EKS-HOS-00183

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00183

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CMGC/SRPK	884.4	1.1E-265	81	686	606

Status

Reviewed

Ensembl Protein

ENSP00000349863

UniProt Accession

P78362; A8MVX2; O75220; O75221; Q6NUL0; Q6V1X2; Q8IYQ3;

Protein Name

SRSF protein kinase 2 C-terminal

Protein Synonyms/Alias

SFRS protein kinase 2; Serine/arginine-rich protein-specific kinase 2; SR-protein-specific kinase 2;

Gene Name

SRPK2

Gene Synonyms/Alias

SRPK2;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000135250	ENSP00000417446	ENST00000465112
ENSG00000135250	ENSP00000349863	ENST00000357311
ENSG00000135250	ENSP00000417357	ENST00000460391
ENSG00000135250	ENSP00000417946	ENST00000465072
ENSG00000135250	ENSP00000420208	ENST00000477925
ENSG00000135250	ENSP00000418786	ENST00000474770
ENSG00000135250	ENSP00000377262	ENST00000393651
ENSG00000135250	ENSP00000419240	ENST00000482897
ENSG00000135250	ENSP00000419791	ENST00000489828

Organism

Homo sapiens

Functional Description

Serine/arginine-rich protein-specific kinase whichspecifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression. This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression. Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up- regulation. Plays an essential role in splicesomal B complex formation via the phosphorylation of DDX23/PRP28. Can mediate hepatitis B virus (HBV) core protein phosphorylation. Plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles.

Protein Length

688

Protein Sequence
(FASTA)

MSVNSEKSSS SERPEPQQKA PLVPPPPPPP PPPPPPLPDP TPPEPEEEIL GSDDEEQEDP 60

ADYCKGGYHP VKIGDLFNGR YHVIRKLGWG HFSTVWLCWD MQGKRFVAMK VVKSAQHYTE 120

TALDEIKLLK CVRESDPSDP NKDMVVQLID DFKISGMNGI HVCMVFEVLG HHLLKWIIKS 180

NYQGLPVRCV KSIIRQVLQG LDYLHSKCKI IHTDIKPENI LMCVDDAYVR RMAAEATEWQ 240

KAGAPPPSGS AVSTAPQQKP IGKISKNKKK KLKKKQKRQA ELLEKRLQEI EELEREAERK 300

IIEENITSAA PSNDQDGEYC PEVKLKTTGL EEAAEAETAK DNGEAEDQEE KEDAEKENIE 360

KDEDDVDQEL ANIDPTWIES PKTNGHIENG PFSLEQQLDD EDDDEEDCPN PEEYNLDEPN 420

AESDYTYSSS YEQFNGELPN GRHKIPESQF PEFSTSLFSG SLEPVACGSV LSEGSPLTEQ 480

EESSPSHDRS RTVSASSTGD LPKAKTRAAD LLVNPLDPRN ADKIRVKIAD LGNACWVHKH 540

FTEDIQTRQY RSIEVLIGAG YSTPADIWST ACMAFELATG DYLFEPHSGE DYSRDEDHIA 600

HIIELLGSIP RHFALSGKYS REFFNRRGEL RHITKLKPWS LFDVLVEKYG WPHEDAAQFT 660

DFLIPMLEMV PEKRASAGEC LRHPWLNS 688

Nucleotide Sequence
(FASTA)

ATGTCAGTTA ACTCTGAGAA GTCGTCCTCT TCAGAAAGGC CGGAGCCTCA ACAGAAAGCT 60

CCTTTAGTTC CTCCTCCTCC ACCGCCACCA CCACCACCAC CGCCACCTTT GCCAGACCCC 120

ACACCCCCGG AGCCAGAGGA GGAGATCCTG GGATCAGATG ATGAGGAGCA AGAGGACCCT 180

GCGGACTACT GCAAAGGTGG ATATCATCCA GTGAAAATTG GAGACCTCTT CAATGGCCGG 240

TATCATGTTA TTAGAAAGCT TGGATGGGGG CACTTCTCTA CTGTCTGGCT GTGCTGGGAT 300

ATGCAGGGGA AAAGATTTGT TGCAATGAAA GTTGTAAAAA GTGCCCAGCA TTATACGGAG 360

ACAGCCTTGG ATGAAATAAA ATTGCTCAAA TGTGTTCGAG AAAGTGATCC CAGTGACCCA 420

AACAAAGACA TGGTGGTCCA GCTCATTGAC GACTTCAAGA TTTCAGGCAT GAATGGGATA 480

CATGTCTGCA TGGTCTTCGA AGTACTTGGC CACCATCTCC TCAAGTGGAT CATCAAATCC 540

AACTATCAAG GCCTCCCAGT ACGTTGTGTG AAGAGTATCA TTCGACAGGT CCTTCAAGGG 600

TTAGATTACT TACACAGTAA GTGCAAGATC ATTCATACTG ACATAAAGCC GGAAAATATC 660

TTGATGTGTG TGGATGATGC ATATGTGAGA AGAATGGCAG CTGAGGCCAC TGAGTGGCAG 720

AAAGCAGGTG CTCCTCCTCC TTCAGGGTCT GCAGTGAGTA CGGCTCCACA GCAGAAACCT 780

ATAGGAAAAA TATCTAAAAA CAAAAAGAAA AAACTGAAAA AGAAACAGAA GAGGCAGGCT 840

GAGTTATTGG AGAAGCGCCT GCAGGAGATA GAAGAATTGG AGCGAGAAGC TGAAAGGAAA 900

ATAATAGAAG AAAACATCAC CTCAGCTGCA CCTTCCAATG ACCAGGATGG CGAATACTGC 960

CCAGAGGTGA AACTAAAAAC AACAGGATTA GAGGAGGCGG CTGAGGCAGA GACTGCAAAG 1020

GACAATGGTG AAGCTGAGGA CCAGGAAGAG AAAGAAGATG CTGAGAAAGA AAACATTGAA 1080

AAAGATGAAG ATGATGTAGA TCAGGAACTT GCGAACATAG ACCCTACGTG GATAGAATCA 1140

CCTAAAACCA ATGGCCATAT TGAGAATGGC CCATTCTCAC TGGAGCAGCA ACTGGACGAT 1200

GAAGATGATG ATGAAGAAGA CTGCCCAAAT CCTGAGGAAT ATAATCTTGA TGAGCCAAAT 1260

GCAGAAAGTG ATTACACATA TAGCAGCTCC TATGAACAAT TCAATGGTGA ATTGCCAAAT 1320

GGACGACATA AAATTCCCGA GTCACAGTTC CCAGAGTTTT CCACCTCGTT GTTCTCTGGA 1380

TCCTTAGAAC CTGTGGCCTG CGGCTCTGTG CTTTCTGAGG GATCACCACT TACTGAGCAA 1440

GAGGAGAGCA GTCCATCCCA TGACAGAAGC AGAACGGTTT CAGCCTCCAG TACTGGGGAT 1500

TTGCCAAAAG CAAAAACCCG GGCAGCTGAC TTGTTGGTGA ATCCCCTGGA TCCGCGGAAT 1560

GCAGATAAAA TTAGAGTAAA AATTGCTGAC CTGGGAAATG CTTGTTGGGT GCATAAACAC 1620

TTCACGGAAG ACATCCAGAC GCGTCAGTAC CGCTCCATAG AGGTTTTAAT AGGAGCGGGG 1680

TACAGCACCC CTGCGGACAT CTGGAGCACG GCGTGTATGG CATTTGAGCT GGCAACGGGA 1740

GATTATTTGT TTGAACCACA TTCTGGGGAA GACTATTCCA GAGACGAAGA CCACATAGCC 1800

CACATCATAG AGCTGCTAGG CAGTATTCCA AGGCACTTTG CTCTATCTGG AAAATATTCT 1860

CGGGAATTCT TCAATCGCAG AGGAGAACTG CGACACATCA CCAAGCTGAA GCCCTGGAGC 1920

CTCTTTGATG TACTTGTGGA AAAGTATGGC TGGCCCCATG AAGATGCTGC ACAGTTTACA 1980

GATTTCCTGA TCCCGATGTT AGAAATGGTT CCAGAAAAAC GAGCCTCAGC TGGCGAATGC 2040

CTTCGGCATC CTTGGTTGAA TTCTTAG 2067

Domain Profile

S: 1     yhvirkLGWGhfstvWLawdlqekrfvalkvvksaehytetaldeikllksvresdpsdp  60

         yhvirkLGWGhfstvWL+wd+q+krfva+kvvksa+hytetaldeikllk+vresdpsdp

Q: 81    YHVIRKLGWGHFSTVWLCWDMQGKRFVAMKVVKSAQHYTETALDEIKLLKCVRESDPSDP  140

         9***********************************************************

S: 61    krekvvqllddfkisGvnGvhvclvfevLGenLlkliiksnyrGlpleqvkkiirqvLeg  120

         ++++vvql+ddfkisG+nG+hvc+vfevLG++Llk+iiksny+Glp+++vk+iirqvL+g

Q: 141   NKDMVVQLIDDFKISGMNGIHVCMVFEVLGHHLLKWIIKSNYQGLPVRCVKSIIRQVLQG  200

         ************************************************************

S: 121   LdylhekCkiihtdikPenvLlavddeyirrlaaeatewqkagakppsgsavstapqkka  180

         Ldylh+kCkiihtdikPen+L++vdd+y+rr+aaeatewqkaga+ppsgsavstapq+k+

Q: 201   LDYLHSKCKIIHTDIKPENILMCVDDAYVRRMAAEATEWQKAGAPPPSGSAVSTAPQQKP  260

         ************************************************************

S: 181   ieklsknkkkklkkkqkrqaellekrlqeleele..........................  214

         i+k+sknkkkklkkkqkrqaellekrlqe+eele

Q: 261   IGKISKNKKKKLKKKQKRQAELLEKRLQEIEELEreaerkiieenitsaapsndqdgeyc  320

         ************************************************************

S: 215   .................aaeanseaedqelkedaekgeleeieadveeevanedea....  253

                          +a++n+eaedqe+kedaek+++e++e+dv++e+an+d++

Q: 321   pevklkttgleeaaeaeTAKDNGEAEDQEEKEDAEKENIEKDEDDVDQELANIDPTwies  380

         *****************9**************************************9988

S: 254   ...................seeee....................essltsssdaesksge  274

                            +   e                    es++t+ss++e+++ge

Q: 381   pktnghiengpfsleqqldD---EdddeedcpnpeeynldepnaESDYTYSSSYEQFNGE  437

         88888888888877776651...144556788888888888899****************

S: 275   l............seastellsesleqlacgsvls.........................  297

         l            +e+st+l+s+sle++acgsvls

Q: 438   LpngrhkipesqfPEFSTSLFSGSLEPVACGSVLSegsplteqeesspshdrsrtvsass  497

         **********9999**********************************************

S: 298   ...............vnplepknadkikvkiadLGnaCwvhkhftediqtrqyrslevli  342

                        vnpl+p+nadki+vkiadLGnaCwvhkhftediqtrqyrs+evli

Q: 498   tgdlpkaktraadllVNPLDPRNADKIRVKIADLGNACWVHKHFTEDIQTRQYRSIEVLI  557

         ************************************************************

S: 343   GagygtpadiWstaClafeLatGdyLfephsgedysrdedhiakiiellGkiPrklilkG  402

         Gagy+tpadiWstaC+afeLatGdyLfephsgedysrdedhia+iiellG+iPr+++l+G

Q: 558   GAGYSTPADIWSTACMAFELATGDYLFEPHSGEDYSRDEDHIAHIIELLGSIPRHFALSG  617

         ************************************************************

S: 403   kysreffnkkgeLrhitkLkpwsLlevLvekyewskeeaaefsdfLlPmleldpekrasa  462

         kysreffn++geLrhitkLkpwsL++vLveky+w++e+aa+f+dfL+Pmle++pekrasa

Q: 618   KYSREFFNRRGELRHITKLKPWSLFDVLVEKYGWPHEDAAQFTDFLIPMLEMVPEKRASA  677

         ************************************************************

S: 463   aeclkhpwL  471

         +ecl+hpwL

Q: 678   GECLRHPWL  686

         ********8

Domain Sequence
(FASTA)

YHVIRKLGWG HFSTVWLCWD MQGKRFVAMK VVKSAQHYTE TALDEIKLLK CVRESDPSDP 60

NKDMVVQLID DFKISGMNGI HVCMVFEVLG HHLLKWIIKS NYQGLPVRCV KSIIRQVLQG 120

LDYLHSKCKI IHTDIKPENI LMCVDDAYVR RMAAEATEWQ KAGAPPPSGS AVSTAPQQKP 180

IGKISKNKKK KLKKKQKRQA ELLEKRLQEI EELEREAERK IIEENITSAA PSNDQDGEYC 240

PEVKLKTTGL EEAAEAETAK DNGEAEDQEE KEDAEKENIE KDEDDVDQEL ANIDPTWIES 300

PKTNGHIENG PFSLEQQLDD EDDDEEDCPN PEEYNLDEPN AESDYTYSSS YEQFNGELPN 360

GRHKIPESQF PEFSTSLFSG SLEPVACGSV LSEGSPLTEQ EESSPSHDRS RTVSASSTGD 420

LPKAKTRAAD LLVNPLDPRN ADKIRVKIAD LGNACWVHKH FTEDIQTRQY RSIEVLIGAG 480

YSTPADIWST ACMAFELATG DYLFEPHSGE DYSRDEDHIA HIIELLGSIP RHFALSGKYS 540

REFFNRRGEL RHITKLKPWS LFDVLVEKYG WPHEDAAQFT DFLIPMLEMV PEKRASAGEC 600

LRHPWL 606

Keyword

3D-structure; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Differentiation; Kinase; mRNA processing; mRNA splicing; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0005730	; C:nucleolus
GO:0071889	; F:14-3-3 protein binding
GO:0005524	; F:ATP binding
GO:0000287	; F:magnesium ion binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0001525	; P:angiogenesis
GO:0030154	; P:cell differentiation
GO:0045087	; P:innate immune response
GO:0007243	; P:intracellular protein kinase cascade
GO:0045071	; P:negative regulation of viral genome replication
GO:0035063	; P:nuclear speck organization
GO:0045787	; P:positive regulation of cell cycle
GO:0008284	; P:positive regulation of cell proliferation
GO:0010628	; P:positive regulation of gene expression
GO:0043525	; P:positive regulation of neuron apoptotic process
GO:0045070	; P:positive regulation of viral genome replication
GO:0048024	; P:regulation of mRNA splicing, via spliceosome
GO:0000245	; P:spliceosomal complex assembly

KEGG

hsa:6733

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 2.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013