EPS-HOS-00020

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EPS-HOS-00020

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
PPM/PP2C	275.2	3.4E-81	61	373	313

Status

Reviewed

Ensembl Protein

ENSP00000376720

UniProt Accession

O15297; Q53XP4;

Protein Name

Protein phosphatase 1D

Protein Synonyms/Alias

Protein phosphatase 2C isoform delta; PP2C-delta; Protein phosphatase magnesium-dependent 1 delta; p53-induced protein phosphatase 1;

Gene Name

PPM1D

Gene Synonyms/Alias

PPM1D; WIP1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000170836	ENSP00000465168	ENST00000590418
ENSG00000170836	ENSP00000376720	ENST00000392995
ENSG00000170836	ENSP00000306682	ENST00000305921

Organism

Homo sapiens

Functional Description

Required for the relief of p53-dependent checkpointmediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins.

Protein Length

430

Protein Sequence
(FASTA)

MAGLYSLGVS VFSDQGGRKY MEDVTQIVVE PEPTAEEKPS PRRSLSQPLP PRPSPAALPG 60

GEVSGKGPAV AAREARDPLP DAGASPAPSR CCRRRSSVAF FAVCDGHGGR EAAQFAREHL 120

WGFIKKQKGF TSSEPAKVCA AIRKGFLACH LAMWKKLAEW PKTMTGLPST SGTTASVVII 180

RGMKMYVAHV GDSGVVLGIQ DDPKDDFVRA VEVTQDHKPE LPKERERIEG LGGSVMNKSG 240

VNRVVWKRPR LTHNGPVRRS TVIDQIPFLA VARALGDLWS YDFFSGEFVV SPEPDTSVHT 300

LDPQKHKYII LGSDGLWNMI PPQDAISMCQ DQEEKKYLMG EHGQSCAKML VNRALGRWRQ 360

RMLRADNTSA IVICISPEVD NQGNFTNEDE LYLNLTDSPS YNSQETCVMT PSPCSTPPVK 420

DFGFELDSRK 430

Nucleotide Sequence
(FASTA)

ATGGCGGGGC TGTACTCGCT GGGAGTGAGC GTCTTCTCCG ACCAGGGCGG GAGGAAGTAC 60

ATGGAGGACG TTACTCAAAT CGTTGTGGAG CCCGAACCGA CGGCTGAAGA AAAGCCCTCG 120

CCGCGGCGGT CGCTGTCTCA GCCGTTGCCT CCGCGGCCGT CGCCGGCCGC CCTTCCCGGC 180

GGCGAAGTCT CGGGGAAAGG CCCAGCGGTG GCAGCCCGAG AGGCTCGCGA CCCTCTCCCG 240

GACGCCGGGG CCTCGCCGGC ACCTAGCCGC TGCTGCCGCC GCCGTTCCTC CGTGGCCTTT 300

TTCGCCGTGT GCGACGGGCA CGGCGGGCGG GAGGCGGCAC AGTTTGCCCG GGAGCACTTG 360

TGGGGTTTCA TCAAGAAGCA GAAGGGTTTC ACCTCGTCCG AGCCGGCTAA GGTTTGCGCT 420

GCCATCCGCA AAGGCTTTCT CGCTTGTCAC CTTGCCATGT GGAAGAAACT GGCGGAATGG 480

CCAAAGACTA TGACGGGTCT TCCTAGCACA TCAGGGACAA CTGCCAGTGT GGTCATCATT 540

CGGGGCATGA AGATGTATGT AGCTCACGTA GGTGACTCAG GGGTGGTTCT TGGAATTCAG 600

GATGACCCGA AGGATGACTT TGTCAGAGCT GTGGAGGTGA CACAGGACCA TAAGCCAGAA 660

CTTCCCAAGG AAAGAGAACG AATCGAAGGA CTTGGTGGGA GTGTAATGAA CAAGTCTGGG 720

GTGAATCGTG TAGTTTGGAA ACGACCTCGA CTCACTCACA ATGGACCTGT TAGAAGGAGC 780

ACAGTTATTG ACCAGATTCC TTTTCTGGCA GTAGCAAGAG CACTTGGTGA TTTGTGGAGC 840

TATGATTTCT TCAGTGGTGA ATTTGTGGTG TCACCTGAAC CAGACACAAG TGTCCACACT 900

CTTGACCCTC AGAAGCACAA GTATATTATA TTGGGGAGTG ATGGACTTTG GAATATGATT 960

CCACCACAAG ATGCCATCTC AATGTGCCAG GACCAAGAGG AGAAAAAATA CCTGATGGGT 1020

GAGCATGGAC AATCTTGTGC CAAAATGCTT GTGAATCGAG CATTGGGCCG CTGGAGGCAG 1080

CGTATGCTCC GAGCAGATAA CACTAGTGCC ATAGTAATCT GCATCTCTCC AGAAGTGGAC 1140

AATCAGGGAA ACTTTACCAA TGAAGATGAG TTATACCTGA ACCTGACTGA CAGCCCTTCC 1200

TATAATAGTC AAGAAACCTG TGTGATGACT CCTTCCCCAT GTTCTACACC ACCAGTCAAG 1260

GATTTTGGAT TTGAACTCGA TTCAAGAAAG TGA 1293

Domain Profile

S: 4     gvvsvngrrnemedavtsspnflkspekkdr......laffgvyDGhggsevakfcsekl  57

         g vs++g+    ++a+++ p++++sp+ +++      +aff+v+DGhgg+e+a+f++e+l

Q: 61    GEVSGKGPAVAAREARDPLPDAGASPAPSRCcrrrssVAFFAVCDGHGGREAAQFAREHL  120

         679999***********************99*****************************

S: 58    heilaeelesseseerkvkkalakaFlstdeel.....kvleeksldidksGstavvali  112

         + ++++++ +++se +kv++a++k+Fl+++ ++     +++++++  +++sG+ta+v++i

Q: 121   WGFIKKQKGFTSSEPAKVCAAIRKGFLACHLAMwkklaEWPKTMTGLPSTSGTTASVVII  180

         ************************************************************

S: 113   kdsklyvanvGdSravLcrngk.......avelssdHkpsredEleRieeaggsvilkdg  165

         +++k+yva+vGdS++vL+ +++       ave+++dHkp+ ++E+eRie  ggsv++k+g

Q: 181   RGMKMYVAHVGDSGVVLGIQDDpkddfvrAVEVTQDHKPELPKERERIEGLGGSVMNKSG  240

         *******************77656999999******************************

S: 166   a.Rv.....................lGvLavsRalGdlylk......pyvtpePevtshr  197

         + Rv                     +++Lav+RalGdl+++      ++v+peP++++h+

Q: 241   VnRVvwkrprlthngpvrrstvidqIPFLAVARALGDLWSYdffsgeFVVSPEPDTSVHT  300

         ************************************************************

S: 198   ltsede.fliLasDGlwdvvsneeavslvrdvle....kkeaeqeaakkLvklalargsk  252

         l+++++ ++iL+sDGlw+++++++a+s+++d++e    ++e+ q++ak+Lv++al r+++

Q: 301   LDPQKHkYIILGSDGLWNMIPPQDAISMCQDQEEkkylMGEHGQSCAKMLVNRALGRWRQ  360

         ************************************************************

S: 253   .....dnvsaivv  260

              dn+saiv+

Q: 361   rmlraDNTSAIVI  373

         ***********97

Domain Sequence
(FASTA)

GEVSGKGPAV AAREARDPLP DAGASPAPSR CCRRRSSVAF FAVCDGHGGR EAAQFAREHL 60

WGFIKKQKGF TSSEPAKVCA AIRKGFLACH LAMWKKLAEW PKTMTGLPST SGTTASVVII 120

RGMKMYVAHV GDSGVVLGIQ DDPKDDFVRA VEVTQDHKPE LPKERERIEG LGGSVMNKSG 180

VNRVVWKRPR LTHNGPVRRS TVIDQIPFLA VARALGDLWS YDFFSGEFVV SPEPDTSVHT 240

LDPQKHKYII LGSDGLWNMI PPQDAISMCQ DQEEKKYLMG EHGQSCAKML VNRALGRWRQ 300

RMLRADNTSA IVI 313

Keyword

Cell cycle; Complete proteome; Hydrolase; Magnesium; Manganese; Metal-binding; Phosphoprotein; Protein phosphatase; Reference proteome.

Sequence Source

Ensembl

Orthology

Ortholog group

Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005634	; C:nucleus
GO:0046872	; F:metal ion binding
GO:0004722	; F:protein serine/threonine phosphatase activity
GO:0000086	; P:G2/M transition of mitotic cell cycle
GO:0008285	; P:negative regulation of cell proliferation
GO:0035970	; P:peptidyl-threonine dephosphorylation
GO:0009617	; P:response to bacterium
GO:0009314	; P:response to radiation

KEGG

hsa:8493

;

InterPros

IPR001932	; PP2C-like.
IPR000222	; PP2C_Mn2_Asp60_BS.
IPR015655	; Protein_Pase_2C.

Pfam

PF00481

; PP2C; 1.

SMARTs

SM00332

; PP2Cc; 1.

Prosites

PS01032

; PP2C; 1.

Prints

Created Date

20-Feb-2013