EKS-HOS-00267

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00267

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/PLK	428.2	5.6E-128	82	334	253

Status

Reviewed

Ensembl Protein

ENSP00000274289

UniProt Accession

Q9NYY3; O60679; Q96CV7; Q9UE61;

Protein Name

Serine/threonine-protein kinase PLK2

Protein Synonyms/Alias

Polo-like kinase 2; PLK-2; hPlk2; Serine/threonine-protein kinase SNK; hSNK; Serum-inducible kinase;

Gene Name

PLK2

Gene Synonyms/Alias

PLK2; SNK;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000145632	ENSP00000274289	ENST00000274289

Organism

Homo sapiens

Functional Description

Tumor suppressor serine/threonine-protein kinaseinvolved in synaptic plasticity, centriole duplication and G1/S phase transition. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates CENPJ, NPM1, RAPGEF2, RASGRF1, SNCA, SIPA1L1 and SYNGAP1. Plays a key role in synaptic plasticity and memory by regulating the Ras and Rap protein signaling: required for overactivity-dependent spine remodeling by phosphorylating the Ras activator RASGRF1 and the Rap inhibitor SIPA1L1 leading to their degradation by the proteasome. Conversely, phosphorylates the Rap activator RAPGEF2 and the Ras inhibitor SYNGAP1, promoting their activity. Also regulates synaptic plasticity independently of kinase activity, via its interaction with NSF that disrupts the interaction between NSF and the GRIA2 subunit of AMPARs, leading to a rapid rundown of AMPAR-mediated current that occludes long term depression. Required for procentriole formation and centriole duplication by phosphorylating CENPJ and NPM1, respectively. Its induction by p53/TP53 suggests that it may participate in the mitotic checkpoint following stress.

Protein Length

685

Protein Sequence
(FASTA)

MELLRTITYQ PAASTKMCEQ ALGKGCGADS KKKRPPQPPE ESQPPQSQAQ VPPAAPHHHH 60

HHSHSGPEIS RIIVDPTTGK RYCRGKVLGK GGFAKCYEMT DLTNNKVYAA KIIPHSRVAK 120

PHQREKIDKE IELHRILHHK HVVQFYHYFE DKENIYILLE YCSRRSMAHI LKARKVLTEP 180

EVRYYLRQIV SGLKYLHEQE ILHRDLKLGN FFINEAMELK VGDFGLAARL EPLEHRRRTI 240

CGTPNYLSPE VLNKQGHGCE SDIWALGCVM YTMLLGRPPF ETTNLKETYR CIREARYTMP 300

SSLLAPAKHL IASMLSKNPE DRPSLDDIIR HDFFLQGFTP DRLSSSCCHT VPDFHLSSPA 360

KNFFKKAAAA LFGGKKDKAR YIDTHNRVSK EDEDIYKLRH DLKKTSITQQ PSKHRTDEEL 420

QPPTTTVARS GTPAVENKQQ IGDAIRMIVR GTLGSCSSSS ECLEDSTMGS VADTVARVLR 480

GCLENMPEAD CIPKEQLSTS FQWVTKWVDY SNKYGFGYQL SDHTVGVLFN NGAHMSLLPD 540

KKTVHYYAEL GQCSVFPATD APEQFISQVT VLKYFSHYME ENLMDGGDLP SVTDIRRPRL 600

YLLQWLKSDK ALMMLFNDGT FQVNFYHDHT KIIICSQNEE YLLTYINEDR ISTTFRLTTL 660

LMSGCSSELK NRMEYALNML LQRCN 685

Nucleotide Sequence
(FASTA)

ATGGAGCTTT TGCGGACTAT CACCTACCAG CCAGCCGCCA GCACCAAAAT GTGCGAGCAG 60

GCGCTGGGCA AGGGTTGCGG AGCGGACTCG AAGAAGAAGC GGCCGCCGCA GCCCCCCGAG 120

GAATCGCAGC CACCTCAGTC CCAGGCGCAA GTGCCCCCGG CGGCCCCTCA CCACCATCAC 180

CACCATTCGC ACTCGGGGCC GGAGATCTCG CGGATTATCG TCGACCCCAC GACTGGGAAG 240

CGCTACTGCC GGGGCAAAGT GCTGGGAAAG GGTGGCTTTG CAAAATGTTA CGAGATGACA 300

GATTTGACAA ATAACAAAGT CTACGCCGCA AAAATTATTC CTCACAGCAG AGTAGCTAAA 360

CCTCATCAAA GGGAAAAGAT TGACAAAGAA ATAGAGCTTC ACAGAATTCT TCATCATAAG 420

CATGTAGTGC AGTTTTACCA CTACTTCGAG GACAAAGAAA ACATTTACAT TCTCTTGGAA 480

TACTGCAGTA GAAGGTCAAT GGCTCATATT TTGAAAGCAA GAAAGGTGTT GACAGAGCCA 540

GAAGTTCGAT ACTACCTCAG GCAGATTGTG TCTGGACTGA AATACCTTCA TGAACAAGAA 600

ATCTTGCACA GAGATCTCAA ACTAGGGAAC TTTTTTATTA ATGAAGCCAT GGAACTAAAA 660

GTTGGGGACT TCGGTCTGGC AGCCAGGCTA GAACCCTTGG AACACAGAAG GAGAACGATA 720

TGTGGTACCC CAAATTATCT CTCTCCTGAA GTCCTCAACA AACAAGGACA TGGCTGTGAA 780

TCAGACATTT GGGCCCTGGG CTGTGTAATG TATACAATGT TACTAGGGAG GCCCCCATTT 840

GAAACTACAA ATCTCAAAGA AACTTATAGG TGCATAAGGG AAGCAAGGTA TACAATGCCG 900

TCCTCATTGC TGGCTCCTGC CAAGCACTTA ATTGCTAGTA TGTTGTCCAA AAACCCAGAG 960

GATCGTCCCA GTTTGGATGA CATCATTCGA CATGACTTTT TTTTGCAGGG CTTCACTCCG 1020

GACAGACTGT CTTCTAGCTG TTGTCATACA GTTCCAGATT TCCACTTATC AAGCCCAGCT 1080

AAGAATTTCT TTAAGAAAGC AGCTGCTGCT CTTTTTGGTG GCAAAAAAGA CAAAGCAAGA 1140

TATATTGACA CACATAATAG AGTGTCTAAA GAAGATGAAG ACATCTACAA GCTTAGGCAT 1200

GATTTGAAAA AGACTTCAAT AACTCAGCAA CCCAGCAAAC ACAGGACAGA TGAGGAGCTC 1260

CAGCCACCTA CCACCACAGT TGCCAGGTCT GGAACACCCG CAGTAGAAAA CAAGCAGCAG 1320

ATTGGGGATG CTATTCGGAT GATAGTCAGA GGGACTCTTG GCAGCTGTAG CAGCAGCAGT 1380

GAATGCCTTG AAGACAGTAC CATGGGAAGT GTTGCAGACA CAGTGGCAAG GGTTCTTCGG 1440

GGATGTCTGG AAAACATGCC GGAAGCTGAT TGCATTCCCA AAGAGCAGCT GAGCACATCA 1500

TTTCAGTGGG TCACCAAATG GGTTGATTAC TCTAACAAAT ATGGCTTTGG GTACCAGCTC 1560

TCAGACCACA CCGTCGGTGT CCTTTTCAAC AATGGTGCTC ACATGAGCCT CCTTCCAGAC 1620

AAAAAAACAG TTCACTATTA CGCAGAGCTT GGCCAATGCT CAGTTTTCCC AGCAACAGAT 1680

GCTCCTGAGC AATTTATTAG TCAAGTGACG GTGCTGAAAT ACTTTTCTCA TTACATGGAG 1740

GAGAACCTCA TGGATGGTGG AGATCTGCCT AGTGTTACTG ATATTCGAAG ACCTCGGCTC 1800

TACCTCCTTC AGTGGCTAAA ATCTGATAAG GCCCTAATGA TGCTCTTTAA TGATGGCACC 1860

TTTCAGGTGA ATTTCTACCA TGATCATACA AAAATCATCA TCTGTAGCCA AAATGAAGAA 1920

TACCTTCTCA CCTACATCAA TGAGGATAGG ATATCTACAA CTTTCAGGCT GACAACTCTG 1980

CTGATGTCTG GCTGTTCATC AGAATTAAAA AATCGAATGG AATATGCCCT GAACATGCTC 2040

TTACAAAGAT GTAACTGA 2058

Domain Profile

S: 1     yergkllGkGgfakcyelkdletkevfavkvvpksllakekqrekvekeieihrslkhkn  60

         y+rgk+lGkGgfakcye++dl++++v+a+k++p+s++ak++qrek++keie+hr l+hk+

Q: 82    YCRGKVLGKGGFAKCYEMTDLTNNKVYAAKIIPHSRVAKPHQREKIDKEIELHRILHHKH  141

         99**********************************************************

S: 61    ivklyeffedkenvylvlelcsrrslaellkrrkaltepevryylrqivsglkylhekri  120

         +v++y++fedken+y++le+csrrs+a++lk+rk+ltepevryylrqivsglkylhe++i

Q: 142   VVQFYHYFEDKENIYILLEYCSRRSMAHILKARKVLTEPEVRYYLRQIVSGLKYLHEQEI  201

         ************************************************************

S: 121   lhrdlklgnlflneelevkigdfGlatrlekeeerkktlcGtPnyiaPevlskkghslev  180

         lhrdlklgn+f+ne++e+k+gdfGla+rle+ e+r++t+cGtPny++Pevl+k+gh++e+

Q: 202   LHRDLKLGNFFINEAMELKVGDFGLAARLEPLEHRRRTICGTPNYLSPEVLNKQGHGCES  261

         ************************************************************

S: 181   diWslGcilytllvGkPPfetkelketyekikkaeyslPsslskeaksliakllkkePee  240

         diW+lGc++yt+l+G+PPfet++lkety++i++a+y++Pssl ++ak+lia++l+k+Pe+

Q: 262   DIWALGCVMYTMLLGRPPFETTNLKETYRCIREARYTMPSSLLAPAKHLIASMLSKNPED  321

         ************************************************************

S: 241   rpsleevlkdefl  253

         rpsl+++++++f+

Q: 322   RPSLDDIIRHDFF  334

         ************8

Domain Sequence
(FASTA)

YCRGKVLGKG GFAKCYEMTD LTNNKVYAAK IIPHSRVAKP HQREKIDKEI ELHRILHHKH 60

VVQFYHYFED KENIYILLEY CSRRSMAHIL KARKVLTEPE VRYYLRQIVS GLKYLHEQEI 120

LHRDLKLGNF FINEAMELKV GDFGLAARLE PLEHRRRTIC GTPNYLSPEV LNKQGHGCES 180

DIWALGCVMY TMLLGRPPFE TTNLKETYRC IREARYTMPS SLLAPAKHLI ASMLSKNPED 240

RPSLDDIIRH DFF 253

Keyword

ATP-binding; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase; Tumor suppressor.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Choloepus hoffmanni"; ?>Danio rerio"; ?>Dasypus novemcinctus"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Rattus norvegicus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005814	; C:centriole
GO:0030425	; C:dendrite
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0004871	; F:signal transducer activity
GO:0000082	; P:G1/S transition of mitotic cell cycle
GO:0060292	; P:long term synaptic depression
GO:0060291	; P:long-term synaptic potentiation
GO:0007613	; P:memory
GO:0007093	; P:mitotic cell cycle checkpoint
GO:0007052	; P:mitotic spindle organization
GO:0043066	; P:negative regulation of apoptotic process
GO:0043123	; P:positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0032486	; P:Rap protein signal transduction
GO:0046599	; P:regulation of centriole replication

KEGG

hsa:10769

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000959	; POLO_box_duplicated_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069	; Pkinase; 1.
PF00659	; POLO_box; 2.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS50078	; POLO_BOX; 2.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013