EKS-HOS-00409

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00409

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/PDGFR	603.8	5.6E-181	585	920	336

Status

Reviewed

Ensembl Protein

ENSP00000390987

UniProt Accession

P10721; B5A956; D5LXN2; D5M931; F5H8F8; Q6IQ28; Q99662; Q9UM99;

Protein Name

Mast/stem cell growth factor receptor Kit

Protein Synonyms/Alias

SCFR; Piebald trait protein; PBT; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog;

Gene Name

KIT

Gene Synonyms/Alias

KIT; SCFR;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000157404	ENSP00000390987	ENST00000412167
ENSG00000157404	ENSP00000288135	ENST00000288135

Organism

Homo sapiens

Functional Description

Tyrosine-protein kinase that acts as cell-surfacereceptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.

Protein Length

972

Protein Sequence
(FASTA)

MRGARGAWDF LCVLLLLLRV QTGSSQPSVS PGEPSPPSIH PGKSDLIVRV GDEIRLLCTD 60

PGFVKWTFEI LDETNENKQN EWITEKAEAT NTGKYTCTNK HGLSNSIYVF VRDPAKLFLV 120

DRSLYGKEDN DTLVRCPLTD PEVTNYSLKG CQGKPLPKDL RFIPDPKAGI MIKSVKRAYH 180

RLCLHCSVDQ EGKSVLSEKF ILKVRPAFKA VPVVSVSKAS YLLREGEEFT VTCTIKDVSS 240

SVYSTWKREN SQTKLQEKYN SWHHGDFNYE RQATLTISSA RVNDSGVFMC YANNTFGSAN 300

VTTTLEVVDK GFINIFPMIN TTVFVNDGEN VDLIVEYEAF PKPEHQQWIY MNRTFTDKWE 360

DYPKSENESN IRYVSELHLT RLKGTEGGTY TFLVSNSDVN AAIAFNVYVN TKPEILTYDR 420

LVNGMLQCVA AGFPEPTIDW YFCPGTEQRC SASVLPVDVQ TLNSSGPPFG KLVVQSSIDS 480

SAFKHNGTVE CKAYNDVGKT SAYFNFAFKE QIHPHTLFTP LLIGFVIVAG MMCIIVMILT 540

YKYLQKPMYE VQWKVVEEIN GNNYVYIDPT QLPYDHKWEF PRNRLSFGKT LGAGAFGKVV 600

EATAYGLIKS DAAMTVAVKM LKPSAHLTER EALMSELKVL SYLGNHMNIV NLLGACTIGG 660

PTLVITEYCC YGDLLNFLRR KRDSFICSKQ EDHAEAALYK NLLHSKESSC SDSTNEYMDM 720

KPGVSYVVPT KADKRRSVRI GSYIERDVTP AIMEDDELAL DLEDLLSFSY QVAKGMAFLA 780

SKNCIHRDLA ARNILLTHGR ITKICDFGLA RDIKNDSNYV VKGNARLPVK WMAPESIFNC 840

VYTFESDVWS YGIFLWELFS LGSSPYPGMP VDSKFYKMIK EGFRMLSPEH APAEMYDIMK 900

TCWDADPLKR PTFKQIVQLI EKQISESTNH IYSNLANCSP NRQKPVVDHS VRINSVGSTA 960

SSSQPLLVHD DV 972

Nucleotide Sequence
(FASTA)

ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCCTACT GCTTCGCGTC 60

CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT 120

CCAGGAAAAT CAGACTTAAT AGTCCGCGTG GGCGACGAGA TTAGGCTGTT ATGCACTGAT 180

CCGGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT 240

GAATGGATCA CGGAAAAGGC AGAAGCCACC AACACCGGCA AATACACGTG CACCAACAAA 300

CACGGCTTAA GCAATTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT 360

GACCGCTCCT TGTATGGGAA AGAAGACAAC GACACGCTGG TCCGCTGTCC TCTCACAGAC 420

CCAGAAGTGA CCAATTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG 480

AGGTTTATTC CTGACCCCAA GGCGGGCATC ATGATCAAAA GTGTGAAACG CGCCTACCAT 540

CGGCTCTGTC TGCATTGTTC TGTGGACCAG GAGGGCAAGT CAGTGCTGTC GGAAAAATTC 600

ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTGTC CAAAGCAAGC 660

TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACGTGCA CAATAAAAGA TGTGTCTAGT 720

TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGACTA AACTACAGGA GAAATATAAT 780

AGCTGGCATC ACGGTGACTT CAATTATGAA CGTCAGGCAA CGTTGACTAT CAGTTCAGCG 840

AGAGTTAATG ATTCTGGAGT GTTCATGTGT TATGCCAATA ATACTTTTGG ATCAGCAAAT 900

GTCACAACAA CCTTGGAAGT AGTAGATAAA GGATTCATTA ATATCTTCCC CATGATAAAC 960

ACTACAGTAT TTGTAAACGA TGGAGAAAAT GTAGATTTGA TTGTTGAATA TGAAGCATTC 1020

CCCAAACCTG AACACCAGCA GTGGATCTAT ATGAACAGAA CCTTCACTGA TAAATGGGAA 1080

GATTATCCCA AGTCTGAGAA TGAAAGTAAT ATCAGATACG TAAGTGAACT TCATCTAACG 1140

AGATTAAAAG GCACCGAAGG AGGCACTTAC ACATTCCTAG TGTCCAATTC TGACGTCAAT 1200

GCTGCCATAG CATTTAATGT TTATGTGAAT ACAAAACCAG AAATCCTGAC TTACGACAGG 1260

CTCGTGAATG GCATGCTCCA ATGTGTGGCA GCAGGATTCC CAGAGCCCAC AATAGATTGG 1320

TATTTTTGTC CAGGAACTGA GCAGAGATGC TCTGCTTCTG TACTGCCAGT GGATGTGCAG 1380

ACACTAAACT CATCTGGGCC ACCGTTTGGA AAGCTAGTGG TTCAGAGTTC TATAGATTCT 1440

AGTGCATTCA AGCACAATGG CACGGTTGAA TGTAAGGCTT ACAACGATGT GGGCAAGACT 1500

TCTGCCTATT TTAACTTTGC ATTTAAAGAG CAAATCCATC CCCACACCCT GTTCACTCCT 1560

TTGCTGATTG GTTTCGTAAT CGTAGCTGGC ATGATGTGCA TTATTGTGAT GATTCTGACC 1620

TACAAATATT TACAGAAACC CATGTATGAA GTACAGTGGA AGGTTGTTGA GGAGATAAAT 1680

GGAAACAATT ATGTTTACAT AGACCCAACA CAACTTCCTT ATGATCACAA ATGGGAGTTT 1740

CCCAGAAACA GGCTGAGTTT TGGGAAAACC CTGGGTGCTG GAGCTTTCGG GAAGGTTGTT 1800

GAGGCAACTG CTTATGGCTT AATTAAGTCA GATGCGGCCA TGACTGTCGC TGTAAAGATG 1860

CTCAAGCCGA GTGCCCATTT GACAGAACGG GAAGCCCTCA TGTCTGAACT CAAAGTCCTG 1920

AGTTACCTTG GTAATCACAT GAATATTGTG AATCTACTTG GAGCCTGCAC CATTGGAGGG 1980

CCCACCCTGG TCATTACAGA ATATTGTTGC TATGGTGATC TTTTGAATTT TTTGAGAAGA 2040

AAACGTGATT CATTTATTTG TTCAAAGCAG GAAGATCATG CAGAAGCTGC ACTTTATAAG 2100

AATCTTCTGC ATTCAAAGGA GTCTTCCTGC AGCGATAGTA CTAATGAGTA CATGGACATG 2160

AAACCTGGAG TTTCTTATGT TGTCCCAACC AAGGCCGACA AAAGGAGATC TGTGAGAATA 2220

GGCTCATACA TAGAAAGAGA TGTGACTCCC GCCATCATGG AGGATGACGA GTTGGCCCTA 2280

GACTTAGAAG ACTTGCTGAG CTTTTCTTAC CAGGTGGCAA AGGGCATGGC TTTCCTCGCC 2340

TCCAAGAATT GTATTCACAG AGACTTGGCA GCCAGAAATA TCCTCCTTAC TCATGGTCGG 2400

ATCACAAAGA TTTGTGATTT TGGTCTAGCC AGAGACATCA AGAATGATTC TAATTATGTG 2460

GTTAAAGGAA ACGCTCGACT ACCTGTGAAG TGGATGGCAC CTGAAAGCAT TTTCAACTGT 2520

GTATACACGT TTGAAAGTGA CGTCTGGTCC TATGGGATTT TTCTTTGGGA GCTGTTCTCT 2580

TTAGGAAGCA GCCCCTATCC TGGAATGCCG GTCGATTCTA AGTTCTACAA GATGATCAAG 2640

GAAGGCTTCC GGATGCTCAG CCCTGAACAC GCACCTGCTG AAATGTATGA CATAATGAAG 2700

ACTTGCTGGG ATGCAGATCC CCTAAAAAGA CCAACATTCA AGCAAATTGT TCAGCTAATT 2760

GAGAAGCAGA TTTCAGAGAG CACCAATCAT ATTTACTCCA ACTTAGCAAA CTGCAGCCCC 2820

AACCGACAGA AGCCCGTGGT AGACCATTCT GTGCGGATCA ATTCTGTCGG CAGCACCGCT 2880

TCCTCCTCCC AGCCTCTGCT TGTGCACGAC GATGTCTGA 2919

Domain Profile

S: 1     lelGkvlGsGafGkvveatayGlsksdavlkvavkmlkstarssekealmselkilshlG  60

         l++Gk+lG+GafGkvveatayGl+ksda+++vavkmlk++a+ +e+ealmselk+ls+lG

Q: 585   LSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLG  644

         79**********************************************************

S: 61    ehenivnllGactkgGpiyviteyccyGdllnylrrkresflkkslekkkekeeeldska  120

         +h+nivnllGact gGp++viteyccyGdlln+lrrkr+sf+   ++k+++++e++ +k+

Q: 645   NHMNIVNLLGACTIGGPTLVITEYCCYGDLLNFLRRKRDSFI---CSKQEDHAEAALYKN  701

         ******************************************...99999*********9

S: 121   lladkslrsksslssesseeyvdlkkdvsdvssskadklasekkksalerevkaaleeed  180

         l     l+sk+s++s+s++ey+d+k++vs+v ++kadk++s ++ s++er+v++a++e+d

Q: 702   L-----LHSKESSCSDSTNEYMDMKPGVSYVVPTKADKRRSVRIGSYIERDVTPAIMEDD  756

         9.....9*****************************************************

S: 181   sevleledllsfsyqvakGmeflaskncvhrdlaarnvllteGkvvkicdfGlardimkd  240

         + +l+ledllsfsyqvakGm+flasknc+hrdlaarn+llt+G+++kicdfGlardi++d

Q: 757   ELALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIKND  816

         ************************************************************

S: 241   snyvvkGnarlpvkwmapesifdslytvksdvwsyGillweifslGstpypgllvdskfy  300

         snyvvkGnarlpvkwmapesif+++yt++sdvwsyGi+lwe+fslGs+pypg++vdskfy

Q: 817   SNYVVKGNARLPVKWMAPESIFNCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFY  876

         ************************************************************

S: 301   klikeGyrmaqpayateeiyeimqkcweaepekrpsfkqlvell  344

         k+ikeG+rm +p++a++e+y+im++cw+a+p krp+fkq+v+l+

Q: 877   KMIKEGFRMLSPEHAPAEMYDIMKTCWDADPLKRPTFKQIVQLI  920

         *****************************************996

Domain Sequence
(FASTA)

LSFGKTLGAG AFGKVVEATA YGLIKSDAAM TVAVKMLKPS AHLTEREALM SELKVLSYLG 60

NHMNIVNLLG ACTIGGPTLV ITEYCCYGDL LNFLRRKRDS FICSKQEDHA EAALYKNLLH 120

SKESSCSDST NEYMDMKPGV SYVVPTKADK RRSVRIGSYI ERDVTPAIME DDELALDLED 180

LLSFSYQVAK GMAFLASKNC IHRDLAARNI LLTHGRITKI CDFGLARDIK NDSNYVVKGN 240

ARLPVKWMAP ESIFNCVYTF ESDVWSYGIF LWELFSLGSS PYPGMPVDSK FYKMIKEGFR 300

MLSPEHAPAE MYDIMKTCWD ADPLKRPTFK QIVQLI 336

Keyword

3D-structure; Alternative splicing; ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; Disease mutation; Disulfide bond; Glycoprotein; Immunoglobulin domain; Kinase; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Polymorphism; Proto-oncogene; Receptor; Reference proteome; Repeat; Signal; Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0009897	; C:external side of plasma membrane
GO:0005615	; C:extracellular space
GO:0016021	; C:integral to membrane
GO:0005634	; C:nucleus
GO:0005886	; C:plasma membrane
GO:0005524	; F:ATP binding
GO:0019955	; F:cytokine binding
GO:0046872	; F:metal ion binding
GO:0004716	; F:receptor signaling protein tyrosine kinase activity
GO:0005020	; F:stem cell factor receptor activity
GO:0004714	; F:transmembrane receptor protein tyrosine kinase activity
GO:0031532	; P:actin cytoskeleton reorganization
GO:0000187	; P:activation of MAPK activity
GO:0097067	; P:cellular response to thyroid hormone stimulus
GO:0002371	; P:dendritic cell cytokine production
GO:0050910	; P:detection of mechanical stimulus involved in sensory perception of sound
GO:0048565	; P:digestive tract development
GO:0035162	; P:embryonic hemopoiesis
GO:0030218	; P:erythrocyte differentiation
GO:0038162	; P:erythropoietin-mediated signaling pathway
GO:0038093	; P:Fc receptor signaling pathway
GO:0035234	; P:germ cell programmed cell death
GO:0006687	; P:glycosphingolipid metabolic process
GO:0002327	; P:immature B cell differentiation
GO:0006954	; P:inflammatory response
GO:0007243	; P:intracellular protein kinase cascade
GO:0038109	; P:Kit signaling pathway
GO:0030032	; P:lamellipodium assembly
GO:0002320	; P:lymphoid progenitor cell differentiation
GO:0008584	; P:male gonad development
GO:0002551	; P:mast cell chemotaxis
GO:0032762	; P:mast cell cytokine production
GO:0043303	; P:mast cell degranulation
GO:0060374	; P:mast cell differentiation
GO:0070662	; P:mast cell proliferation
GO:0035855	; P:megakaryocyte development
GO:0097326	; P:melanocyte adhesion
GO:0030318	; P:melanocyte differentiation
GO:0097324	; P:melanocyte migration
GO:0002318	; P:myeloid progenitor cell differentiation
GO:0043069	; P:negative regulation of programmed cell death
GO:0001541	; P:ovarian follicle development
GO:0018108	; P:peptidyl-tyrosine phosphorylation
GO:0008284	; P:positive regulation of cell proliferation
GO:0010628	; P:positive regulation of gene expression
GO:0043410	; P:positive regulation of MAPK cascade
GO:0043552	; P:positive regulation of phosphatidylinositol 3-kinase activity
GO:0014068	; P:positive regulation of phosphatidylinositol 3-kinase cascade
GO:0010863	; P:positive regulation of phospholipase C activity
GO:0051091	; P:positive regulation of sequence-specific DNA binding transcription factor activity
GO:0042511	; P:positive regulation of tyrosine phosphorylation of Stat1 protein
GO:0042517	; P:positive regulation of tyrosine phosphorylation of Stat3 protein
GO:0042523	; P:positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0046777	; P:protein autophosphorylation
GO:0042127	; P:regulation of cell proliferation
GO:0008360	; P:regulation of cell shape
GO:0048070	; P:regulation of developmental pigmentation
GO:0009314	; P:response to radiation
GO:0007286	; P:spermatid development
GO:0007283	; P:spermatogenesis
GO:0019827	; P:stem cell maintenance
GO:0030217	; P:T cell differentiation

KEGG

hsa:3815

;

InterPros

IPR007110	; Ig-like.
IPR013783	; Ig-like_fold.
IPR003599	; Ig_sub.
IPR003598	; Ig_sub2.
IPR013151	; Immunoglobulin.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.
IPR016243	; Tyr_kinase_CSF1/PDGF_rcpt.
IPR001824	; Tyr_kinase_rcpt_3_CS.

Pfam

PF00047	; ig; 1.
PF07714	; Pkinase_Tyr; 1.

SMARTs

SM00409	; IG; 1.
SM00408	; IGc2; 1.
SM00219	; TyrKc; 1.

Prosites

PS50835	; IG_LIKE; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS00240	; RECEPTOR_TYR_KIN_III; 1.

Prints

Created Date

20-Feb-2013