EKS-HOS-00383

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00383

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TKL/MLK	347.5	2.3E-103	119	376	258

Status

Reviewed

Ensembl Protein

ENSP00000309597

UniProt Accession

Q16584; Q53H00; Q59F06; Q6P2G4;

Protein Name

Mitogen-activated protein kinase kinase kinase 11

Protein Synonyms/Alias

Mixed lineage kinase 3; Src-homology 3 domain-containing proline-rich kinase;

Gene Name

MAP3K11

Gene Synonyms/Alias

MAP3K11; MLK3, PTK1, SPRK;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000173327	ENSP00000433886	ENST00000530153
ENSG00000173327	ENSP00000431506	ENST00000524848
ENSG00000173327	ENSP00000435237	ENST00000529839
ENSG00000173327	ENSP00000435970	ENST00000526293
ENSG00000173327	ENSP00000432068	ENST00000532507
ENSG00000173327	ENSP00000309597	ENST00000309100

Organism

Homo sapiens

Functional Description

Activates the JUN N-terminal pathway. Required forserum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.

Protein Length

847

Protein Sequence
(FASTA)

MEPLKSLFLK SPLGSWNGSG SGGGGGGGGG RPEGSPKAAG YANPVWTALF DYEPSGQDEL 60

ALRKGDRVEV LSRDAAISGD EGWWAGQVGG QVGIFPSNYV SRGGGPPPCE VASFQELRLE 120

EVIGIGGFGK VYRGSWRGEL VAVKAARQDP DEDISVTAES VRQEARLFAM LAHPNIIALK 180

AVCLEEPNLC LVMEYAAGGP LSRALAGRRV PPHVLVNWAV QIARGMHYLH CEALVPVIHR 240

DLKSNNILLL QPIESDDMEH KTLKITDFGL AREWHKTTQM SAAGTYAWMA PEVIKASTFS 300

KGSDVWSFGV LLWELLTGEV PYRGIDCLAV AYGVAVNKLT LPIPSTCPEP FAQLMADCWA 360

QDPHRRPDFA SILQQLEALE AQVLREMPRD SFHSMQEGWK REIQGLFDEL RAKEKELLSR 420

EEELTRAARE QRSQAEQLRR REHLLAQWEL EVFERELTLL LQQVDRERPH VRRRRGTFKR 480

SKLRARDGGE RISMPLDFKH RITVQASPGL DRRRNVFEVG PGDSPTFPRF RAIQLEPAEP 540

GQAWGRQSPR RLEDSSNGER RACWAWGPSS PKPGEAQNGR RRSRMDEATW YLDSDDSSPL 600

GSPSTPPALN GNPPRPSLEP EEPKRPVPAE RGSSSGTPKL IQRALLRGTA LLASLGLGRD 660

LQPPGGPGRE RGESPTTPPT PTPAPCPTEP PPSPLICFSL KTPDSPPTPA PLLLDLGIPV 720

GQRSAKSPRR EEEPRGGTVS PPPGTSRSAP GTPGTPRSPP LGLISRPRPS PLRSRIDPWS 780

FVSAGPRPSP LPSPQPAPRR APWTLFPDSD PFWDSPPANP FQGGPQDCRA QTKDMGAQAP 840

WVPEAGP 847

Nucleotide Sequence
(FASTA)

ATGGAGCCCT TGAAGAGCCT CTTCCTCAAG AGCCCTCTAG GGTCATGGAA TGGCAGTGGC 60

AGCGGGGGTG GTGGGGGCGG TGGAGGAGGC CGGCCTGAGG GGTCTCCAAA GGCAGCGGGT 120

TATGCCAACC CGGTGTGGAC AGCCCTGTTC GACTACGAGC CCAGTGGGCA GGATGAGCTG 180

GCCCTGAGGA AGGGTGACCG TGTGGAGGTG CTGTCCCGGG ACGCAGCCAT CTCAGGAGAC 240

GAGGGCTGGT GGGCGGGCCA GGTGGGTGGC CAGGTGGGCA TCTTCCCGTC CAACTATGTG 300

TCTCGGGGTG GCGGCCCGCC CCCCTGCGAG GTGGCCAGCT TCCAGGAGCT GCGGCTGGAG 360

GAGGTGATCG GCATTGGAGG CTTTGGCAAG GTGTACAGGG GCAGCTGGCG AGGTGAGCTG 420

GTGGCTGTGA AGGCAGCTCG CCAGGACCCC GATGAGGACA TCAGTGTGAC AGCCGAGAGC 480

GTTCGCCAGG AGGCCCGGCT CTTCGCCATG CTGGCACACC CCAACATCAT TGCCCTCAAG 540

GCTGTGTGCC TGGAGGAGCC CAACCTGTGC CTGGTGATGG AGTATGCAGC CGGTGGGCCC 600

CTCAGCCGAG CTCTGGCCGG GCGGCGCGTG CCTCCCCATG TGCTGGTCAA CTGGGCTGTG 660

CAGATTGCCC GTGGGATGCA CTACCTGCAC TGCGAGGCCC TGGTGCCCGT CATCCACCGT 720

GATCTCAAGT CCAACAACAT TTTGCTGCTG CAGCCCATTG AGAGTGACGA CATGGAGCAC 780

AAGACCCTGA AGATCACCGA CTTTGGCCTG GCCCGAGAGT GGCACAAAAC CACACAAATG 840

AGTGCCGCGG GCACCTACGC CTGGATGGCT CCTGAGGTTA TCAAGGCCTC CACCTTCTCT 900

AAGGGCAGTG ACGTCTGGAG TTTTGGGGTG CTGCTGTGGG AACTGCTGAC CGGGGAGGTG 960

CCATACCGTG GCATTGACTG CCTTGCTGTG GCCTATGGCG TAGCTGTTAA CAAGCTCACA 1020

CTGCCCATCC CATCCACCTG CCCCGAGCCC TTCGCACAGC TTATGGCCGA CTGCTGGGCG 1080

CAGGACCCCC ACCGCAGGCC CGACTTCGCC TCCATCCTGC AGCAGTTGGA GGCGCTGGAG 1140

GCACAGGTCC TACGGGAAAT GCCGCGGGAC TCCTTCCATT CCATGCAGGA AGGCTGGAAG 1200

CGCGAGATCC AGGGTCTCTT CGACGAGCTG CGAGCCAAGG AAAAGGAACT ACTGAGCCGC 1260

GAGGAGGAGC TGACGCGAGC GGCGCGCGAG CAGCGGTCAC AGGCGGAGCA GCTGCGGCGG 1320

CGCGAGCACC TGCTGGCCCA GTGGGAGCTA GAGGTGTTCG AGCGCGAGCT GACGCTGCTG 1380

CTGCAGCAGG TGGACCGCGA GCGACCGCAC GTGCGCCGCC GCCGCGGGAC ATTCAAGCGC 1440

AGCAAGCTCC GGGCGCGCGA CGGCGGCGAG CGTATCAGCA TGCCACTCGA CTTCAAGCAC 1500

CGCATCACCG TGCAGGCCTC ACCCGGCCTT GACCGGAGGA GAAACGTCTT CGAGGTCGGG 1560

CCTGGGGATT CGCCCACCTT TCCCCGGTTC CGAGCCATCC AGTTGGAGCC TGCAGAGCCA 1620

GGCCAGGCAT GGGGCCGCCA GTCCCCCCGA CGTCTGGAGG ACTCAAGCAA TGGAGAGCGG 1680

CGAGCATGCT GGGCTTGGGG TCCCAGTTCC CCCAAGCCTG GGGAAGCCCA GAATGGGAGG 1740

AGAAGGTCCC GCATGGACGA AGCCACATGG TACCTGGATT CAGATGACTC ATCCCCCTTA 1800

GGATCTCCTT CCACACCCCC AGCACTCAAT GGTAACCCCC CGCGGCCTAG CCTGGAGCCC 1860

GAGGAGCCCA AGAGGCCTGT CCCCGCAGAG CGCGGTAGCA GCTCTGGGAC GCCCAAGCTG 1920

ATCCAGCGGG CGCTGCTGCG CGGCACCGCC CTGCTCGCCT CGCTGGGCCT TGGCCGCGAC 1980

CTGCAGCCGC CGGGAGGCCC AGGACGCGAG CGCGGGGAGT CCCCGACAAC ACCCCCCACG 2040

CCAACGCCCG CGCCCTGCCC GACCGAGCCG CCCCCTTCCC CGCTCATCTG CTTCTCGCTC 2100

AAGACGCCCG ACTCCCCGCC CACTCCTGCA CCCCTGTTGC TGGACCTGGG TATCCCTGTG 2160

GGCCAGCGGT CAGCCAAGAG CCCCCGACGT GAGGAGGAGC CCCGCGGAGG CACTGTCTCA 2220

CCCCCACCGG GGACATCACG CTCTGCTCCT GGCACCCCAG GCACCCCACG TTCACCACCC 2280

CTGGGCCTCA TCAGCCGACC TCGGCCCTCG CCCCTTCGCA GCCGCATTGA TCCCTGGAGC 2340

TTTGTGTCAG CTGGGCCACG GCCTTCTCCC CTGCCATCAC CACAGCCTGC ACCCCGCCGA 2400

GCACCCTGGA CCTTGTTCCC GGACTCAGAC CCCTTCTGGG ACTCCCCACC TGCCAACCCC 2460

TTCCAGGGGG GCCCCCAGGA CTGCAGGGCA CAGACCAAAG ACATGGGTGC CCAGGCCCCG 2520

TGGGTGCCGG AAGCGGGGCC TTGA 2544

Domain Profile

S: 3     leeligsgafgkvykakwrkkevavKklreeaee..kekleklereakllsklshpniie  60

         lee+ig g fgkvy+++wr++ vavK++r++  e  + ++e++++ea+l+++l+hpnii+

Q: 119   LEEVIGIGGFGKVYRGSWRGELVAVKAARQDPDEdiSVTAESVRQEARLFAMLAHPNIIA  178

         789****************************99988999*********************

S: 61    llGvcleepnlllvmeyveggsLsnvlhekskreidlevlvswalqiArGmaYLhseapk  120

         l++vcleepnl+lvmey++gg Ls++l++   r+++++vlv+wa+qiArGm+YLh ea +

Q: 179   LKAVCLEEPNLCLVMEYAAGGPLSRALAG---RRVPPHVLVNWAVQIARGMHYLHCEALV  235

         *****************************...9***************************

S: 121   kiihrdLksknvLl.......seekktlkicdFGtarelskstkmsaagtlawmaPEvlk  173

         ++ihrdLks+n+Ll       ++e+ktlki+dFG are++k+t+msaagt+awmaPEv+k

Q: 236   PVIHRDLKSNNILLlqpiesdDMEHKTLKITDFGLAREWHKTTQMSAAGTYAWMAPEVIK  295

         ********************999*************************************

S: 174   eekvseksDvwSygvvLWelltrevPykdlselaiaykvalkglrlpipsscpeklkkLm  233

         ++++s+ sDvwS+gv+LWellt+evPy++++ la+ay+va+++l+lpips+cpe++++Lm

Q: 296   ASTFSKGSDVWSFGVLLWELLTGEVPYRGIDCLAVAYGVAVNKLTLPIPSTCPEPFAQLM  355

         ************************************************************

S: 234   kecwnedpekRpsfseivksl  254

          +cw++dp++Rp+f++i+++l

Q: 356   ADCWAQDPHRRPDFASILQQL  376

         *****************9986

Domain Sequence
(FASTA)

LEEVIGIGGF GKVYRGSWRG ELVAVKAARQ DPDEDISVTA ESVRQEARLF AMLAHPNIIA 60

LKAVCLEEPN LCLVMEYAAG GPLSRALAGR RVPPHVLVNW AVQIARGMHY LHCEALVPVI 120

HRDLKSNNIL LLQPIESDDM EHKTLKITDF GLAREWHKTT QMSAAGTYAW MAPEVIKAST 180

FSKGSDVWSF GVLLWELLTG EVPYRGIDCL AVAYGVAVNK LTLPIPSTCP EPFAQLMADC 240

WAQDPHRRPD FASILQQL 258

Keyword

ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Serine/threonine-protein kinase; SH3 domain; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Dipodomys ordii"; ?>Equus caballus"; ?>Felis catus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005813	; C:centrosome
GO:0005874	; C:microtubule
GO:0005524	; F:ATP binding
GO:0004706	; F:JUN kinase kinase kinase activity
GO:0004713	; F:protein tyrosine kinase activity
GO:0007257	; P:activation of JUN kinase activity
GO:0008219	; P:cell death
GO:0008283	; P:cell proliferation
GO:0000080	; P:G1 phase of mitotic cell cycle
GO:0007017	; P:microtubule-based process
GO:0046330	; P:positive regulation of JNK cascade
GO:0043525	; P:positive regulation of neuron apoptotic process
GO:0046777	; P:protein autophosphorylation

KEGG

hsa:4296

;

InterPros

IPR011009	; Kinase-like_dom.
IPR016231	; MAPKKK9/10/11.
IPR015785	; MAPKKK9/10/11-like.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008271	; Ser/Thr_kinase_AS.
IPR011511	; SH3_2.
IPR001452	; SH3_domain.
IPR020635	; Tyr_kinase_cat_dom.

Pfam

PF07714	; Pkinase_Tyr; 1.
PF07653	; SH3_2; 1.

SMARTs

SM00326	; SH3; 1.
SM00219	; TyrKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS50002	; SH3; 1.

Prints

PR00452	; SH3DOMAIN.
PR00109	; TYRKINASE.

Created Date

20-Feb-2013