| Tag | Content |
|---|
| EKPD ID | EKS-HOS-00422 |
| Classification | | Group/Family | Score | E-Value | Start | End | Domain Length |
|---|
| CAMK/DAPK | 481.9 | 2.1E-144 | 13 | 275 | 263 |
|
| Status | Reviewed |
| Ensembl Protein | ENSP00000301264 |
| UniProt Accession | O43293; A0AVN4; B3KQE2; Q05JY4; |
| Protein Name | Death-associated protein kinase 3 |
| Protein Synonyms/Alias | DAP kinase 3; DAP-like kinase; Dlk; MYPT1 kinase; Zipper-interacting protein kinase; ZIP-kinase; |
| Gene Name | DAPK3 |
| Gene Synonyms/Alias | DAPK3; ZIPK; |
| Ensembl Information | |
| Organism | Homo sapiens |
| Functional Description | Serine/threonine kinase which is involved in theregulation of apoptosis, autophagy, transcription, actin cytoskeleton reorganization, cell motility, smooth muscle contraction, and mitosis, particularly cytokinesis. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase- dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Regulates myosin phosphorylation in both smooth muscle and non- muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A, and the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Phosphorylates STAT3 and enhances its transcriptional activity. Positively regulates the canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Can disrupt the NLK-TCF7L2 complex thereby influencing the phosphorylation of TCF7L2 by NLK. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B. |
| Protein Length | 454 |
Protein Sequence
(FASTA) | | MSTFRQEDVE DHYEMGEELG SGQFAIVRKC RQKGTGKEYA AKFIKKRRLS SSRRGVSREE 60 | | IEREVNILRE IRHPNIITLH DIFENKTDVV LILELVSGGE LFDFLAEKES LTEDEATQFL 120 | | KQILDGVHYL HSKRIAHFDL KPENIMLLDK NVPNPRIKLI DFGIAHKIEA GNEFKNIFGT 180 | | PEFVAPEIVN YEPLGLEADM WSIGVITYIL LSGASPFLGE TKQETLTNIS AVNYDFDEEY 240 | | FSNTSELAKD FIRRLLVKDP KRRMTIAQSL EHSWIKAIRR RNVRGEDSGR KPERRRLKTT 300 | | RLKEYTIKSH SSLPPNNSYA DFERFSKVLE EAAAAEEGLR ELQRSRRLCH EDVEALAAIY 360 | | EEKEAWYREE SDSLGQDLRR LRQELLKTEA LKRQAQEEAK GALLGTSGLK RRFSRLENRY 420 | | EALAKQVASE MRFVQDLVRA LEQEKLQGVE CGLR 454 |
|
Nucleotide Sequence
(FASTA) | | ATGTCCACGT TCAGGCAGGA GGACGTGGAG GACCATTATG AGATGGGGGA GGAGCTGGGC 60 | | AGCGGCCAGT TTGCGATCGT GCGGAAGTGC CGGCAGAAGG GCACGGGCAA GGAGTACGCA 120 | | GCCAAGTTCA TCAAGAAGCG CCGCCTGTCA TCCAGCCGGC GTGGGGTGAG CCGGGAGGAG 180 | | ATCGAGCGGG AGGTGAACAT CCTGCGGGAG ATCCGGCACC CCAACATCAT CACCCTGCAC 240 | | GACATCTTCG AGAACAAGAC GGACGTGGTC CTCATCCTGG AGCTGGTCTC TGGCGGGGAG 300 | | CTCTTTGACT TCCTGGCGGA GAAGGAGTCG CTGACGGAGG ACGAGGCCAC CCAGTTCCTC 360 | | AAGCAGATCC TGGACGGCGT TCACTACCTG CACTCTAAGC GCATCGCACA CTTTGACCTG 420 | | AAGCCGGAAA ACATCATGCT GCTGGACAAG AACGTGCCCA ACCCACGAAT CAAGCTCATC 480 | | GACTTCGGCA TCGCGCACAA GATCGAGGCG GGGAACGAGT TCAAGAACAT CTTCGGCACC 540 | | CCGGAGTTTG TGGCCCCAGA GATTGTGAAC TATGAGCCGC TGGGCCTGGA GGCGGACATG 600 | | TGGAGCATCG GTGTCATCAC CTATATCCTC CTGAGCGGTG CATCCCCGTT CCTGGGCGAG 660 | | ACCAAGCAGG AGACGCTCAC CAACATCTCA GCCGTGAACT ACGACTTCGA CGAGGAGTAC 720 | | TTCAGCAACA CCAGCGAGCT GGCCAAGGAC TTCATTCGCC GGCTGCTCGT CAAAGATCCC 780 | | AAGCGGAGAA TGACCATTGC CCAGAGCCTG GAACATTCCT GGATTAAGGC GATCCGGCGG 840 | | CGGAACGTGC GTGGTGAGGA CAGCGGCCGC AAGCCCGAGC GGCGGCGCCT GAAGACCACG 900 | | CGTCTGAAGG AGTACACCAT CAAGTCGCAC TCCAGCTTGC CGCCCAACAA CAGCTACGCC 960 | | GACTTCGAGC GCTTCTCCAA GGTGCTGGAG GAGGCGGCGG CCGCCGAGGA GGGCCTGCGC 1020 | | GAGCTGCAGC GCAGCCGGCG GCTCTGCCAC GAGGACGTGG AGGCGCTGGC CGCCATCTAC 1080 | | GAGGAGAAGG AGGCCTGGTA CCGCGAGGAG AGCGACAGCC TGGGCCAGGA CCTGCGGAGG 1140 | | CTACGGCAGG AGCTGCTCAA GACCGAGGCG CTCAAGCGGC AGGCGCAGGA GGAGGCCAAG 1200 | | GGCGCGCTGC TGGGGACCAG CGGCCTCAAG CGCCGCTTCA GCCGCCTGGA GAACCGCTAC 1260 | | GAGGCGCTGG CCAAGCAAGT AGCCTCCGAG ATGCGCTTCG TGCAGGACCT CGTGCGCGCC 1320 | | CTGGAGCAGG AGAAGCTGCA GGGCGTGGAG TGCGGGCTGC GCTAG 1365 |
|
| Domain Profile | S: 1 letgkelgsGkfavvrkcrekstGkeyaakflkkrrlkssrrgvsreeierevavlkeir 60 | +e+g+elgsG+fa+vrkcr+k tGkeyaakf+kkrrl+ssrrgvsreeierev++l+eir | Q: 13 YEMGEELGSGQFAIVRKCRQKGTGKEYAAKFIKKRRLSSSRRGVSREEIEREVNILREIR 72 | 5789******************************************************** |
| S: 61 sepnvinlhevyenksdvililelvaGGelfdllaekeslseeeatellkqilegvkylh 120 | +pn+i+lh+++enk+dv+lilelv+GGelfd+laekesl+e+eat++lkqil+gv+ylh | Q: 73 -HPNIITLHDIFENKTDVVLILELVSGGELFDFLAEKESLTEDEATQFLKQILDGVHYLH 131 | .*********************************************************** |
| S: 121 skkivhldlkPenillldkkvpkgdiklidfGlsrkieegaelkeilGtPefvaPeivny 180 | sk+i+h+dlkPeni+lldk+vp+++iklidfG+++kie+g+e+k+i+GtPefvaPeivny | Q: 132 SKRIAHFDLKPENIMLLDKNVPNPRIKLIDFGIAHKIEAGNEFKNIFGTPEFVAPEIVNY 191 | ************************************************************ |
| S: 181 eplsletdmwsiGvityillsgasPflGdtkqetllnisavnldfseelfssvselakdf 240 | epl+le+dmwsiGvityillsgasPflG+tkqetl+nisavn+df+ee+fs++selakdf | Q: 192 EPLGLEADMWSIGVITYILLSGASPFLGETKQETLTNISAVNYDFDEEYFSNTSELAKDF 251 | ************************************************************ |
| S: 241 irrllvkdpekrltieeclehswl 264 | irrllvkdp++r+ti+++lehsw+ | Q: 252 IRRLLVKDPKRRMTIAQSLEHSWI 275 | ***********************8 |
|
Domain Sequence
(FASTA) | | YEMGEELGSG QFAIVRKCRQ KGTGKEYAAK FIKKRRLSSS RRGVSREEIE REVNILREIR 60 | | HPNIITLHDI FENKTDVVLI LELVSGGELF DFLAEKESLT EDEATQFLKQ ILDGVHYLHS 120 | | KRIAHFDLKP ENIMLLDKNV PNPRIKLIDF GIAHKIEAGN EFKNIFGTPE FVAPEIVNYE 180 | | PLGLEADMWS IGVITYILLS GASPFLGETK QETLTNISAV NYDFDEEYFS NTSELAKDFI 240 | | RRLLVKDPKR RMTIAQSLEH SWI 263 |
|
| Keyword | 3D-structure; Activator; Alternative splicing; Apoptosis; ATP-binding; Centromere; Chromatin regulator; Chromosome; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transcription; Transcription regulation; Transferase; Ubl conjugation. |
| Sequence Source | Ensembl |
| Orthology | |
| Gene Ontology | |
| KEGG | |
| InterPros | |
| Pfam | |
| SMARTs | |
| Prosites | |
| Prints | |
| Created Date | 20-Feb-2013 |