EKS-HOS-00178

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00178

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
AGC/PKC	454.1	8.2E-136	351	614	264

Status

Reviewed

Ensembl Protein

ENSP00000263431

UniProt Accession

P05129;

Protein Name

Protein kinase C gamma type

Protein Synonyms/Alias

PKC-gamma;

Gene Name

PRKCG

Gene Synonyms/Alias

PRKCG; PKCG;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000126583	ENSP00000387919	ENST00000419486
ENSG00000126583	ENSP00000471271	ENST00000474397
ENSG00000126583	ENSP00000471544	ENST00000479081
ENSG00000126583	ENSP00000440541	ENST00000536044
ENSG00000126583	ENSP00000263431	ENST00000263431
ENSG00000126583	ENSP00000438090	ENST00000542049
ENSG00000126583	ENSP00000443493	ENST00000540413

Organism

Homo sapiens

Functional Description

Calcium-activated, phospholipid- and diacylglycerol(DAG)-dependent serine/threonine-protein kinase that plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GRIA4/GLUR4 and GRIN1/NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. Binds and phosphorylates GRIA4/GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. In primary cerebellar neurons treated with the agonist 3,5-dihyidroxyphenylglycine, functions downstream of the metabotropic glutamate receptor GRM5/MGLUR5 and phosphorylates GRIN1/NMDAR1 receptor which plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. May be involved in the regulation of hippocampal long-term potentiation (LTP), but may be not necessary for the process of synaptic plasticity. May be involved in desensitization of mu-type opioid receptor-mediated G-protein activation in the spinal cord, and may be critical for the development and/or maintenance of morphine-induced reinforcing effects in the limbic forebrain. May modulate the functionality of mu-type-opioid receptors by participating in a signaling pathway which leads to the phosphorylation and degradation of opioid receptors. May also contributes to chronic morphine-induced changes in nociceptive processing. Plays a role in neuropathic pain mechanisms and contributes to the maintenance of the allodynia pain produced by peripheral inflammation. Plays an important role in initial sensitivity and tolerance to ethanol, by mediating the behavioral effects of ethanol as well as the effects of this drug on the GABA(A) receptors. During and after cerebral ischemia modulate neurotransmission and cell survival in synaptic membranes, and is involved in insulin-induced inhibition of necrosis, an important mechanism for minimizing ischemic injury. Required for the elimination of multiple climbing fibers during innervation of Purkinje cells in developing cerebellum. Is activated in lens epithelial cells upon hydrogen peroxide treatment, and phosphorylates connexin-43 (GJA1/CX43), resulting in disassembly of GJA1 gap junction plaques and inhibition of gap junction activity which could provide a protective effect against oxidative stress (By similarity). Phosphorylates p53/TP53 and promotes p53/TP53-dependent apoptosis in response to DNA damage.

Protein Length

697

Protein Sequence
(FASTA)

MAGLGPGVGD SEGGPRPLFC RKGALRQKVV HEVKSHKFTA RFFKQPTFCS HCTDFIWGIG 60

KQGLQCQVCS FVVHRRCHEF VTFECPGAGK GPQTDDPRNK HKFRLHSYSS PTFCDHCGSL 120

LYGLVHQGMK CSCCEMNVHR RCVRSVPSLC GVDHTERRGR LQLEIRAPTA DEIHVTVGEA 180

RNLIPMDPNG LSDPYVKLKL IPDPRNLTKQ KTRTVKATLN PVWNETFVFN LKPGDVERRL 240

SVEVWDWDRT SRNDFMGAMS FGVSELLKAP VDGWYKLLNQ EEGEYYNVPV ADADNCSLLQ 300

KFEACNYPLE LYERVRMGPS SSPIPSPSPS PTDPKRCFFG ASPGRLHISD FSFLMVLGKG 360

SFGKVMLAER RGSDELYAIK ILKKDVIVQD DDVDCTLVEK RVLALGGRGP GGRPHFLTQL 420

HSTFQTPDRL YFVMEYVTGG DLMYHIQQLG KFKEPHAAFY AAEIAIGLFF LHNQGIIYRD 480

LKLDNVMLDA EGHIKITDFG MCKENVFPGT TTRTFCGTPD YIAPEIIAYQ PYGKSVDWWS 540

FGVLLYEMLA GQPPFDGEDE EELFQAIMEQ TVTYPKSLSR EAVAICKGFL TKHPGKRLGS 600

GPDGEPTIRA HGFFRWIDWE RLERLEIPPP FRPRPCGRSG ENFDKFFTRA APALTPPDRL 660

VLASIDQADF QGFTYVNPDF VHPDARSPTS PVPVPVM 697

Nucleotide Sequence
(FASTA)

ATGGCTGGTC TGGGCCCCGG CGTAGGCGAT TCAGAGGGGG GACCCCGGCC CCTGTTTTGC 60

AGAAAGGGGG CCCTGAGGCA GAAGGTGGTC CACGAAGTCA AGAGCCACAA GTTCACCGCT 120

CGCTTCTTCA AGCAGCCCAC CTTCTGCAGC CACTGCACCG ACTTCATCTG GGGTATCGGA 180

AAGCAGGGCC TGCAATGTCA AGTCTGCAGC TTTGTGGTTC ATCGACGATG CCACGAATTT 240

GTGACCTTCG AGTGTCCAGG CGCTGGGAAG GGCCCCCAGA CGGACGACCC CCGGAACAAA 300

CACAAGTTCC GCCTGCATAG CTACAGCAGC CCCACCTTCT GCGACCACTG TGGCTCCCTC 360

CTCTACGGGC TTGTGCACCA GGGCATGAAA TGCTCCTGCT GCGAGATGAA CGTGCACCGG 420

CGCTGTGTGC GTAGCGTGCC CTCCCTGTGC GGTGTGGACC ACACCGAGCG CCGCGGGCGC 480

CTGCAGCTGG AGATCCGGGC TCCCACAGCA GATGAGATCC ACGTAACTGT TGGCGAGGCC 540

CGTAACCTAA TTCCTATGGA CCCCAATGGT CTCTCTGATC CCTATGTGAA ACTGAAGCTC 600

ATCCCAGACC CTCGGAACCT GACGAAACAG AAGACCCGAA CGGTGAAAGC CACGCTAAAC 660

CCTGTGTGGA ATGAGACCTT TGTGTTCAAC CTGAAGCCAG GGGATGTGGA GCGCCGGCTC 720

AGCGTGGAGG TGTGGGACTG GGACCGGACC TCCCGCAACG ACTTCATGGG GGCCATGTCC 780

TTTGGCGTCT CGGAGCTGCT CAAGGCGCCC GTGGATGGCT GGTACAAGTT ACTGAACCAG 840

GAGGAGGGCG AGTATTACAA TGTGCCGGTG GCCGATGCTG ACAACTGCAG CCTCCTCCAG 900

AAGTTTGAGG CTTGTAACTA CCCCCTGGAA TTGTATGAGC GGGTGCGGAT GGGCCCCTCT 960

TCCTCTCCCA TCCCCTCCCC TTCCCCTAGT CCCACCGACC CCAAGCGCTG CTTCTTCGGG 1020

GCGAGTCCAG GACGCCTGCA CATCTCCGAC TTCAGCTTCC TCATGGTTCT AGGAAAAGGC 1080

AGTTTTGGGA AGGTGATGCT GGCCGAGCGC AGGGGCTCTG ATGAGCTCTA CGCCATCAAG 1140

ATCTTGAAAA AGGACGTGAT CGTCCAGGAC GACGATGTGG ACTGCACGCT GGTGGAGAAA 1200

CGTGTGCTGG CGCTGGGGGG CCGGGGTCCT GGCGGCCGGC CCCACTTCCT CACCCAGCTC 1260

CACTCCACCT TCCAGACCCC GGACCGCCTG TATTTCGTGA TGGAGTACGT CACCGGGGGA 1320

GACTTGATGT ACCACATTCA ACAGCTGGGC AAGTTTAAGG AGCCCCATGC AGCGTTCTAC 1380

GCGGCAGAAA TCGCTATCGG CCTCTTCTTC CTTCACAATC AGGGCATCAT CTACAGGGAC 1440

CTGAAGCTGG ACAATGTGAT GCTGGATGCT GAGGGACACA TCAAGATCAC TGACTTTGGC 1500

ATGTGTAAGG AGAACGTCTT CCCCGGGACG ACAACCCGCA CCTTCTGCGG GACCCCGGAC 1560

TACATAGCCC CGGAGATCAT TGCCTACCAG CCCTATGGGA AGTCTGTCGA TTGGTGGTCC 1620

TTTGGAGTTC TGCTGTATGA GATGTTGGCA GGACAGCCTC CCTTCGATGG GGAGGACGAG 1680

GAGGAGCTGT TTCAGGCCAT CATGGAACAA ACTGTCACCT ACCCCAAGTC GCTTTCCCGG 1740

GAAGCCGTGG CCATCTGCAA GGGGTTCCTG ACCAAGCACC CAGGGAAGCG CCTGGGCTCA 1800

GGGCCTGATG GGGAACCTAC CATCCGTGCA CATGGCTTTT TCCGCTGGAT TGACTGGGAG 1860

CGGCTGGAAC GATTGGAGAT CCCGCCTCCT TTCAGACCCC GCCCGTGTGG CCGCAGCGGC 1920

GAGAACTTTG ACAAGTTCTT CACGCGGGCG GCGCCAGCGC TGACCCCTCC AGACCGCCTA 1980

GTCCTGGCCA GCATCGACCA GGCCGATTTC CAGGGCTTCA CCTACGTGAA CCCCGACTTC 2040

GTGCACCCGG ATGCCCGCAG CCCCACCAGC CCAGTGCCTG TGCCCGTCAT GTAA 2094

Domain Profile

S: 1     fellkvlGkGsfgkvllaelkktdelyaikvlkkdvvlqdddveltlvekrvlal.....  55

         f++l+vlGkGsfgkv+lae++++delyaik+lkkdv++qdddv++tlvekrvlal

Q: 351   FSFLMVLGKGSFGKVMLAERRGSDELYAIKILKKDVIVQDDDVDCTLVEKRVLALggrgp  410

         89*****************************************************66666

S: 56    askkpflvqlfscfqtedrlffvleyvnGGdlmfhiqkarklkeerarfyaaeiilalkf  115

           +++fl+ql+s+fqt drl+fv+eyv+GGdlm+hiq+ +k+ke++a+fyaaei+++l+f

Q: 411   GGRPHFLTQLHSTFQTPDRLYFVMEYVTGGDLMYHIQQLGKFKEPHAAFYAAEIAIGLFF  470

         67889*******************************************************

S: 116   lhekgiiyrdlkldnvlldaeGhikladfGlckeeilegkttstfcGtPdyiaPeilkee  175

         lh++giiyrdlkldnv+ldaeGhik++dfG+cke+++ g+tt+tfcGtPdyiaPei++++

Q: 471   LHNQGIIYRDLKLDNVMLDAEGHIKITDFGMCKENVFPGTTTRTFCGTPDYIAPEIIAYQ  530

         ************************************************************

S: 176   eygksvdwwalGvllyemlagqsPfegededelfesilekevlyPkslskeaveilkgll  235

         +ygksvdww++Gvllyemlagq+Pf+gede+elf++i+e++v+yPksls+eav+i+kg+l

Q: 531   PYGKSVDWWSFGVLLYEMLAGQPPFDGEDEEELFQAIMEQTVTYPKSLSREAVAICKGFL  590

         ************************************************************

S: 236   tkdpekrlGvkeegeedikehaff  259

         tk+p krlG+ ++ge +i++h ff

Q: 591   TKHPGKRLGSGPDGEPTIRAHGFF  614

         ***********************8

Domain Sequence
(FASTA)

FSFLMVLGKG SFGKVMLAER RGSDELYAIK ILKKDVIVQD DDVDCTLVEK RVLALGGRGP 60

GGRPHFLTQL HSTFQTPDRL YFVMEYVTGG DLMYHIQQLG KFKEPHAAFY AAEIAIGLFF 120

LHNQGIIYRD LKLDNVMLDA EGHIKITDFG MCKENVFPGT TTRTFCGTPD YIAPEIIAYQ 180

PYGKSVDWWS FGVLLYEMLA GQPPFDGEDE EELFQAIMEQ TVTYPKSLSR EAVAICKGFL 240

TKHPGKRLGS GPDGEPTIRA HGFF 264

Keyword

3D-structure; Acetylation; ATP-binding; Calcium; Cell junction; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Disease mutation; Kinase; Membrane; Metal-binding; Neurodegeneration; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Serine/threonine-protein kinase; Spinocerebellar ataxia; Synapse; Synaptosome; Transferase; Zinc; Zinc-finger.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Equus caballus"; ?>Felis catus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Nomascus leucogenys"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sus scrofa"; ?>Tursiops truncatus"; ?>

Gene Ontology

GO:0030054	; C:cell junction
GO:0005829	; C:cytosol
GO:0030425	; C:dendrite
GO:0005634	; C:nucleus
GO:0048471	; C:perinuclear region of cytoplasm
GO:0005886	; C:plasma membrane
GO:0097060	; C:synaptic membrane
GO:0005524	; F:ATP binding
GO:0004697	; F:protein kinase C activity
GO:0008270	; F:zinc ion binding
GO:0007202	; P:activation of phospholipase C activity
GO:0008219	; P:cell death
GO:0007635	; P:chemosensory behavior
GO:0007173	; P:epidermal growth factor receptor signaling pathway
GO:0008543	; P:fibroblast growth factor receptor signaling pathway
GO:0060384	; P:innervation
GO:0035556	; P:intracellular signal transduction
GO:0007611	; P:learning or memory
GO:0043524	; P:negative regulation of neuron apoptotic process
GO:0042177	; P:negative regulation of protein catabolic process
GO:0031397	; P:negative regulation of protein ubiquitination
GO:0048011	; P:nerve growth factor receptor signaling pathway
GO:0030168	; P:platelet activation
GO:0032425	; P:positive regulation of mismatch repair
GO:0043278	; P:response to morphine
GO:0048265	; P:response to pain
GO:0007268	; P:synaptic transmission

KEGG

hsa:5582

;

InterPros

IPR000961	; AGC-kinase_C.
IPR000008	; C2_Ca-dep.
IPR008973	; C2_Ca/lipid-bd_dom_CaLB.
IPR020477	; C2_dom.
IPR018029	; C2_membr_targeting.
IPR020454	; DAG/PE-bd.
IPR011009	; Kinase-like_dom.
IPR017892	; Pkinase_C.
IPR002219	; Prot_Kinase_C-like_PE/DAG-bd.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR014375	; Protein_kinase_C_a/b/g.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00130	; C1_1; 2.
PF00168	; C2; 1.
PF00069	; Pkinase; 1.
PF00433	; Pkinase_C; 1.

SMARTs

SM00109	; C1; 2.
SM00239	; C2; 1.
SM00133	; S_TK_X; 1.
SM00220	; S_TKc; 1.

Prosites

PS51285	; AGC_KINASE_CTER; 1.
PS50004	; C2; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS00479	; ZF_DAG_PE_1; 2.
PS50081	; ZF_DAG_PE_2; 2.

Prints

PR00360	; C2DOMAIN.
PR00008	; DAGPEDOMAIN.

Created Date

20-Feb-2013