EKS-HOS-00270

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00270

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/PLK	388.6	6.7E-116	12	265	254

Status

Reviewed

Ensembl Protein

ENSP00000421774

UniProt Accession

O00444; B2RAL0; B7Z837; B7Z8G7; Q8IYF0; Q96Q95; Q9UD84; Q9UDE2;

Protein Name

Serine/threonine-protein kinase PLK4

Protein Synonyms/Alias

Polo-like kinase 4; PLK-4; Serine/threonine-protein kinase 18; Serine/threonine-protein kinase Sak;

Gene Name

PLK4

Gene Synonyms/Alias

PLK4; SAK, STK18;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000142731	ENSP00000427554	ENST00000513090
ENSG00000142731	ENSP00000270861	ENST00000270861
ENSG00000142731	ENSP00000421774	ENST00000515069
ENSG00000142731	ENSP00000427568	ENST00000508113
ENSG00000142731	ENSP00000423412	ENST00000507249
ENSG00000142731	ENSP00000423582	ENST00000514379

Organism

Homo sapiens

Functional Description

Serine/threonine-protein kinase that plays a centralrole in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a posible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2.

Protein Length

892

Protein Sequence
(FASTA)

MATCIGEKIE DFKVGNLLGK GSFAGVYRAE SIHTGLEVAI KMIDKKAMYK AGMVQRVQNE 60

VKIHCQLKHP SILELYNYFE DSNYVYLVLE MCHNGEMNRY LKNRVKPFSE NEARHFMHQI 120

ITGMLYLHSH GILHRDLTLS NLLLTRNMNI KIADFGLATQ LKMPHEKHYT LCGTPNYISP 180

EIATRSAHGL ESDVWSLGCM FYTLLIGRPP FDTDTVKNTL NKVVLADYEM PSFLSIEAKD 240

LIHQLLRRNP ADRLSLSSVL DHPFMSRNSS TKSKDLGTVE DSIDSGHATI STAITASSST 300

SISGSLFDKR RLLIGQPLPN KMTVFPKNKS STDFSSSGDG NSFYTQWGNQ ETSNSGRGRV 360

IQDAEERPHS RYLRRAYSSD RSGTSNSQSQ AKTYTMERCH SAEMLSVSKR SGGGENEERY 420

SPTDNNANIF NFFKEKTSSS SGSFERPDNN QALSNHLCPG KTPFPFADPT PQTETVQQWF 480

GNLQINDPLS EQSKTRGMEP PWGYQNRTLR SITSPLVAHR LKPIRQKTKK AVVSILDSEE 540

VCVELVKEYA SQEYVKEVLQ ISSDGNTITI YYPNGGRGFP LADRPPSPTD NISRYSFDNL 600

PEKYWRKYQY ASRFVQLVRS KSPKITYFTR YAKCILMENS PGADFEVWFY DGVKIHKTED 660

FIQVIEKTGK SYTLKSESEV NSLKEEIKMY MDHANEGHRI CLALESIISE EERKTRSAPF 720

FPIIIGRKPG STSSPKALSP PPSVDSNYPT RERASFNRMV MHSAASPTQA PILNPSMVTN 780

EGLGLTTTAS GTDISSNSLK DCLPKSAQLL KSVFVKNVGW ATQLTSGAVW VQFNDGSQLV 840

VQAGVSSISY TSPNGQTTRY GENEKLPDYI KQKLQCLSSI LLMFSNPTPN FH 892

Nucleotide Sequence
(FASTA)

ATGGCGACCT GCATCGGGGA GAAGATCGAG GATTTTAAAG TTGGAAATCT GCTTGGTAAA 60

GGATCATTTG CTGGTGTCTA CAGAGCTGAG TCCATTCACA CTGGTTTGGA AGTTGCAATC 120

AAAATGATAG ATAAGAAAGC CATGTACAAA GCAGGAATGG TACAGAGAGT CCAAAATGAG 180

GTGAAAATAC ATTGCCAATT GAAACATCCT TCTATCTTGG AGCTTTATAA CTATTTTGAA 240

GATAGCAATT ATGTGTATCT GGTATTAGAA ATGTGCCATA ATGGAGAAAT GAACAGGTAT 300

CTAAAGAATA GAGTGAAACC CTTCTCAGAA AATGAAGCTC GACACTTCAT GCACCAGATC 360

ATCACAGGGA TGTTGTATCT TCATTCTCAT GGTATACTAC ACCGGGACCT CACACTTTCT 420

AACCTCCTAC TGACTCGTAA TATGAACATC AAGATTGCTG ATTTTGGGCT GGCAACTCAA 480

CTGAAAATGC CACATGAAAA GCACTATACA TTATGTGGAA CTCCTAACTA CATTTCACCA 540

GAAATTGCCA CTCGAAGTGC ACATGGCCTT GAATCTGATG TTTGGTCCCT GGGCTGTATG 600

TTTTATACAT TACTTATCGG GAGACCACCC TTCGACACTG ACACAGTCAA GAACACATTA 660

AATAAAGTAG TATTGGCAGA TTATGAAATG CCATCTTTTT TGTCAATAGA GGCCAAGGAC 720

CTTATTCACC AGTTACTTCG TAGAAATCCA GCAGATCGTT TAAGTCTGTC TTCAGTATTG 780

GACCATCCTT TTATGTCCCG AAATTCTTCA ACAAAAAGTA AAGATTTAGG AACTGTGGAA 840

GACTCAATTG ATAGTGGGCA TGCCACAATT TCTACTGCAA TTACAGCTTC TTCCAGTACC 900

AGTATAAGTG GTAGTTTATT TGACAAAAGA AGACTTTTGA TTGGTCAGCC ACTCCCAAAT 960

AAAATGACTG TATTTCCAAA GAATAAAAGT TCAACTGATT TTTCTTCTTC AGGAGATGGA 1020

AACAGTTTTT ATACTCAGTG GGGAAATCAA GAAACCAGTA ATAGTGGAAG GGGAAGAGTA 1080

ATTCAAGATG CAGAAGAAAG GCCACATTCT CGATACCTTC GTAGAGCTTA TTCCTCTGAT 1140

AGATCTGGCA CTTCTAATAG TCAGTCTCAA GCAAAAACAT ATACAATGGA ACGATGTCAC 1200

TCAGCAGAAA TGCTTTCAGT GTCCAAAAGA TCAGGAGGAG GTGAAAATGA AGAGAGGTAC 1260

TCACCCACAG ACAACAATGC CAACATTTTT AACTTCTTTA AAGAAAAGAC ATCCAGTAGT 1320

TCTGGATCTT TTGAAAGACC TGATAACAAT CAAGCACTCT CCAATCATCT TTGTCCAGGA 1380

AAAACTCCTT TTCCATTTGC AGACCCGACA CCTCAGACTG AAACCGTACA ACAGTGGTTT 1440

GGGAATCTGC AAATAAATGA TCCTCTTTCT GAACAGAGCA AGACTAGGGG TATGGAGCCA 1500

CCATGGGGTT ATCAGAATCG TACATTAAGA AGCATTACAT CTCCGTTGGT TGCTCACAGG 1560

TTAAAACCAA TCAGACAGAA AACCAAAAAG GCTGTGGTGA GCATACTTGA TTCAGAGGAG 1620

GTGTGTGTGG AGCTTGTAAA GGAGTATGCA TCTCAAGAAT ATGTGAAAGA AGTTCTTCAG 1680

ATATCTAGTG ATGGAAATAC GATCACTATT TATTATCCAA ATGGTGGTAG AGGTTTTCCT 1740

CTTGCTGATA GACCACCCTC ACCTACTGAC AACATCAGTA GGTACAGCTT TGACAATTTA 1800

CCAGAAAAAT ACTGGCGAAA ATATCAATAT GCTTCCAGGT TTGTACAGCT TGTAAGATCT 1860

AAATCTCCCA AAATCACTTA TTTTACAAGA TATGCTAAAT GCATTTTGAT GGAGAATTCT 1920

CCTGGTGCTG ATTTTGAGGT TTGGTTTTAT GATGGGGTAA AAATACACAA AACAGAAGAT 1980

TTCATTCAGG TGATTGAAAA GACAGGGAAG TCTTACACTT TAAAAAGTGA AAGTGAAGTT 2040

AATAGCTTGA AAGAGGAGAT AAAAATGTAT ATGGACCATG CTAATGAGGG TCATCGTATT 2100

TGTTTAGCAC TGGAATCCAT AATTTCAGAA GAGGAAAGGA AAACTAGGAG TGCTCCCTTT 2160

TTCCCAATAA TCATAGGAAG AAAACCTGGT AGTACTAGTT CACCTAAGGC CTTATCACCT 2220

CCTCCTTCTG TGGATTCAAA TTACCCAACG AGAGAGAGAG CATCTTTCAA CAGAATGGTC 2280

ATGCATAGTG CTGCTTCTCC AACACAGGCA CCAATCCTTA ATCCCTCTAT GGTTACAAAT 2340

GAAGGACTTG GTCTTACAAC TACAGCTTCT GGAACAGACA TCTCTTCTAA TAGTCTAAAA 2400

GATTGTCTTC CTAAATCAGC ACAACTTTTG AAATCTGTTT TTGTGAAAAA TGTTGGTTGG 2460

GCTACACAGT TAACTAGTGG AGCTGTGTGG GTTCAGTTTA ATGATGGGTC CCAGTTGGTT 2520

GTGCAGGCAG GAGTGTCTTC TATCAGTTAT ACCTCACCAA ATGGTCAAAC AACTAGGTAT 2580

GGAGAAAATG AAAAATTACC AGACTACATC AAACAGAAAT TACAGTGTCT GTCTTCCATC 2640

CTTTTGATGT TTTCTAATCC GACTCCTAAT TTTCATTGA 2679

Domain Profile

S: 1     yergkllGkGgfakcyelkdletkevfavkvvpksllakekqrekvekeieihrslkhkn  60

         +++g+llGkG+fa +y++++++t+ ++a+k+++k++++k+ + ++v++e++ih++lkh++

Q: 12    FKVGNLLGKGSFAGVYRAESIHTGLEVAIKMIDKKAMYKAGMVQRVQNEVKIHCQLKHPS  71

         6899********************************************************

S: 61    ivklyeffedkenvylvlelcsrrslaellkrr.kaltepevryylrqivsglkylhekr  119

         i++ly++fed+++vylvle+c++++++++lk+r k+++e+e+r++++qi++g++ylh++

Q: 72    ILELYNYFEDSNYVYLVLEMCHNGEMNRYLKNRvKPFSENEARHFMHQIITGMLYLHSHG  131

         *********************************9**************************

S: 120   ilhrdlklgnlflneelevkigdfGlatrlekeeerkktlcGtPnyiaPevlskkghsle  179

         ilhrdl+l+nl+l++++++ki+dfGlat+l++++e+++tlcGtPnyi+Pe++++++h+le

Q: 132   ILHRDLTLSNLLLTRNMNIKIADFGLATQLKMPHEKHYTLCGTPNYISPEIATRSAHGLE  191

         ************************************************************

S: 180   vdiWslGcilytllvGkPPfetkelketyekikkaeyslPsslskeaksliakllkkePe  239

         +d+WslGc++ytll+G+PPf+t+++k+t++k++ a+y++Ps ls eak+li++ll+++P+

Q: 192   SDVWSLGCMFYTLLIGRPPFDTDTVKNTLNKVVLADYEMPSFLSIEAKDLIHQLLRRNPA  251

         ************************************************************

S: 240   erpsleevlkdefl  253

         +r+sl++vl+++f+

Q: 252   DRLSLSSVLDHPFM  265

         ************98

Domain Sequence
(FASTA)

FKVGNLLGKG SFAGVYRAES IHTGLEVAIK MIDKKAMYKA GMVQRVQNEV KIHCQLKHPS 60

ILELYNYFED SNYVYLVLEM CHNGEMNRYL KNRVKPFSEN EARHFMHQII TGMLYLHSHG 120

ILHRDLTLSN LLLTRNMNIK IADFGLATQL KMPHEKHYTL CGTPNYISPE IATRSAHGLE 180

SDVWSLGCMF YTLLIGRPPF DTDTVKNTLN KVVLADYEMP SFLSIEAKDL IHQLLRRNPA 240

DRLSLSSVLD HPFM 254

Keyword

3D-structure; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005814	; C:centriole
GO:0032154	; C:cleavage furrow
GO:0005829	; C:cytosol
GO:0005730	; C:nucleolus
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0004713	; F:protein tyrosine kinase activity
GO:0007099	; P:centriole replication
GO:0000086	; P:G2/M transition of mitotic cell cycle
GO:0046601	; P:positive regulation of centriole replication
GO:0060707	; P:trophoblast giant cell differentiation

KEGG

hsa:10733

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000959	; POLO_box_duplicated_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008266	; Tyr_kinase_AS.

Pfam

PF00069	; Pkinase; 1.
PF00659	; POLO_box; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS50078	; POLO_BOX; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; FALSE_NEG.

Prints

Created Date

20-Feb-2013