EKS-HOS-00407

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00407

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/PDGFR	614.8	2.4E-184	593	950	358

Status

Reviewed

Ensembl Protein

ENSP00000257290

UniProt Accession

P16234; B2RE69; E9PBH0; Q6P4H5; Q96KZ7; Q9UD28;

Protein Name

Platelet-derived growth factor receptor alpha

Protein Synonyms/Alias

PDGF-R-alpha; PDGFR-alpha; Alpha platelet-derived growth factor receptor; Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor 2; PDGFR-2;

Gene Name

PDGFRA

Gene Synonyms/Alias

PDGFRA; PDGFR2, RHEPDGFRA;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000134853	ENSP00000425626	ENST00000512143
ENSG00000134853	ENSP00000425648	ENST00000508170
ENSG00000134853	ENSP00000424218	ENST00000509490
ENSG00000134853	ENSP00000425232	ENST00000512522
ENSG00000134853	ENSP00000425902	ENST00000503856
ENSG00000134853	ENSP00000426472	ENST00000504461
ENSG00000134853	ENSP00000257290	ENST00000257290

Organism

Homo sapiens

Functional Description

Tyrosine-protein kinase that acts as a cell-surfacereceptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.

Protein Length

1089

Protein Sequence
(FASTA)

MGTSHPAFLV LGCLLTGLSL ILCQLSLPSI LPNENEKVVQ LNSSFSLRCF GESEVSWQYP 60

MSEEESSDVE IRNEENNSGL FVTVLEVSSA SAAHTGLYTC YYNHTQTEEN ELEGRHIYIY 120

VPDPDVAFVP LGMTDYLVIV EDDDSAIIPC RTTDPETPVT LHNSEGVVPA SYDSRQGFNG 180

TFTVGPYICE ATVKGKKFQT IPFNVYALKA TSELDLEMEA LKTVYKSGET IVVTCAVFNN 240

EVVDLQWTYP GEVKGKGITM LEEIKVPSIK LVYTLTVPEA TVKDSGDYEC AARQATREVK 300

EMKKVTISVH EKGFIEIKPT FSQLEAVNLH EVKHFVVEVR AYPPPRISWL KNNLTLIENL 360

TEITTDVEKI QEIRYRSKLK LIRAKEEDSG HYTIVAQNED AVKSYTFELL TQVPSSILDL 420

VDDHHGSTGG QTVRCTAEGT PLPDIEWMIC KDIKKCNNET SWTILANNVS NIITEIHSRD 480

RSTVEGRVTF AKVEETIAVR CLAKNLLGAE NRELKLVAPT LRSELTVAAA VLVLLVIVII 540

SLIVLVVIWK QKPRYEIRWR VIESISPDGH EYIYVDPMQL PYDSRWEFPR DGLVLGRVLG 600

SGAFGKVVEG TAYGLSRSQP VMKVAVKMLK PTARSSEKQA LMSELKIMTH LGPHLNIVNL 660

LGACTKSGPI YIITEYCFYG DLVNYLHKNR DSFLSHHPEK PKKELDIFGL NPADESTRSY 720

VILSFENNGD YMDMKQADTT QYVPMLERKE VSKYSDIQRS LYDRPASYKK KSMLDSEVKN 780

LLSDDNSEGL TLLDLLSFTY QVARGMEFLA SKNCVHRDLA ARNVLLAQGK IVKICDFGLA 840

RDIMHDSNYV SKGSTFLPVK WMAPESIFDN LYTTLSDVWS YGILLWEIFS LGGTPYPGMM 900

VDSTFYNKIK SGYRMAKPDH ATSEVYEIMV KCWNSEPEKR PSFYHLSEIV ENLLPGQYKK 960

SYEKIHLDFL KSDHPAVARM RVDSDNAYIG VTYKNEEDKL KDWEGGLDEQ RLSADSGYII 1020

PLPDIDPVPE EEDLGKRNRH SSQTSEESAI ETGSSSSTFI KREDETIEDI DMMDDIGIDS 1080

SDLVEDSFL 1089

Nucleotide Sequence
(FASTA)

ATGGGGACTT CCCATCCGGC GTTCCTGGTC TTAGGCTGTC TTCTCACAGG GCTGAGCCTA 60

ATCCTCTGCC AGCTTTCATT ACCCTCTATC CTTCCAAATG AAAATGAAAA GGTTGTGCAG 120

CTGAATTCAT CCTTTTCTCT GAGATGCTTT GGGGAGAGTG AAGTGAGCTG GCAGTACCCC 180

ATGTCTGAAG AAGAGAGCTC CGATGTGGAA ATCAGAAATG AAGAAAACAA CAGCGGCCTT 240

TTTGTGACGG TCTTGGAAGT GAGCAGTGCC TCGGCGGCCC ACACAGGGTT GTACACTTGC 300

TATTACAACC ACACTCAGAC AGAAGAGAAT GAGCTTGAAG GCAGGCACAT TTACATCTAT 360

GTGCCAGACC CAGATGTAGC CTTTGTACCT CTAGGAATGA CGGATTATTT AGTCATCGTG 420

GAGGATGATG ATTCTGCCAT TATACCTTGT CGCACAACTG ATCCCGAGAC TCCTGTAACC 480

TTACACAACA GTGAGGGGGT GGTACCTGCC TCCTACGACA GCAGACAGGG CTTTAATGGG 540

ACCTTCACTG TAGGGCCCTA TATCTGTGAG GCCACCGTCA AAGGAAAGAA GTTCCAGACC 600

ATCCCATTTA ATGTTTATGC TTTAAAAGCA ACATCAGAGC TGGATCTAGA AATGGAAGCT 660

CTTAAAACCG TGTATAAGTC AGGGGAAACG ATTGTGGTCA CCTGTGCTGT TTTTAACAAT 720

GAGGTGGTTG ACCTTCAATG GACTTACCCT GGAGAAGTGA AAGGCAAAGG CATCACAATG 780

CTGGAAGAAA TCAAAGTCCC ATCCATCAAA TTGGTGTACA CTTTGACGGT CCCCGAGGCC 840

ACGGTGAAAG ACAGTGGAGA TTACGAATGT GCTGCCCGCC AGGCTACCAG GGAGGTCAAA 900

GAAATGAAGA AAGTCACTAT TTCTGTCCAT GAGAAAGGTT TCATTGAAAT CAAACCCACC 960

TTCAGCCAGT TGGAAGCTGT CAACCTGCAT GAAGTCAAAC ATTTTGTTGT AGAGGTGCGG 1020

GCCTACCCAC CTCCCAGGAT ATCCTGGCTG AAAAACAATC TGACTCTGAT TGAAAATCTC 1080

ACTGAGATCA CCACTGATGT GGAAAAGATT CAGGAAATAA GGTATCGAAG CAAATTAAAG 1140

CTGATCCGTG CTAAGGAAGA AGACAGTGGC CATTATACTA TTGTAGCTCA AAATGAAGAT 1200

GCTGTGAAGA GCTATACTTT TGAACTGTTA ACTCAAGTTC CTTCATCCAT TCTGGACTTG 1260

GTCGATGATC ACCATGGCTC AACTGGGGGA CAGACGGTGA GGTGCACAGC TGAAGGCACG 1320

CCGCTTCCTG ATATTGAGTG GATGATATGC AAAGATATTA AGAAATGTAA TAATGAAACT 1380

TCCTGGACTA TTTTGGCCAA CAATGTCTCA AACATCATCA CGGAGATCCA CTCCCGAGAC 1440

AGGAGTACCG TGGAGGGCCG TGTGACTTTC GCCAAAGTGG AGGAGACCAT CGCCGTGCGA 1500

TGCCTGGCTA AGAATCTCCT TGGAGCTGAG AACCGAGAGC TGAAGCTGGT GGCTCCCACC 1560

CTGCGTTCTG AACTCACGGT GGCTGCTGCA GTCCTGGTGC TGTTGGTGAT TGTGATCATC 1620

TCACTTATTG TCCTGGTTGT CATTTGGAAA CAGAAACCGA GGTATGAAAT TCGCTGGAGG 1680

GTCATTGAAT CAATCAGCCC AGATGGACAT GAATATATTT ATGTGGACCC GATGCAGCTG 1740

CCTTATGACT CAAGATGGGA GTTTCCAAGA GATGGACTAG TGCTTGGTCG GGTCTTGGGG 1800

TCTGGAGCGT TTGGGAAGGT GGTTGAAGGA ACAGCCTATG GATTAAGCCG GTCCCAACCT 1860

GTCATGAAAG TTGCAGTGAA GATGCTAAAA CCCACGGCCA GATCCAGTGA AAAACAAGCT 1920

CTCATGTCTG AACTGAAGAT AATGACTCAC CTGGGGCCAC ATTTGAACAT TGTAAACTTG 1980

CTGGGAGCCT GCACCAAGTC AGGCCCCATT TACATCATCA CAGAGTATTG CTTCTATGGA 2040

GATTTGGTCA ACTATTTGCA TAAGAATAGG GATAGCTTCC TGAGCCACCA CCCAGAGAAG 2100

CCAAAGAAAG AGCTGGATAT CTTTGGATTG AACCCTGCTG ATGAAAGCAC ACGGAGCTAT 2160

GTTATTTTAT CTTTTGAAAA CAATGGTGAC TACATGGACA TGAAGCAGGC TGATACTACA 2220

CAGTATGTCC CCATGCTAGA AAGGAAAGAG GTTTCTAAAT ATTCCGACAT CCAGAGATCA 2280

CTCTATGATC GTCCAGCCTC ATATAAGAAG AAATCTATGT TAGACTCAGA AGTCAAAAAC 2340

CTCCTTTCAG ATGATAACTC AGAAGGCCTT ACTTTATTGG ATTTGTTGAG CTTCACCTAT 2400

CAAGTTGCCC GAGGAATGGA GTTTTTGGCT TCAAAAAATT GTGTCCACCG TGATCTGGCT 2460

GCTCGCAACG TCCTCCTGGC ACAAGGAAAA ATTGTGAAGA TCTGTGACTT TGGCCTGGCC 2520

AGAGACATCA TGCATGATTC GAACTATGTG TCGAAAGGCA GTACCTTTCT GCCCGTGAAG 2580

TGGATGGCTC CTGAGAGCAT CTTTGACAAC CTCTACACCA CACTGAGTGA TGTCTGGTCT 2640

TATGGCATTC TGCTCTGGGA GATCTTTTCC CTTGGTGGCA CCCCTTACCC CGGCATGATG 2700

GTGGATTCTA CTTTCTACAA TAAGATCAAG AGTGGGTACC GGATGGCCAA GCCTGACCAC 2760

GCTACCAGTG AAGTCTACGA GATCATGGTG AAATGCTGGA ACAGTGAGCC GGAGAAGAGA 2820

CCCTCCTTTT ACCACCTGAG TGAGATTGTG GAGAATCTGC TGCCTGGACA ATATAAAAAG 2880

AGTTATGAAA AAATTCACCT GGACTTCCTG AAGAGTGACC ATCCTGCTGT GGCACGCATG 2940

CGTGTGGACT CAGACAATGC ATACATTGGT GTCACCTACA AAAACGAGGA AGACAAGCTG 3000

AAGGACTGGG AGGGTGGTCT GGATGAGCAG AGACTGAGCG CTGACAGTGG CTACATCATT 3060

CCTCTGCCTG ACATTGACCC TGTCCCTGAG GAGGAGGACC TGGGCAAGAG GAACAGACAC 3120

AGCTCGCAGA CCTCTGAAGA GAGTGCCATT GAGACGGGTT CCAGCAGTTC CACCTTCATC 3180

AAGAGAGAGG ACGAGACCAT TGAAGACATC GACATGATGG ATGACATCGG CATAGACTCT 3240

TCAGACCTGG TGGAAGACAG CTTCCTGTAA 3270

Domain Profile

S: 1     lelGkvlGsGafGkvveatayGlsksdavlkvavkmlkstarssekealmselkilshlG  60

         l+lG+vlGsGafGkvve+tayGls+s++v+kvavkmlk+tarssek+almselki++hlG

Q: 593   LVLGRVLGSGAFGKVVEGTAYGLSRSQPVMKVAVKMLKPTARSSEKQALMSELKIMTHLG  652

         78**********************************************************

S: 61    ehenivnllGactkgGpiyviteyccyGdllnylrrkresflkkslekkkekeeeldska  120

         +h+nivnllGactk+Gpiy+iteyc+yGdl+nyl+++r+sfl+++ ek   +++eld+++

Q: 653   PHLNIVNLLGACTKSGPIYIITEYCFYGDLVNYLHKNRDSFLSHHPEK---PKKELDIFG  709

         *********************************************999...9999*****

S: 121   l.ladkslrsksslssesseeyvdlkkd................vsdvssskadklasek  163

         l +ad+s+rs+++ls+e++++y+d+k++                +sd+++s +d++as+k

Q: 710   LnPADESTRSYVILSFENNGDYMDMKQAdttqyvpmlerkevskYSDIQRSLYDRPASYK  769

         *9*************************9********************************

S: 164   kksalerevkaaleeedsevleledllsfsyqvakGmeflaskncvhrdlaarnvllteG  223

         kks+l++evk+ l++++se+l+l dllsf+yqva+Gmeflaskncvhrdlaarnvll++G

Q: 770   KKSMLDSEVKNLLSDDNSEGLTLLDLLSFTYQVARGMEFLASKNCVHRDLAARNVLLAQG  829

         ************************************************************

S: 224   kvvkicdfGlardimkdsnyvvkGnarlpvkwmapesifdslytvksdvwsyGillweif  283

         k+vkicdfGlardim+dsnyv+kG+++lpvkwmapesifd+lyt+ sdvwsyGillweif

Q: 830   KIVKICDFGLARDIMHDSNYVSKGSTFLPVKWMAPESIFDNLYTTLSDVWSYGILLWEIF  889

         ************************************************************

S: 284   slGstpypgllvdskfyklikeGyrmaqpayateeiyeimqkcweaepekrpsfkqlvel  343

         slG+tpypg++vds+fy++ik+Gyrma+p++at+e+yeim+kcw++epekrpsf++l+e+

Q: 890   SLGGTPYPGMMVDSTFYNKIKSGYRMAKPDHATSEVYEIMVKCWNSEPEKRPSFYHLSEI  949

         *********************************************************998

S: 344   l  344

Q: 950   V  950

Domain Sequence
(FASTA)

LVLGRVLGSG AFGKVVEGTA YGLSRSQPVM KVAVKMLKPT ARSSEKQALM SELKIMTHLG 60

PHLNIVNLLG ACTKSGPIYI ITEYCFYGDL VNYLHKNRDS FLSHHPEKPK KELDIFGLNP 120

ADESTRSYVI LSFENNGDYM DMKQADTTQY VPMLERKEVS KYSDIQRSLY DRPASYKKKS 180

MLDSEVKNLL SDDNSEGLTL LDLLSFTYQV ARGMEFLASK NCVHRDLAAR NVLLAQGKIV 240

KICDFGLARD IMHDSNYVSK GSTFLPVKWM APESIFDNLY TTLSDVWSYG ILLWEIFSLG 300

GTPYPGMMVD STFYNKIKSG YRMAKPDHAT SEVYEIMVKC WNSEPEKRPS FYHLSEIV 358

Keyword

3D-structure; Alternative splicing; ATP-binding; Cell membrane; Chemotaxis; Complete proteome; Developmental protein; Disulfide bond; Glycoprotein; Host-virus interaction; Immunoglobulin domain; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Polymorphism; Proto-oncogene; Receptor; Reference proteome; Repeat; Signal; Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0005887	; C:integral to plasma membrane
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0005018	; F:platelet-derived growth factor alpha-receptor activity
GO:0048407	; F:platelet-derived growth factor binding
GO:0042803	; F:protein homodimerization activity
GO:0005021	; F:vascular endothelial growth factor-activated receptor activity
GO:0030325	; P:adrenal gland development
GO:0055003	; P:cardiac myofibril assembly
GO:0060326	; P:cell chemotaxis
GO:0071230	; P:cellular response to amino acid stimulus
GO:0007204	; P:elevation of cytosolic calcium ion concentration
GO:0048701	; P:embryonic cranial skeleton morphogenesis
GO:0048557	; P:embryonic digestive tract morphogenesis
GO:0008210	; P:estrogen metabolic process
GO:0030198	; P:extracellular matrix organization
GO:0060325	; P:face morphogenesis
GO:0042063	; P:gliogenesis
GO:0001701	; P:in utero embryonic development
GO:0048839	; P:inner ear development
GO:0033327	; P:Leydig cell differentiation
GO:0030324	; P:lung development
GO:0001553	; P:luteinization
GO:0030539	; P:male genitalia development
GO:0072277	; P:metanephric glomerular capillary formation
GO:0010544	; P:negative regulation of platelet activation
GO:0042475	; P:odontogenesis of dentin-containing tooth
GO:0060021	; P:palate development
GO:0018108	; P:peptidyl-tyrosine phosphorylation
GO:0048015	; P:phosphatidylinositol-mediated signaling
GO:0070527	; P:platelet aggregation
GO:0030335	; P:positive regulation of cell migration
GO:0038091	; P:positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway
GO:0045740	; P:positive regulation of DNA replication
GO:0070374	; P:positive regulation of ERK1 and ERK2 cascade
GO:0048146	; P:positive regulation of fibroblast proliferation
GO:0043552	; P:positive regulation of phosphatidylinositol 3-kinase activity
GO:0014068	; P:positive regulation of phosphatidylinositol 3-kinase cascade
GO:0010863	; P:positive regulation of phospholipase C activity
GO:0046777	; P:protein autophosphorylation
GO:2000249	; P:regulation of actin cytoskeleton reorganization
GO:0050920	; P:regulation of chemotaxis
GO:2000739	; P:regulation of mesenchymal stem cell differentiation
GO:0034097	; P:response to cytokine stimulus
GO:0032355	; P:response to estradiol stimulus
GO:0055093	; P:response to hyperoxia
GO:0010035	; P:response to inorganic substance
GO:0061298	; P:retina vasculature development in camera-type eye
GO:0023019	; P:signal transduction involved in regulation of gene expression
GO:0048010	; P:vascular endothelial growth factor receptor signaling pathway
GO:0019048	; P:virus-host interaction

KEGG

hsa:5156

;

InterPros

IPR007110	; Ig-like.
IPR013783	; Ig-like_fold.
IPR013098	; Ig_I-set.
IPR003599	; Ig_sub.
IPR003598	; Ig_sub2.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.
IPR016243	; Tyr_kinase_CSF1/PDGF_rcpt.
IPR001824	; Tyr_kinase_rcpt_3_CS.
IPR009134	; Tyr_kinase_VEGFR_rcpt_N.

Pfam

PF07679	; I-set; 2.
PF07714	; Pkinase_Tyr; 1.

SMARTs

SM00409	; IG; 2.
SM00408	; IGc2; 1.
SM00219	; TyrKc; 1.

Prosites

PS50835	; IG_LIKE; 2.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS00240	; RECEPTOR_TYR_KIN_III; 1.

Prints

PR01832

; VEGFRECEPTOR.

Created Date

20-Feb-2013