| Tag | Content |
|---|
| EKPD ID | EKS-HOS-00379 |
| Classification | | Group/Family | Score | E-Value | Start | End | Domain Length |
|---|
| TK/Src | 424.0 | 9.3E-127 | 170 | 419 | 250 |
|
| Status | Reviewed |
| Ensembl Protein | ENSP00000433663 |
| UniProt Accession | P51451; Q16291; Q96IN1; |
| Protein Name | Tyrosine-protein kinase Blk |
| Protein Synonyms/Alias | B lymphocyte kinase; p55-Blk; |
| Gene Name | BLK |
| Gene Synonyms/Alias | BLK; |
| Ensembl Information | |
| Organism | Homo sapiens |
| Functional Description | Non-receptor tyrosine kinase involved in B-lymphocytedevelopment, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulines and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor. Specifically binds and phosphorylates CD79A at 'Tyr-188'and 'Tyr-199', as well as CD79B at 'Tyr-196' and 'Tyr- 207'. Phosphorylates also the immunoglobuline G receptors FCGR2A, FCGR2B and FCGR2C. With FYN and LYN, plays an essential role in pre-B-cell receptor (pre-BCR)-mediated NF-kappa-B activation. Contributes also to BTK activation by indirectly stimulating BTK intramolecular autophosphorylation. In pancreatic islets, acts as a modulator of beta-cells function through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose. |
| Protein Length | 434 |
Protein Sequence
(FASTA) | | MNDRDLQMLK GEKLQVLKGT GDWWLARSLV TGREGYVPSN FVARVESLEM ERWFFRSQGR 60 | | KEAERQLLAP INKAGSFLIR ESETNKGAFS LSVKDVTTQG ELIKHYKIRC LDEGGYYISP 120 | | RITFPSLQAL VQHYSKKGDG LCQRLTLPCV RPAPQNPWAQ DEWEIPRQSL RLVRKLGSGQ 180 | | FGEVWMGYYK NNMKVAIKTL KEGTMSPEAF LGEANVMKAL QHERLVRLYA VVTKEPIYIV 240 | | TEYMARGCLL DFLKTDEGSR LSLPRLIDMS AQIAEGMAYI ERMNSIHRDL RAANILVSEA 300 | | LCCKIADFGL ARIIDSEYTA QEGAKFPIKW TAPEAIHFGV FTIKADVWSF GVLLMEVVTY 360 | | GRVPYPGMSN PEVIRNLERG YRMPRPDTCP PELYRGVIAE CWRSRPEERP TFEFLQSVLE 420 | | DFYTATERQY ELQP 434 |
|
Nucleotide Sequence
(FASTA) | | ATGAATGATC GGGACCTGCA GATGCTGAAG GGGGAGAAGC TACAGGTCCT GAAGGGAACT 60 | | GGAGACTGGT GGCTGGCCAG GTCACTCGTC ACAGGAAGAG AAGGCTATGT GCCCAGTAAC 120 | | TTTGTGGCCC GAGTGGAGAG CCTGGAAATG GAAAGGTGGT TCTTTAGATC ACAGGGTCGG 180 | | AAGGAGGCTG AGAGGCAGCT TCTTGCTCCA ATCAACAAGG CCGGCTCCTT TCTTATCAGA 240 | | GAGAGTGAAA CCAACAAAGG TGCCTTCTCC CTGTCTGTGA AGGATGTCAC CACCCAGGGG 300 | | GAGCTGATCA AGCACTATAA GATCCGCTGC CTGGATGAAG GGGGCTACTA CATCTCCCCC 360 | | CGGATCACCT TCCCCTCGCT CCAGGCCCTG GTGCAGCACT ATTCTAAGAA GGGGGATGGT 420 | | CTATGCCAGA GGCTGACCCT GCCCTGTGTG CGCCCGGCCC CGCAGAATCC CTGGGCCCAG 480 | | GATGAATGGG AGATCCCCCG GCAGTCTCTC AGGCTGGTCA GGAAACTCGG GTCTGGACAA 540 | | TTCGGCGAAG TCTGGATGGG TTACTACAAA AACAACATGA AGGTGGCCAT TAAGACGCTG 600 | | AAGGAGGGAA CCATGTCTCC AGAAGCCTTT CTGGGTGAGG CCAACGTGAT GAAGGCTCTG 660 | | CAGCACGAGC GGCTGGTCCG ACTCTACGCA GTGGTCACCA AGGAGCCCAT CTACATTGTC 720 | | ACCGAGTACA TGGCCAGAGG ATGCCTGCTG GATTTCCTGA AGACAGATGA AGGGAGCAGA 780 | | TTGTCACTCC CAAGGCTGAT TGACATGTCG GCGCAGATTG CTGAAGGGAT GGCATACATT 840 | | GAGCGCATGA ATTCCATCCA CCGCGACCTG CGGGCGGCCA ACATCCTGGT GTCTGAGGCC 900 | | TTGTGCTGCA AAATTGCTGA TTTTGGCTTG GCTCGAATCA TCGACAGTGA ATACACGGCC 960 | | CAAGAGGGGG CCAAGTTCCC CATCAAGTGG ACAGCCCCGG AAGCCATCCA CTTCGGGGTC 1020 | | TTCACCATCA AAGCAGACGT GTGGTCGTTT GGAGTCCTCC TGATGGAAGT TGTCACTTAT 1080 | | GGGCGGGTGC CATACCCAGG GATGAGCAAC CCCGAGGTCA TCCGCAACCT GGAGCGCGGC 1140 | | TACCGCATGC CGCGCCCCGA CACCTGCCCG CCCGAGCTGT ACCGCGGCGT CATCGCCGAG 1200 | | TGCTGGCGCA GCCGGCCCGA GGAGCGGCCC ACCTTCGAGT TCCTGCAGTC GGTGCTGGAG 1260 | | GACTTCTACA CGGCCACCGA GCGGCAGTAC GAGCTGCAGC CCTAG 1305 |
|
| Domain Profile | S: 1 lklvkklGeGqfGevwlgkwkgsvkvavktlkegtlspeafleeaqilkklrheklvkly 60 | l+lv+klG+GqfGevw+g++k+++kva+ktlkegt+speafl ea+++k l+he+lv+ly | Q: 170 LRLVRKLGSGQFGEVWMGYYKNNMKVAIKTLKEGTMSPEAFLGEANVMKALQHERLVRLY 229 | 689********************************************************* |
| S: 61 avvseeePiyivtelmakGslldflkeeegkklklpklvdlaaqvaeGmayleeknlihr 120 | avv++ ePiyivte+ma+G+lldflk++eg++l+lp+l+d++aq+aeGmay+e++n ihr | Q: 230 AVVTK-EPIYIVTEYMARGCLLDFLKTDEGSRLSLPRLIDMSAQIAEGMAYIERMNSIHR 288 | ****9.****************************************************** |
| S: 121 dlaarnvlvgeslvvkvadfGlarlieddeytakegaklPikWtaPeaiklgkftiksdv 180 | dl+a+n+lv+e l++k+adfGlar+i d+eyta+egak+PikWtaPeai++g+ftik+dv | Q: 289 DLRAANILVSEALCCKIADFGLARII-DSEYTAQEGAKFPIKWTAPEAIHFGVFTIKADV 347 | **************************.899****************************** |
| S: 181 WsfGillteivtkGkvPypGmtneevleqvergyrlprpeecpeelye.lllecwkkdpe 239 | WsfG+ll+e+vt+G+vPypGm+n ev++++ergyr+prp++cp ely+ +++ecw+++pe | Q: 348 WSFGVLLMEVVTYGRVPYPGMSNPEVIRNLERGYRMPRPDTCPPELYRgVIAECWRSRPE 407 | ************************************************9*********** |
| S: 240 erPtfetlqevl 251 | erPtfe+lq+vl | Q: 408 ERPTFEFLQSVL 419 | *********986 |
|
Domain Sequence
(FASTA) | | LRLVRKLGSG QFGEVWMGYY KNNMKVAIKT LKEGTMSPEA FLGEANVMKA LQHERLVRLY 60 | | AVVTKEPIYI VTEYMARGCL LDFLKTDEGS RLSLPRLIDM SAQIAEGMAY IERMNSIHRD 120 | | LRAANILVSE ALCCKIADFG LARIIDSEYT AQEGAKFPIK WTAPEAIHFG VFTIKADVWS 180 | | FGVLLMEVVT YGRVPYPGMS NPEVIRNLER GYRMPRPDTC PPELYRGVIA ECWRSRPEER 240 | | PTFEFLQSVL 250 |
|
| Keyword | ATP-binding; Cell membrane; Complete proteome; Diabetes mellitus; Kinase; Lipoprotein; Membrane; Myristate; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; SH2 domain; SH3 domain; Transferase; Tyrosine-protein kinase; Ubl conjugation. |
| Sequence Source | Ensembl |
| Orthology | |
| Gene Ontology | |
| KEGG | |
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| Pfam | |
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| Prints | |
| Created Date | 20-Feb-2013 |