EKS-HOS-00259

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00259

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CAMK/CAMKL	327.5	2.5E-97	53	303	251

Status

Reviewed

Ensembl Protein

ENSP00000356125

UniProt Accession

Q9H093;

Protein Name

NUAK family SNF1-like kinase 2

Protein Synonyms/Alias

Omphalocele kinase 2; SNF1/AMP kinase-related kinase; SNARK;

Gene Name

NUAK2

Gene Synonyms/Alias

NUAK2; OMPHK2, SNARK;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000163545	ENSP00000356125	ENST00000367157

Organism

Homo sapiens

Functional Description

Stress-activated kinase involved in tolerance to glucosestarvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95- activated tumor cells. Able to phosphorylate 'Ser-464' of LATS1.

Protein Length

628

Protein Sequence
(FASTA)

MESLVFARRS GPTPSAAELA RPLAEGLIKS PKPLMKKQAV KRHHHKHNLR HRYEFLETLG 60

KGTYGKVKKA RESSGRLVAI KSIRKDKIKD EQDLMHIRRE IEIMSSLNHP HIIAIHEVFE 120

NSSKIVIVME YASRGDLYDY ISERQQLSER EARHFFRQIV SAVHYCHQNR VVHRDLKLEN 180

ILLDANGNIK IADFGLSNLY HQGKFLQTFC GSPLYASPEI VNGKPYTGPE VDSWSLGVLL 240

YILVHGTMPF DGHDHKILVK QISNGAYREP PKPSDACGLI RWLLMVNPTR RATLEDVASH 300

WWVNWGYATR VGEQEAPHEG GHPGSDSARA SMADWLRRSS RPLLENGAKV CSFFKQHAPG 360

GGSTTPGLER QHSLKKSRKE NDMAQSLHSD TADDTAHRPG KSNLKLPKGI LKKKVSASAE 420

GVQEDPPELS PIPASPGQAA PLLPKKGILK KPRQRESGYY SSPEPSESGE LLDAGDVFVS 480

GDPKEQKPPQ ASGLLLHRKG ILKLNGKFSQ TALELAAPTT FGSLDELAPP RPLARASRPS 540

GAVSEDSILS SESFDQLDLP ERLPEPPLRG CVSVDNLTGL EEPPSEGPGS CLRRWRQDPL 600

GDSCFSLTDC QEVTATYRQA LRVCSKLT 628

Nucleotide Sequence
(FASTA)

ATGGAGTCGC TGGTTTTCGC GCGGCGCTCC GGCCCCACTC CCTCGGCCGC AGAGCTAGCC 60

CGGCCGCTGG CGGAAGGGCT GATCAAGTCG CCCAAGCCCC TAATGAAGAA GCAGGCGGTG 120

AAGCGGCACC ACCACAAGCA CAACCTGCGG CACCGCTACG AGTTCCTGGA GACCCTGGGC 180

AAAGGCACCT ACGGGAAGGT GAAGAAGGCG CGGGAGAGCT CGGGGCGCCT GGTGGCCATC 240

AAGTCAATCC GGAAGGACAA AATCAAAGAT GAGCAAGATC TGATGCACAT ACGGAGGGAG 300

ATTGAGATCA TGTCATCACT CAACCACCCT CACATCATTG CCATCCATGA AGTGTTTGAG 360

AACAGCAGCA AGATCGTGAT CGTCATGGAG TATGCCAGCC GGGGCGACCT TTATGACTAC 420

ATCAGCGAGC GGCAGCAGCT CAGTGAGCGC GAAGCTAGGC ATTTCTTCCG GCAGATCGTC 480

TCTGCCGTGC ACTATTGCCA TCAGAACAGA GTTGTCCACC GAGATCTCAA GCTGGAGAAC 540

ATCCTCTTGG ATGCCAATGG GAATATCAAG ATTGCTGACT TCGGTCTCTC CAACCTCTAC 600

CATCAAGGCA AGTTCCTGCA GACATTCTGT GGGAGCCCCC TCTATGCCTC GCCAGAGATT 660

GTCAATGGGA AGCCCTACAC AGGCCCAGAG GTGGACAGCT GGTCCCTGGG TGTTCTCCTC 720

TACATCCTGG TGCATGGCAC CATGCCCTTT GATGGGCATG ACCATAAGAT CCTAGTGAAA 780

CAGATCAGCA ACGGGGCCTA CCGGGAGCCA CCTAAACCCT CTGATGCCTG TGGCCTGATC 840

CGGTGGCTGT TGATGGTGAA CCCCACCCGC CGGGCCACCC TGGAGGATGT GGCCAGTCAC 900

TGGTGGGTCA ACTGGGGCTA CGCCACCCGA GTGGGAGAGC AGGAGGCTCC GCATGAGGGT 960

GGGCACCCTG GCAGTGACTC TGCCCGCGCC TCCATGGCTG ACTGGCTCCG GCGTTCCTCC 1020

CGCCCCCTCC TGGAGAATGG GGCCAAGGTG TGCAGCTTCT TCAAGCAGCA TGCACCTGGT 1080

GGGGGAAGCA CCACCCCTGG CCTGGAGCGC CAGCATTCGC TCAAGAAGTC CCGCAAGGAG 1140

AATGACATGG CCCAGTCTCT CCACAGTGAC ACGGCTGATG ACACTGCCCA TCGCCCTGGC 1200

AAGAGCAACC TCAAGCTGCC AAAGGGCATT CTCAAGAAGA AGGTGTCAGC CTCTGCAGAA 1260

GGGGTACAGG AGGACCCTCC GGAGCTCAGC CCAATCCCTG CGAGCCCAGG GCAGGCTGCC 1320

CCCCTGCTCC CCAAGAAGGG CATTCTCAAG AAGCCCCGAC AGCGCGAGTC TGGCTACTAC 1380

TCCTCTCCCG AGCCCAGTGA ATCTGGGGAG CTCTTGGACG CAGGCGACGT GTTTGTGAGT 1440

GGGGATCCCA AGGAGCAGAA GCCTCCGCAA GCTTCAGGGC TGCTCCTCCA TCGCAAAGGC 1500

ATCCTCAAAC TCAATGGCAA GTTCTCCCAG ACAGCCTTGG AGCTCGCGGC CCCCACCACC 1560

TTCGGCTCCC TGGATGAACT CGCCCCACCT CGCCCCCTGG CCCGGGCCAG CCGACCCTCA 1620

GGGGCTGTGA GCGAGGACAG CATCCTGTCC TCTGAGTCCT TTGACCAGCT GGACTTGCCT 1680

GAACGGCTCC CAGAGCCCCC ACTGCGGGGC TGTGTGTCTG TGGACAACCT CACGGGGCTT 1740

GAGGAGCCCC CCTCAGAGGG CCCTGGAAGC TGCCTGAGGC GCTGGCGGCA GGATCCTTTG 1800

GGGGACAGCT GCTTTTCCCT GACAGACTGC CAGGAGGTGA CAGCGACCTA CCGACAGGCA 1860

CTGAGGGTCT GCTCAAAGCT CACCTGA 1887

Domain Profile

S: 1     ykllktlGegsfgkVklakhketkekvavKiikkkkl.seeslekikrEieilkklkhpn  59

         y++l+tlG+g++gkVk+a+++  ++ va+K i+k+k+ +e+ l +i+rEiei+++l+hp+

Q: 53    YEFLETLGKGTYGKVKKARES-SGRLVAIKSIRKDKIkDEQDLMHIRREIEIMSSLNHPH  111

         99***************9986.799**********996778899****************

S: 60    iikllevietkkklylvleyasggeLfdyieekgrlkekearklfkqlvsavkYlhskgi  119

         ii ++ev+e+++k+++v+eyas+g+L+dyi+e+++l+e+ear++f+q+vsav+Y+h++++

Q: 112   IIAIHEVFENSSKIVIVMEYASRGDLYDYISERQQLSEREARHFFRQIVSAVHYCHQNRV  171

         ************************************************************

S: 120   vHrDlKpeNllldkkenikiaDFGlanlfkegklletfcGsppYaaPellkgkkYegpkv  179

         vHrDlK+eN+lld+++nikiaDFGl+nl+++gk l+tfcGsp Ya+Pe+++gk+Y+gp+v

Q: 172   VHRDLKLENILLDANGNIKIADFGLSNLYHQGKFLQTFCGSPLYASPEIVNGKPYTGPEV  231

         ************************************************************

S: 180   DvWslGvvLyalvtgklPfdeenlkellekilkgkleipkklskelesLlrklLvvdpek  239

         D WslGv+Ly+lv g++Pfd+++ k l+++i++g ++ p k s ++  L+r lL+v+p++

Q: 232   DSWSLGVLLYILVHGTMPFDGHDHKILVKQISNGAYREPPKPS-DACGLIRWLLMVNPTR  290

         ****************************************966.5668************

S: 240   Ratieeilkhewl  252

         Rat+e++ +h w+

Q: 291   RATLEDVASHWWV  303

         ************6

Domain Sequence
(FASTA)

YEFLETLGKG TYGKVKKARE SSGRLVAIKS IRKDKIKDEQ DLMHIRREIE IMSSLNHPHI 60

IAIHEVFENS SKIVIVMEYA SRGDLYDYIS ERQQLSEREA RHFFRQIVSA VHYCHQNRVV 120

HRDLKLENIL LDANGNIKIA DFGLSNLYHQ GKFLQTFCGS PLYASPEIVN GKPYTGPEVD 180

SWSLGVLLYI LVHGTMPFDG HDHKILVKQI SNGAYREPPK PSDACGLIRW LLMVNPTRRA 240

TLEDVASHWW V 251

Keyword

Apoptosis; ATP-binding; Complete proteome; Direct protein sequencing; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Dasypus novemcinctus"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005524	; F:ATP binding
GO:0000287	; F:magnesium ion binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0030036	; P:actin cytoskeleton organization
GO:0006915	; P:apoptotic process
GO:0042149	; P:cellular response to glucose starvation
GO:0043066	; P:negative regulation of apoptotic process

KEGG

hsa:81788

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013