EKS-HOS-00159

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00159

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
AGC/NDR	507.2	6.5E-152	705	1010	306

Status

Reviewed

Ensembl Protein

ENSP00000253339

UniProt Accession

O95835; Q6PKD0;

Protein Name

Serine/threonine-protein kinase LATS1

Protein Synonyms/Alias

Large tumor suppressor homolog 1; WARTS protein kinase; h-warts;

Gene Name

LATS1

Gene Synonyms/Alias

LATS1; WARTS;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000131023	ENSP00000444535	ENST00000542720
ENSG00000131023	ENSP00000444678	ENST00000392273
ENSG00000131023	ENSP00000253339	ENST00000253339
ENSG00000131023	ENSP00000403815	ENST00000441107
ENSG00000131023	ENSP00000441265	ENST00000458696
ENSG00000131023	ENSP00000437550	ENST00000543571

Organism

Homo sapiens

Functional Description

Negative regulator of YAP1 in the Hippo signalingpathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function.

Protein Length

1130

Protein Sequence
(FASTA)

MKRSEKPEGY RQMRPKTFPA SNYTVSSRQM LQEIRESLRN LSKPSDAAKA EHNMSKMSTE 60

DPRQVRNPPK FGTHHKALQE IRNSLLPFAN ETNSSRSTSE VNPQMLQDLQ AAGFDEDMVI 120

QALQKTNNRS IEAAIEFISK MSYQDPRREQ MAAAAARPIN ASMKPGNVQQ SVNRKQSWKG 180

SKESLVPQRH GPPLGESVAY HSESPNSQTD VGRPLSGSGI SAFVQAHPSN GQRVNPPPPP 240

QVRSVTPPPP PRGQTPPPRG TTPPPPSWEP NSQTKRYSGN MEYVISRISP VPPGAWQEGY 300

PPPPLNTSPM NPPNQGQRGI SSVPVGRQPI IMQSSSKFNF PSGRPGMQNG TGQTDFMIHQ 360

NVVPAGTVNR QPPPPYPLTA ANGQSPSALQ TGGSAAPSSY TNGSIPQSMM VPNRNSHNME 420

LYNISVPGLQ TNWPQSSSAP AQSSPSSGHE IPTWQPNIPV RSNSFNNPLG NRASHSANSQ 480

PSATTVTAIT PAPIQQPVKS MRVLKPELQT ALAPTHPSWI PQPIQTVQPS PFPEGTASNV 540

TVMPPVAEAP NYQGPPPPYP KHLLHQNPSV PPYESISKPS KEDQPSLPKE DESEKSYENV 600

DSGDKEKKQI TTSPITVRKN KKDEERRESR IQSYSPQAFK FFMEQHVENV LKSHQQRLHR 660

KKQLENEMMR VGLSQDAQDQ MRKMLCQKES NYIRLKRAKM DKSMFVKIKT LGIGAFGEVC 720

LARKVDTKAL YATKTLRKKD VLLRNQVAHV KAERDILAEA DNEWVVRLYY SFQDKDNLYF 780

VMDYIPGGDM MSLLIRMGIF PESLARFYIA ELTCAVESVH KMGFIHRDIK PDNILIDRDG 840

HIKLTDFGLC TGFRWTHDSK YYQSGDHPRQ DSMDFSNEWG DPSSCRCGDR LKPLERRAAR 900

QHQRCLAHSL VGTPNYIAPE VLLRTGYTQL CDWWSVGVIL FEMLVGQPPF LAQTPLETQM 960

KVINWQTSLH IPPQAKLSPE ASDLIIKLCR GPEDRLGKNG ADEIKAHPFF KTIDFSSDLR 1020

QQSASYIPKI THPTDTSNFD PVDPDKLWSD DNEEENVNDT LNGWYKNGKH PEHAFYEFTF 1080

RRFFDDNGYP YNYPKPIEYE YINSQGSEQQ SDEDDQNTGS EIKNRDLVYV 1130

Nucleotide Sequence
(FASTA)

ATGAAGAGGA GTGAAAAGCC AGAAGGATAT AGACAAATGA GGCCTAAGAC CTTTCCTGCC 60

AGTAACTATA CTGTCAGTAG CCGGCAAATG TTACAAGAAA TTCGGGAATC CCTTAGGAAT 120

TTATCTAAAC CATCTGATGC TGCTAAGGCT GAGCATAACA TGAGTAAAAT GTCAACCGAA 180

GATCCTCGAC AAGTCAGAAA TCCACCCAAA TTTGGGACGC ATCATAAAGC CTTGCAGGAA 240

ATTCGAAACT CTCTGCTTCC ATTTGCAAAT GAAACAAATT CTTCTCGGAG TACTTCAGAA 300

GTTAATCCAC AAATGCTTCA AGACTTGCAA GCTGCTGGAT TTGATGAGGA TATGGTTATA 360

CAAGCTCTTC AGAAAACTAA CAACAGAAGT ATAGAAGCAG CAATTGAATT CATTAGTAAA 420

ATGAGTTACC AAGATCCTCG ACGAGAGCAG ATGGCTGCAG CAGCTGCCAG ACCTATTAAT 480

GCCAGCATGA AACCAGGGAA TGTGCAGCAA TCAGTTAACC GCAAACAGAG CTGGAAAGGT 540

TCTAAAGAAT CCTTAGTTCC TCAGAGGCAT GGCCCGCCAC TAGGAGAAAG TGTGGCCTAT 600

CATTCTGAGA GTCCCAACTC ACAGACAGAT GTAGGAAGAC CTTTGTCTGG ATCTGGTATA 660

TCAGCATTTG TTCAAGCTCA CCCTAGCAAC GGACAGAGAG TGAACCCCCC ACCACCACCT 720

CAAGTAAGGA GTGTTACTCC TCCACCACCT CCAAGAGGCC AGACTCCCCC TCCAAGAGGT 780

ACAACTCCAC CTCCCCCTTC ATGGGAACCA AACTCTCAAA CAAAGCGCTA TTCTGGAAAC 840

ATGGAATACG TAATCTCCCG AATCTCTCCT GTCCCACCTG GGGCATGGCA AGAGGGCTAT 900

CCTCCACCAC CTCTCAACAC TTCCCCCATG AATCCTCCTA ATCAAGGACA GAGAGGCATT 960

AGTTCTGTTC CTGTTGGCAG ACAACCAATC ATCATGCAGA GTTCTAGCAA ATTTAACTTT 1020

CCATCAGGGA GACCTGGAAT GCAGAATGGT ACTGGACAAA CTGATTTCAT GATACACCAA 1080

AATGTTGTCC CTGCTGGCAC TGTGAATCGG CAGCCACCAC CTCCATATCC TCTGACAGCA 1140

GCTAATGGAC AAAGCCCTTC TGCTTTACAA ACAGGGGGAT CTGCTGCTCC TTCGTCATAT 1200

ACAAATGGAA GTATTCCTCA GTCTATGATG GTGCCAAACA GAAATAGTCA TAACATGGAA 1260

CTATATAACA TTAGTGTACC TGGACTGCAA ACAAATTGGC CTCAGTCATC TTCTGCTCCA 1320

GCCCAGTCAT CCCCGAGCAG TGGGCATGAA ATCCCTACAT GGCAACCTAA CATACCAGTG 1380

AGGTCAAATT CTTTTAATAA CCCATTAGGA AATAGAGCAA GTCACTCTGC TAATTCTCAG 1440

CCTTCTGCTA CAACAGTCAC TGCAATTACA CCAGCTCCTA TTCAACAGCC TGTGAAAAGT 1500

ATGCGTGTAT TAAAACCAGA GCTACAGACT GCTTTAGCAC CTACACACCC TTCTTGGATA 1560

CCACAGCCAA TTCAAACTGT TCAACCCAGT CCTTTTCCTG AGGGAACCGC TTCAAATGTG 1620

ACTGTGATGC CACCTGTTGC TGAAGCTCCA AACTATCAAG GACCACCACC ACCCTACCCA 1680

AAACATCTGC TGCACCAAAA CCCATCTGTT CCTCCATACG AGTCAATCAG TAAGCCTAGC 1740

AAAGAGGATC AGCCAAGCTT GCCCAAGGAA GATGAGAGTG AAAAGAGTTA TGAAAATGTT 1800

GATAGTGGGG ATAAAGAAAA GAAACAGATT ACAACTTCAC CTATTACTGT TAGGAAAAAC 1860

AAGAAAGATG AAGAGCGAAG GGAATCTCGT ATTCAAAGTT ATTCTCCTCA AGCATTTAAA 1920

TTCTTTATGG AGCAACATGT AGAAAATGTA CTCAAATCTC ATCAGCAGCG TCTACATCGT 1980

AAAAAACAAT TAGAGAATGA AATGATGCGG GTTGGATTAT CTCAAGATGC CCAGGATCAA 2040

ATGAGAAAGA TGCTTTGCCA AAAAGAATCT AATTACATCC GTCTTAAAAG GGCTAAAATG 2100

GACAAGTCTA TGTTTGTGAA GATAAAGACA CTAGGAATAG GAGCATTTGG TGAAGTCTGT 2160

CTAGCAAGAA AAGTAGATAC TAAGGCTTTG TATGCAACAA AAACTCTTCG AAAGAAAGAT 2220

GTTCTTCTTC GAAATCAAGT CGCTCATGTT AAGGCTGAGA GAGATATCCT GGCTGAAGCT 2280

GACAATGAAT GGGTAGTTCG TCTATATTAT TCATTCCAAG ATAAGGACAA TTTATACTTT 2340

GTAATGGACT ACATTCCTGG GGGTGATATG ATGAGCCTAT TAATTAGAAT GGGCATCTTT 2400

CCAGAAAGTC TGGCACGATT CTACATAGCA GAACTTACCT GTGCAGTTGA AAGTGTTCAT 2460

AAAATGGGTT TTATTCATAG AGATATTAAA CCTGATAATA TTTTGATTGA TCGTGATGGT 2520

CATATTAAAT TGACTGACTT TGGCCTCTGC ACTGGCTTCA GATGGACACA CGATTCTAAG 2580

TACTATCAGA GTGGTGACCA TCCACGGCAA GATAGCATGG ATTTCAGTAA TGAATGGGGG 2640

GATCCCTCAA GCTGTCGATG TGGAGACAGA CTGAAGCCAT TAGAGCGGAG AGCTGCACGC 2700

CAGCACCAGC GATGTCTAGC ACATTCTTTG GTTGGGACTC CCAATTATAT TGCACCTGAA 2760

GTGTTGCTAC GAACAGGATA CACACAGTTG TGTGATTGGT GGAGTGTTGG TGTTATTCTT 2820

TTTGAAATGT TGGTGGGACA ACCTCCTTTC TTGGCACAAA CACCATTAGA AACACAAATG 2880

AAGGTTATCA ACTGGCAAAC ATCTCTTCAC ATTCCACCAC AAGCTAAACT CAGTCCTGAA 2940

GCTTCTGATC TTATTATTAA ACTTTGCCGA GGACCCGAAG ATCGCTTAGG CAAGAATGGT 3000

GCTGATGAAA TAAAAGCTCA TCCATTTTTT AAAACAATTG ACTTCTCCAG TGACCTGAGA 3060

CAGCAGTCTG CTTCATACAT TCCTAAAATC ACACACCCAA CAGATACATC AAATTTTGAT 3120

CCTGTTGATC CTGATAAATT ATGGAGTGAT GATAACGAGG AAGAAAATGT AAATGACACT 3180

CTCAATGGAT GGTATAAAAA TGGAAAGCAT CCTGAACATG CATTCTATGA ATTTACCTTC 3240

CGAAGGTTTT TTGATGACAA TGGCTACCCA TATAATTATC CGAAGCCTAT TGAATATGAA 3300

TACATTAATT CACAAGGCTC AGAGCAGCAG TCGGATGAAG ATGATCAAAA CACAGGCTCA 3360

GAGATTAAAA ATCGCGATCT AGTATATGTT TAA 3393

Domain Profile

S: 1     feklkvigkGafGevrlvrkkdtkevyalkilrkkdllkknqvahvkaerdilaeadsew  60

         f k+k++g+GafGev+l+rk dtk++ya+k+lrkkd+l +nqvahvkaerdilaead+ew

Q: 705   FVKIKTLGIGAFGEVCLARKVDTKALYATKTLRKKDVLLRNQVAHVKAERDILAEADNEW  764

         889*********************************************************

S: 61    vvklyysfqdkenlylvmeylPGGdlmtllikkeilkeelarfyiaelvlavesvhklGf  120

         vv+lyysfqdk+nly+vm+y+PGGd+m+lli+++i++e+larfyiael++avesvhk+Gf

Q: 765   VVRLYYSFQDKDNLYFVMDYIPGGDMMSLLIRMGIFPESLARFYIAELTCAVESVHKMGF  824

         ************************************************************

S: 121   ihrdlkPdnllidakGhikltdfGlctGlkkthrseyykelekakeddlease.......  173

         ihrd+kPdn+lid++GhikltdfGlctG+++th+s+yy++ ++ ++d++++s+

Q: 825   IHRDIKPDNILIDRDGHIKLTDFGLCTGFRWTHDSKYYQSGDHPRQDSMDFSNewgdpss  884

         ***************************************************999******

S: 174   .....elkvlerrkkkknrrllayslvGtpdyiapevlekkgynklcdwWslGvilyeml  228

              +lk+lerr++++++r+la+slvGtp+yiapevl+++gy++lcdwWs+Gvil+eml

Q: 885   crcgdRLKPLERRAARQHQRCLAHSLVGTPNYIAPEVLLRTGYTQLCDWWSVGVILFEML  944

         ************************************************************

S: 229   vGypPflaetpqetykkvinwketlkfpeevelseeakdlierliadaekrlgkkGvdei  288

         vG+pPfla+tp et++kvinw+++l++p++++ls+ea+dli +l+  +e+rlgk+G+dei

Q: 945   VGQPPFLAQTPLETQMKVINWQTSLHIPPQAKLSPEASDLIIKLCRGPEDRLGKNGADEI  1004

         ************************************************************

S: 289   kkhpff  294

         k+hpff

Q: 1005  KAHPFF  1010

         *****9

Domain Sequence
(FASTA)

FVKIKTLGIG AFGEVCLARK VDTKALYATK TLRKKDVLLR NQVAHVKAER DILAEADNEW 60

VVRLYYSFQD KDNLYFVMDY IPGGDMMSLL IRMGIFPESL ARFYIAELTC AVESVHKMGF 120

IHRDIKPDNI LIDRDGHIKL TDFGLCTGFR WTHDSKYYQS GDHPRQDSMD FSNEWGDPSS 180

CRCGDRLKPL ERRAARQHQR CLAHSLVGTP NYIAPEVLLR TGYTQLCDWW SVGVILFEML 240

VGQPPFLAQT PLETQMKVIN WQTSLHIPPQ AKLSPEASDL IIKLCRGPED RLGKNGADEI 300

KAHPFF 306

Keyword

Alternative splicing; ATP-binding; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Magnesium; Metal-binding; Mitosis; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase; Tumor suppressor.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005829	; C:cytosol
GO:0005815	; C:microtubule organizing center
GO:0000922	; C:spindle pole
GO:0005524	; F:ATP binding
GO:0000287	; F:magnesium ion binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0051301	; P:cell division
GO:0051220	; P:cytoplasmic sequestering of protein
GO:0000086	; P:G2/M transition of mitotic cell cycle
GO:0035329	; P:hippo signaling cascade
GO:0009755	; P:hormone-mediated signaling pathway
GO:0030216	; P:keratinocyte differentiation
GO:0007067	; P:mitosis
GO:0090090	; P:negative regulation of canonical Wnt receptor signaling pathway
GO:0045736	; P:negative regulation of cyclin-dependent protein kinase activity
GO:0033138	; P:positive regulation of peptidyl-serine phosphorylation
GO:0030833	; P:regulation of actin filament polymerization
GO:0000819	; P:sister chromatid segregation

KEGG

hsa:9113

;

InterPros

IPR000961	; AGC-kinase_C.
IPR011009	; Kinase-like_dom.
IPR017892	; Pkinase_C.
IPR000719	; Prot_kinase_cat_dom.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.
IPR009060	; UBA-like.
IPR000449	; UBA/transl_elong_EF1B_N.
IPR015940	; UBA/transl_elong_EF1B_N_euk.

Pfam

PF00069	; Pkinase; 2.
PF00433	; Pkinase_C; 1.
PF00627	; UBA; 1.

SMARTs

SM00133	; S_TK_X; 1.
SM00220	; S_TKc; 1.

Prosites

PS51285	; AGC_KINASE_CTER; 1.
PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS50030	; UBA; 1.

Prints

Created Date

20-Feb-2013