EKS-HOS-00297

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00297

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/BUB	387.1	1.8E-115	768	1033	266

Status

Reviewed

Ensembl Protein

ENSP00000441013

UniProt Accession

O43683; O43430; O43643; O60626; Q53QE4;

Protein Name

Mitotic checkpoint serine/threonine-protein kinase BUB1

Protein Synonyms/Alias

hBUB1; BUB1A;

Gene Name

BUB1

Gene Synonyms/Alias

BUB1; BUB1L;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000169679	ENSP00000409713	ENST00000420328
ENSG00000169679	ENSP00000402883	ENST00000447014
ENSG00000169679	ENSP00000441013	ENST00000535254
ENSG00000169679	ENSP00000302530	ENST00000302759
ENSG00000169679	ENSP00000386701	ENST00000409311
ENSG00000169679	ENSP00000392219	ENST00000436916

Organism

Homo sapiens

Functional Description

Serine/threonine-protein kinase that performs 2 crucialfunctions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Plays an important role in defining SGOL1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.

Protein Length

1065

Protein Sequence
(FASTA)

MDTPENVLQY IQWVEENFPE NKEYLITLLE HLMKEFLDKK KYHNDPRFIS YCLKFAEYNS 60

DLHQFFEFLY NHGIGTLSSP LYIAWAGHLE AQGELQHASA VLQRGIQNQA EPREFLQQQY 120

RLFQTRLTET HLPAQARTSE PLHNVQVLNQ MITSKSNPGN NMACISKNQG SELSGVISSA 180

CDKESNMERR VITISKSEYS VHSSLASKVD VEQVVMYCKE KLIRGESEFS FEELRAQKYN 240

QRRKHEQWVN EDRHYMKRKE ANAFEEQLLK QKMDELHKKL HQVVETSHED LPASQERSEV 300

NPARMGPSVG SQQELRAPCL PVTYQQTPVN MEKNPREAPP VVPPLANAIS AALVSPATSQ 360

SIAPPVPLKA QTVTDSMFAV ASKDAGCVNK STHEFKPQSG AEIKEGCETH KVANTSSFHT 420

TPNTSLGMVQ ATPSKVQPSP TVHTKEALGF IMNMFQAPTL PDISDDKDEW QSLDQNEDAF 480

EAQFQKNVRS SGAWGVNKII SSLSSAFHVF EDGNKENYGL PQPKNKPTGA RTFGERSVSR 540

LPSKPKEEVP HAEEFLDDST VWGIRCNKTL APSPKSPGDF TSAAQLASTP FHKLPVESVH 600

ILEDKENVVA KQCTQATLDS CEENMVVPSR DGKFSPIQEK SPKQALSSHM YSASLLRLSQ 660

PAAGGVLTCE AELGVEACRL TDTDAAIAED PPDAIAGLQA EWMQMSSLGT VDAPNFIVGN 720

PWDDKLIFKL LSGLSKPVSS YPNTFEWQCK LPAIKPKTEF QLGSKLVYVH HLLGEGAFAQ 780

VYEATQGDLN DAKNKQKFVL KVQKPANPWE FYIGTQLMER LKPSMQHMFM KFYSAHLFQN 840

GSVLVGELYS YGTLLNAINL YKNTPEKVMP QGLVISFAMR MLYMIEQVHD CEIIHGDIKP 900

DNFILGNGFL EQDDEDDLSA GLALIDLGQS IDMKLFPKGT IFTAKCETSG FQCVEMLSNK 960

PWNYQIDYFG VAATVYCMLF GTYMKVKNEG GECKPEGLFR RLPHLDMWNE FFHVMLNIPD 1020

CHHLPSLDLL RQKLKKVFQQ HYTNKIRALR NRLIVLLLEC KRSRK 1065

Nucleotide Sequence
(FASTA)

ATGGACACCC CGGAAAATGT CCTTCAATAC ATACAGTGGG TAGAAGAGAA TTTTCCTGAG 60

AATAAAGAAT ACTTGATAAC TTTACTAGAA CATTTAATGA AGGAATTTTT AGATAAGAAG 120

AAATACCACA ATGACCCAAG ATTCATCAGT TATTGTTTAA AATTTGCTGA GTACAACAGT 180

GACCTCCATC AATTTTTTGA GTTTCTGTAC AACCATGGGA TTGGAACCCT GTCATCCCCT 240

CTGTACATTG CCTGGGCGGG GCATCTGGAA GCCCAAGGAG AGCTGCAGCA TGCCAGTGCT 300

GTCCTTCAGA GAGGAATTCA AAACCAGGCT GAACCCAGAG AGTTCCTGCA ACAACAATAC 360

AGGTTATTTC AGACACGCCT CACTGAAACC CATTTGCCAG CTCAAGCTAG AACCTCAGAA 420

CCTCTGCATA ATGTTCAGGT TTTAAATCAA ATGATAACAT CAAAATCAAA TCCAGGAAAT 480

AACATGGCCT GCATTTCTAA GAATCAGGGT TCAGAGCTTT CTGGAGTGAT ATCTTCAGCT 540

TGTGATAAAG AGTCAAATAT GGAACGAAGA GTGATCACGA TTTCTAAATC AGAATATTCT 600

GTGCACTCAT CTTTGGCATC CAAAGTTGAT GTTGAGCAGG TTGTTATGTA TTGCAAGGAG 660

AAGCTTATTC GTGGGGAATC AGAATTTTCC TTTGAAGAAT TGAGAGCCCA GAAATACAAT 720

CAACGGAGAA AGCATGAGCA ATGGGTAAAT GAAGACAGAC ATTATATGAA AAGGAAAGAA 780

GCAAATGCTT TTGAAGAACA GCTATTAAAA CAGAAAATGG ATGAACTTCA TAAGAAGTTG 840

CATCAGGTGG TGGAGACATC CCATGAGGAT CTGCCCGCTT CCCAGGAAAG GTCCGAGGTT 900

AATCCAGCAC GTATGGGGCC AAGTGTAGGC TCCCAGCAGG AACTGAGAGC GCCATGTCTT 960

CCAGTAACCT ATCAGCAGAC ACCAGTGAAC ATGGAAAAGA ACCCAAGAGA GGCACCTCCT 1020

GTTGTTCCTC CTTTGGCAAA TGCTATTTCT GCAGCTTTGG TGTCCCCAGC CACCAGCCAG 1080

AGCATTGCTC CTCCTGTTCC TTTGAAAGCC CAGACAGTAA CAGACTCCAT GTTTGCAGTG 1140

GCCAGCAAAG ATGCTGGATG TGTGAATAAG AGTACTCATG AATTCAAGCC ACAGAGTGGA 1200

GCAGAGATCA AAGAAGGGTG TGAAACACAT AAGGTTGCCA ACACAAGTTC TTTTCACACA 1260

ACTCCAAACA CATCACTGGG AATGGTTCAG GCAACGCCAT CCAAAGTGCA GCCATCACCC 1320

ACCGTGCACA CAAAAGAAGC ATTAGGTTTC ATCATGAATA TGTTTCAGGC TCCTACACTT 1380

CCTGATATTT CTGATGACAA AGATGAATGG CAATCTCTAG ATCAAAATGA AGATGCATTT 1440

GAAGCCCAGT TTCAAAAAAA TGTAAGGTCA TCTGGGGCTT GGGGAGTCAA TAAGATCATC 1500

TCTTCTTTGT CATCTGCTTT TCATGTGTTT GAAGATGGAA ACAAAGAAAA TTATGGATTA 1560

CCACAGCCTA AAAATAAACC CACAGGAGCC AGGACCTTTG GAGAACGCTC TGTCAGCAGA 1620

CTTCCTTCAA AACCAAAGGA GGAAGTGCCT CATGCTGAAG AGTTTTTGGA TGACTCAACT 1680

GTATGGGGTA TTCGCTGCAA CAAAACCCTG GCACCCAGTC CTAAGAGCCC AGGAGACTTC 1740

ACATCTGCTG CACAACTTGC GTCTACACCA TTCCACAAGC TTCCAGTGGA GTCAGTGCAC 1800

ATTTTAGAAG ATAAAGAAAA TGTGGTAGCA AAACAGTGTA CCCAGGCGAC TTTGGATTCT 1860

TGTGAGGAAA ACATGGTGGT GCCTTCAAGG GATGGAAAAT TCAGTCCAAT TCAAGAGAAA 1920

AGCCCAAAAC AGGCCTTGTC GTCTCACATG TATTCAGCAT CCTTACTTCG TCTGAGCCAG 1980

CCTGCTGCAG GTGGGGTACT TACCTGTGAG GCAGAGTTGG GCGTTGAGGC TTGCAGACTC 2040

ACAGACACTG ACGCTGCCAT TGCAGAAGAT CCACCAGATG CTATTGCTGG GCTCCAAGCA 2100

GAATGGATGC AGATGAGTTC ACTTGGGACT GTTGATGCTC CAAACTTCAT TGTTGGGAAC 2160

CCATGGGATG ATAAGCTGAT TTTCAAACTT TTATCTGGGC TTTCTAAACC AGTGAGTTCC 2220

TATCCAAATA CTTTTGAATG GCAATGTAAA CTTCCAGCCA TCAAGCCCAA GACTGAATTT 2280

CAATTGGGTT CTAAGCTGGT CTATGTCCAT CACCTTCTTG GAGAAGGAGC CTTTGCCCAG 2340

GTGTACGAAG CTACCCAGGG AGATCTGAAT GATGCTAAAA ATAAACAGAA ATTTGTTTTA 2400

AAGGTCCAAA AGCCTGCCAA CCCCTGGGAA TTCTACATTG GGACCCAGTT GATGGAAAGA 2460

CTAAAGCCAT CTATGCAGCA CATGTTTATG AAGTTCTATT CTGCCCACTT ATTCCAGAAT 2520

GGCAGTGTAT TAGTAGGAGA GCTCTACAGC TATGGAACAT TATTAAATGC CATTAACCTC 2580

TATAAAAATA CCCCTGAAAA AGTGATGCCT CAAGGTCTTG TCATCTCTTT TGCTATGAGA 2640

ATGCTTTACA TGATTGAGCA AGTGCATGAC TGTGAAATCA TTCATGGAGA CATTAAACCA 2700

GACAATTTCA TACTTGGAAA CGGATTTTTG GAACAGGATG ATGAAGATGA TTTATCTGCT 2760

GGCTTGGCAC TGATTGACCT GGGTCAGAGT ATAGATATGA AACTTTTTCC AAAAGGAACT 2820

ATATTCACAG CAAAGTGTGA AACATCTGGT TTTCAGTGTG TTGAGATGCT CAGCAACAAA 2880

CCATGGAACT ACCAGATCGA TTACTTTGGG GTTGCTGCAA CAGTATATTG CATGCTCTTT 2940

GGCACTTACA TGAAAGTGAA AAATGAAGGA GGAGAGTGTA AGCCTGAAGG TCTTTTTAGA 3000

AGGCTTCCTC ATTTGGATAT GTGGAATGAA TTTTTTCATG TTATGTTGAA TATTCCAGAT 3060

TGTCATCATC TTCCATCTTT GGATTTGTTA AGGCAAAAGC TGAAGAAAGT ATTTCAACAA 3120

CACTATACTA ACAAGATTAG GGCCCTACGT AATAGGCTAA TTGTACTGCT CTTAGAATGT 3180

AAGCGTTCAC GAAAATAA 3198

Domain Profile

S: 2     eiekeigeGayakvykaeh....dsksgelvalKvekpansWefYIclqlkeRlkeeell  57

         ++++++geGa+a+vy+a++    d+k++++++lKv+kpan+WefYI +ql+eRlk ++++

Q: 768   YVHHLLGEGAFAQVYEATQgdlnDAKNKQKFVLKVQKPANPWEFYIGTQLMERLK-PSMQ  826

         79******************9999*******************************.77**

S: 58    ksiieissallfqdasllvleyasygtlldlinkvkevtnkvmdeelvvllivqllkive  117

         +++++++sa+lfq++s+lv e++sygtll++in++k++ +kvm++ lv+++++++l+++e

Q: 827   HMFMKFYSAHLFQNGSVLVGELYSYGTLLNAINLYKNTPEKVMPQGLVISFAMRMLYMIE  886

         ************************************************************

S: 118   klHeveIiHgDlkPdnfllgnrimdkadkdkeekglkliDfgrsiDmkllpdgtkfkgka  177

         ++H++eIiHgD+kPdnf+lgn + ++ d+d+ ++gl+liD+g+siDmkl+p+gt f++k+

Q: 887   QVHDCEIIHGDIKPDNFILGNGFLEQDDEDDLSAGLALIDLGQSIDMKLFPKGTIFTAKC  946

         ************************************************************

S: 178   ktdafeciemlagkswsYqvDlfglaavihvllfgkyleviqeggswklkqkikrllkke  237

         +t++f+c+eml++k+w+Yq+D+fg+aa+++++lfg+y++v++egg++k++  ++rl++ +

Q: 947   ETSGFQCVEMLSNKPWNYQIDYFGVAATVYCMLFGTYMKVKNEGGECKPEGLFRRLPHLD  1006

         ************************************************************

S: 238   lwekiferlLNisdastlpsleelveek  265

         +w+++f+++LNi+d+++lpsl+ l++ k

Q: 1007  MWNEFFHVMLNIPDCHHLPSLDLLRQ-K  1033

         ************************95.4

Domain Sequence
(FASTA)

YVHHLLGEGA FAQVYEATQG DLNDAKNKQK FVLKVQKPAN PWEFYIGTQL MERLKPSMQH 60

MFMKFYSAHL FQNGSVLVGE LYSYGTLLNA INLYKNTPEK VMPQGLVISF AMRMLYMIEQ 120

VHDCEIIHGD IKPDNFILGN GFLEQDDEDD LSAGLALIDL GQSIDMKLFP KGTIFTAKCE 180

TSGFQCVEML SNKPWNYQID YFGVAATVYC MLFGTYMKVK NEGGECKPEG LFRRLPHLDM 240

WNEFFHVMLN IPDCHHLPSL DLLRQK 266

Keyword

3D-structure; Apoptosis; ATP-binding; Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition; Complete proteome; Host-virus interaction; Kinase; Kinetochore; Mitosis; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0000777	; C:condensed chromosome kinetochore
GO:0000780	; C:condensed nuclear chromosome, centromeric region
GO:0005829	; C:cytosol
GO:0005524	; F:ATP binding
GO:0004672	; F:protein kinase activity
GO:0004674	; F:protein serine/threonine kinase activity
GO:0006915	; P:apoptotic process
GO:0051301	; P:cell division
GO:0008283	; P:cell proliferation
GO:0007059	; P:chromosome segregation
GO:0007094	; P:mitotic cell cycle spindle assembly checkpoint
GO:0000236	; P:mitotic prometaphase
GO:0007063	; P:regulation of sister chromatid cohesion
GO:0019048	; P:virus-host interaction

KEGG

hsa:699

;

InterPros

IPR011009	; Kinase-like_dom.
IPR013212	; Mad3_BUB1_I.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR008271	; Ser/Thr_kinase_AS.
IPR015661	; Ser/Thr_kinase_Bub1.

Pfam

PF08311	; Mad3_BUB1_I; 1.
PF00069	; Pkinase; 1.

SMARTs

SM00777

; Mad3_BUB1_I; 1.

Prosites

PS51489	; BUB1_N; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013