EKS-HOS-00269

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00269

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/PLK	416.7	1.8E-124	62	314	253

Status

Reviewed

Ensembl Protein

ENSP00000361275

UniProt Accession

Q9H4B4; Q15767; Q5JR99; Q96CV1;

Protein Name

Serine/threonine-protein kinase PLK3

Protein Synonyms/Alias

Cytokine-inducible serine/threonine-protein kinase; FGF-inducible kinase; Polo-like kinase 3; PLK-3; Proliferation-related kinase;

Gene Name

PLK3

Gene Synonyms/Alias

PLK3; CNK, FNK, PRK;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000173846	ENSP00000361275	ENST00000372201

Organism

Homo sapiens

Functional Description

Serine/threonine-protein kinase involved in cell cycleregulation, response to stress and Golgi disassembly. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates ATF2, BCL2L1, CDC25A, CDC25C, CHEK2, HIF1A, JUN, p53/TP53, p73/TP73, PTEN, TOP2A and VRK1. Involved in cell cycle regulation: required for entry into S phase and cytokinesis. Phosphorylates BCL2L1, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Plays a key role in response to stress: rapidly activated upon stress stimulation, such as ionizing radiation, reactive oxygen species (ROS), hyperosmotic stress, UV irradiation and hypoxia. Involved in DNA damage response and G1/S transition checkpoint by phosphorylating CDC25A, p53/TP53 and p73/TP73. Phosphorylates p53/TP53 in response to reactive oxygen species (ROS), thereby promoting p53/TP53-mediated apoptosis. Phosphorylates CHEK2 in response to DNA damage, promoting the G2/M transition checkpoint. Phosphorylates the transcription factor p73/TP73 in response to DNA damage, leading to inhibit p73/TP73-mediated transcriptional activation and pro-apoptotic functions. Phosphorylates HIF1A and JUN is response to hypoxia. Phosphorylates ATF2 following hyperosmotic stress in corneal epithelium. Also involved in Golgi disassembly during the cell cycle: part of a MEK1/MAP2K1-dependent pathway that induces Golgi fragmentation during mitosis by mediating phosphorylation of VRK1. May participate in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation, via its interaction with CIB1.

Protein Length

646

Protein Sequence
(FASTA)

MEPAAGFLSP RPFQRAAAAP APPAGPGPPP SALRGPELEM LAGLPTSDPG RLITDPRSGR 60

TYLKGRLLGK GGFARCYEAT DTETGSAYAV KVIPQSRVAK PHQREKILNE IELHRDLQHR 120

HIVRFSHHFE DADNIYIFLE LCSRKSLAHI WKARHTLLEP EVRYYLRQIL SGLKYLHQRG 180

ILHRDLKLGN FFITENMELK VGDFGLAARL EPPEQRKKTI CGTPNYVAPE VLLRQGHGPE 240

ADVWSLGCVM YTLLCGSPPF ETADLKETYR CIKQVHYTLP ASLSLPARQL LAAILRASPR 300

DRPSIDQILR HDFFTKGYTP DRLPISSCVT VPDLTPPNPA RSLFAKVTKS LFGRKKKSKN 360

HAQERDEVSG LVSGLMRTSV GHQDARPEAP AASGPAPVSL VETAPEDSSP RGTLASSGDG 420

FEEGLTVATV VESALCALRN CIAFMPPAEQ NPAPLAQPEP LVWVSKWVDY SNKFGFGYQL 480

SSRRVAVLFN DGTHMALSAN RKTVHYNPTS TKHFSFSVGA VPRALQPQLG ILRYFASYME 540

QHLMKGGDLP SVEEVEVPAP PLLLQWVKTD QALLMLFSDG TVQVNFYGDH TKLILSGWEP 600

LLVTFVARNR SACTYLASHL RQLGCSPDLR QRLRYALRLL RDRSPA 646

Nucleotide Sequence
(FASTA)

ATGGAGCCTG CCGCCGGTTT CCTGTCTCCG CGCCCCTTCC AGCGTGCGGC CGCCGCGCCC 60

GCTCCCCCGG CCGGGCCCGG GCCGCCTCCG AGTGCCTTGC GCGGACCTGA GCTGGAGATG 120

CTGGCCGGGC TACCGACGTC AGACCCCGGG CGCCTCATCA CGGACCCGCG CAGCGGCCGC 180

ACCTACCTCA AAGGCCGCTT GTTGGGCAAG GGGGGCTTCG CCCGCTGCTA CGAGGCCACT 240

GACACAGAGA CTGGCAGCGC CTACGCTGTC AAAGTCATCC CGCAGAGCCG CGTCGCCAAG 300

CCGCATCAGC GCGAGAAGAT CCTAAATGAG ATTGAGCTGC ACCGAGACCT GCAGCACCGC 360

CACATCGTGC GTTTTTCGCA CCACTTTGAG GACGCTGACA ACATCTACAT TTTCTTGGAG 420

CTCTGCAGCC GAAAGTCCCT GGCCCACATC TGGAAGGCCC GGCACACCCT GTTGGAGCCA 480

GAAGTGCGCT ACTACCTGCG GCAGATCCTT TCTGGCCTCA AGTACTTGCA CCAGCGCGGC 540

ATCTTGCACC GGGACCTCAA GTTGGGAAAT TTTTTCATCA CTGAGAACAT GGAACTGAAG 600

GTGGGGGATT TTGGGCTGGC AGCCCGGTTG GAGCCTCCGG AGCAGAGGAA GAAGACCATC 660

TGTGGCACCC CCAACTATGT GGCTCCAGAA GTGCTGCTGA GACAGGGCCA CGGCCCTGAG 720

GCGGATGTAT GGTCACTGGG CTGTGTCATG TACACGCTGC TCTGCGGGAG CCCTCCCTTT 780

GAGACGGCTG ACCTGAAGGA GACGTACCGC TGCATCAAGC AGGTTCACTA CACGCTGCCT 840

GCCAGCCTCT CACTGCCTGC CCGGCAGCTC CTGGCCGCCA TCCTTCGGGC CTCACCCCGA 900

GACCGCCCCT CTATTGACCA GATCCTGCGC CATGACTTCT TTACCAAGGG CTACACCCCC 960

GATCGACTCC CTATCAGCAG CTGCGTGACA GTCCCAGACC TGACACCCCC CAACCCAGCT 1020

AGGAGTCTGT TTGCCAAAGT TACCAAGAGC CTCTTTGGCA GAAAGAAGAA GAGTAAGAAT 1080

CATGCCCAGG AGAGGGATGA GGTCTCCGGT TTGGTGAGCG GCCTCATGCG CACATCCGTT 1140

GGCCATCAGG ATGCCAGGCC AGAGGCTCCA GCAGCTTCTG GCCCAGCCCC TGTCAGCCTG 1200

GTAGAGACAG CACCTGAAGA CAGCTCACCC CGTGGGACAC TGGCAAGCAG TGGAGATGGA 1260

TTTGAAGAAG GTCTGACTGT GGCCACAGTA GTGGAGTCAG CCCTTTGTGC TCTGAGAAAT 1320

TGTATAGCCT TCATGCCCCC AGCGGAACAG AACCCGGCCC CCCTGGCCCA GCCAGAGCCT 1380

CTGGTGTGGG TCAGCAAGTG GGTTGACTAC TCCAATAAGT TCGGCTTTGG GTATCAACTG 1440

TCCAGCCGCC GTGTGGCTGT GCTCTTCAAC GATGGCACAC ATATGGCCCT GTCGGCCAAC 1500

AGAAAGACTG TGCACTACAA TCCCACCAGC ACAAAGCACT TCTCCTTCTC CGTGGGTGCT 1560

GTGCCCCGGG CCCTGCAGCC TCAGCTGGGT ATCCTGCGGT ACTTCGCCTC CTACATGGAG 1620

CAGCACCTCA TGAAGGGTGG AGATCTGCCC AGTGTGGAAG AGGTAGAGGT ACCTGCTCCG 1680

CCCTTGCTGC TGCAGTGGGT CAAGACGGAT CAGGCTCTCC TCATGCTGTT TAGTGATGGC 1740

ACTGTCCAGG TGAACTTCTA CGGGGACCAC ACCAAGCTGA TTCTCAGTGG CTGGGAGCCC 1800

CTCCTTGTGA CTTTTGTGGC CCGAAATCGT AGTGCTTGTA CTTACCTCGC TTCCCACCTT 1860

CGGCAGCTGG GCTGCTCTCC AGACCTGCGG CAGCGACTCC GCTATGCTCT GCGCCTGCTC 1920

CGGGACCGCA GCCCAGCCTA G 1941

Domain Profile

S: 1     yergkllGkGgfakcyelkdletkevfavkvvpksllakekqrekvekeieihrslkhkn  60

         y +g+llGkGgfa+cye++d+et++++avkv+p+s++ak++qrek+ +eie+hr+l+h++

Q: 62    YLKGRLLGKGGFARCYEATDTETGSAYAVKVIPQSRVAKPHQREKILNEIELHRDLQHRH  121

         889*********************************************************

S: 61    ivklyeffedkenvylvlelcsrrslaellkrrkaltepevryylrqivsglkylhekri  120

         iv+++++fed++n+y++lelcsr+sla+++k+r++l epevryylrqi+sglkylh++ i

Q: 122   IVRFSHHFEDADNIYIFLELCSRKSLAHIWKARHTLLEPEVRYYLRQILSGLKYLHQRGI  181

         ************************************************************

S: 121   lhrdlklgnlflneelevkigdfGlatrlekeeerkktlcGtPnyiaPevlskkghslev  180

         lhrdlklgn+f++e++e+k+gdfGla+rle++e+rkkt+cGtPny+aPevl ++gh+ e+

Q: 182   LHRDLKLGNFFITENMELKVGDFGLAARLEPPEQRKKTICGTPNYVAPEVLLRQGHGPEA  241

         ************************************************************

S: 181   diWslGcilytllvGkPPfetkelketyekikkaeyslPsslskeaksliakllkkePee  240

         d+WslGc++ytll+G+PPfet++lkety++ik+ +y+lP+sls +a++l+a++l+++P++

Q: 242   DVWSLGCVMYTLLCGSPPFETADLKETYRCIKQVHYTLPASLSLPARQLLAAILRASPRD  301

         ************************************************************

S: 241   rpsleevlkdefl  253

         rps++++l+++f+

Q: 302   RPSIDQILRHDFF  314

         ************8

Domain Sequence
(FASTA)

YLKGRLLGKG GFARCYEATD TETGSAYAVK VIPQSRVAKP HQREKILNEI ELHRDLQHRH 60

IVRFSHHFED ADNIYIFLEL CSRKSLAHIW KARHTLLEPE VRYYLRQILS GLKYLHQRGI 120

LHRDLKLGNF FITENMELKV GDFGLAARLE PPEQRKKTIC GTPNYVAPEV LLRQGHGPEA 180

DVWSLGCVMY TLLCGSPPFE TADLKETYRC IKQVHYTLPA SLSLPARQLL AAILRASPRD 240

RPSIDQILRH DFF 253

Keyword

Apoptosis; ATP-binding; Cell cycle; Complete proteome; Cytoplasm; Cytoskeleton; DNA damage; Golgi apparatus; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Macropus eugenii"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005813	; C:centrosome
GO:0030425	; C:dendrite
GO:0005795	; C:Golgi stack
GO:0043025	; C:neuronal cell body
GO:0005730	; C:nucleolus
GO:0005524	; F:ATP binding
GO:0002039	; F:p53 binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0006915	; P:apoptotic process
GO:0000910	; P:cytokinesis
GO:0007113	; P:endomitotic cell cycle
GO:0071779	; P:G1/S transition checkpoint
GO:0000082	; P:G1/S transition of mitotic cell cycle
GO:0090166	; P:Golgi disassembly
GO:0071780	; P:mitotic cell cycle G2/M transition checkpoint
GO:0043066	; P:negative regulation of apoptotic process
GO:0000122	; P:negative regulation of transcription from RNA polymerase II promoter
GO:2000777	; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process involved in cellular response to hypoxia
GO:0043491	; P:protein kinase B signaling cascade
GO:0006974	; P:response to DNA damage stimulus
GO:0006970	; P:response to osmotic stress
GO:0009314	; P:response to radiation
GO:0000302	; P:response to reactive oxygen species
GO:0000084	; P:S phase of mitotic cell cycle

KEGG

hsa:1263

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000959	; POLO_box_duplicated_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.
IPR020658	; Ser/Thr_kinase_Plk3.

Pfam

PF00069	; Pkinase; 1.
PF00659	; POLO_box; 2.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS50078	; POLO_BOX; 2.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013