EKS-HOS-00163

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00163

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/TLK	624.3	1.1E-187	291	570	280

Status

Reviewed

Ensembl Protein

ENSP00000463595

UniProt Accession

Q86UE8; D3DU07; Q9UKI7; Q9Y4F7;

Protein Name

Serine/threonine-protein kinase tousled-like 2

Protein Synonyms/Alias

HsHPK; PKU-alpha; Tousled-like kinase 2;

Gene Name

TLK2

Gene Synonyms/Alias

TLK2;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000146872	ENSP00000462637	ENST00000582195
ENSG00000146872	ENSP00000463986	ENST00000583690
ENSG00000146872	ENSP00000275780	ENST00000346027
ENSG00000146872	ENSP00000462036	ENST00000581041
ENSG00000146872	ENSP00000340800	ENST00000343388
ENSG00000146872	ENSP00000464516	ENST00000580705
ENSG00000146872	ENSP00000316512	ENST00000326270
ENSG00000146872	ENSP00000464557	ENST00000579450
ENSG00000146872	ENSP00000463814	ENST00000583843
ENSG00000146872	ENSP00000442311	ENST00000542523
ENSG00000146872	ENSP00000462851	ENST00000578697
ENSG00000146872	ENSP00000463595	ENST00000582809

Organism

Homo sapiens

Functional Description

Serine/threonine-protein kinase involved in the processof chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation. Phosphorylates the chromatin assembly factors ASF1A AND ASF1B. Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly. Negative regulator of amino acid starvation-induced autophagy.

Protein Length

601

Protein Sequence
(FASTA)

MSVMLAKPRL DTEQLAQRGA GLCFTFVSAQ QNSPSSTGSG NTEHSCSSQK QISIQHRQTQ 60

SDLTIEKISA LENSKNSDLE KKEGRIDDLL RANCDLRRQI DEQQKMLEKY KERLNRCVTM 120

SKKLLIEKSK QEKMACRDKS MQDRLRLGHF TTVRHGASFT EQWTDGYAFQ NLIKQQERIN 180

SQREEIERQR KMLAKRKPPA MGQAPPATNE QKQRKSKTNG AENETLTLAE YHEQEEIFKL 240

RLGHLKKEEA EIQAELERLE RVRNLHIREL KRIHNEDNSQ FKDHPTLNDR YLLLHLLGRG 300

GFSEVYKAFD LTEQRYVAVK IHQLNKNWRD EKKENYHKHA CREYRIHKEL DHPRIVKLYD 360

YFSLDTDSFC TVLEYCEGND LDFYLKQHKL MSEKEARSII MQIVNALKYL NEIKPPIIHY 420

DLKPGNILLV NGTACGEIKI TDFGLSKIMD DDSYNSVDGM ELTSQGAGTY WYLPPECFVV 480

GKEPPKISNK VDVWSVGVIF YQCLYGRKPF GHNQSQQDIL QENTILKATE VQFPPKPVVT 540

PEAKAFIRRC LAYRKEDRID VQQLACDPYL LPHIRKSVST SSPAGAAIAS TSGASNNSSS 600

N 601

Nucleotide Sequence
(FASTA)

ATGTCAGTGA TGCTAGCAAA ACCTCGGCTT GACACAGAGC AGCTGGCGCA AAGGGGAGCT 60

GGCCTCTGCT TCACTTTTGT TTCAGCTCAG CAAAACAGTC CCTCATCTAC GGGATCTGGC 120

AACACAGAGC ATTCCTGCAG CTCCCAAAAA CAGATCTCCA TCCAGCACAG ACAGACCCAG 180

TCCGACCTCA CAATAGAAAA AATATCTGCA CTAGAAAACA GTAAGAATTC TGACTTAGAG 240

AAGAAGGAGG GAAGAATAGA TGATTTATTA AGAGCCAACT GTGATTTGAG ACGGCAGATT 300

GATGAACAGC AAAAGATGCT AGAGAAATAC AAGGAACGAT TAAATAGATG TGTGACAATG 360

AGCAAGAAAC TCCTTATAGA AAAGTCAAAA CAAGAGAAGA TGGCGTGTAG AGATAAGAGC 420

ATGCAAGACC GCTTGAGACT GGGCCACTTT ACTACTGTCC GACACGGAGC CTCATTTACT 480

GAACAGTGGA CAGATGGTTA TGCTTTTCAG AATCTTATCA AGCAACAGGA AAGGATAAAT 540

TCACAGAGGG AAGAGATAGA AAGACAACGG AAAATGTTAG CAAAGCGGAA ACCTCCTGCC 600

ATGGGTCAGG CCCCTCCTGC AACCAATGAG CAGAAACAGC GGAAAAGCAA GACCAATGGA 660

GCTGAAAATG AAACGTTAAC GTTAGCAGAA TACCATGAAC AAGAAGAAAT CTTCAAACTC 720

AGATTAGGTC ATCTTAAAAA GGAGGAAGCA GAGATCCAGG CAGAGCTGGA GAGACTAGAA 780

AGGGTTAGAA ATCTACATAT CAGGGAACTA AAAAGGATAC ATAATGAAGA TAATTCACAA 840

TTTAAAGATC ATCCAACGCT AAATGACAGA TATTTGTTGT TACATCTTTT GGGTAGAGGA 900

GGTTTCAGTG AAGTTTACAA GGCATTTGAT CTAACAGAGC AAAGATACGT AGCTGTGAAA 960

ATTCACCAGT TAAATAAAAA CTGGAGAGAT GAGAAAAAGG AGAATTACCA CAAGCATGCA 1020

TGTAGGGAAT ACCGGATTCA TAAAGAGCTG GATCATCCCA GAATAGTTAA GCTGTATGAT 1080

TACTTTTCAC TGGATACTGA CTCGTTTTGT ACAGTATTAG AATACTGTGA GGGAAATGAT 1140

CTGGACTTCT ACCTGAAACA GCACAAATTA ATGTCGGAGA AAGAGGCCCG GTCCATTATC 1200

ATGCAGATTG TGAATGCTTT AAAGTACTTA AATGAAATAA AACCTCCCAT CATACACTAT 1260

GACCTCAAAC CAGGTAATAT TCTTTTAGTA AATGGTACAG CGTGTGGAGA GATAAAAATT 1320

ACAGATTTTG GTCTTTCGAA GATCATGGAT GATGATAGCT ACAATTCAGT GGATGGCATG 1380

GAGCTAACAT CACAAGGTGC TGGTACTTAT TGGTATTTAC CACCAGAGTG TTTTGTGGTT 1440

GGGAAAGAAC CACCAAAGAT CTCAAATAAA GTTGATGTGT GGTCGGTGGG TGTGATCTTC 1500

TATCAGTGTC TTTATGGAAG GAAGCCTTTT GGCCATAACC AGTCTCAGCA AGACATCCTA 1560

CAAGAGAATA CGATTCTTAA AGCTACTGAA GTGCAGTTCC CGCCAAAGCC AGTAGTAACA 1620

CCTGAAGCAA AGGCGTTTAT TCGACGATGC TTGGCCTACC GAAAGGAGGA CCGCATTGAT 1680

GTCCAGCAGC TGGCCTGTGA TCCCTACTTG TTGCCTCACA TCCGAAAGTC AGTCTCTACA 1740

AGTAGCCCTG CTGGAGCTGC TATTGCATCA ACCTCTGGGG CGTCCAATAA CAGTTCTTCT 1800

AATTGA 1806

Domain Profile

S: 1     ylllkllgkggfsevykafdlkeqryvavkihqlnkdwkdekkenyvkhalreyrihkel  60

         ylll+llg+ggfsevykafdl+eqryvavkihqlnk+w+dekkeny+kha+reyrihkel

Q: 291   YLLLHLLGRGGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHACREYRIHKEL  350

         9***********************************************************

S: 61    dhprivklydllsldtdsfctvleyvegndldfylkqhkllsekearsiimqivsalkyl  120

         dhprivklyd++sldtdsfctvley+egndldfylkqhkl+sekearsiimqiv+alkyl

Q: 351   DHPRIVKLYDYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIIMQIVNALKYL  410

         ************************************************************

S: 121   neikppiihydlkpgnillvegtacgeikitdfglskimdddsydsddgleltsqgagty  180

         neikppiihydlkpgnillv+gtacgeikitdfglskimdddsy+s+dg+eltsqgagty

Q: 411   NEIKPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIMDDDSYNSVDGMELTSQGAGTY  470

         ************************************************************

S: 181   wylppecfvvgkeppkisnkvdvwsvgvifyqclygkkpfghnqsqqdileentilkate  240

         wylppecfvvgkeppkisnkvdvwsvgvifyqclyg+kpfghnqsqqdil+entilkate

Q: 471   WYLPPECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILKATE  530

         ************************************************************

S: 241   vqfpskpvvsseakafirrclayrkedridvqqlakdeyl  280

         vqfp+kpvv++eakafirrclayrkedridvqqla+d+yl

Q: 531   VQFPPKPVVTPEAKAFIRRCLAYRKEDRIDVQQLACDPYL  570

         **************************************97

Domain Sequence
(FASTA)

YLLLHLLGRG GFSEVYKAFD LTEQRYVAVK IHQLNKNWRD EKKENYHKHA CREYRIHKEL 60

DHPRIVKLYD YFSLDTDSFC TVLEYCEGND LDFYLKQHKL MSEKEARSII MQIVNALKYL 120

NEIKPPIIHY DLKPGNILLV NGTACGEIKI TDFGLSKIMD DDSYNSVDGM ELTSQGAGTY 180

WYLPPECFVV GKEPPKISNK VDVWSVGVIF YQCLYGRKPF GHNQSQQDIL QENTILKATE 240

VQFPPKPVVT PEAKAFIRRC LAYRKEDRID VQQLACDPYL 280

Keyword

Alternative splicing; ATP-binding; Cell cycle; Chromatin regulator; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; DNA damage; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005882	; C:intermediate filament
GO:0005634	; C:nucleus
GO:0048471	; C:perinuclear region of cytoplasm
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0007049	; P:cell cycle
GO:0071480	; P:cellular response to gamma radiation
GO:0016568	; P:chromatin modification
GO:0007059	; P:chromosome segregation
GO:0035556	; P:intracellular signal transduction
GO:0010507	; P:negative regulation of autophagy
GO:0032435	; P:negative regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0018105	; P:peptidyl-serine phosphorylation
GO:0001672	; P:regulation of chromatin assembly or disassembly
GO:0006974	; P:response to DNA damage stimulus

KEGG

hsa:11011

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013