| Tag | Content |
|---|
| EKPD ID | EKS-HOS-00244 |
| Classification | | Group/Family | Score | E-Value | Start | End | Domain Length |
|---|
| AGC/PDK1 | 423.9 | 1.5E-126 | 82 | 342 | 261 |
|
| Status | Reviewed |
| Ensembl Protein | ENSP00000395357 |
| UniProt Accession | O15530; H0Y4Z0; Q59EH6; Q6FI20; Q8IV52; Q9BRD5; |
| Protein Name | 3-phosphoinositide-dependent protein kinase 1 |
| Protein Synonyms/Alias | hPDK1; |
| Gene Name | PDPK1 |
| Gene Synonyms/Alias | PDPK1; PDK1; |
| Ensembl Information | |
| Organism | Homo sapiens |
| Functional Description | Serine/threonine kinase which acts as a master kinase,phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptional activity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controls proliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca(2+) entry and Ca(2+)-activated K(+) channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses. Provides negative feedback inhibition to toll-like receptor-mediated NF- kappa-B activation in macrophages. Isoform 3 is catalytically inactive. |
| Protein Length | 454 |
Protein Sequence
(FASTA) | | MARTTSQLYD AVPIQSSVVL CSCPSPSMVR TQTESSTPPG IPGGSRQGPA MDGTAAEPRP 60 | | GAGSLQHAQP PPQPRKKRPE DFKFGKILGE GSFSTVVLAR ELATSREYAI KILEKRHIIK 120 | | ENKVPYVTRE RDVMSRLDHP FFVKLYFTFQ DDEKLYFGLS YAKNGELLKY IRKIGSFDET 180 | | CTRFYTAEIV SALEYLHGKG IIHRDLKPEN ILLNEDMHIQ ITDFGTAKVL SPESKQARAN 240 | | SFVGTAQYVS PELLTEKSAC KSSDLWALGC IIYQLVAGLP PFRAGNEYLI FQKIIKLEYD 300 | | FPEKFFPKAR DLVEKLLVLD ATKRLGCEEM EGYGPLKAHP FFESVTWENL HQQTPPKLTA 360 | | YLPAMSEDDE DCYGNYDNLL SQFGCMQVSS SSSSHSLSAS DTGLPQRSGS NIEQYIHDLD 420 | | SNSFELDLQF SEDEKRLLLE KQAGGNPCLT GRII 454 |
|
Nucleotide Sequence
(FASTA) | | ATGGCCAGGA CCACCAGCCA GCTGTATGAC GCCGTGCCCA TCCAGTCCAG CGTGGTGTTA 60 | | TGTTCCTGCC CATCCCCATC AATGGTGAGG ACCCAGACTG AGTCCAGCAC GCCCCCTGGC 120 | | ATTCCTGGTG GCAGCAGGCA GGGCCCCGCC ATGGACGGCA CTGCAGCCGA GCCTCGGCCC 180 | | GGCGCCGGCT CCCTGCAGCA TGCCCAGCCT CCGCCGCAGC CTCGGAAGAA GCGGCCTGAG 240 | | GACTTCAAGT TTGGGAAAAT CCTTGGGGAA GGCTCTTTTT CCACGGTTGT CCTGGCTCGA 300 | | GAACTGGCAA CCTCCAGAGA ATATGCGATT AAAATTCTGG AGAAGCGACA TATCATAAAA 360 | | GAGAACAAGG TCCCCTATGT AACCAGAGAG CGGGATGTCA TGTCGCGCCT GGATCACCCC 420 | | TTCTTTGTTA AGCTTTACTT CACATTTCAG GACGACGAGA AGCTGTATTT CGGCCTTAGT 480 | | TATGCCAAAA ATGGAGAACT ACTTAAATAT ATTCGCAAAA TCGGTTCATT CGATGAGACC 540 | | TGTACCCGAT TTTACACGGC TGAGATTGTG TCTGCTTTAG AGTACTTGCA CGGCAAGGGC 600 | | ATCATTCACA GGGACCTTAA ACCGGAAAAC ATTTTGTTAA ATGAAGATAT GCACATCCAG 660 | | ATCACAGATT TTGGAACAGC AAAAGTCTTA TCCCCAGAGA GCAAACAAGC CAGGGCCAAC 720 | | TCATTCGTGG GAACAGCGCA GTACGTTTCT CCAGAGCTGC TCACGGAGAA GTCCGCCTGT 780 | | AAGAGTTCAG ACCTTTGGGC TCTTGGATGC ATAATATACC AGCTTGTGGC AGGACTCCCA 840 | | CCATTCCGAG CTGGAAACGA GTATCTTATA TTTCAGAAGA TCATTAAGTT GGAATATGAC 900 | | TTTCCAGAAA AATTCTTCCC TAAGGCAAGA GACCTCGTGG AGAAACTTTT GGTTTTAGAT 960 | | GCCACAAAGC GGTTAGGCTG TGAGGAAATG GAAGGATACG GACCTCTTAA AGCACACCCG 1020 | | TTCTTCGAGT CCGTCACGTG GGAGAACCTG CACCAGCAGA CGCCTCCGAA GCTCACCGCT 1080 | | TACCTGCCGG CTATGTCGGA AGACGACGAG GACTGCTATG GCAATTATGA CAATCTCCTG 1140 | | AGCCAGTTTG GCTGCATGCA GGTGTCTTCG TCCTCCTCCT CACACTCCCT GTCAGCCTCC 1200 | | GACACGGGCC TGCCCCAGAG GTCAGGCAGC AACATAGAGC AGTACATTCA CGATCTGGAC 1260 | | TCGAACTCCT TTGAACTGGA CTTACAGTTT TCCGAAGATG AGAAGAGGTT GTTGTTGGAG 1320 | | AAGCAGGCTG GCGGAAACCC TTGCCTAACA GGACGTATTA TCTGA 1365 |
|
| Domain Profile | S: 1 fkfekelgeGsystvvlarekeskkeyaikvlekryiikekkvkyvtrekevlaklnehp 60 | fkf+k+lgeGs+stvvlare+++++eyaik+lekr+iike+kv+yvtre++v+++l +hp | Q: 82 FKFGKILGEGSFSTVVLARELATSREYAIKILEKRHIIKENKVPYVTRERDVMSRL-DHP 140 | 89******************************************************.9** |
| S: 61 givkLyatfqDeeslyfvleyakkGdllellkkvgsfdeactryyaaellsaleflhkkg 120 | ++vkLy+tfqD+e+lyf l+yak+G+ll++++k+gsfde+ctr+y+ae++sale+lh kg | Q: 141 FFVKLYFTFQDDEKLYFGLSYAKNGELLKYIRKIGSFDETCTRFYTAEIVSALEYLHGKG 200 | ************************************************************ |
| S: 121 iihrDlkpeniLldkdgkilitDfGsakvlepklkedskkeeeesarsksfvGtaeyvsP 180 | iihrDlkpeniLl++d++i+itDfG+akvl+p++k+ ar++sfvGta+yvsP | Q: 201 IIHRDLKPENILLNEDMHIQITDFGTAKVLSPESKQ---------ARANSFVGTAQYVSP 251 | ******************************999999.........9************** |
| S: 181 elLtdsevskssDlWalgcilyqllaGkpPfraeneylifkkilkleyafpegfpekakd 240 | elLt+++++kssDlWalgci+yql+aG+pPfra neylif+ki+kley+fpe+f++ka+d | Q: 252 ELLTEKSACKSSDLWALGCIIYQLVAGLPPFRAGNEYLIFQKIIKLEYDFPEKFFPKARD 311 | ************************************************************ |
| S: 241 lveklLvkdakerltlee......ikahkff 265 | lveklLv da++rl++ee +kah+ff | Q: 312 LVEKLLVLDATKRLGCEEmegygpLKAHPFF 342 | ******************************9 |
|
Domain Sequence
(FASTA) | | FKFGKILGEG SFSTVVLARE LATSREYAIK ILEKRHIIKE NKVPYVTRER DVMSRLDHPF 60 | | FVKLYFTFQD DEKLYFGLSY AKNGELLKYI RKIGSFDETC TRFYTAEIVS ALEYLHGKGI 120 | | IHRDLKPENI LLNEDMHIQI TDFGTAKVLS PESKQARANS FVGTAQYVSP ELLTEKSACK 180 | | SSDLWALGCI IYQLVAGLPP FRAGNEYLIF QKIIKLEYDF PEKFFPKARD LVEKLLVLDA 240 | | TKRLGCEEME GYGPLKAHPF F 261 |
|
| Keyword | 3D-structure; Acetylation; Activator; Alternative splicing; ATP-binding; Cell junction; Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Membrane; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transcription; Transcription regulation; Transferase; Ubl conjugation. |
| Sequence Source | Ensembl |
| Orthology | |
| Gene Ontology | |
| KEGG | |
| InterPros | |
| Pfam | |
| SMARTs | |
| Prosites | |
| Prints | |
| Created Date | 20-Feb-2013 |