EKS-HOS-00249

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00249

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CAMK/CAMKL	386.8	2.0E-115	60	311	252

Status

Reviewed

Ensembl Protein

ENSP00000355884

UniProt Accession

Q9P0L2; D3DTB0; D3DTB1; Q2HIY1; Q5VTF9; Q5VTG0; Q96SW9; Q9P251;

Protein Name

Serine/threonine-protein kinase MARK1

Protein Synonyms/Alias

MAP/microtubule affinity-regulating kinase 1; PAR1 homolog c; Par-1c; Par1c;

Gene Name

MARK1

Gene Synonyms/Alias

MARK1; KIAA1477, MARK;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000116141	ENSP00000355885	ENST00000366918
ENSG00000116141	ENSP00000355884	ENST00000366917
ENSG00000116141	ENSP00000386017	ENST00000402574

Organism

Homo sapiens

Functional Description

Serine/threonine-protein kinase involved in cellpolarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2, MAP4 and MAPT/TAU. Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3).

Protein Length

795

Protein Sequence
(FASTA)

MSARTPLPTV NERDTENHTS VDGYTEPHIQ PTKSSSRQNI PRCRNSITSA TDEQPHIGNY 60

RLQKTIGKGN FAKVKLARHV LTGREVAVKI IDKTQLNPTS LQKLFREVRI MKILNHPNIV 120

KLFEVIETEK TLYLVMEYAS GGEVFDYLVA HGRMKEKEAR AKFRQIVSAV QYCHQKYIVH 180

RDLKAENLLL DGDMNIKIAD FGFSNEFTVG NKLDTFCGSP PYAAPELFQG KKYDGPEVDV 240

WSLGVILYTL VSGSLPFDGQ NLKELRERVL RGKYRIPFYM STDCENLLKK LLVLNPIKRG 300

SLEQIMKDRW MNVGHEEEEL KPYTEPDPDF NDTKRIDIMV TMGFARDEIN DALINQKYDE 360

VMATYILLGR KPPEFEGGES LSSGNLCQRS RPSSDLNNST LQSPAHLKVQ RSISANQKQR 420

RFSDHAGPSI PPAVSYTKRP QANSVESEQK EEWDKDVARK LGSTTVGSKS EMTASPLVGP 480

ERKKSSTIPS NNVYSGGSMA RRNTYVCERT TDRYVALQNG KDSSLTEMSV SSISSAGSSV 540

ASAVPSARPR HQKSMSTSGH PIKVTLPTIK DGSEAYRPGT TQRVPAASPS AHSISTATPD 600

RTRFPRGSSS RSTFHGEQLR ERRSVAYNGP PASPSHETGA FAHARRGTST GIISKITSKF 660

VRRDPSEGEA SGRTDTSRST SGEPKERDKE EGKDSKPRSL RFTWSMKTTS SMDPNDMMRE 720

IRKVLDANNC DYEQKERFLL FCVHGDARQD SLVQWEMEVC KLPRLSLNGV RFKRISGTSI 780

AFKNIASKIA NELKL 795

Nucleotide Sequence
(FASTA)

ATGTCGGCCC GGACGCCATT GCCGACGGTG AACGAGCGGG ACACGGAAAA TCATACATCT 60

GTGGATGGAT ATACTGAACC ACACATCCAG CCTACCAAGT CGAGTAGCAG ACAGAACATC 120

CCCCGGTGTA GAAACTCCAT TACGTCAGCA ACAGATGAAC AGCCTCACAT TGGAAATTAC 180

CGTTTACAAA AAACAATAGG GAAGGGAAAT TTTGCCAAAG TCAAATTGGC AAGACACGTT 240

CTAACTGGTA GAGAGGTTGC TGTGAAAATA ATAGACAAAA CTCAGCTAAA TCCTACCAGT 300

CTACAAAAGT TATTTCGAGA AGTACGAATA ATGAAGATAC TGAATCATCC TAATATAGTA 360

AAATTGTTTG AAGTTATTGA AACAGAGAAG ACTCTCTATT TAGTCATGGA ATACGCGAGT 420

GGGGGTGAAG TATTTGATTA CTTAGTTGCC CATGGAAGAA TGAAAGAGAA AGAGGCCCGT 480

GCAAAATTTA GGCAGATTGT ATCTGCTGTA CAGTATTGTC ATCAAAAGTA CATTGTTCAC 540

CGTGATCTTA AGGCTGAAAA CCTTCTCCTT GATGGTGATA TGAATATTAA AATTGCTGAC 600

TTTGGTTTTA GTAATGAATT TACAGTTGGG AACAAATTGG ACACATTTTG TGGAAGCCCA 660

CCCTATGCTG CTCCCGAGCT TTTCCAAGGA AAGAAGTATG ATGGGCCTGA AGTGGATGTG 720

TGGAGTCTGG GCGTCATTCT CTATACATTA GTCAGTGGCT CCTTGCCTTT CGATGGCCAG 780

AATTTAAAGG AACTGCGAGA GCGAGTTTTA CGAGGGAAGT ACCGTATTCC CTTCTATATG 840

TCCACAGACT GTGAAAATCT TCTGAAGAAA TTATTAGTCC TGAATCCAAT AAAGAGAGGC 900

AGCTTGGAAC AAATAATGAA AGATCGATGG ATGAATGTTG GTCATGAAGA GGAAGAACTA 960

AAGCCATATA CTGAGCCTGA TCCGGATTTC AATGACACAA AAAGAATAGA CATTATGGTC 1020

ACCATGGGCT TTGCACGAGA TGAAATAAAT GATGCCTTAA TAAATCAGAA GTATGATGAA 1080

GTTATGGCTA CTTATATTCT TCTAGGTAGA AAACCACCTG AATTTGAAGG TGGTGAATCG 1140

TTATCCAGTG GAAACTTGTG TCAGAGGTCC CGGCCCAGTA GTGACTTAAA CAACAGCACT 1200

CTTCAGTCCC CTGCTCACCT GAAGGTCCAG AGAAGTATCT CAGCAAATCA GAAGCAGCGG 1260

CGTTTCAGTG ATCATGCTGG TCCATCCATT CCTCCTGCTG TATCATATAC CAAAAGACCT 1320

CAGGCTAACA GTGTGGAAAG TGAACAGAAA GAGGAGTGGG ACAAAGATGT GGCTCGAAAA 1380

CTTGGCAGCA CAACAGTTGG ATCAAAAAGC GAGATGACTG CAAGCCCTCT TGTAGGGCCA 1440

GAGAGGAAAA AATCTTCAAC TATTCCAAGT AACAATGTGT ATTCTGGAGG TAGCATGGCA 1500

AGAAGGAATA CATATGTCTG TGAAAGGACC ACAGATCGAT ACGTAGCATT GCAGAATGGA 1560

AAAGACAGCA GCCTTACGGA GATGTCTGTG AGTAGCATAT CTTCTGCAGG CTCTTCTGTG 1620

GCCTCTGCTG TCCCCTCAGC ACGACCCCGC CACCAGAAGT CCATGTCCAC TTCTGGTCAT 1680

CCTATTAAAG TCACACTGCC AACCATTAAA GACGGCTCTG AAGCTTACCG GCCTGGTACA 1740

ACCCAGAGAG TGCCTGCTGC TTCCCCATCT GCTCACAGTA TTAGTACTGC GACTCCAGAC 1800

CGGACCCGTT TTCCCCGAGG GAGCTCAAGC CGAAGCACTT TCCATGGTGA ACAGCTCCGG 1860

GAGCGACGCA GCGTTGCTTA TAATGGGCCA CCTGCTTCAC CATCCCATGA AACGGGTGCA 1920

TTTGCACATG CCAGAAGGGG AACGTCAACT GGTATAATAA GCAAAATCAC ATCCAAATTT 1980

GTTCGCAGGG ATCCAAGTGA AGGCGAAGCC AGTGGCAGAA CCGACACCTC AAGAAGTACA 2040

TCAGGGGAAC CAAAAGAAAG AGACAAGGAA GAGGGTAAAG ATTCTAAGCC GCGTTCTTTG 2100

CGGTTCACAT GGAGTATGAA GACCACTAGT TCAATGGACC CTAATGACAT GATGAGAGAA 2160

ATCCGAAAAG TGTTAGATGC AAATAACTGT GATTATGAGC AAAAAGAGAG ATTTTTGCTT 2220

TTCTGTGTCC ATGGAGACGC TAGACAGGAT AGCCTCGTGC AGTGGGAGAT GGAAGTCTGC 2280

AAGTTGCCAC GACTGTCACT TAATGGGGTT CGCTTCAAGC GAATATCTGG GACATCTATT 2340

GCCTTTAAGA ACATTGCATC AAAAATAGCA AATGAGCTTA AGCTGTAA 2388

Domain Profile

S: 1     ykllktlGegsfgkVklakhketkekvavKiikkkklseeslekikrEieilkklkhpni  60

         y+l+kt+G+g+f+kVkla+h +t+++vavKii+k++l+ +sl+k++rE++i+k+l+hpni

Q: 60    YRLQKTIGKGNFAKVKLARHVLTGREVAVKIIDKTQLNPTSLQKLFREVRIMKILNHPNI  119

         99**********************************************************

S: 61    ikllevietkkklylvleyasggeLfdyieekgrlkekearklfkqlvsavkYlhskgiv  120

         +kl+eviet+k+lylv+eyasgge+fdy++++gr+kekear+ f+q+vsav+Y+h+k iv

Q: 120   VKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKYIV  179

         ************************************************************

S: 121   HrDlKpeNllldkkenikiaDFGlanlfkegklletfcGsppYaaPellkgkkYegpkvD  180

         HrDlK+eNllld ++nikiaDFG++n+f+ g++l+tfcGsppYaaPel++gkkY+gp+vD

Q: 180   HRDLKAENLLLDGDMNIKIADFGFSNEFTVGNKLDTFCGSPPYAAPELFQGKKYDGPEVD  239

         ************************************************************

S: 181   vWslGvvLyalvtgklPfdeenlkellekilkgkleipkklskelesLlrklLvvdpekR  240

         vWslGv+Ly+lv+g+lPfd++nlkel+e++l+gk++ip+++s+++e+Ll+klLv +p kR

Q: 240   VWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKLLVLNPIKR  299

         ************************************************************

S: 241   atieeilkhewl  252

          ++e+i+k++w+

Q: 300   GSLEQIMKDRWM  311

         ***********8

Domain Sequence
(FASTA)

YRLQKTIGKG NFAKVKLARH VLTGREVAVK IIDKTQLNPT SLQKLFREVR IMKILNHPNI 60

VKLFEVIETE KTLYLVMEYA SGGEVFDYLV AHGRMKEKEA RAKFRQIVSA VQYCHQKYIV 120

HRDLKAENLL LDGDMNIKIA DFGFSNEFTV GNKLDTFCGS PPYAAPELFQ GKKYDGPEVD 180

VWSLGVILYT LVSGSLPFDG QNLKELRERV LRGKYRIPFY MSTDCENLLK KLLVLNPIKR 240

GSLEQIMKDR WM 252

Keyword

3D-structure; Alternative splicing; ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Lipid-binding; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase; Wnt signaling pathway.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Tetraodon nigroviridis"; ?>Tupaia belangeri"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0015630	; C:microtubule cytoskeleton
GO:0005886	; C:plasma membrane
GO:0005524	; F:ATP binding
GO:0000287	; F:magnesium ion binding
GO:0070300	; F:phosphatidic acid binding
GO:0005546	; F:phosphatidylinositol-4,5-bisphosphate binding
GO:0001786	; F:phosphatidylserine binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0050321	; F:tau-protein kinase activity
GO:0007010	; P:cytoskeleton organization
GO:0007243	; P:intracellular protein kinase cascade
GO:0000226	; P:microtubule cytoskeleton organization
GO:0001764	; P:neuron migration
GO:0016055	; P:Wnt receptor signaling pathway

KEGG

hsa:4139

;

InterPros

IPR001772	; Kinase-assoc_KA1.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.
IPR015940	; UBA/transl_elong_EF1B_N_euk.

Pfam

PF02149	; KA1; 1.
PF00069	; Pkinase; 1.

SMARTs

SM00220	; S_TKc; 1.
SM00165	; UBA; 1.

Prosites

PS50032	; KA1; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS50030	; UBA; 1.

Prints

Created Date

20-Feb-2013