EKS-HOS-00073

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00073

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CMGC/CDK	338.9	1.1E-100	26	335	310

Status

Reviewed

Ensembl Protein

ENSP00000370938

UniProt Accession

P49336; Q5VUF3; Q6ISB5;

Protein Name

Cyclin-dependent kinase 8

Protein Synonyms/Alias

Cell division protein kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; Protein kinase K35;

Gene Name

CDK8

Gene Synonyms/Alias

CDK8;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000132964	ENSP00000370938	ENST00000381527
ENSG00000132964	ENSP00000437696	ENST00000536792

Organism

Homo sapiens

Functional Description

Component of the Mediator complex, a coactivatorinvolved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.

Protein Length

464

Protein Sequence
(FASTA)

MDYDFKVKLS SERERVEDLF EYEGCKVGRG TYGHVYKAKR KDGKDDKDYA LKQIEGTGIS 60

MSACREIALL RELKHPNVIS LQKVFLSHAD RKVWLLFDYA EHDLWHIIKF HRASKANKKP 120

VQLPRGMVKS LLYQILDGIH YLHANWVLHR DLKPANILVM GEGPERGRVK IADMGFARLF 180

NSPLKPLADL DPVVVTFWYR APELLLGARH YTKAIDIWAI GCIFAELLTS EPIFHCRQED 240

IKTSNPYHHD QLDRIFNVMG FPADKDWEDI KKMPEHSTLM KDFRRNTYTN CSLIKYMEKH 300

KVKPDSKAFH LLQKLLTMDP IKRITSEQAM QDPYFLEDPL PTSDVFAGCQ IPYPKREFLT 360

EEEPDDKGDK KNQQQQQGNN HTNGTGHPGN QDSSHTQGPP LKKVRVVPPT TTSGGLIMTS 420

DYQRSNPHAA YPNPGPSTSQ PQSSMGYSAT SQQPPQYSHQ THRY 464

Nucleotide Sequence
(FASTA)

ATGGACTATG ACTTTAAAGT GAAGCTGAGC AGCGAGCGGG AGCGGGTCGA GGACCTGTTT 60

GAATACGAGG GCTGCAAAGT TGGCCGAGGC ACTTATGGTC ACGTCTACAA AGCCAAGAGG 120

AAAGATGGGA AGGATGATAA AGACTATGCT TTAAAACAAA TAGAAGGAAC TGGGATCTCT 180

ATGTCGGCAT GTAGAGAAAT AGCATTACTT CGAGAGCTTA AGCATCCAAA CGTCATTTCT 240

CTTCAAAAGG TGTTTCTGTC TCATGCTGAT AGGAAGGTGT GGCTTCTGTT TGACTATGCT 300

GAACATGACC TCTGGCATAT AATCAAGTTT CACAGAGCTT CTAAAGCAAA CAAGAAGCCA 360

GTTCAGTTAC CTCGGGGAAT GGTGAAGTCA CTATTATATC AGATCCTAGA TGGTATTCAC 420

TACCTGCATG CTAACTGGGT GTTGCACAGA GATTTGAAAC CTGCTAATAT TTTAGTTATG 480

GGTGAAGGTC CTGAGCGAGG AAGAGTAAAA ATTGCTGACA TGGGCTTTGC CCGATTATTT 540

AATTCACCTT TGAAGCCTTT AGCAGATTTG GATCCAGTGG TTGTTACATT CTGGTACCGA 600

GCCCCTGAAC TACTTCTTGG AGCAAGGCAT TATACCAAAG CTATTGATAT TTGGGCTATA 660

GGGTGTATAT TTGCAGAACT ACTAACGTCA GAACCAATAT TTCACTGTCG ACAAGAGGAC 720

ATCAAAACTA GTAATCCTTA TCACCATGAC CAGCTGGACA GAATATTCAA TGTAATGGGA 780

TTTCCTGCAG ATAAAGATTG GGAAGATATA AAAAAGATGC CTGAACATTC AACATTAATG 840

AAAGATTTCA GAAGAAATAC GTATACCAAC TGCAGCCTTA TCAAGTATAT GGAAAAACAT 900

AAAGTTAAAC CAGATAGTAA AGCATTCCAC TTGCTTCAGA AGCTGCTTAC CATGGACCCA 960

ATAAAGCGAA TTACCTCAGA ACAGGCTATG CAGGACCCCT ATTTCTTAGA AGACCCACTT 1020

CCTACATCAG ACGTTTTTGC CGGTTGTCAA ATCCCTTACC CAAAACGAGA ATTTTTAACG 1080

GAAGAAGAAC CTGATGACAA AGGAGACAAA AAGAACCAGC AGCAGCAGCA GGGCAATAAC 1140

CACACTAATG GAACTGGCCA CCCAGGGAAT CAAGACAGCA GTCACACACA GGGACCCCCG 1200

TTGAAGAAAG TGAGAGTTGT TCCTCCTACC ACTACCTCAG GTGGACTTAT CATGACCTCA 1260

GACTATCAGC GTTCCAATCC ACATGCTGCC TATCCCAACC CTGGACCAAG CACATCACAG 1320

CCGCAGAGCA GCATGGGATA CTCAGCTACC TCCCAGCAGC CTCCACAGTA CTCACATCAG 1380

ACACATCGGT ACTGA 1395

Domain Profile

S: 6     kigeGtygkvykakdke..tgevvAlKkirlekekegvpitalrEisllkelkhknivkL  63

         k+g+Gtyg+vykak+k+  +++ +AlK+i    e +g++++a+rEi+ll+elkh+n+++L

Q: 26    KVGRGTYGHVYKAKRKDgkDDKDYALKQI----EGTGISMSACREIALLRELKHPNVISL  81

         79************986447778******....999************************

S: 64    levva.kdkkklyLvfefleqdLkkllkkkkkk.......klsleevkslllqlleglay  115

         ++v+  ++++k++L+f+++e+dL++++k ++++       +l++ +vksll+q+l+g++y

Q: 82    QKVFLsHADRKVWLLFDYAEHDLWHIIKFHRASkankkpvQLPRGMVKSLLYQILDGIHY  141

         ****94555*********************9999999999********************

S: 116   lHsnkilHRDlKpqNlLiske....gklklaDfGlarafssplk...tytkevvTlwYra  168

         lH+n++lHRDlKp+N+L++ e    g++k+aD+G+ar f+splk   +++++vvT+wYra

Q: 142   LHANWVLHRDLKPANILVMGEgperGRVKIADMGFARLFNSPLKplaDLDPVVVTFWYRA  201

         *******************9988889*************99999665699**********

S: 169   PelLlgakeYstavDiWsvgcilaelltrkplfqgkse.........idqlerifkllgt  219

         PelLlga++Y++a+DiW++gci+aellt++p+f++++e         +dql+rif+++g

Q: 202   PELLLGARHYTKAIDIWAIGCIFAELLTSEPIFHCRQEdiktsnpyhHDQLDRIFNVMGF  261

         ************************************************************

S: 220   psekvwpevsklpeyk...ksfpkkkkk..klkekvkklk....ekaldllekllaldpk  270

         p +k+w++++k+pe++   k+f++++++  +l ++++k k    +ka++ll+kll++dp

Q: 262   PADKDWEDIKKMPEHStlmKDFRRNTYTncSLIKYMEKHKvkpdSKAFHLLQKLLTMDPI  321

         ***************96644777777764455666644333354679*************

S: 271   kRisakealqhkyf  284

         kRi++++a+q++yf

Q: 322   KRITSEQAMQDPYF  335

         *************9

Domain Sequence
(FASTA)

KVGRGTYGHV YKAKRKDGKD DKDYALKQIE GTGISMSACR EIALLRELKH PNVISLQKVF 60

LSHADRKVWL LFDYAEHDLW HIIKFHRASK ANKKPVQLPR GMVKSLLYQI LDGIHYLHAN 120

WVLHRDLKPA NILVMGEGPE RGRVKIADMG FARLFNSPLK PLADLDPVVV TFWYRAPELL 180

LGARHYTKAI DIWAIGCIFA ELLTSEPIFH CRQEDIKTSN PYHHDQLDRI FNVMGFPADK 240

DWEDIKKMPE HSTLMKDFRR NTYTNCSLIK YMEKHKVKPD SKAFHLLQKL LTMDPIKRIT 300

SEQAMQDPYF 310

Keyword

3D-structure; Activator; Alternative splicing; ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repressor; Serine/threonine-protein kinase; Transcription; Transcription regulation; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>

Gene Ontology

GO:0016592	; C:mediator complex
GO:0005524	; F:ATP binding
GO:0004693	; F:cyclin-dependent protein kinase activity
GO:0004672	; F:protein kinase activity
GO:0008353	; F:RNA polymerase II carboxy-terminal domain kinase activity
GO:0007049	; P:cell cycle
GO:0007219	; P:Notch signaling pathway
GO:0045944	; P:positive regulation of transcription from RNA polymerase II promoter
GO:0006367	; P:transcription initiation from RNA polymerase II promoter
GO:0007179	; P:transforming growth factor beta receptor signaling pathway

KEGG

hsa:1024

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013