EKS-HOS-00208

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00208

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
AGC/MAST	361.4	1.6E-107	35	310	276

Status

Reviewed

Ensembl Protein

ENSP00000343446

UniProt Accession

Q96GX5; Q5T8D5; Q5T8D7; Q8NCD6; Q96SJ5;

Protein Name

Serine/threonine-protein kinase greatwall

Protein Synonyms/Alias

GW; GWL; hGWL; Microtubule-associated serine/threonine-protein kinase-like; MAST-L;

Gene Name

MASTL

Gene Synonyms/Alias

MASTL; GW, GWL, THC2;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000120539	ENSP00000343446	ENST00000342386
ENSG00000120539	ENSP00000365113	ENST00000375946
ENSG00000120539	ENSP00000365107	ENST00000375940

Organism

Homo sapiens

Functional Description

Serine/threonine kinase that plays a key role in M phaseby acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation.

Protein Length

840

Protein Sequence
(FASTA)

MDPTAGSKKE PGGGAATEEG VNRIAVPKPP SIEEFSIVKP ISRGAFGKVY LGQKGGKLYA 60

VKVVKKADMI NKNMTHQVQA ERDALALSKS PFIVHLYYSL QSANNVYLVM EYLIGGDVKS 120

LLHIYGYFDE EMAVKYISEV ALALDYLHRH GIIHRDLKPD NMLISNEGHI KLTDFGLSKV 180

TLNRDINMMD ILTTPSMAKP RQDYSRTPGQ VLSLISSLGF NTPIAEKNQD PANILSACLS 240

ETSQLSQGLV CPMSVDQKDT TPYSSKLLKS CLETVASNPG MPVKCLTSNL LQSRKRLATS 300

SASSQSHTFI SSVESECHSS PKWEKDCQES DEALGPTMMS WNAVEKLCAK SANAIETKGF 360

NKKDLELALS PIHNSSALPT TGRSCVNLAK KCFSGEVSWE AVELDVNNIN MDTDTSQLGF 420

HQSNQWAVDS GGISEEHLGK RSLKRNFELV DSSPCKKIIQ NKKTCVEYKH NEMTNCYTNQ 480

NTGLTVEVQD LKLSVHKSQQ NDCANKENIV NSFTDKQQTP EKLPIPMIAK NLMCELDEDC 540

EKNSKRDYLS SSFLCSDDDR ASKNISMNSD SSFPGISIME SPLESQPLDS DRSIKESSFE 600

ESNIEDPLIV TPDCQEKTSP KGVENPAVQE SNQKMLGPPL EVLKTLASKR NAVAFRSFNS 660

HINASNNSEP SRMNMTSLDA MDISCAYSGS YPMAITPTQK RRSCMPHQTP NQIKSGTPYR 720

TPKSVRRGVA PVDDGRILGT PDYLAPELLL GRAHDIPWPE GEEKLSDNAQ SAVEILLTID 780

DTKRAGMKEL KRHPLFSDVD WENLQHQTMP FIPQPDDETD TSYFEARNTA QHLTVSGFSL 840

Nucleotide Sequence
(FASTA)

ATGGATCCCA CCGCGGGAAG CAAGAAGGAG CCTGGAGGAG GCGCGGCGAC TGAGGAGGGC 60

GTGAATAGGA TCGCAGTGCC AAAACCGCCC TCCATTGAGG AATTCAGCAT AGTGAAGCCC 120

ATTAGCCGGG GCGCCTTCGG GAAAGTGTAT CTGGGGCAGA AAGGCGGCAA ATTGTATGCA 180

GTAAAGGTTG TTAAAAAAGC AGACATGATC AACAAAAATA TGACTCATCA GGTCCAAGCT 240

GAGAGAGATG CACTGGCACT AAGCAAAAGC CCATTCATTG TCCATTTGTA TTATTCACTG 300

CAGTCTGCAA ACAATGTCTA CTTGGTAATG GAATATCTTA TTGGGGGAGA TGTCAAGTCT 360

CTCCTACATA TATATGGTTA TTTTGATGAA GAGATGGCTG TGAAATATAT TTCTGAAGTA 420

GCACTGGCTC TAGACTACCT TCACAGACAT GGAATCATCC ACAGGGACTT GAAACCGGAC 480

AATATGCTTA TTTCTAATGA GGGTCATATT AAACTGACGG ATTTTGGCCT TTCAAAAGTT 540

ACTTTGAATA GAGATATTAA TATGATGGAT ATCCTTACAA CACCATCAAT GGCAAAACCT 600

AGACAAGATT ATTCAAGAAC CCCAGGACAA GTGTTATCGC TTATCAGCTC GTTGGGATTT 660

AACACACCAA TTGCAGAAAA AAATCAAGAC CCTGCAAACA TCCTTTCAGC CTGTCTGTCT 720

GAAACATCAC AGCTTTCTCA AGGACTCGTA TGCCCTATGT CTGTAGATCA AAAGGACACT 780

ACGCCTTATT CTAGCAAATT ACTAAAATCA TGTCTTGAAA CAGTTGCCTC CAACCCAGGA 840

ATGCCTGTGA AGTGTCTAAC TTCTAATTTA CTCCAGTCTA GGAAAAGGCT GGCCACATCC 900

AGTGCCAGTA GTCAATCCCA CACCTTCATA TCCAGTGTGG AATCAGAATG CCACAGCAGT 960

CCCAAATGGG AAAAAGATTG CCAGGAAAGT GATGAAGCAT TGGGCCCAAC AATGATGAGT 1020

TGGAATGCAG TTGAAAAGTT ATGCGCAAAA TCTGCAAATG CCATTGAGAC GAAAGGTTTC 1080

AATAAAAAGG ATCTGGAGTT AGCTCTTTCT CCCATTCATA ACAGCAGTGC CCTTCCCACC 1140

ACTGGACGCT CTTGTGTAAA CCTTGCTAAA AAATGCTTCT CTGGGGAAGT TTCTTGGGAA 1200

GCAGTAGAAC TGGATGTAAA TAATATAAAT ATGGACACTG ACACAAGTCA GTTAGGTTTC 1260

CATCAGTCAA ATCAGTGGGC TGTGGATTCT GGTGGGATAT CTGAAGAGCA CCTTGGGAAA 1320

AGAAGTTTAA AAAGAAATTT TGAGTTGGTT GACTCCAGTC CTTGTAAAAA AATTATACAG 1380

AATAAAAAAA CTTGTGTAGA GTATAAGCAT AACGAAATGA CAAATTGTTA TACAAATCAA 1440

AATACAGGCT TAACAGTTGA AGTGCAGGAC CTTAAGCTAT CAGTGCACAA AAGTCAACAA 1500

AATGACTGTG CTAATAAGGA GAACATTGTC AATTCTTTTA CTGATAAACA ACAAACACCA 1560

GAAAAATTAC CTATACCAAT GATAGCAAAA AACCTTATGT GTGAACTCGA TGAAGACTGT 1620

GAAAAGAATA GTAAGAGGGA CTACTTAAGT TCTAGTTTTC TATGTTCTGA TGATGATAGA 1680

GCTTCTAAAA ATATTTCTAT GAACTCTGAT TCATCTTTTC CTGGAATTTC TATAATGGAA 1740

AGTCCATTAG AAAGTCAGCC CTTAGATTCA GATAGAAGCA TCAAAGAATC CTCTTTTGAA 1800

GAATCAAATA TTGAAGATCC ACTTATTGTA ACACCAGATT GCCAAGAAAA GACCTCACCA 1860

AAAGGTGTCG AGAACCCTGC TGTACAAGAG AGTAACCAAA AAATGTTAGG TCCTCCTTTG 1920

GAGGTGCTGA AAACGTTAGC CTCTAAAAGA AATGCTGTTG CTTTTCGAAG TTTTAACAGT 1980

CATATTAATG CATCCAATAA CTCAGAACCA TCCAGAATGA ACATGACTTC TTTAGATGCA 2040

ATGGATATTT CGTGTGCCTA CAGTGGTTCA TATCCCATGG CTATAACCCC TACTCAAAAA 2100

AGAAGATCCT GTATGCCACA TCAGACCCCA AATCAGATCA AGTCGGGAAC TCCATACCGA 2160

ACTCCGAAGA GTGTGAGAAG AGGGGTGGCC CCCGTTGATG ATGGGCGAAT TCTAGGAACC 2220

CCAGACTACC TTGCACCTGA GCTGTTACTA GGCAGGGCCC ATGATATCCC TTGGCCAGAA 2280

GGTGAAGAAA AGTTATCTGA TAATGCTCAA AGTGCAGTAG AAATACTTTT AACCATTGAT 2340

GATACAAAGA GAGCTGGAAT GAAAGAGCTA AAACGTCATC CTCTCTTCAG TGATGTGGAC 2400

TGGGAAAATC TGCAGCATCA GACTATGCCT TTCATCCCCC AGCCAGATGA TGAAACAGAT 2460

ACCTCCTATT TTGAAGCCAG GAATACTGCT CAGCACCTGA CTGTATCTGG ATTTAGTCTG 2520

TAG 2523

Domain Profile

S: 1     feiiklisrGayGavylvrhketrkrfavkkikkqdlilknqvqqvfaerdilalaenpf  60

         f+i+k+isrGa+G+vyl  +++++k++avk++kk+d+i+kn+++qv+aerd+lal+++pf

Q: 35    FSIVKPISRGAFGKVYL--GQKGGKLYAVKVVKKADMINKNMTHQVQAERDALALSKSPF  92

         89***************..*****************************************

S: 61    vvslyysletrrnlylvmeyveGGdvksllkniGaldvemarlyiaevvlaleylhryGi  120

         +v+lyysl++++n+ylvmey++GGdvksll+++G++d+ema++yi+ev+lal+ylhr+Gi

Q: 93    IVHLYYSLQSANNVYLVMEYLIGGDVKSLLHIYGYFDEEMAVKYISEVALALDYLHRHGI  152

         ************************************************************

S: 121   vhrdlkPdnllitseGhikltdfGlskvglmslttnlyegyidkdarefldkqvcgtPey  180

         +hrdlkPdn+li++eGhikltdfGlskv+l+          +d++++++l     +tP++

Q: 153   IHRDLKPDNMLISNEGHIKLTDFGLSKVTLN----------RDINMMDIL-----TTPSM  197

         *******************************..........********9.....*****

S: 181   iaPevilrqgygkpvdwwalgiilyeflvgivpffgdtpeelfaqv.....lsdeivwP.  234

         ++P    rq+y++++++++++i++++f+++i+++++d+++ l+a++     ls+++v+P

Q: 198   AKP----RQDYSRTPGQVLSLISSLGFNTPIAEKNQDPANILSACLsetsqLSQGLVCPm  253

         ***....*************************************9999999*********

S: 235   ...ekde.alssealelisellesnP..............rerlgtgsaaevkqhefl  274

            +kd+ ++ss++l+++ e+++snP              r+rl+t s+a++++h+f+

Q: 254   svdQKDTtPYSSKLLKSCLETVASNPgmpvkcltsnllqsRKRLAT-SSASSQSHTFI  310

         **9899889*************************************.88899***996

Domain Sequence
(FASTA)

FSIVKPISRG AFGKVYLGQK GGKLYAVKVV KKADMINKNM THQVQAERDA LALSKSPFIV 60

HLYYSLQSAN NVYLVMEYLI GGDVKSLLHI YGYFDEEMAV KYISEVALAL DYLHRHGIIH 120

RDLKPDNMLI SNEGHIKLTD FGLSKVTLNR DINMMDILTT PSMAKPRQDY SRTPGQVLSL 180

ISSLGFNTPI AEKNQDPANI LSACLSETSQ LSQGLVCPMS VDQKDTTPYS SKLLKSCLET 240

VASNPGMPVK CLTSNLLQSR KRLATSSASS QSHTFI 276

Keyword

Alternative splicing; ATP-binding; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Disease mutation; Kinase; Mitosis; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sorex araneus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005813	; C:centrosome
GO:0032154	; C:cleavage furrow
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0051721	; F:protein phosphatase 2A binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0051301	; P:cell division
GO:0000086	; P:G2/M transition of mitotic cell cycle
GO:0007067	; P:mitosis
GO:0034048	; P:negative regulation of protein phosphatase type 2A activity
GO:0051726	; P:regulation of cell cycle
GO:0006974	; P:response to DNA damage stimulus

KEGG

hsa:84930

;

InterPros

IPR000961	; AGC-kinase_C.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 2.

SMARTs

SM00133

; S_TK_X; 1.

Prosites

PS51285	; AGC_KINASE_CTER; 1.
PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013