EKS-HOS-00119

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00119

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/NAK	395.6	5.6E-118	46	309	264

Status

Reviewed

Ensembl Protein

ENSP00000386342

UniProt Accession

Q2M2I8; Q4ZFZ3; Q53RX6; Q9UPV4;

Protein Name

AP2-associated protein kinase 1

Protein Synonyms/Alias

Adaptor-associated kinase 1;

Gene Name

AAK1

Gene Synonyms/Alias

AAK1; KIAA1048;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000115977	ENSP00000386342	ENST00000409068
ENSG00000115977	ENSP00000385181	ENST00000406297
ENSG00000115977	ENSP00000386456	ENST00000409085

Organism

Homo sapiens

Functional Description

Regulates clathrin-mediated endocytosis byphosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.

Protein Length

674

Protein Sequence
(FASTA)

MKKFFDSRRE QGGSGLGSGS SGGGGSTSGL GSGYIGRVFG IGRQQVTVDE VLAEGGFAIV 60

FLVRTSNGMK CALKRMFVNN EHDLQVCKRE IQIMRDLSGH KNIVGYIDSS INNVSSGDVW 120

EVLILMDFCR GGQVVNLMNQ RLQTGFTENE VLQIFCDTCE AVARLHQCKT PIIHRDLKVE 180

NILLHDRGHY VLCDFGSATN KFQNPQTEGV NAVEDEIKKY TTLSYRAPEM VNLYSGKIIT 240

TKADIWALGC LLYKLCYFTL PFGESQVAIC DGNFTIPDNS RYSQDMHCLI RYMLEPDPDK 300

RPDIYQVSYF SFKLLKKECP IPNVQNSPIP AKLPEPVKAS EAAAKKTQPK ARLTDPIPTT 360

ETSIAPRQRP KAGQTQPNPG ILPIQPALTP RKRATVQPPP QAAGSSNQPG LLASVPQPKP 420

QAPPSQPLPQ TQAKQPQAPP TPQQTPSTQA QGLPAQAQAT PQHQQQLFLK QQQQQQQPPP 480

AQQQPAGTFY QQQQAQTQQF QAVHPATQKP AIAQFPVVSQ GGSQQQLMQN FYQQQQQQQQ 540

QQQQQQLATA LHQQQLMTQQ AALQQKPTMA AGQQPQPQPA AAPQPAPAQE PAIQAPVRQQ 600

PKVQTTPPPA VQGQKVGSLT PPSSPKTQRA GHRRILSDVT HSAVFGVPAS KSTQLLQAAA 660

AEAELLDPGR QTLQ 674

Nucleotide Sequence
(FASTA)

ATGAAGAAGT TTTTCGACTC CCGGCGAGAG CAGGGCGGCT CTGGCCTGGG CTCCGGCTCC 60

AGCGGAGGAG GGGGCAGCAC CTCGGGCCTG GGCAGTGGCT ACATCGGAAG AGTCTTCGGC 120

ATCGGGCGAC AGCAGGTCAC AGTGGACGAG GTGTTGGCGG AAGGTGGATT TGCTATTGTA 180

TTTCTGGTGA GGACAAGCAA TGGGATGAAA TGTGCCTTGA AACGCATGTT TGTCAACAAT 240

GAGCATGATC TCCAGGTGTG CAAGAGAGAA ATCCAGATAA TGAGGGATCT TTCAGGGCAC 300

AAGAATATTG TGGGTTACAT TGATTCTAGT ATCAACAACG TGAGTAGCGG TGATGTATGG 360

GAAGTGCTCA TTCTGATGGA CTTTTGTAGA GGTGGCCAGG TGGTAAACCT GATGAACCAG 420

CGCCTGCAAA CAGGCTTTAC AGAGAATGAA GTGCTCCAGA TATTTTGTGA TACCTGTGAA 480

GCTGTTGCCC GCCTGCATCA GTGCAAAACT CCTATTATCC ACCGGGACCT GAAGGTTGAA 540

AACATCCTCT TGCATGACCG AGGCCACTAT GTCCTGTGTG ACTTTGGAAG CGCCACCAAC 600

AAATTCCAGA ATCCACAAAC TGAGGGAGTC AATGCAGTAG AAGATGAGAT TAAGAAATAC 660

ACAACGCTGT CCTATCGAGC ACCAGAAATG GTCAACCTGT ACAGTGGCAA AATCATCACT 720

ACGAAGGCAG ACATTTGGGC TCTTGGATGT TTGTTGTATA AATTATGCTA CTTCACTTTG 780

CCATTTGGGG AAAGTCAGGT GGCAATTTGT GATGGAAACT TCACAATTCC TGATAATTCT 840

CGATATTCTC AAGACATGCA CTGCCTAATT AGGTATATGT TGGAACCAGA CCCTGACAAA 900

AGGCCGGATA TTTACCAGGT GTCCTACTTC TCATTTAAGC TACTCAAGAA AGAGTGCCCA 960

ATTCCAAATG TACAGAACTC TCCCATTCCT GCAAAGCTTC CTGAACCAGT GAAAGCCAGT 1020

GAGGCAGCTG CAAAAAAGAC CCAGCCAAAG GCCAGACTGA CAGATCCCAT TCCCACCACA 1080

GAGACTTCAA TTGCACCCCG CCAGAGGCCT AAAGCTGGGC AGACTCAGCC GAACCCAGGA 1140

ATCCTTCCCA TCCAGCCAGC GCTGACACCC CGGAAGAGGG CCACTGTTCA GCCCCCACCT 1200

CAGGCTGCAG GATCCAGCAA TCAGCCTGGC CTTTTAGCCA GTGTTCCCCA ACCAAAACCC 1260

CAAGCCCCAC CCAGCCAGCC TCTGCCGCAA ACTCAGGCCA AGCAGCCACA GGCTCCTCCC 1320

ACTCCACAGC AGACGCCTTC TACTCAGGCC CAGGGTCTGC CCGCTCAGGC CCAGGCCACA 1380

CCCCAGCACC AGCAGCAACT CTTCCTCAAG CAGCAACAGC AGCAGCAACA GCCACCGCCA 1440

GCACAGCAGC AGCCGGCAGG CACGTTTTAC CAGCAGCAGC AGGCCCAGAC TCAGCAGTTT 1500

CAGGCAGTAC ATCCAGCAAC CCAGAAACCA GCAATTGCTC AGTTCCCTGT GGTGTCCCAA 1560

GGAGGCTCTC AACAGCAGCT AATGCAGAAT TTCTACCAGC AGCAGCAGCA GCAGCAACAA 1620

CAACAGCAAC AGCAACAGCT GGCCACAGCC CTGCATCAAC AACAGCTGAT GACTCAGCAG 1680

GCTGCCTTGC AGCAAAAGCC CACTATGGCA GCAGGACAGC AGCCCCAGCC ACAGCCAGCT 1740

GCAGCCCCAC AGCCAGCCCC TGCCCAGGAG CCAGCGATTC AAGCCCCAGT AAGACAACAG 1800

CCAAAGGTTC AGACAACCCC ACCTCCTGCC GTCCAGGGGC AGAAAGTTGG ATCTCTCACT 1860

CCACCCTCAT CCCCCAAAAC CCAACGTGCT GGGCACAGGC GTATTCTCAG TGACGTAACC 1920

CACAGTGCAG TCTTTGGGGT CCCTGCCAGC AAATCAACCC AGCTGCTCCA GGCAGCTGCA 1980

GCTGAGGCAG AATTGCTTGA CCCAGGGAGG CAGACTTTGC AGTGA 2025

Domain Profile

S: 1     vriekvlaeGGfalvylaedastgekyalKrilvneeedleeikrEieimkklsghkniv  60

         v++++vlaeGGfa+v+l++ +s+g k+alKr++vn+e+dl+ +krEi+im++lsghkniv

Q: 46    VTVDEVLAEGGFAIVFLVR-TSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIV  104

         6899***************.99**************************************

S: 61    klvdssvkeekdngskevlllleyckkgslvdllnkrlekgelseaeilkilldvtrava  120

          ++dss+++ ++++++evl+l+++c+ g++v+l+n+rl++g ++e+e+l+i++d+++ava

Q: 105   GYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTG-FTENEVLQIFCDTCEAVA  163

         ************9***************************9.******************

S: 121   alHalsvpliHRDlkveNillsdegelvlcDlGSattaileveskqelsalqeeiekntt  180

         +lH++++p+iHRDlkveNill+d+g++vlcD+GSat+++++++ +++++a+++ei+k+tt

Q: 164   RLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQ-TEGVNAVEDEIKKYTT  222

         *******************************************.9***************

S: 181   lsyrsPElidlysnlvideksDiWaLGcllYklcffesPfeesvklailngkysiPensr  240

         lsyr+PE+++lys+++i++k+DiWaLGcllYklc+f+ Pf+es ++ai++g+++iP+nsr

Q: 223   LSYRAPEMVNLYSGKIITTKADIWALGCLLYKLCYFTLPFGES-QVAICDGNFTIPDNSR  281

         *******************************************.****************

S: 241   yseklkslikkmlevdpeeRptiaqvle  268

         ys+++++li++mle+dp++Rp+i+qv++

Q: 282   YSQDMHCLIRYMLEPDPDKRPDIYQVSY  309

         **************************87

Domain Sequence
(FASTA)

VTVDEVLAEG GFAIVFLVRT SNGMKCALKR MFVNNEHDLQ VCKREIQIMR DLSGHKNIVG 60

YIDSSINNVS SGDVWEVLIL MDFCRGGQVV NLMNQRLQTG FTENEVLQIF CDTCEAVARL 120

HQCKTPIIHR DLKVENILLH DRGHYVLCDF GSATNKFQNP QTEGVNAVED EIKKYTTLSY 180

RAPEMVNLYS GKIITTKADI WALGCLLYKL CYFTLPFGES QVAICDGNFT IPDNSRYSQD 240

MHCLIRYMLE PDPDKRPDIY QVSY 264

Keyword

Alternative splicing; ATP-binding; Cell membrane; Coated pit; Complete proteome; Endocytosis; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0031252	; C:cell leading edge
GO:0071439	; C:clathrin complex
GO:0030136	; C:clathrin-coated vesicle
GO:0005905	; C:coated pit
GO:0019897	; C:extrinsic to plasma membrane
GO:0043195	; C:terminal button
GO:0035612	; F:AP-2 adaptor complex binding
GO:0005524	; F:ATP binding
GO:0005112	; F:Notch binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0006897	; P:endocytosis
GO:0045747	; P:positive regulation of Notch signaling pathway
GO:0046777	; P:protein autophosphorylation
GO:0050821	; P:protein stabilization
GO:2000369	; P:regulation of clathrin-mediated endocytosis
GO:0032880	; P:regulation of protein localization

KEGG

hsa:22848

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013