EKS-HOS-00012

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00012

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/Eph	434.4	5.1E-130	625	879	255

Status

Reviewed

Ensembl Protein

ENSP00000275815

UniProt Accession

P21709; A1L3V3; Q15405;

Protein Name

Ephrin type-A receptor 1

Protein Synonyms/Alias

hEpha1; EPH tyrosine kinase; EPH tyrosine kinase 1; Erythropoietin-producing hepatoma receptor; Tyrosine-protein kinase receptor EPH;

Gene Name

EPHA1

Gene Synonyms/Alias

EPHA1; EPH, EPHT, EPHT1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000146904	ENSP00000275815	ENST00000275815

Organism

Homo sapiens

Functional Description

Receptor tyrosine kinase which binds promiscuouslymembrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis.

Protein Length

976

Protein Sequence
(FASTA)

MERRWPLGLG LVLLLCAPLP PGARAKEVTL MDTSKAQGEL GWLLDPPKDG WSEQQQILNG 60

TPLYMYQDCP MQGRRDTDHW LRSNWIYRGE EASRVHVELQ FTVRDCKSFP GGAGPLGCKE 120

TFNLLYMESD QDVGIQLRRP LFQKVTTVAA DQSFTIRDLV SGSVKLNVER CSLGRLTRRG 180

LYLAFHNPGA CVALVSVRVF YQRCPETLNG LAQFPDTLPG PAGLVEVAGT CLPHARASPR 240

PSGAPRMHCS PDGEWLVPVG RCHCEPGYEE GGSGEACVAC PSGSYRMDMD TPHCLTCPQQ 300

STAESEGATI CTCESGHYRA PGEGPQVACT GPPSAPRNLS FSASGTQLSL RWEPPADTGG 360

RQDVRYSVRC SQCQGTAQDG GPCQPCGVGV HFSPGARGLT TPAVHVNGLE PYANYTFNVE 420

AQNGVSGLGS SGHASTSVSI SMGHAESLSG LSLRLVKKEP RQLELTWAGS RPRSPGANLT 480

YELHVLNQDE ERYQMVLEPR VLLTELQPDT TYIVRVRMLT PLGPGPFSPD HEFRTSPPVS 540

RGLTGGEIVA VIFGLLLGAA LLLGILVFRS RRAQRQRQQR QRDRATDVDR EDKLWLKPYV 600

DLQAYEDPAQ GALDFTRELD PAWLMVDTVI GEGEFGEVYR GTLRLPSQDC KTVAIKTLKD 660

TSPGGQWWNF LREATIMGQF SHPHILHLEG VVTKRKPIMI ITEFMENGAL DAFLREREDQ 720

LVPGQLVAML QGIASGMNYL SNHNYVHRDL AARNILVNQN LCCKVSDFGL TRLLDDFDGT 780

YETQGGKIPI RWTAPEAIAH RIFTTASDVW SFGIVMWEVL SFGDKPYGEM SNQEVMKSIE 840

DGYRLPPPVD CPAPLYELMK NCWAYDRARR PHFQKLQAHL EQLLANPHSL RTIANFDPRM 900

TLRLPSLSGS DGIPYRTVSE WLESIRMKRY ILHFHSAGLD TMECVLELTA EDLTQMGITL 960

PGHQKRILCS IQGFKD 976

Nucleotide Sequence
(FASTA)

ATGGAGCGGC GCTGGCCCCT GGGGCTAGGG CTGGTGCTGC TGCTCTGCGC CCCGCTGCCC 60

CCGGGGGCGC GCGCCAAGGA AGTTACTCTG ATGGACACAA GCAAGGCACA GGGAGAGCTG 120

GGCTGGCTGC TGGATCCCCC AAAAGATGGG TGGAGTGAAC AGCAACAGAT ACTGAATGGG 180

ACACCCCTGT ACATGTACCA GGACTGCCCA ATGCAAGGAC GCAGAGACAC TGACCACTGG 240

CTTCGCTCCA ATTGGATCTA CCGCGGGGAG GAGGCTTCCC GCGTCCACGT GGAGCTGCAG 300

TTCACCGTGC GGGACTGCAA GAGTTTCCCT GGGGGAGCCG GGCCTCTGGG CTGCAAGGAG 360

ACCTTCAACC TTCTGTACAT GGAGAGTGAC CAGGATGTGG GCATTCAGCT CCGACGGCCC 420

TTGTTCCAGA AGGTAACCAC GGTGGCTGCA GACCAGAGCT TCACCATTCG AGACCTTGTG 480

TCTGGCTCCG TGAAGCTGAA TGTGGAGCGC TGCTCTCTGG GCCGCCTGAC CCGCCGTGGC 540

CTCTACCTCG CTTTCCACAA CCCGGGTGCC TGTGTGGCCC TGGTGTCTGT CCGGGTCTTC 600

TACCAGCGCT GTCCTGAGAC CCTGAATGGC TTGGCCCAAT TCCCAGACAC TCTGCCTGGC 660

CCCGCTGGGT TGGTGGAAGT GGCGGGGACC TGCTTGCCCC ACGCGCGGGC CAGCCCCAGG 720

CCCTCAGGTG CACCCCGCAT GCACTGCAGC CCTGATGGCG AGTGGCTGGT GCCTGTAGGA 780

CGGTGCCACT GTGAGCCTGG CTATGAGGAA GGTGGCAGTG GCGAAGCATG TGTTGCCTGC 840

CCTAGCGGCT CCTACCGGAT GGACATGGAC ACACCCCATT GTCTCACGTG CCCCCAGCAG 900

AGCACTGCTG AGTCTGAGGG GGCCACCATC TGTACCTGTG AGAGCGGCCA TTACAGAGCT 960

CCCGGGGAGG GCCCCCAGGT GGCATGCACA GGTCCCCCCT CGGCCCCCCG AAACCTGAGC 1020

TTCTCTGCCT CAGGGACTCA GCTCTCCCTG CGTTGGGAAC CCCCAGCAGA TACGGGGGGA 1080

CGCCAGGATG TCAGATACAG TGTGAGGTGT TCCCAGTGTC AGGGCACAGC ACAGGACGGG 1140

GGGCCCTGCC AGCCCTGTGG GGTGGGCGTG CACTTCTCGC CGGGGGCCCG GGGGCTCACC 1200

ACACCTGCAG TGCATGTCAA TGGCCTTGAA CCTTATGCCA ACTACACCTT TAATGTGGAA 1260

GCCCAAAATG GAGTGTCAGG GCTGGGCAGC TCTGGCCATG CCAGCACCTC AGTCAGCATC 1320

AGCATGGGGC ATGCAGAGTC ACTGTCAGGC CTGTCTCTGA GACTGGTGAA GAAAGAACCG 1380

AGGCAACTAG AGCTGACCTG GGCGGGGTCC CGGCCCCGAA GCCCTGGGGC GAACCTGACC 1440

TATGAGCTGC ACGTGCTGAA CCAGGATGAA GAACGGTACC AGATGGTTCT AGAACCCAGG 1500

GTCTTGCTGA CAGAGCTGCA GCCTGACACC ACATACATCG TCAGAGTCCG AATGCTGACC 1560

CCACTGGGTC CTGGCCCTTT CTCCCCTGAT CATGAGTTTC GGACCAGCCC ACCAGTGTCC 1620

AGGGGCCTGA CTGGAGGAGA GATTGTAGCC GTCATCTTTG GGCTGCTGCT TGGTGCAGCC 1680

TTGCTGCTTG GGATTCTCGT TTTCCGGTCC AGGAGAGCCC AGCGGCAGAG GCAGCAGAGG 1740

CAGCGTGACC GCGCCACCGA TGTGGATCGA GAGGACAAGC TGTGGCTGAA GCCTTATGTG 1800

GACCTCCAGG CATACGAGGA CCCTGCACAG GGAGCCCTGG ACTTTACCCG GGAGCTTGAT 1860

CCAGCGTGGC TGATGGTGGA CACTGTCATA GGAGAAGGAG AGTTTGGGGA AGTGTATCGA 1920

GGGACCCTGA GGCTCCCCAG CCAGGACTGC AAGACTGTGG CCATTAAGAC CTTAAAAGAC 1980

ACATCCCCAG GTGGCCAGTG GTGGAACTTC CTTCGAGAGG CAACTATCAT GGGCCAGTTT 2040

AGCCACCCGC ATATTCTGCA TCTGGAAGGC GTCGTCACAA AGCGAAAGCC GATCATGATC 2100

ATCACAGAAT TTATGGAGAA TGGAGCCCTG GATGCCTTCC TGAGGGAGCG GGAGGACCAG 2160

CTGGTCCCTG GGCAGCTAGT GGCCATGCTG CAGGGCATAG CATCTGGCAT GAACTACCTC 2220

AGTAATCACA ATTATGTCCA CCGGGACCTG GCTGCCAGAA ACATCTTGGT GAATCAAAAC 2280

CTGTGCTGCA AGGTGTCTGA CTTTGGCCTG ACTCGCCTCC TGGATGACTT TGATGGCACA 2340

TACGAAACCC AGGGAGGAAA GATCCCTATC CGTTGGACAG CCCCTGAAGC CATTGCCCAT 2400

CGGATCTTCA CCACAGCCAG CGATGTGTGG AGCTTTGGGA TTGTGATGTG GGAGGTGCTG 2460

AGCTTTGGGG ACAAGCCTTA TGGGGAGATG AGCAATCAGG AGGTTATGAA GAGCATTGAG 2520

GATGGGTACC GGTTGCCCCC TCCTGTGGAC TGCCCTGCCC CTCTGTATGA GCTCATGAAG 2580

AACTGCTGGG CATATGACCG TGCCCGCCGG CCACACTTCC AGAAGCTTCA GGCACATCTG 2640

GAGCAACTGC TTGCCAACCC CCACTCCCTG CGGACCATTG CCAACTTTGA CCCCAGGATG 2700

ACTCTTCGCC TGCCCAGCCT GAGTGGCTCA GATGGGATCC CATATCGAAC CGTCTCTGAG 2760

TGGCTCGAGT CCATACGCAT GAAACGCTAC ATCCTGCACT TCCACTCGGC TGGGCTGGAC 2820

ACCATGGAGT GTGTGCTGGA GCTGACCGCT GAGGACCTGA CGCAGATGGG AATCACACTG 2880

CCCGGGCACC AGAAGCGCAT TCTTTGCAGT ATTCAGGGAT TCAAGGACTG A 2931

Domain Profile

S: 2     kiekviGaGefGevcsGrlklpgkreiavaiktlkagstekqrrdflseasilGqfdhpn  61

         ++++viG+GefGev++G+l+lp++   +vaiktlk++s   q+++fl+ea+i+Gqf+hp+

Q: 625   MVDTVIGEGEFGEVYRGTLRLPSQDCKTVAIKTLKDTSPGGQWWNFLREATIMGQFSHPH  684

         6899******************999999********************************

S: 62    iirleGvvtkskpvmivteymengaldsflrkkdgqftviqlvgmlrGiasgmkylsdmg  121

         i++leGvvtk+kp+mi+te+mengald+flr++++q++  qlv+ml+Giasgm+yls+++

Q: 685   ILHLEGVVTKRKPIMIITEFMENGALDAFLREREDQLVPGQLVAMLQGIASGMNYLSNHN  744

         ************************************************************

S: 122   yvhrdlaarnilvnsnlvckvsdfGlsrvleddseaayttsggkipirwtapeaiayrkf  181

         yvhrdlaarnilvn+nl+ckvsdfGl+r l dd + +y+t+ggkipirwtapeaia+r f

Q: 745   YVHRDLAARNILVNQNLCCKVSDFGLTRLL-DDFDGTYETQGGKIPIRWTAPEAIAHRIF  803

         ****************************98.7889*************************

S: 182   tsasdvwsyGivlwevlsyGerpywdmsnqdvikaieegyrlpppmdcpaalyqlmldcw  241

         t+asdvws+Giv+wevls+G++py +msnq+v+k+ie+gyrlppp+dcpa ly+lm++cw

Q: 804   TTASDVWSFGIVMWEVLSFGDKPYGEMSNQEVMKSIEDGYRLPPPVDCPAPLYELMKNCW  863

         ************************************************************

S: 242   qkdraerpkfeqivsi  257

           dra+rp+f++++++

Q: 864   AYDRARRPHFQKLQAH  879

         ************9986

Domain Sequence
(FASTA)

MVDTVIGEGE FGEVYRGTLR LPSQDCKTVA IKTLKDTSPG GQWWNFLREA TIMGQFSHPH 60

ILHLEGVVTK RKPIMIITEF MENGALDAFL REREDQLVPG QLVAMLQGIA SGMNYLSNHN 120

YVHRDLAARN ILVNQNLCCK VSDFGLTRLL DDFDGTYETQ GGKIPIRWTA PEAIAHRIFT 180

TASDVWSFGI VMWEVLSFGD KPYGEMSNQE VMKSIEDGYR LPPPVDCPAP LYELMKNCWA 240

YDRARRPHFQ KLQAH 255

Keyword

3D-structure; Angiogenesis; ATP-binding; Cell adhesion; Cell membrane; Complete proteome; Glycoprotein; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Polymorphism; Receptor; Reference proteome; Repeat; Signal; Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>

Gene Ontology

GO:0005887	; C:integral to plasma membrane
GO:0005524	; F:ATP binding
GO:0005005	; F:transmembrane-ephrin receptor activity
GO:0032862	; P:activation of Rho GTPase activity
GO:0001525	; P:angiogenesis
GO:0030336	; P:negative regulation of cell migration
GO:0006469	; P:negative regulation of protein kinase activity
GO:0018108	; P:peptidyl-tyrosine phosphorylation
GO:0045766	; P:positive regulation of angiogenesis
GO:0030335	; P:positive regulation of cell migration
GO:0008284	; P:positive regulation of cell proliferation
GO:0001954	; P:positive regulation of cell-matrix adhesion
GO:0051496	; P:positive regulation of stress fiber assembly
GO:0046777	; P:protein autophosphorylation
GO:0032314	; P:regulation of Rac GTPase activity
GO:0034446	; P:substrate adhesion-dependent cell spreading

KEGG

hsa:2041

;

InterPros

IPR001090	; Ephrin_rcpt_lig-bd_dom.
IPR003961	; Fibronectin_type3.
IPR008979	; Galactose-bd-like.
IPR009030	; Growth_fac_rcpt.
IPR013783	; Ig-like_fold.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001660	; SAM.
IPR013761	; SAM/pointed.
IPR021129	; SAM_type1.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.
IPR016257	; Tyr_kinase_ephrin_rcpt.
IPR001426	; Tyr_kinase_rcpt_V_CS.

Pfam

PF01404	; Ephrin_lbd; 1.
PF00041	; fn3; 2.
PF07714	; Pkinase_Tyr; 1.
PF00536	; SAM_1; 1.

SMARTs

SM00615	; EPH_lbd; 1.
SM00060	; FN3; 2.
SM00454	; SAM; 1.
SM00219	; TyrKc; 1.

Prosites

PS01186	; EGF_2; UNKNOWN_1.
PS51550	; EPH_LBD; 1.
PS50853	; FN3; 2.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS00790	; RECEPTOR_TYR_KIN_V_1; 1.
PS00791	; RECEPTOR_TYR_KIN_V_2; 1.
PS50105	; SAM_DOMAIN; 1.

Prints

PR00109

; TYRKINASE.

Created Date

20-Feb-2013