EKS-HOS-00066

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00066

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CMGC/CDK	372.3	7.3E-111	726	1019	294

Status

Reviewed

Ensembl Protein

ENSP00000464641

UniProt Accession

Q9NYV4; A7E2B2; B4DYX4; B9EIQ6; O94978;

Protein Name

Cyclin-dependent kinase 12

Protein Synonyms/Alias

Cdc2-related kinase, arginine/serine-rich; CrkRS; Cell division cycle 2-related protein kinase 7; CDC2-related protein kinase 7; Cell division protein kinase 12; hCDK12;

Gene Name

CDK12

Gene Synonyms/Alias

CDK12; CRK7, CRKRS, KIAA0904;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000167258	ENSP00000453329	ENST00000559663
ENSG00000167258	ENSP00000407720	ENST00000430627
ENSG00000167258	ENSP00000464641	ENST00000584632
ENSG00000167258	ENSP00000398880	ENST00000447079

Organism

Homo sapiens

Functional Description

Cyclin-dependent kinase which displays CTD kinaseactivity and is required for RNA splicing. Has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogn inhibitors.

Protein Length

1201

Protein Sequence
(FASTA)

MPNSERHGGK KDGSGGASGT LQPSSGGGSS NSRERHRLVS KHKRHKSKHS KDMGLVTPEA 60

ASLGTVIKPL VEYDDISSDS DTFSDDMAFK LDRRENDERR GSDRSDRLHK HRHHQHRRSR 120

DLLKAKQTEK EKSQEVSSKS GSMKDRISGS SKRSNEETDD YGKAQVAKSS SKESRSSKLH 180

KEKTRKEREL KSGHKDRSKS HRKRETPKSY KTVDSPKRRS RSPHRKWSDS SKQDDSPSGA 240

SYGQDYDLSP SRSHTSSNYD SYKKSPGSTS RRQSVSPPYK EPSAYQSSTR SPSPYSRRQR 300

SVSPYSRRRS SSYERSGSYS GRSPSPYGRR RSSSPFLSKR SLSRSPLPRK SMKSRSRSPA 360

YSRHSSSHSK KKRSSSRSRH SSISPVRLPL NSSLGAELSR KKKERAAAAA AAKMDGKESK 420

GSPVFLPRKE NSSVEAKDSG LESKKLPRSV KLEKSAPDTE LVNVTHLNTE VKNSSDTGKV 480

KLDENSEKHL VKDLKAQGTR DSKPIALKEE IVTPKETETS EKETPPPLPT IASPPPPLPT 540

TTPPPQTPPL PPLPPIPALP QQPPLPPSQP AFSQVPASST STLPPSTHSK TSAVSSQANS 600

QPPVQVSVKT QVSVTAAIPH LKTSTLPPLP LPPLLPGDDD MDSPKETLPS KPVKKEKEQR 660

TRHLLTDLPL PPELPGGDLS PPDSPEPKAI TPPQQPYKKR PKICCPRYGE RRQTESDWGK 720

RCVDKFDIIG IIGEGTYGQV YKAKDKDTGE LVALKKVRLD NEKEGFPITA IREIKILRQL 780

IHRSVVNMKE IVTDKQDALD FKKDKGAFYL VFEYMDHDLM GLLESGLVHF SEDHIKSFMK 840

QLMEGLEYCH KKNFLHRDIK CSNILLNNSG QIKLADFGLA RLYNSEESRP YTNKVITLWY 900

RPPELLLGEE RYTPAIDVWS CGCILGELFT KKPIFQANLE LAQLELISRL CGSPCPAVWP 960

DVIKLPYFNT MKPKKQYRRR LREEFSFIPS AALDLLDHML TLDPSKRCTA EQTLQSDFLK 1020

DVELSKMAPP DLPHWQDCHE LWSKKRRRQR QSGVVVEEPP PSKTSRKETT SGTSTEPVKN 1080

SSPAPPQPAP GKVESGAGDA IGLADITQQL NQSELAVLLN LLQSQTDLSI PQMAQLLNIH 1140

SNPEMQQQLE ALNQSISALT EATSQQQDSE TMAPEESLKE APSAPVILPS AEQTTLEASS 1200

T 1201

Nucleotide Sequence
(FASTA)

ATGCCCAATT CAGAGAGACA TGGGGGCAAG AAGGACGGGA GTGGAGGAGC TTCTGGAACT 60

TTGCAGCCGT CATCGGGAGG CGGCAGCTCT AACAGCAGAG AGCGTCACCG CTTGGTATCG 120

AAGCACAAGC GGCATAAGTC CAAACACTCC AAAGACATGG GGTTGGTGAC CCCCGAAGCA 180

GCATCCCTGG GCACAGTTAT CAAACCTTTG GTGGAGTATG ATGATATCAG CTCTGATTCC 240

GACACCTTCT CCGATGACAT GGCCTTCAAA CTAGACCGAA GGGAGAACGA CGAACGTCGT 300

GGATCAGATC GGAGCGACCG CCTGCACAAA CATCGTCACC ACCAGCACAG GCGTTCCCGG 360

GACTTACTAA AAGCTAAACA GACCGAAAAA GAAAAAAGCC AAGAAGTCTC CAGCAAGTCG 420

GGATCGATGA AGGACCGGAT ATCGGGAAGT TCAAAGCGTT CGAATGAGGA GACTGATGAC 480

TATGGGAAGG CGCAGGTAGC CAAAAGCAGC AGCAAGGAAT CCAGGTCATC CAAGCTCCAC 540

AAGGAGAAGA CCAGGAAAGA ACGGGAGCTG AAGTCTGGGC ACAAAGACCG GAGTAAAAGT 600

CATCGAAAAA GGGAAACACC CAAAAGTTAC AAAACAGTGG ACAGCCCAAA ACGGAGATCC 660

AGGAGCCCCC ACAGGAAGTG GTCTGACAGC TCCAAACAAG ATGATAGCCC CTCGGGAGCT 720

TCTTATGGCC AAGATTATGA CCTTAGTCCC TCACGATCTC ATACCTCGAG CAATTATGAC 780

TCCTACAAGA AAAGTCCTGG AAGTACCTCG AGAAGGCAGT CGGTCAGTCC CCCTTACAAG 840

GAGCCTTCGG CCTACCAGTC CAGCACCCGG TCACCGAGCC CCTACAGTAG GCGACAGAGA 900

TCTGTCAGTC CCTATAGCAG GAGACGGTCG TCCAGCTACG AAAGAAGTGG CTCTTACAGC 960

GGGCGATCGC CCAGTCCCTA TGGTCGAAGG CGGTCCAGCA GCCCTTTCCT GAGCAAGCGG 1020

TCTCTGAGTC GGAGTCCACT CCCCAGGAAA TCCATGAAGT CCAGAAGTAG AAGTCCTGCA 1080

TATTCAAGAC ATTCATCTTC TCATAGTAAA AAGAAGAGAT CCAGTTCACG CAGTCGTCAT 1140

TCCAGTATCT CACCTGTCAG GCTTCCACTT AATTCCAGTC TGGGAGCTGA ACTCAGTAGG 1200

AAAAAGAAGG AAAGAGCAGC TGCTGCTGCT GCAGCAAAGA TGGATGGAAA GGAGTCCAAG 1260

GGTTCACCTG TATTTTTGCC TAGAAAAGAG AACAGTTCAG TAGAGGCTAA GGATTCAGGT 1320

TTGGAGTCTA AAAAGTTACC CAGAAGTGTA AAATTGGAAA AATCTGCCCC AGATACTGAA 1380

CTGGTGAATG TAACACATCT AAACACAGAG GTAAAAAATT CTTCAGATAC AGGGAAAGTA 1440

AAGTTGGATG AGAACTCCGA GAAGCATCTT GTTAAAGATT TGAAAGCACA GGGAACAAGA 1500

GACTCTAAAC CCATAGCACT GAAAGAGGAG ATTGTTACTC CAAAGGAGAC AGAAACATCA 1560

GAAAAGGAGA CCCCTCCACC TCTTCCCACA ATTGCTTCTC CCCCACCCCC TCTACCAACT 1620

ACTACCCCTC CACCTCAGAC ACCCCCTTTG CCACCTTTGC CTCCAATACC AGCTCTTCCA 1680

CAGCAACCAC CTCTGCCTCC TTCTCAGCCA GCATTTAGTC AGGTTCCTGC TTCCAGTACT 1740

TCAACTTTGC CCCCTTCTAC TCACTCAAAG ACATCTGCTG TGTCCTCTCA GGCAAATTCT 1800

CAGCCCCCTG TACAGGTTTC TGTGAAGACT CAAGTATCTG TAACAGCTGC TATTCCACAC 1860

CTGAAAACTT CAACGTTGCC TCCTTTGCCC CTCCCACCCT TATTACCTGG AGATGATGAC 1920

ATGGATAGTC CAAAAGAAAC TCTTCCTTCA AAACCTGTGA AGAAAGAGAA GGAACAGAGG 1980

ACACGTCACT TACTCACAGA CCTTCCTCTC CCTCCAGAGC TCCCTGGTGG AGATCTGTCT 2040

CCCCCAGACT CTCCAGAACC AAAGGCAATC ACACCACCTC AGCAACCATA TAAAAAGAGA 2100

CCAAAAATTT GTTGTCCTCG TTATGGAGAA AGAAGACAAA CAGAAAGCGA CTGGGGGAAA 2160

CGCTGTGTGG ACAAGTTTGA CATTATTGGG ATTATTGGAG AAGGAACCTA TGGCCAAGTA 2220

TATAAAGCCA AGGACAAAGA CACAGGAGAA CTAGTGGCTC TGAAGAAGGT GAGACTAGAC 2280

AATGAGAAAG AGGGCTTCCC AATCACAGCC ATTCGTGAAA TCAAAATCCT TCGTCAGTTA 2340

ATCCACCGAA GTGTTGTTAA CATGAAGGAA ATTGTCACAG ATAAACAAGA TGCACTGGAT 2400

TTCAAGAAGG ACAAAGGTGC CTTTTACCTT GTATTTGAGT ATATGGACCA TGACTTAATG 2460

GGACTGCTAG AATCTGGTTT GGTGCACTTT TCTGAGGACC ATATCAAGTC GTTCATGAAA 2520

CAGCTAATGG AAGGATTGGA ATACTGTCAC AAAAAGAATT TCCTGCATCG GGATATTAAG 2580

TGTTCTAACA TTTTGCTGAA TAACAGTGGG CAAATCAAAC TAGCAGATTT TGGACTTGCT 2640

CGGCTCTATA ACTCTGAAGA GAGTCGCCCT TACACAAACA AAGTCATTAC TTTGTGGTAC 2700

CGACCTCCAG AACTACTGCT AGGAGAGGAA CGTTACACAC CAGCCATAGA TGTTTGGAGC 2760

TGTGGATGTA TTCTTGGGGA ACTATTCACA AAGAAGCCTA TTTTTCAAGC CAATCTGGAA 2820

CTGGCTCAGC TAGAACTGAT CAGCCGACTT TGTGGTAGCC CTTGTCCAGC TGTGTGGCCT 2880

GATGTTATCA AACTGCCCTA CTTCAACACC ATGAAACCGA AGAAGCAATA TCGAAGGCGT 2940

CTACGAGAAG AATTCTCTTT CATTCCTTCT GCAGCACTTG ATTTATTGGA CCACATGCTG 3000

ACACTAGATC CTAGTAAGCG GTGCACAGCT GAACAGACCC TACAGAGCGA CTTCCTTAAA 3060

GATGTCGAAC TCAGCAAAAT GGCTCCTCCA GACCTCCCCC ACTGGCAGGA TTGCCATGAG 3120

TTGTGGAGTA AGAAACGGCG ACGTCAGCGA CAAAGTGGTG TTGTAGTCGA AGAGCCACCT 3180

CCATCCAAAA CTTCTCGAAA AGAAACTACC TCAGGGACAA GTACTGAGCC TGTGAAGAAC 3240

AGCAGCCCAG CACCACCTCA GCCTGCTCCT GGCAAGGTGG AGTCTGGGGC TGGGGATGCA 3300

ATAGGCCTTG CTGACATCAC ACAACAGCTG AATCAAAGTG AATTGGCAGT GTTATTAAAC 3360

CTGCTGCAGA GCCAAACCGA CCTGAGCATC CCTCAAATGG CACAGCTGCT TAACATCCAC 3420

TCCAACCCAG AGATGCAGCA GCAGCTGGAA GCCCTGAACC AATCCATCAG TGCCCTGACG 3480

GAAGCTACTT CCCAGCAGCA GGACTCAGAG ACCATGGCCC CAGAGGAGTC TTTGAAGGAA 3540

GCACCCTCTG CCCCAGTGAT CCTGCCTTCA GCAGAACAGA CGACCCTTGA AGCTTCAAGC 3600

ACAC 3604

Domain Profile

S: 1     yeklekigeGtygkvykakdketgevvAlKkirlekekegvpitalrEisllkelkhkni  60

         ++ +  igeGtyg+vykakdk+tge+vAlKk+rl++ekeg+pita+rEi++l++l h+ +

Q: 726   FDIIGIIGEGTYGQVYKAKDKDTGELVALKKVRLDNEKEGFPITAIREIKILRQLIHRSV  785

         667889******************************************************

S: 61    vkLlevva.........kdkkklyLvfefleqdLkkllkkkkkkklsleevkslllqlle  111

         v+++e+v+         kdk  +yLvfe++++dL+ ll++    ++s++++ks+++ql+e

Q: 786   VNMKEIVTdkqdaldfkKDKGAFYLVFEYMDHDLMGLLESGLV-HFSEDHIKSFMKQLME  844

         ****************8899********************999.****************

S: 112   glaylHsnkilHRDlKpqNlLiskegklklaDfGlarafssplk.tytkevvTlwYraPe  170

         gl+y+H++++lHRD+K++N+L++++g++klaDfGlar ++s+++  yt++v+TlwYr+Pe

Q: 845   GLEYCHKKNFLHRDIKCSNILLNNSGQIKLADFGLARLYNSEESrPYTNKVITLWYRPPE  904

         ***************************************7766527**************

S: 171   lLlgakeYstavDiWsvgcilaelltrkplfqgkseidqlerifkllgtpsekvwpevsk  230

         lLlg+++Y+ a+D+Ws+gcil el+t+kp+fq++ e  qle i++l+g+p  +vwp+v k

Q: 905   LLLGEERYTPAIDVWSCGCILGELFTKKPIFQANLELAQLELISRLCGSPCPAVWPDVIK  964

         ************************************************************

S: 231   lpeykksfpkkkkk.klkekvkklkekaldllekllaldpkkRisakealqhkyf  284

         lp +++  pkk+++ +l+e+++ + ++aldll+++l+ldp+kR++a+++lq++++

Q: 965   LPYFNTMKPKKQYRrRLREEFSFIPSAALDLLDHMLTLDPSKRCTAEQTLQSDFL  1019

         *****7777777777******9999**************************9985

Domain Sequence
(FASTA)

FDIIGIIGEG TYGQVYKAKD KDTGELVALK KVRLDNEKEG FPITAIREIK ILRQLIHRSV 60

VNMKEIVTDK QDALDFKKDK GAFYLVFEYM DHDLMGLLES GLVHFSEDHI KSFMKQLMEG 120

LEYCHKKNFL HRDIKCSNIL LNNSGQIKLA DFGLARLYNS EESRPYTNKV ITLWYRPPEL 180

LLGEERYTPA IDVWSCGCIL GELFTKKPIF QANLELAQLE LISRLCGSPC PAVWPDVIKL 240

PYFNTMKPKK QYRRRLREEF SFIPSAALDL LDHMLTLDPS KRCTAEQTLQ SDFL 294

Keyword

Acetylation; Alternative splicing; ATP-binding; Chromosomal rearrangement; Complete proteome; Kinase; mRNA processing; mRNA splicing; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gorilla gorilla"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0019908	; C:nuclear cyclin-dependent protein kinase holoenzyme complex
GO:0016607	; C:nuclear speck
GO:0005730	; C:nucleolus
GO:0005524	; F:ATP binding
GO:0004693	; F:cyclin-dependent protein kinase activity
GO:0008353	; F:RNA polymerase II carboxy-terminal domain kinase activity
GO:0007049	; P:cell cycle
GO:0006397	; P:mRNA processing
GO:0070816	; P:phosphorylation of RNA polymerase II C-terminal domain
GO:0046777	; P:protein autophosphorylation
GO:0043405	; P:regulation of MAP kinase activity
GO:0008380	; P:RNA splicing

KEGG

hsa:51755

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013