EKS-HOS-00109

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00109

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/Tec	491.8	1.7E-147	370	620	251

Status

Reviewed

Ensembl Protein

ENSP00000370912

UniProt Accession

P42680; B7ZKZ6; Q3MIS5;

Protein Name

Tyrosine-protein kinase Tec

Protein Synonyms/Alias

Gene Name

TEC

Gene Synonyms/Alias

TEC; PSCTK4;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000135605	ENSP00000420836	ENST00000506752
ENSG00000135605	ENSP00000424365	ENST00000515146
ENSG00000135605	ENSP00000424567	ENST00000505452
ENSG00000135605	ENSP00000370912	ENST00000381501

Organism

Homo sapiens

Functional Description

Non-receptor tyrosine kinase that contributes tosignaling from many receptors and participates as a signal transducer in multiple downstream pathways, including regulation of the actin cytoskekleton. Plays a redundant role to ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. Required for TCR-dependent IL2 gene induction. Phosphorylates DOK1, one CD28-specific substrate, and contributes to CD28-signaling. Mediates signals that negatively regulate IL2RA expression induced by TCR cross-linking. Plays a redundant role to BTK in BCR-signaling for B-cell development and activation, especially by phosphorylating STAP1, a BCR-signaling protein. Required in mast cells for efficient cytokine production. Involved in both growth and differentiation mechanisms of myeloid cells through activation by the granulocyte colony-stimulating factor CSF3, a critical cytokine to promoting the growth, differentiation, and functional activation of myeloid cells. Participates in platelet signaling downstream of integrin activation. Cooperates with JAK2 through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. GRB10, a negative modifier of the FOS activation pathway, is another substrate of TEC. TEC is involved in G protein-coupled receptor- and integrin-mediated signalings in blood platelets. Plays a role in hepatocyte proliferation and liver regeneration and is involved in HGF-induced ERK signaling pathway. TEC regulates also FGF2 unconventional secretion (endoplasmic reticulum (ER)/Golgi-independent mechanism) under various physiological conditions through phosphorylation of FGF2 'Tyr-215'. May also be involved in the regulation of osteoclast differentiation.

Protein Length

631

Protein Sequence
(FASTA)

MNFNTILEEI LIKRSQQKKK TSPLNYKERL FVLTKSMLTY YEGRAEKKYR KGFIDVSKIK 60

CVEIVKNDDG VIPCQNKYPF QVVHDANTLY IFAPSPQSRD LWVKKLKEEI KNNNNIMIKY 120

HPKFWTDGSY QCCRQTEKLA PGCEKYNLFE SSIRKALPPA PETKKRRPPP PIPLEEEDNS 180

EEIVVAMYDF QAAEGHDLRL ERGQEYLILE KNDVHWWRAR DKYGNEGYIP SNYVTGKKSN 240

NLDQYEWYCR NMNRSKAEQL LRSEDKEGGF MVRDSSQPGL YTVSLYTKFG GEGSSGFRHY 300

HIKETTTSPK KYYLAEKHAF GSIPEIIEYH KHNAAGLVTR LRYPVSVKGK NAPTTAGFSY 360

EKWEINPSEL TFMRELGSGL FGVVRLGKWR AQYKVAIKAI REGAMCEEDF IEEAKVMMKL 420

THPKLVQLYG VCTQQKPIYI VTEFMERGCL LNFLRQRQGH FSRDVLLSMC QDVCEGMEYL 480

ERNSFIHRDL AARNCLVSEA GVVKVSDFGM ARYVLDDQYT SSSGAKFPVK WCPPEVFNYS 540

RFSSKSDVWS FGVLMWEVFT EGRMPFEKYT NYEVVTMVTR GHRLYQPKLA SNYVYEVMLR 600

CWQEKPEGRP SFEDLLRTID ELVECEETFG R 631

Nucleotide Sequence
(FASTA)

ATGAATTTTA ACACTATTTT GGAGGAGATT CTTATTAAAA GGTCACAGCA GAAAAAGAAG 60

ACATCGCCCT TAAACTACAA AGAGAGACTT TTTGTACTTA CAAAGTCCAT GCTAACCTAC 120

TATGAGGGTC GAGCAGAGAA GAAATACAGA AAGGGGTTTA TTGATGTTTC AAAAATCAAG 180

TGTGTGGAAA TAGTGAAGAA TGATGATGGT GTCATTCCCT GTCAAAATAA GTATCCATTT 240

CAGGTTGTTC ATGATGCTAA CACACTTTAC ATTTTTGCAC CTAGTCCACA AAGCAGGGAC 300

CTGTGGGTGA AGAAGTTAAA AGAAGAAATA AAGAACAACA ATAATATTAT GATTAAATAT 360

CATCCTAAAT TCTGGACAGA TGGAAGTTAT CAGTGTTGTA GACAAACTGA AAAATTAGCA 420

CCCGGATGTG AAAAATACAA TCTTTTTGAG AGCAGTATAA GAAAAGCACT ACCTCCAGCA 480

CCAGAAACAA AGAAGCGAAG GCCTCCCCCA CCAATTCCAC TAGAAGAAGA AGATAATAGT 540

GAAGAAATCG TTGTAGCCAT GTATGATTTC CAAGCAGCAG AAGGACATGA TCTCAGATTA 600

GAGAGAGGCC AAGAGTATCT CATTTTAGAA AAGAATGATG TTCATTGGTG GAGAGCAAGA 660

GATAAATATG GGAATGAAGG ATATATCCCA AGTAATTACG TAACGGGAAA GAAATCAAAC 720

AACTTAGATC AATATGAATG GTATTGCAGA AATATGAATA GAAGCAAGGC AGAGCAACTC 780

CTCCGCAGTG AAGATAAAGA AGGTGGTTTT ATGGTAAGGG ATTCCAGTCA ACCAGGCTTG 840

TACACAGTCT CCCTTTATAC CAAGTTTGGA GGAGAAGGTT CATCGGGTTT TAGGCATTAT 900

CATATAAAGG AAACAACAAC ATCTCCAAAG AAGTATTACC TAGCTGAAAA ACATGCTTTT 960

GGCTCCATTC CTGAGATTAT TGAATATCAT AAGCACAATG CAGCAGGACT TGTCACCAGG 1020

CTTCGGTACC CAGTTAGTGT GAAAGGGAAG AATGCACCCA CCACTGCAGG ATTCAGCTAT 1080

GAGAAATGGG AGATTAACCC TTCAGAACTG ACCTTTATGA GGGAATTGGG AAGTGGACTG 1140

TTTGGAGTGG TGAGGCTTGG CAAATGGCGA GCCCAGTACA AAGTCGCAAT CAAAGCTATT 1200

CGGGAAGGTG CAATGTGCGA GGAGGACTTT ATAGAAGAAG CTAAAGTGAT GATGAAACTG 1260

ACACACCCGA AGTTAGTGCA GCTTTATGGT GTGTGCACCC AGCAGAAACC AATATACATT 1320

GTTACTGAGT TCATGGAAAG GGGCTGCCTT CTGAATTTCC TCCGACAGAG ACAAGGTCAT 1380

TTCAGTAGAG ACGTACTGCT GAGCATGTGT CAGGATGTGT GTGAAGGGAT GGAGTATCTG 1440

GAGAGAAACA GCTTCATCCA CAGAGATCTG GCTGCCAGAA ATTGTCTAGT AAGTGAGGCG 1500

GGAGTTGTAA AAGTATCTGA TTTTGGAATG GCCAGGTATG TTCTGGATGA TCAGTACACA 1560

AGTTCTTCTG GTGCTAAGTT TCCTGTGAAG TGGTGTCCAC CTGAAGTGTT TAATTACAGC 1620

CGCTTCAGCA GCAAATCAGA TGTCTGGTCA TTTGGTGTTT TAATGTGGGA AGTATTCACG 1680

GAAGGCAGAA TGCCTTTTGA AAAATACACC AATTATGAAG TGGTAACCAT GGTTACTCGA 1740

GGCCACCGAC TCTACCAGCC GAAGTTGGCG TCCAACTATG TGTATGAGGT GATGCTGAGA 1800

TGTTGGCAGG AGAAACCAGA GGGAAGGCCT TCTTTCGAAG ATCTGCTGCG CACAATAGAT 1860

GAACTAGTTG AATGTGAAGA AACTTTTGGA AGATAA 1896

Domain Profile

S: 1     ltllkelgsgqfgvvklgkwrakidvaikaikegamseedfieeakvmmklshpklvqly  60

         lt+++elgsg fgvv+lgkwra+++vaikai+egam+eedfieeakvmmkl+hpklvqly

Q: 370   LTFMRELGSGLFGVVRLGKWRAQYKVAIKAIREGAMCEEDFIEEAKVMMKLTHPKLVQLY  429

         79**********************************************************

S: 61    gvclkqkpiyivteylengcllnylrekkkklekelllemckdvcegmeylerksfihrd  120

         gvc++qkpiyivte++e+gclln+lr++++++++++ll+mc+dvcegmeyler+sfihrd

Q: 430   GVCTQQKPIYIVTEFMERGCLLNFLRQRQGHFSRDVLLSMCQDVCEGMEYLERNSFIHRD  489

         ************************************************************

S: 121   laarnclvdeelvvkvsdfglaryvlddqyvssvgakfpvkwsppevlsyskyssksdvw  180

         laarnclv+e+ vvkvsdfg+aryvlddqy+ss+gakfpvkw+ppev++ys++ssksdvw

Q: 490   LAARNCLVSEAGVVKVSDFGMARYVLDDQYTSSSGAKFPVKWCPPEVFNYSRFSSKSDVW  549

         ************************************************************

S: 181   afgvlmwevfsegklpyekksnsevvekvsrglrlyrpklaskkvyevmkscwkekpeer  240

         +fgvlmwevf+eg++p+ek++n+evv++v+rg+rly+pklas++vyevm +cw+ekpe+r

Q: 550   SFGVLMWEVFTEGRMPFEKYTNYEVVTMVTRGHRLYQPKLASNYVYEVMLRCWQEKPEGR  609

         ************************************************************

S: 241   ptfkellsale  251

         p+f++ll+++

Q: 610   PSFEDLLRTID  620

         ********995

Domain Sequence
(FASTA)

LTFMRELGSG LFGVVRLGKW RAQYKVAIKA IREGAMCEED FIEEAKVMMK LTHPKLVQLY 60

GVCTQQKPIY IVTEFMERGC LLNFLRQRQG HFSRDVLLSM CQDVCEGMEY LERNSFIHRD 120

LAARNCLVSE AGVVKVSDFG MARYVLDDQY TSSSGAKFPV KWCPPEVFNY SRFSSKSDVW 180

SFGVLMWEVF TEGRMPFEKY TNYEVVTMVT RGHRLYQPKL ASNYVYEVML RCWQEKPEGR 240

PSFEDLLRTI D 251

Keyword

3D-structure; Adaptive immunity; ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Immunity; Kinase; Lipid-binding; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; SH2 domain; SH3 domain; Transferase; Tyrosine-protein kinase; Zinc; Zinc-finger.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dasypus novemcinctus"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005856	; C:cytoskeleton
GO:0005829	; C:cytosol
GO:0005886	; C:plasma membrane
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0004715	; F:non-membrane spanning protein tyrosine kinase activity
GO:0005543	; F:phospholipid binding
GO:0050853	; P:B cell receptor signaling pathway
GO:0007229	; P:integrin-mediated signaling pathway
GO:0007243	; P:intracellular protein kinase cascade
GO:0010543	; P:regulation of platelet activation

KEGG

hsa:7006

;

InterPros

IPR011009	; Kinase-like_dom.
IPR011993	; PH_like_dom.
IPR001849	; Pleckstrin_homology.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR000980	; SH2.
IPR001452	; SH3_domain.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.
IPR001562	; Znf_Btk_motif.

Pfam

PF00779	; BTK; 1.
PF00169	; PH; 1.
PF07714	; Pkinase_Tyr; 1.
PF00017	; SH2; 1.
PF00018	; SH3_1; 1.

SMARTs

SM00107	; BTK; 1.
SM00233	; PH; 1.
SM00252	; SH2; 1.
SM00326	; SH3; 1.
SM00219	; TyrKc; 1.

Prosites

PS50003	; PH_DOMAIN; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS50001	; SH2; 1.
PS50002	; SH3; 1.
PS51113	; ZF_BTK; 1.

Prints

PR00401	; SH2DOMAIN.
PR00452	; SH3DOMAIN.
PR00402	; TECBTKDOMAIN.
PR00109	; TYRKINASE.

Created Date

20-Feb-2013