRFRES 600

CVGHDPTEPL EVCLVSSEHY AASDRESPGH VPSTLDAGPE DTCPSAEEPR LNVQVTSTPV 660

IVMRGAAGLQ REIQEGAYSG SCYHRDGLRL SIQFEVRRVE LQGPTPLFCC WLVKDLLHSQ 720

RDSAARTRLF LASLPGSTHS TAAELTGPSL VEVLRARPWF EEPPKAVELE GLAACEGEYS 780

QKYSTMSPLG SGAFGFVWTA VDKEKNKEVV VKFIKKEKVL EDCWIEDPKL GKVTLEIAIL 840

SRVEHANIIK VLDIFENQGF FQLVMEKHGS GLDLFAFIDR HPRLDEPLAS YIFRQLVSAV 900

GYLRLKDIIH RDIKDENIVI AEDFTIKLID FGSAAYLERG KLFYTFCGTI EYCAPEVLMG 960

NPYRGPELEM WSLGVTLYTL VFEENPFCEL EETVEAAIHP PYLVSKELMS LVSGLLQPVP 1020

ERRTTLEKLV TDPWVTQPVN LADYTWEEVF RVNKPESGVL SAASLEMGNR SLSDVAQAQE 1080

LCGGPVPGEA PNGQGCLHPG DPRLLTS 1107

Nucleotide Sequence
(FASTA)

ATGAAGAGGA TGCGGCAGGA GCGCCGCCTA TGCTGCGTGG TGGTCCTGGA GCCCGTGGAG 60

AGGGTCTCGA CCTGGGTCGC TTTCCAGAGC GATGGCACCG TCACGTCATG TGACAGTCTC 120

TTTGCTCATC TTCACGGGTA CGTGTCTGGG GAGGACGTGG CTGGGCAGCA TATCACAGAC 180

CTGATCCCTT CTGTGCAGCT CCCTCCTTCT GGCCAGCACA TCCCAAAGAA TCTCAAGATT 240

CAGAGGTCTG TTGGAAGAGC CAGGGACGGT ACCACCTTCC CTCTGAGCTT AAAGCTGAAA 300

TCCCAACCCA GCAGCGAGGA GGCGACCACC GGTGAGGCGG CCCCTGTGAG CGGCTACCGG 360

GCATCTGTCT GGGTGTTCTG CACCATCAGT GGCCTCATCA CCCTCCTGCC GGATGGGACC 420

ATCCACGGCA TCAACCACAG CTTCGCGCTG ACACTGTTTG GTTACGGAAA GACGGAGCTC 480

CTGGGCAAGA ATATCACTTT CCTGATTCCT GGTTTCTACA GCTACATGGA CCTTGCGTAC 540

AACAGCTCAT TACAGCTCCC AGACCTGGCC AGCTGCCTGG ACGTCGGCAA TGAGAGTGGG 600

TGTGGGGAGA GAACCTTGGA CCCGTGGCAG GGCCAGGACC CAGCTGAGGG GGGCCAGGAT 660

CCAAGGATTA ATGTCGTGCT TGCTGGTGGC CACGTTGTGC CCCGAGATGA GATCCGGAAG 720

CTGATGGAAA GCCAAGACAT CTTCACCGGG ACTCAGACTG AGCTGATTGC TGGAGGCCAG 780

CTCCTTTCCT GCCTCTCACC TCAGCCTGCT CCAGGGGTGG ACAATGTCCC AGAAGGAAGC 840

CTGCCAGTGC ACGGTGAACA GGCGCTGCCC AAGGACCAGC AAATCACTGC CTTGGGGAGA 900

GAGGAACCTG TGGCAATAGA GAGCCCCGGA CAGGATCTTC TGGGAGAAAG CAGGTCTGAA 960

CCAGTGGATG TGAAGCCATT TGCTTCCTGC GAAGATTCTG AAGCTCCAGT CCCAGCTGAG 1020

GATGGGGGCA GTGATGCTGG CATGTGTGGC CTGTGTCAGA AGGCCCAGCT AGAGCGGATG 1080

GGAGTCAGTG GTCCCAGCGG TTCAGACCTT TGGGCTGGGG CTGCCGTGGC CAAGCCCCAG 1140

GCCAAGGGTC AGCTGGCGGG GGGCAGCCTC CTGATGCACT GCCCTTGCTA TGGGAGTGAA 1200

TGGGGCTTGT GGTGGCGAAG CCAGGACTTG GCCCCCAGCC CCTCTGGGAT GGCAGGCCTC 1260

TCGTTTGGGA CACCTACTCT AGATGAGCCG TGGCTGGGAG TGGAAAACGA CCGAGAAGAG 1320

CTGCAGACCT GCTTGATTAA GGAGCAGCTG TCCCAGTTGA GCCTTGCAGG AGCCCTGGAT 1380

GTCCCCCACG CCGAACTCGT TCCGACAGAG TGCCAGGCTG TCACCGCTCC TGTGTCGTCC 1440

TGCGATCTGG GAGGCAGAGA CCTGTGCGGT GGCTGCACGG GCAGCTCCTC AGCCTGCTAT 1500

GCCTTGGCCA CGGACCTCCC TGGGGGCCTG GAAGCAGTGG AGGCCCAGGA GGTTGATGTG 1560

AATTCGTTTT CCTGGAACCT CAAGGAACTC TTTTTCAGTG ACCAGACAGA CCAAACGTCA 1620

TCAAATTGTT CCTGTGCTAC GTCTGAACTC AGAGAGACAC CCTCTTCCTT GGCAGTGGGC 1680

TCCGATCCAG ATGTAGGCAG TCTCCAGGAA CAGGGGTCGT GTGTCCTGGA TGACAGGGAG 1740

CTGTTACTAC TGACCGGCAC CTGTGTTGAC CTTGGCCAAG GCCGACGGTT CCGGGAGAGC 1800

TGTGTGGGAC ATGATCCAAC AGAACCGCTT GAGGTTTGTT TGGTGTCCTC TGAGCATTAT 1860

GCAGCAAGCG ACAGAGAAAG CCCAGGACAC GTTCCTTCCA CGTTGGATGC TGGCCCTGAG 1920

GACACGTGCC CATCAGCAGA GGAGCCAAGG CTGAACGTCC AGGTCACCTC CACGCCCGTG 1980

ATCGTGATGC GCGGGGCTGC TGGCCTGCAG CGGGAGATCC AGGAGGGTGC CTACTCCGGG 2040

AGCTGCTACC ATCGAGATGG CTTACGGCTG AGTATACAGT TTGAGGTGAG GCGGGTGGAG 2100

CTCCAGGGCC CCACACCTCT GTTCTGCTGC TGGCTGGTGA AAGACCTCCT CCACAGCCAA 2160

CGCGACTCAG CCGCCAGGAC CCGCCTGTTC CTTGCCAGCC TGCCCGGCTC CACCCACTCT 2220

ACCGCTGCTG AGCTCACCGG ACCCAGCCTG GTGGAAGTGC TCAGAGCCAG ACCCTGGTTT 2280

GAGGAGCCCC CCAAGGCTGT GGAACTGGAG GGGTTGGCGG CCTGTGAGGG CGAGTACTCC 2340

CAAAAGTACA GTACCATGAG CCCGCTGGGC AGTGGGGCCT TCGGCTTCGT GTGGACTGCT 2400

GTGGACAAGG AAAAAAACAA GGAGGTGGTG GTGAAGTTTA TTAAGAAGGA GAAGGTCTTG 2460

GAGGATTGTT GGATTGAGGA TCCCAAACTT GGGAAAGTTA CTTTAGAGAT CGCAATTCTA 2520

TCCAGGGTGG AGCACGCCAA TATCATCAAG GTATTGGATA TATTTGAAAA CCAAGGGTTC 2580

TTCCAGCTTG TGATGGAGAA GCACGGCTCC GGCCTAGACC TCTTCGCTTT CATCGACCGC 2640

CACCCCAGGC TGGATGAGCC CCTGGCGAGC TACATCTTCC GACAACTAGT GTCAGCAGTG 2700

GGATACCTGC GCTTGAAGGA CATCATCCAC CGTGACATCA AGGATGAGAA CATCGTGATC 2760

GCCGAGGACT TCACAATCAA GCTGATAGAC TTTGGCTCGG CCGCCTACTT GGAAAGGGGA 2820

AAATTATTTT ATACTTTTTG TGGGACCATC GAGTACTGTG CACCGGAAGT TCTCATGGGG 2880

AATCCCTACA GAGGGCCGGA GCTGGAGATG TGGTCTCTGG GAGTCACTCT GTACACGCTG 2940

GTCTTTGAGG AGAACCCCTT CTGTGAGCTG GAGGAGACCG TGGAGGCTGC CATACACCCG 3000

CCATACCTGG TGTCCAAAGA ACTCATGAGC CTTGTGTCTG GGCTGCTGCA GCCAGTCCCT 3060

GAGAGACGCA CCACCTTGGA GAAGCTGGTG ACAGACCCGT GGGTAACACA GCCTGTGAAT 3120

CTTGCTGACT ATACATGGGA AGAGGTGTTT CGAGTAAACA AGCCAGAAAG TGGAGTTCTG 3180

TCCGCTGCGA GCCTGGAGAT GGGGAACAGG AGCCTGAGTG ATGTGGCCCA GGCTCAGGAG 3240

CTTTGTGGGG GCCCCGTTCC AGGCGAGGCT CCTAATGGCC AAGGCTGTTT GCATCCCGGG 3300

GATCCCCGTC TGCTGACCAG CTAA 3324

Domain Profile

S: 1     ykllktlGegsfgkVklakhketkekvavKiikkkkl......seeslekikrEieilkk  54

         y++++ lG+g+fg V +a++ke++++v+vK+ikk+k+      ++ +l k++ Ei+il++

Q: 783   YSTMSPLGSGAFGFVWTAVDKEKNKEVVVKFIKKEKVledcwiEDPKLGKVTLEIAILSR  842

         78899*******************************99999998899*************

S: 55    lkhpniikllevietkkklylvle.yasggeLfdyieekgrlkekearklfkqlvsavkY  113

         ++h niik+l+++e++ +++lv+e + sg +Lf  i ++ rl+e  a+ +f+qlvsav Y

Q: 843   VEHANIIKVLDIFENQGFFQLVMEkHGSGLDLFAFIDRHPRLDEPLASYIFRQLVSAVGY  902

         ************************888899******************************

S: 114   lhskgivHrDlKpeNllldkkenikiaDFGlanlfkegklletfcGsppYaaPellkgkk  173

         l+ k+i+HrD+K eN+++ ++ +ik++DFG a+++++gkl+ tfcG+++Y+aPe+l+g++

Q: 903   LRLKDIIHRDIKDENIVIAEDFTIKLIDFGSAAYLERGKLFYTFCGTIEYCAPEVLMGNP  962

         ************************************************************

S: 174   YegpkvDvWslGvvLyalvtgklPfdeenlkellekilkgkleipkklskelesLlrklL  233

         Y+gp++++WslGv+Ly+lv+++ Pf e       e++ +  ++ p+ +skel+sL++ lL

Q: 963   YRGPELEMWSLGVTLYTLVFEENPFCEL------EETVEAAIHPPYLVSKELMSLVSGLL  1016

         *************************998......788899999*****************

S: 234   vvdpekRatieeilkhewl  252

         +  pe+R+t+e++++++w+

Q: 1017  QPVPERRTTLEKLVTDPWV  1035

         ******************7

Domain Sequence
(FASTA)

YSTMSPLGSG AFGFVWTAVD KEKNKEVVVK FIKKEKVLED CWIEDPKLGK VTLEIAILSR 60

VEHANIIKVL DIFENQGFFQ LVMEKHGSGL DLFAFIDRHP RLDEPLASYI FRQLVSAVGY 120

LRLKDIIHRD IKDENIVIAE DFTIKLIDFG SAAYLERGKL FYTFCGTIEY CAPEVLMGNP 180

YRGPELEMWS LGVTLYTLVF EENPFCELEE TVEAAIHPPY LVSKELMSLV SGLLQPVPER 240

RTTLEKLVTD PWV 253

Keyword

3D-structure; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Kinase; Lipid-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005794	; C:Golgi apparatus
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0035091	; F:phosphatidylinositol binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0004871	; F:signal transducer activity
GO:0045719	; P:negative regulation of glycogen biosynthetic process
GO:0045727	; P:positive regulation of translation
GO:0046777	; P:protein autophosphorylation
GO:2000505	; P:regulation of energy homeostasis
GO:0070092	; P:regulation of glucagon secretion
GO:0043576	; P:regulation of respiratory gaseous exchange

KEGG

hsa:23178

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000014	; PAS.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00091	; PAS; 2.
SM00220	; S_TKc; 1.

Prosites

PS50112	; PAS; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013