EKS-HOS-00074

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00074

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CMGC/CDK	338.8	1.2E-100	6	295	290

Status

Reviewed

Ensembl Protein

ENSP00000257904

UniProt Accession

P11802; O00576; Q6FG61;

Protein Name

Cyclin-dependent kinase 4

Protein Synonyms/Alias

Cell division protein kinase 4; PSK-J3;

Gene Name

CDK4

Gene Synonyms/Alias

CDK4;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000135446	ENSP00000447779	ENST00000546489
ENSG00000135446	ENSP00000449725	ENST00000547853
ENSG00000135446	ENSP00000316889	ENST00000312990
ENSG00000135446	ENSP00000448098	ENST00000550419
ENSG00000135446	ENSP00000257904	ENST00000257904
ENSG00000135446	ENSP00000447274	ENST00000547281
ENSG00000135446	ENSP00000449391	ENST00000551800
ENSG00000135446	ENSP00000448963	ENST00000552388
ENSG00000135446	ENSP00000448885	ENST00000553237
ENSG00000135446	ENSP00000447005	ENST00000549606
ENSG00000135446	ENSP00000439076	ENST00000540325
ENSG00000135446	ENSP00000446763	ENST00000552862
ENSG00000135446	ENSP00000449179	ENST00000552254

Organism

Homo sapiens

Functional Description

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexesthat phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Protein Length

303

Protein Sequence
(FASTA)

MATSRYEPVA EIGVGAYGTV YKARDPHSGH FVALKSVRVP NGGGGGGGLP ISTVREVALL 60

RRLEAFEHPN VVRLMDVCAT SRTDREIKVT LVFEHVDQDL RTYLDKAPPP GLPAETIKDL 120

MRQFLRGLDF LHANCIVHRD LKPENILVTS GGTVKLADFG LARIYSYQMA LTPVVVTLWY 180

RAPEVLLQST YATPVDMWSV GCIFAEMFRR KPLFCGNSEA DQLGKIFDLI GLPPEDDWPR 240

DVSLPRGAFP PRGPRPVQSV VPEMEESGAQ LLLEMLTFNP HKRISAFRAL QHSYLHKDEG 300

NPE 303

Nucleotide Sequence
(FASTA)

ATGGCTACCT CTCGATATGA GCCAGTGGCT GAAATTGGTG TCGGTGCCTA TGGGACAGTG 60

TACAAGGCCC GTGATCCCCA CAGTGGCCAC TTTGTGGCCC TCAAGAGTGT GAGAGTCCCC 120

AATGGAGGAG GAGGTGGAGG AGGCCTTCCC ATCAGCACAG TTCGTGAGGT GGCTTTACTG 180

AGGCGACTGG AGGCTTTTGA GCATCCCAAT GTTGTCCGGC TGATGGACGT CTGTGCCACA 240

TCCCGAACTG ACCGGGAGAT CAAGGTAACC CTGGTGTTTG AGCATGTAGA CCAGGACCTA 300

AGGACATATC TGGACAAGGC ACCCCCACCA GGCTTGCCAG CCGAAACGAT CAAGGATCTG 360

ATGCGCCAGT TTCTAAGAGG CCTAGATTTC CTTCATGCCA ATTGCATCGT TCACCGAGAT 420

CTGAAGCCAG AGAACATTCT GGTGACAAGT GGTGGAACAG TCAAGCTGGC TGACTTTGGC 480

CTGGCCAGAA TCTACAGCTA CCAGATGGCA CTTACACCCG TGGTTGTTAC ACTCTGGTAC 540

CGAGCTCCCG AAGTTCTTCT GCAGTCCACA TATGCAACAC CTGTGGACAT GTGGAGTGTT 600

GGCTGTATCT TTGCAGAGAT GTTTCGTCGA AAGCCTCTCT TCTGTGGAAA CTCTGAAGCC 660

GACCAGTTGG GCAAAATCTT TGACCTGATT GGGCTGCCTC CAGAGGATGA CTGGCCTCGA 720

GATGTATCCC TGCCCCGTGG AGCCTTTCCC CCCAGAGGGC CCCGCCCAGT GCAGTCGGTG 780

GTACCTGAGA TGGAGGAGTC GGGAGCACAG CTGCTGCTGG AAATGCTGAC TTTTAACCCA 840

CACAAGCGAA TCTCTGCCTT TCGAGCTCTG CAGCACTCTT ATCTACATAA GGATGAAGGT 900

AATCCGGAGT GA 912

Domain Profile

S: 1     yeklekigeGtygkvykakdketgevvAlKkirlekeke...gvpitalrEisllkel..  55

         ye +++ig G+yg+vyka+d ++g++vAlK++r+ +      g+pi+++rE++ll++l

Q: 6     YEPVAEIGVGAYGTVYKARDPHSGHFVALKSVRVPNGGGgggGLPISTVREVALLRRLea  65

         88899******************************98744449**************988

S: 56    .khknivkLlevva....kdkkklyLvfefleqdLkkllkkkkkkklsleevkslllqll  110

          +h+n+v+L++v+a     ++ k++Lvfe+++qdL ++l+k +  +l+ e++k l++q+l

Q: 66    fEHPNVVRLMDVCAtsrtDREIKVTLVFEHVDQDLRTYLDKAPPPGLPAETIKDLMRQFL  125

         89************887444589*************************************

S: 111   eglaylHsnkilHRDlKpqNlLiskegklklaDfGlarafssplktytkevvTlwYraPe  170

         +gl++lH+n i+HRDlKp+N+L+++ g++klaDfGlar +s ++  +t++vvTlwYraPe

Q: 126   RGLDFLHANCIVHRDLKPENILVTSGGTVKLADFGLARIYSYQMA-LTPVVVTLWYRAPE  184

         ******************************************999.**************

S: 171   lLlgakeYstavDiWsvgcilaelltrkplfqgkseidqlerifkllgtpsekvwpevsk  230

         +Ll+++ Y t vD+Wsvgci+ae+++rkplf g+se dql +if+l+g p e++wp+  +

Q: 185   VLLQST-YATPVDMWSVGCIFAEMFRRKPLFCGNSEADQLGKIFDLIGLPPEDDWPRDVS  243

         ***985.*****************************************************

S: 231   lpeykksfpkkkkkklkekvkklkekaldllekllaldpkkRisakealqhkyf  284

         lp+    fp++ ++ ++++v++++e++ +ll ++l+++p+kRisa +alqh+y+

Q: 244   LPRGA--FPPRGPRPVQSVVPEMEESGAQLLLEMLTFNPHKRISAFRALQHSYL  295

         ***65..*********************************************96

Domain Sequence
(FASTA)

YEPVAEIGVG AYGTVYKARD PHSGHFVALK SVRVPNGGGG GGGLPISTVR EVALLRRLEA 60

FEHPNVVRLM DVCATSRTDR EIKVTLVFEH VDQDLRTYLD KAPPPGLPAE TIKDLMRQFL 120

RGLDFLHANC IVHRDLKPEN ILVTSGGTVK LADFGLARIY SYQMALTPVV VTLWYRAPEV 180

LLQSTYATPV DMWSVGCIFA EMFRRKPLFC GNSEADQLGK IFDLIGLPPE DDWPRDVSLP 240

RGAFPPRGPR PVQSVVPEME ESGAQLLLEM LTFNPHKRIS AFRALQHSYL 290

Keyword

3D-structure; Acetylation; ATP-binding; Cell cycle; Cell division; Complete proteome; Cytoplasm; Disease mutation; Kinase; Membrane; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Macropus eugenii"; ?>Microcebus murinus"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sorex araneus"; ?>Sus scrofa"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0000785	; C:chromatin
GO:0000307	; C:cyclin-dependent protein kinase holoenzyme complex
GO:0005829	; C:cytosol
GO:0031965	; C:nuclear membrane
GO:0005730	; C:nucleolus
GO:0005654	; C:nucleoplasm
GO:0005667	; C:transcription factor complex
GO:0005524	; F:ATP binding
GO:0004693	; F:cyclin-dependent protein kinase activity
GO:0051301	; P:cell division
GO:0000080	; P:G1 phase of mitotic cell cycle
GO:0000082	; P:G1/S transition of mitotic cell cycle
GO:0048146	; P:positive regulation of fibroblast proliferation
GO:0051726	; P:regulation of cell cycle
GO:0010468	; P:regulation of gene expression
GO:0042493	; P:response to drug
GO:0000084	; P:S phase of mitotic cell cycle
GO:0007165	; P:signal transduction

KEGG

hsa:1019

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013