EKS-HOS-00294

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00294

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/IRE	445.1	4.2E-133	468	729	262

Status

Reviewed

Ensembl Protein

ENSP00000413812

UniProt Accession

Q76MJ5; Q6ZNC0;

Protein Name

Endoribonuclease

Protein Synonyms/Alias

Endoplasmic reticulum-to-nucleus signaling 2; Inositol-requiring protein 2; hIRE2p; Ire1-beta; IRE1b;

Gene Name

ERN2

Gene Synonyms/Alias

ERN2; IRE2;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000134398	ENSP00000457796	ENST00000569903
ENSG00000134398	ENSP00000413812	ENST00000457008
ENSG00000134398	ENSP00000457361	ENST00000562562
ENSG00000134398	ENSP00000256797	ENST00000256797
ENSG00000134398	ENSP00000456866	ENST00000562458

Organism

Homo sapiens

Functional Description

Induces translational repression through 28S ribosomalRNA cleavage in response to ER stress. Pro-apoptotic. Appears to play no role in the unfolded-protein response, unlike closely related proteins.

Protein Length

874

Protein Sequence
(FASTA)

MASAVRGSRP WPRLGLQLQF AALLLGTLSP QVHTLRPENL LLVSTLDGSL HALSKQTGDL 60

KWTLRDDPVI EGPMYVTEMA FLSDPADGSL YILGTQKQQG LMKLPFTIPE LVHASPCRSS 120

DGVFYTGRKQ DAWFVVDPES GETQMTLTTE GPSTPRLYIG RTQYTVTMHD PRAPALRWNT 180

TYRRYSAPPM DGSPGKYMSH LASCGMGLLL TVDPGSGTVL WTQDLGVPVM GVYTWHQDGL 240

RQLPHLTLAR DTLHFLALRW GHIRLPASGP RDTATLFSTL DTQLLMTLYV GKDETGFYVS 300

KALVHTGVAL VPRGLTLAPA DGPTTDEVTL QVSGEREGSP STAVRYPSGS VALPSQWLLI 360

GHHELPPVLH TTMLRVHPTL GSGTAETRPP ENTQAPAFFL EQQPQVVEKQ QETPLAPADF 420

AHISQDAQSL HSGASRRSQK RLQSPSKQAQ PLDDPEAEQL TVVGKISFNP KDVLGRGAGG 480

TFVFRGQFEG RAVAVKRLLR ECFGLVRREV QLLQESDRHP NVLRYFCTER GPQFHYIALE 540

LCRASLQEYV ENPDLDRGGL EPEVVLQQLM SGLAHLHSLH IVHRDLKPGN ILITGPDSQG 600

LGRVVLSDFG LCKKLPAGRC SFSLHSGIPG TEGWMAPELL QLLPPDSPTS AVDIFSAGCV 660

FYYVLSGGSH PFGDSLYRQA NILTGAPCLA HLEEEVHDKV VARDLVGAML SPLPQPRPSA 720

PQVLAHPFFW SRAKQLQFFQ DVSDWLEKES EQEPLVRALE AGGCAVVRDN WHEHISMPLQ 780

TDLRKFRSYK GTSVRDLLRA VRNKKHHYRE LPVEVRQALG QVPDGFVQYF TNRFPRLLLH 840

THRAMRSCAS ESLFLPYYPP DSEARRPCPG ATGR 874

Nucleotide Sequence
(FASTA)

ATGGCGAGTG CGGTCAGGGG GTCGAGGCCG TGGCCCCGGC TGGGGCTCCA GCTCCAGTTC 60

GCGGCGCTGC TGCTCGGGAC GCTGAGTCCA CAGGTTCATA CTCTCAGGCC AGAGAACCTC 120

CTGCTGGTGT CCACCTTGGA TGGAAGTCTC CACGCACTAA GCAAGCAGAC AGGGGACCTG 180

AAGTGGACTC TGAGGGATGA TCCCGTCATC GAAGGACCAA TGTACGTCAC AGAAATGGCC 240

TTTCTCTCTG ACCCAGCAGA TGGCAGCCTG TACATCTTGG GGACCCAAAA ACAACAGGGA 300

TTAATGAAAC TGCCATTCAC CATCCCTGAG CTGGTTCATG CCTCTCCCTG CCGCAGCTCT 360

GATGGGGTCT TCTACACAGG CCGGAAGCAG GATGCCTGGT TTGTGGTGGA CCCTGAGTCA 420

GGGGAGACCC AGATGACACT GACCACAGAG GGTCCCTCCA CCCCCCGCCT CTACATTGGC 480

CGAACACAGT ATACGGTCAC CATGCATGAC CCAAGAGCCC CAGCCCTGCG CTGGAACACC 540

ACCTACCGCC GCTACTCAGC GCCCCCCATG GATGGCTCAC CTGGGAAATA CATGAGCCAC 600

CTGGCGTCCT GCGGGATGGG CCTGCTGCTC ACTGTGGACC CAGGAAGCGG GACGGTGCTG 660

TGGACACAGG ACCTGGGCGT GCCTGTGATG GGCGTCTACA CCTGGCACCA GGACGGCCTG 720

CGCCAGCTGC CGCATCTCAC GCTGGCTCGA GACACTCTGC ATTTCCTCGC CCTCCGCTGG 780

GGCCACATCC GACTGCCTGC CTCAGGCCCC CGGGACACAG CCACCCTCTT CTCTACCTTG 840

GACACCCAGC TGCTAATGAC GCTGTATGTG GGGAAGGATG AAACTGGCTT CTATGTCTCT 900

AAAGCACTGG TCCACACAGG AGTGGCCCTG GTGCCTCGTG GACTGACCCT GGCCCCCGCA 960

GATGGCCCCA CCACAGATGA GGTGACACTC CAAGTCTCAG GAGAGCGAGA GGGCTCACCC 1020

AGCACTGCTG TTAGATACCC CTCAGGCAGT GTGGCCCTCC CAAGCCAGTG GCTGCTCATT 1080

GGACACCACG AGCTACCCCC AGTCCTGCAC ACCACCATGC TGAGGGTCCA TCCCACCCTG 1140

GGGAGTGGAA CTGCAGAGAC AAGACCTCCA GAGAATACCC AGGCCCCAGC CTTCTTCTTG 1200

GAGCAACAGC CGCAGGTGGT GGAGAAGCAG CAGGAGACCC CCCTGGCACC TGCAGACTTT 1260

GCTCACATCT CCCAGGATGC CCAGTCCCTG CACTCGGGGG CCAGCCGGAG GAGCCAGAAG 1320

AGGCTTCAGA GTCCCTCAAA GCAAGCCCAG CCACTCGACG ACCCTGAAGC TGAGCAACTC 1380

ACCGTAGTGG GGAAGATTTC CTTCAATCCC AAGGACGTGC TGGGCCGCGG GGCAGGCGGG 1440

ACTTTCGTTT TCCGGGGACA GTTTGAGGGA CGGGCAGTGG CTGTCAAGCG GCTCCTCCGC 1500

GAGTGCTTTG GCCTGGTTCG GCGGGAAGTT CAACTGCTGC AGGAGTCTGA CAGGCACCCC 1560

AACGTGCTCC GCTACTTCTG CACCGAGCGG GGACCCCAGT TCCACTACAT TGCCCTGGAG 1620

CTCTGCCGGG CCTCCTTGCA GGAGTACGTA GAAAACCCGG ACCTGGATCG CGGGGGTCTG 1680

GAGCCCGAGG TCGTGCTGCA GCAGCTGATG TCTGGCCTGG CCCACCTGCA CTCTTTACAC 1740

ATAGTGCACC GGGACCTGAA GCCAGGAAAT ATTCTCATCA CCGGGCCTGA CAGCCAGGGC 1800

CTGGGCAGAG TGGTGCTCTC AGACTTCGGC CTCTGCAAGA AGCTGCCTGC TGGCCGCTGT 1860

AGCTTCAGCC TCCACTCCGG CATCCCCGGC ACGGAAGGCT GGATGGCGCC CGAGCTTCTG 1920

CAGCTCCTGC CACCAGACAG TCCTACCAGC GCTGTGGACA TCTTCTCTGC AGGCTGCGTG 1980

TTCTACTACG TGCTTTCTGG TGGCAGCCAC CCCTTTGGAG ACAGTCTTTA TCGCCAGGCA 2040

AACATCCTCA CAGGGGCTCC CTGTCTGGCT CACCTGGAGG AAGAGGTCCA CGACAAGGTG 2100

GTTGCCCGGG ACCTGGTTGG AGCCATGTTG AGCCCACTGC CGCAGCCACG CCCCTCTGCC 2160

CCCCAGGTGC TGGCCCACCC CTTCTTTTGG AGCAGAGCCA AGCAACTCCA GTTCTTCCAG 2220

GACGTCAGTG ACTGGCTGGA GAAGGAGTCC GAGCAGGAGC CCCTGGTGAG GGCACTGGAG 2280

GCGGGAGGCT GCGCAGTGGT CCGGGACAAC TGGCACGAGC ACATCTCCAT GCCGCTGCAG 2340

ACAGATCTGA GAAAGTTCCG GTCCTATAAG GGGACATCAG TGCGAGACCT GCTCCGTGCT 2400

GTGAGGAACA AGAAGCACCA CTACAGGGAG CTCCCAGTTG AGGTGCGACA GGCACTCGGC 2460

CAAGTCCCTG ATGGCTTCGT CCAGTACTTC ACAAACCGCT TCCCACGGCT GCTCCTCCAC 2520

ACGCACCGAG CCATGAGGAG CTGCGCCTCT GAGAGCCTCT TCCTGCCCTA CTACCCGCCA 2580

GACTCAGAGG CCAGGAGGCC ATGCCCTGGG GCCACAGGGA GGTGA 2625

Domain Profile

S: 1     fspkdvlGkGaeGtvvfrGsfegrevavkrllsefvdlakrevkllresdehpnviryfc  60

         f+pkdvlG+Ga+Gt+vfrG+fegr+vavkrll+e++ l++rev+ll+esd+hpnv+ryfc

Q: 468   FNPKDVLGRGAGGTFVFRGQFEGRAVAVKRLLRECFGLVRREVQLLQESDRHPNVLRYFC  527

         799*********************************************************

S: 61    tekdrqfryialelcaaslqdyvedkdlelqkelepisllkqiasGlahlhslkivhrdl  120

         te+++qf+yialelc+aslq+yve++dl+ + +lep  +l+q++sGlahlhsl+ivhrdl

Q: 528   TERGPQFHYIALELCRASLQEYVENPDLD-RGGLEPEVVLQQLMSGLAHLHSLHIVHRDL  586

         *****************************.******************************

S: 121   kpqnilislpdakgklrvlisdfGlckkldvGkssfsrrsgvsGteGwiapelleeltke  180

         kp nili+ pd++g  rv++sdfGlckkl++G++sfs +sg++GteGw+apell+ l+++

Q: 587   KPGNILITGPDSQGLGRVVLSDFGLCKKLPAGRCSFSLHSGIPGTEGWMAPELLQLLPPD  646

         ************************************************************

S: 181   rltsavdifslGcvfyyvlskGkhpfGdslkrqanilsgelsldklkeekdeeviaedli  240

         ++tsavdifs+Gcvfyyvls+G+hpfGdsl+rqanil+g+ +l++l+ee +++v+a+dl+

Q: 647   SPTSAVDIFSAGCVFYYVLSGGSHPFGDSLYRQANILTGAPCLAHLEEEVHDKVVARDLV  706

         ************************************************************

S: 241   skmidkdplkrpsakkvlkhplf  263

          +m++  p+ rpsa +vl+hp+f

Q: 707   GAMLSPLPQPRPSAPQVLAHPFF  729

         **********************9

Domain Sequence
(FASTA)

FNPKDVLGRG AGGTFVFRGQ FEGRAVAVKR LLRECFGLVR REVQLLQESD RHPNVLRYFC 60

TERGPQFHYI ALELCRASLQ EYVENPDLDR GGLEPEVVLQ QLMSGLAHLH SLHIVHRDLK 120

PGNILITGPD SQGLGRVVLS DFGLCKKLPA GRCSFSLHSG IPGTEGWMAP ELLQLLPPDS 180

PTSAVDIFSA GCVFYYVLSG GSHPFGDSLY RQANILTGAP CLAHLEEEVH DKVVARDLVG 240

AMLSPLPQPR PSAPQVLAHP FF 262

Keyword

Apoptosis; ATP-binding; Complete proteome; Endoplasmic reticulum; Hydrolase; Kinase; Magnesium; Membrane; Metal-binding; Multifunctional enzyme; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Signal; Transcription; Transcription regulation; Transferase; Transmembrane; Transmembrane helix.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Echinops telfairi"; ?>Felis catus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Ochotona princeps"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Tetraodon nigroviridis"; ?>Tupaia belangeri"; ?>

Gene Ontology

GO:0005789	; C:endoplasmic reticulum membrane
GO:0016021	; C:integral to membrane
GO:0005524	; F:ATP binding
GO:0004519	; F:endonuclease activity
GO:0016891	; F:endoribonuclease activity, producing 5'-phosphomonoesters
GO:0000287	; F:magnesium ion binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0006915	; P:apoptotic process
GO:0007050	; P:cell cycle arrest
GO:0006917	; P:induction of apoptosis
GO:0006397	; P:mRNA processing
GO:0045892	; P:negative regulation of transcription, DNA-dependent
GO:0006950	; P:response to stress
GO:0016075	; P:rRNA catabolic process
GO:0006351	; P:transcription, DNA-dependent

KEGG

hsa:10595

;

InterPros

IPR010513	; KEN_RNase_activator.
IPR011009	; Kinase-like_dom.
IPR018391	; PQQ_beta_propeller_repeat.
IPR000719	; Prot_kinase_cat_dom.
IPR006567	; PUG-dom.
IPR011047	; Quinonprotein_ADH-like.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069	; Pkinase; 1.
PF06479	; Ribonuc_2-5A; 1.

SMARTs

SM00564	; PQQ; 4.
SM00580	; PUG; 1.

Prosites

PS51392	; KEN; 1.
PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013