EKS-HOS-00298

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00298

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/BUB	320.9	2.8E-95	753	1017	265

Status

Reviewed

Ensembl Protein

ENSP00000287598

UniProt Accession

O60566; B2R6U0; B4DL09; B4DLG3; O60501; O60627; O60758; O75389; Q59HH6; Q8WV50; Q96KM4;

Protein Name

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Protein Synonyms/Alias

MAD3/BUB1-related protein kinase; hBUBR1; Mitotic checkpoint kinase MAD3L; Protein SSK1;

Gene Name

BUB1B

Gene Synonyms/Alias

BUB1B; BUBR1, MAD3L, SSK1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000156970	ENSP00000398470	ENST00000412359
ENSG00000156970	ENSP00000453861	ENST00000558715
ENSG00000156970	ENSP00000287598	ENST00000287598
ENSG00000156970	ENSP00000453643	ENST00000559733

Organism

Homo sapiens

Functional Description

Essential component of the mitotic checkpoint. Requiredfor normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.

Protein Length

1050

Protein Sequence
(FASTA)

MAAVKKEGGA LSEAMSLEGD EWELSKENVQ PLRQGRIMST LQGALAQESA CNNTLQQQKR 60

AFEYEIRFYT GNDPLDVWDR YISWTEQNYP QGGKESNMST LLERAVEALQ GEKRYYSDPR 120

FLNLWLKLGR LCNEPLDMYS YLHNQGIGVS LAQFYISWAE EYEARENFRK ADAIFQEGIQ 180

QKAEPLERLQ SQHRQFQARV SRQTLLALEK EEEEEVFESS VPQRSTLAEL KSKGKKTARA 240

PIIRVGGALK APSQNRGLQN PFPQQMQNNS RITVFDENAD EASTAELSKP TVQPWIAPPM 300

PRAKENELQA GPWNTGRSLE HRPRGNTASL IAVPAVLPSF TPYVEETARQ PVMTPCKIEP 360

SINHILSTRK PGKEEGDPLQ RVQSHQQASE EKKEKMMYCK EKIYAGVGEF SFEEIRAEVF 420

RKKLKEQREA ELLTSAEKRA EMQKQIEEME KKLKEIQTTQ QERTGDQQEE TMPTKETTKL 480

QIASESQKIP GMTLSSSVCQ VNCCARETSL AENIWQEQPH SKGPSVPFSI FDEFLLSEKK 540

NKSPPADPPR VLAQRRPLAV LKTSESITSN EDVSPDVCDE FTGIEPLSED AIITGFRNVT 600

ICPNPEDTCD FARAARFVST PFHEIMSLKD LPSDPERLLP EEDLDVKTSE DQQTACGTIY 660

SQTLSIKKLS PIIEDSREAT HSSGFSGSSA SVASTSSIKC LQIPEKLELT NETSENPTQS 720

PWCSQYRRQL LKSLPELSAS AELCIEDRPM PKLEIEKEIE LGNEDYCIKR EYLICEDYKL 780

FWVAPRNSAE LTVIKVSSQP VPWDFYINLK LKERLNEDFD HFCSCYQYQD GCIVWHQYIN 840

CFTLQDLLQH SEYITHEITV LIIYNLLTIV EMLHKAEIVH GDLSPRCLIL RNRIHDPYDC 900

NKNNQALKIV DFSYSVDLRV QLDVFTLSGF RTVQILEGQK ILANCSSPYQ VDLFGIADLA 960

HLLLFKEHLQ VFWDGSFWKL SQNISELKDG ELWNKFFVRI LNANDEATVS VLGELAAEMN 1020

GVFDTTFQSH LNKALWKVGK LTSPGALLFQ 1050

Nucleotide Sequence
(FASTA)

ATGGCGGCGG TGAAGAAGGA AGGGGGTGCT CTGAGTGAAG CCATGTCCCT GGAGGGAGAT 60

GAATGGGAAC TGAGTAAAGA AAATGTACAA CCTTTAAGGC AAGGGCGGAT CATGTCCACG 120

CTTCAGGGAG CACTGGCACA AGAATCTGCC TGTAACAATA CTCTTCAGCA GCAGAAACGG 180

GCATTTGAAT ATGAAATTCG ATTTTACACT GGAAATGACC CTCTGGATGT TTGGGATAGG 240

TATATCAGCT GGACAGAGCA GAACTATCCT CAAGGTGGGA AGGAGAGTAA TATGTCAACG 300

TTATTAGAAA GAGCTGTAGA AGCACTACAA GGAGAAAAAC GATATTATAG TGATCCTCGA 360

TTTCTCAATC TCTGGCTTAA ATTAGGGCGT TTATGCAATG AGCCTTTGGA TATGTACAGT 420

TACTTGCACA ACCAAGGGAT TGGTGTTTCA CTTGCTCAGT TCTATATCTC ATGGGCAGAA 480

GAATATGAAG CTAGAGAAAA CTTTAGGAAA GCAGATGCGA TATTTCAGGA AGGGATTCAA 540

CAGAAGGCTG AACCACTAGA AAGACTACAG TCCCAGCACC GACAATTCCA AGCTCGAGTG 600

TCTCGGCAAA CTCTGTTGGC ACTTGAGAAA GAAGAAGAGG AGGAAGTTTT TGAGTCTTCT 660

GTACCACAAC GAAGCACACT AGCTGAACTA AAGAGCAAAG GGAAAAAGAC AGCAAGAGCT 720

CCAATCATCC GTGTAGGAGG TGCTCTCAAG GCTCCAAGCC AGAACAGAGG ACTCCAAAAT 780

CCATTTCCTC AACAGATGCA AAATAATAGT AGAATTACTG TTTTTGATGA AAATGCTGAT 840

GAGGCTTCTA CAGCAGAGTT GTCTAAGCCT ACAGTCCAGC CATGGATAGC ACCCCCCATG 900

CCCAGGGCCA AAGAGAATGA GCTGCAAGCA GGCCCTTGGA ACACAGGCAG GTCCTTGGAA 960

CACAGGCCTC GTGGCAATAC AGCTTCACTG ATAGCTGTAC CCGCTGTGCT TCCCAGTTTC 1020

ACTCCATATG TGGAAGAGAC TGCACGACAG CCAGTTATGA CACCATGTAA AATTGAACCT 1080

AGTATAAACC ACATCCTAAG CACCAGAAAG CCTGGAAAGG AAGAAGGAGA TCCTCTACAA 1140

AGGGTTCAGA GCCATCAGCA AGCGTCTGAG GAGAAGAAAG AGAAGATGAT GTATTGTAAG 1200

GAGAAGATTT ATGCAGGAGT AGGGGAATTC TCCTTTGAAG AAATTCGGGC TGAAGTTTTC 1260

CGGAAGAAAT TAAAAGAGCA AAGGGAAGCC GAGCTATTGA CCAGTGCAGA GAAGAGAGCA 1320

GAAATGCAGA AACAGATTGA AGAGATGGAG AAGAAGCTAA AAGAAATCCA AACTACTCAG 1380

CAAGAAAGAA CAGGTGATCA GCAAGAAGAG ACGATGCCTA CAAAGGAGAC AACTAAACTG 1440

CAAATTGCTT CCGAGTCTCA GAAAATACCA GGAATGACTC TATCCAGTTC TGTTTGTCAA 1500

GTAAACTGTT GTGCCAGAGA AACTTCACTT GCGGAGAACA TTTGGCAGGA ACAACCTCAT 1560

TCTAAAGGTC CCAGTGTACC TTTCTCCATT TTTGATGAGT TTCTTCTTTC AGAAAAGAAG 1620

AATAAAAGTC CTCCTGCAGA TCCCCCACGA GTTTTAGCTC AACGAAGACC CCTTGCAGTT 1680

CTCAAAACCT CAGAAAGCAT CACCTCAAAT GAAGATGTGT CTCCAGATGT TTGTGATGAA 1740

TTTACAGGAA TTGAACCCTT GAGCGAGGAT GCCATTATCA CAGGCTTCAG AAATGTAACA 1800

ATTTGTCCTA ACCCAGAAGA CACTTGTGAC TTTGCCAGAG CAGCTCGTTT TGTATCCACT 1860

CCTTTTCATG AGATAATGTC CTTGAAGGAT CTCCCTTCTG ATCCTGAGAG ACTGTTACCG 1920

GAAGAAGATC TAGATGTAAA GACCTCTGAG GACCAGCAGA CAGCTTGTGG CACTATCTAC 1980

AGTCAGACTC TCAGCATCAA GAAGCTGAGC CCAATTATTG AAGACAGTCG TGAAGCCACA 2040

CACTCCTCTG GCTTCTCTGG TTCTTCTGCC TCGGTTGCAA GCACCTCCTC CATCAAATGT 2100

CTTCAAATTC CTGAGAAACT AGAACTTACT AATGAGACTT CAGAAAACCC TACTCAGTCA 2160

CCATGGTGTT CACAGTATCG CAGACAGCTA CTGAAGTCCC TACCAGAGTT AAGTGCCTCT 2220

GCAGAGTTGT GTATAGAAGA CAGACCAATG CCTAAGTTGG AAATTGAGAA GGAAATTGAA 2280

TTAGGTAATG AGGATTACTG CATTAAACGA GAATACCTAA TATGTGAAGA TTACAAGTTA 2340

TTCTGGGTGG CGCCAAGAAA CTCTGCAGAA TTAACAGTAA TAAAGGTATC TTCTCAACCT 2400

GTCCCATGGG ACTTTTATAT CAACCTCAAG TTAAAGGAAC GTTTAAATGA AGATTTTGAT 2460

CATTTTTGCA GCTGTTATCA ATATCAAGAT GGCTGTATTG TTTGGCACCA ATATATAAAC 2520

TGCTTCACCC TTCAGGATCT TCTCCAACAC AGTGAATATA TTACCCATGA AATAACAGTG 2580

TTGATTATTT ATAACCTTTT GACAATAGTG GAGATGCTAC ACAAAGCAGA AATAGTCCAT 2640

GGTGACTTGA GTCCAAGGTG TCTGATTCTC AGAAACAGAA TCCACGATCC CTATGATTGT 2700

AACAAGAACA ATCAAGCTTT GAAGATAGTG GACTTTTCCT ACAGTGTTGA CCTTAGGGTG 2760

CAGCTGGATG TTTTTACCCT CAGCGGCTTT CGGACTGTAC AGATCCTGGA AGGACAAAAG 2820

ATCCTGGCTA ACTGTTCTTC TCCCTACCAG GTAGACCTGT TTGGTATAGC AGATTTAGCA 2880

CATTTACTAT TGTTCAAGGA ACACCTACAG GTCTTCTGGG ATGGGTCCTT CTGGAAACTT 2940

AGCCAAAATA TTTCTGAGCT AAAAGATGGT GAATTGTGGA ATAAATTCTT TGTGCGGATT 3000

CTGAATGCCA ATGATGAGGC CACAGTGTCT GTTCTTGGGG AGCTTGCAGC AGAAATGAAT 3060

GGGGTTTTTG ACACTACATT CCAAAGTCAC CTGAACAAAG CCTTATGGAA GGTAGGGAAG 3120

TTAACTAGTC CTGGGGCTTT GCTCTTTCAG TGA 3153

Domain Profile

S: 1     leiekeigeGayakvykaeh....dsk........sgelvalKvekpansWefYIclqlk  48

         leiekei+ G++++++k+e+    d+k        s+el+++Kv++++++W+fYI+l+lk

Q: 753   LEIEKEIELGNEDYCIKREYliceDYKlfwvaprnSAELTVIKVSSQPVPWDFYINLKLK  812

         68******************999988899999999*************************

S: 49    eRlkeeellksiieissallfqdasllvleyasygtlldlinkvkevtnkvmdeelvvll  108

         eRl+e+     +++++s++++qd+++++++y++++tl+dl+++++++t     +e++vl+

Q: 813   ERLNED-----FDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYIT-----HEITVLI  862

         ****99.....*************************************.....*******

S: 109   ivqllkiveklHeveIiHgDlkPdnfllgnrimdkadkdkeekglkliDfgrsiDmkllp  168

         i++ll+ive+lH++eI+HgDl+P++++l+nri+d++d++k++++lk++Df++s+D+++++

Q: 863   IYNLLTIVEMLHKAEIVHGDLSPRCLILRNRIHDPYDCNKNNQALKIVDFSYSVDLRVQL  922

         ************************************************************

S: 169   dgtkfkgkaktdafeciemlagkswsYqvDlfglaavihvllfgkyleviqeggswklkq  228

         d+++++g+++++++e++++la++s++YqvDlfg+a+++h+llf+++l+v+++g+ wkl+q

Q: 923   DVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQVFWDGSFWKLSQ  982

         ************************************************************

S: 229   kikrllkkelwekiferlLNisdastlpsleelve  263

         +i++l++ elw+k+f+r+LN++d++t+++l+el++

Q: 983   NISELKDGELWNKFFVRILNANDEATVSVLGELAA  1017

         ********************************986

Domain Sequence
(FASTA)

LEIEKEIELG NEDYCIKREY LICEDYKLFW VAPRNSAELT VIKVSSQPVP WDFYINLKLK 60

ERLNEDFDHF CSCYQYQDGC IVWHQYINCF TLQDLLQHSE YITHEITVLI IYNLLTIVEM 120

LHKAEIVHGD LSPRCLILRN RIHDPYDCNK NNQALKIVDF SYSVDLRVQL DVFTLSGFRT 180

VQILEGQKIL ANCSSPYQVD LFGIADLAHL LLFKEHLQVF WDGSFWKLSQ NISELKDGEL 240

WNKFFVRILN ANDEATVSVL GELAA 265

Keyword

3D-structure; Acetylation; Alternative splicing; Apoptosis; ATP-binding; Cell cycle; Cell division; Centromere; Chromosome; Complete proteome; Cytoplasm; Cytoskeleton; Disease mutation; Kinase; Kinetochore; Mitosis; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase; Tumor suppressor; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Ictidomys tridecemlineatus"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>

Gene Ontology

GO:0005680	; C:anaphase-promoting complex
GO:0000940	; C:condensed chromosome outer kinetochore
GO:0005829	; C:cytosol
GO:0005815	; C:microtubule organizing center
GO:0048471	; C:perinuclear region of cytoplasm
GO:0051233	; C:spindle midzone
GO:0005524	; F:ATP binding
GO:0004672	; F:protein kinase activity
GO:0004674	; F:protein serine/threonine kinase activity
GO:0031145	; P:anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0006915	; P:apoptotic process
GO:0051301	; P:cell division
GO:0008283	; P:cell proliferation
GO:0007094	; P:mitotic cell cycle spindle assembly checkpoint
GO:0007091	; P:mitotic metaphase/anaphase transition
GO:0000236	; P:mitotic prometaphase
GO:0051436	; P:negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
GO:0048015	; P:phosphatidylinositol-mediated signaling
GO:0034501	; P:protein localization to kinetochore
GO:0007051	; P:spindle organization

KEGG

hsa:701

;

InterPros

IPR011009	; Kinase-like_dom.
IPR013212	; Mad3_BUB1_I.
IPR000719	; Prot_kinase_cat_dom.
IPR015661	; Ser/Thr_kinase_Bub1.

Pfam

PF08311	; Mad3_BUB1_I; 1.
PF00069	; Pkinase; 1.

SMARTs

SM00777

; Mad3_BUB1_I; 1.

Prosites

PS51489	; BUB1_N; 1.
PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; FALSE_NEG.
PS00108	; PROTEIN_KINASE_ST; FALSE_NEG.

Prints

Created Date

20-Feb-2013