EPS-HOS-00028

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EPS-HOS-00028

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
PPM/PP2C	191.3	1.3E-55	233	486	254

Status

Reviewed

Ensembl Protein

ENSP00000312411

UniProt Accession

Q8WY54; Q68DW1; Q7LAF3; Q9UPT0;

Protein Name

Protein phosphatase 1E

Protein Synonyms/Alias

Ca(2+)/calmodulin-dependent protein kinase phosphatase N; CaMKP-N; CaMKP-nucleus; CaMKN; Partner of PIX 1; Partner of PIX-alpha; Partner of PIXA;

Gene Name

PPM1E

Gene Synonyms/Alias

PPM1E; CAMKN, KIAA1072, POPX1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000175175	ENSP00000312411	ENST00000308249

Organism

Homo sapiens

Functional Description

Protein phosphatase that inactivates multifunctional CaMkinases such as CAMK4 and CAMK2 (By similarity). Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42.

Protein Length

755

Protein Sequence
(FASTA)

MAGCIPEEKT YRRFLELFLG EFRGPCGGGE PEPEPEPEPE PEPESEPEPE PELVEAEAAE 60

ASVEEPGEEA ATVAATEEGD QEQDPEPEEE AAVEGEEEEE GAATAAAAPG HSAVPPPPPQ 120

LPPLPPLPRP LSERITREEV EGESLDLCLQ QLYKYNCPSF LAAALARATS DEVLQSDLSA 180

HYIPKETDGT EGTVEIETVK LARSVFSKLH EICCSWVKDF PLRRRPQLYY ETSIHAIKNM 240

RRKMEDKHVC IPDFNMLFNL EDQEEQAYFA VFDGHGGVDA AIYASIHLHV NLVRQEMFPH 300

DPAEALCRAF RVTDERFVQK AARESLRCGT TGVVTFIRGN MLHVAWVGDS QVMLVRKGQA 360

VELMKPHKPD REDEKQRIEA LGGCVVWFGA WRVNGSLSVS RAIGDAEHKP YICGDADSAS 420

TVLDGTEDYL ILACDGFYDT VNPDEAVKVV SDHLKENNGD SSMVAHKLVA SARDAGSSDN 480

ITVIVVFLRD MNKAVNVSEE SDWTENSFQG GQEDGGDDKE NHGECKRPWP QHQCSAPADL 540

GYDGRVDSFT DRTSLSPGSQ INVLEDPGYL DLTQIEASKP HSAQFLLPVE MFGPGAPKKA 600

NLINELMMEK KSVQSSLPEW SGAGEFPTAF NLGSTGEQIY RMQSLSPVCS GLENEQFKSP 660

GNRVSRLSHL RHHYSKKWHR FRFNPKFYSF LSAQEPSHKI GTSLSSLTGS GKRNRIRSSL 720

PWRQNSWKGY SENMRKLRKT HDIPCPDLPW SYKIE 755

Nucleotide Sequence
(FASTA)

ATGGCCGGCT GCATCCCTGA GGAGAAAACT TACCGGCGCT TCCTGGAGCT ATTCCTGGGC 60

GAGTTTCGCG GACCGTGCGG CGGCGGCGAG CCGGAGCCGG AACCCGAACC CGAACCCGAA 120

CCCGAACCCG AGTCCGAGCC CGAGCCCGAA CCTGAACTGG TAGAAGCTGA GGCGGCCGAG 180

GCTTCGGTAG AGGAACCCGG GGAGGAGGCG GCCACGGTAG CCGCGACGGA GGAGGGGGAC 240

CAGGAGCAAG ACCCGGAGCC CGAGGAGGAG GCGGCGGTTG AGGGTGAGGA GGAGGAGGAG 300

GGCGCGGCGA CGGCGGCGGC AGCCCCGGGG CACTCGGCCG TGCCGCCGCC GCCGCCCCAG 360

CTGCCGCCTT TGCCCCCGCT CCCGCGACCG CTGTCAGAGC GCATCACCCG CGAGGAGGTG 420

GAGGGCGAAA GCCTGGACCT GTGCCTGCAG CAGCTCTACA AATATAATTG CCCTTCCTTT 480

TTGGCTGCTG CTTTAGCCAG AGCCACATCA GATGAAGTCC TTCAGAGTGA TCTTTCTGCA 540

CATTATATCC CAAAGGAAAC GGATGGCACA GAAGGGACTG TGGAGATTGA GACAGTGAAA 600

TTGGCCCGTT CTGTCTTCAG CAAACTACAC GAGATTTGCT GCAGCTGGGT GAAAGACTTC 660

CCCCTCCGCA GGAGACCCCA GCTTTATTAT GAGACATCAA TCCATGCCAT CAAAAACATG 720

CGCAGGAAAA TGGAGGACAA ACATGTCTGC ATTCCTGACT TTAATATGCT CTTCAACCTA 780

GAGGACCAGG AAGAACAAGC TTACTTTGCA GTGTTTGATG GCCATGGGGG AGTAGATGCT 840

GCTATTTATG CCTCCATTCA CCTCCACGTT AACTTAGTCC GCCAGGAGAT GTTCCCCCAT 900

GATCCTGCTG AGGCCCTGTG CAGGGCCTTC CGGGTCACTG ATGAGCGGTT TGTGCAGAAA 960

GCAGCCAGGG AGAGCTTAAG ATGTGGGACC ACAGGAGTGG TGACTTTCAT CAGAGGCAAC 1020

ATGCTACATG TGGCCTGGGT GGGTGATTCC CAGGTTATGC TTGTGAGAAA GGGCCAAGCT 1080

GTTGAACTAA TGAAGCCACA CAAACCAGAC AGAGAGGATG AAAAGCAGAG AATTGAGGCC 1140

CTTGGAGGTT GCGTAGTCTG GTTTGGTGCC TGGAGGGTGA ATGGAAGTCT GTCGGTTTCC 1200

AGAGCTATTG GAGATGCTGA ACATAAGCCA TATATCTGTG GGGATGCAGA TTCTGCCTCC 1260

ACTGTTCTGG ATGGGACCGA AGACTACCTC ATTCTGGCCT GTGATGGCTT CTATGACACC 1320

GTGAACCCTG ATGAGGCAGT GAAAGTTGTG TCCGACCACC TGAAAGAGAA TAATGGAGAC 1380

AGCAGCATGG TTGCCCACAA ATTAGTGGCA TCAGCTCGTG ATGCTGGGTC AAGTGATAAC 1440

ATCACGGTTA TTGTGGTATT CCTGAGGGAC ATGAACAAAG CTGTAAATGT TAGTGAGGAA 1500

TCAGATTGGA CAGAGAACTC TTTTCAAGGA GGGCAAGAAG ATGGTGGGGA TGATAAGGAG 1560

AATCATGGAG AGTGCAAACG CCCTTGGCCT CAGCACCAGT GCTCAGCACC AGCCGACCTA 1620

GGCTATGATG GGCGTGTGGA TTCATTCACT GATAGAACTA GCCTGAGCCC AGGGTCCCAA 1680

ATCAACGTGC TGGAAGACCC AGGCTACCTA GATCTCACAC AAATAGAAGC AAGCAAACCT 1740

CACAGTGCCC AGTTTTTGCT ACCAGTTGAG ATGTTTGGTC CTGGTGCACC AAAGAAAGCA 1800

AATCTTATTA ATGAGTTAAT GATGGAGAAA AAATCAGTTC AGTCATCATT GCCTGAATGG 1860

AGTGGTGCTG GAGAGTTTCC CACTGCTTTC AATTTGGGTT CAACAGGGGA GCAGATATAC 1920

AGAATGCAGA GCTTGTCTCC TGTCTGTTCA GGGTTGGAAA ATGAACAGTT CAAATCCCCG 1980

GGAAACAGAG TTTCTAGATT GTCTCATTTA CGCCACCACT ACTCAAAGAA GTGGCACAGA 2040

TTCAGGTTTA ATCCAAAGTT TTATTCATTT CTCTCTGCTC AAGAGCCTTC CCACAAAATA 2100

GGCACTAGCC TGTCCTCACT TACTGGAAGT GGGAAGAGAA ATAGGATAAG AAGTTCTCTG 2160

CCATGGAGGC AAAATAGTTG GAAAGGGTAC AGTGAAAACA TGAGGAAGCT CAGAAAGACT 2220

CATGATATTC CATGCCCAGA TCTTCCTTGG AGCTATAAAA TAGAATAA 2268

Domain Profile

S: 4     gvvsvngrrnemedavtsspnflkspekkdr..laffgvyDGhggsevakfcseklheil  61

         ++ +++++r++med++   p+f    + +d+   a+f+v+DGhgg ++a ++s +lh  l

Q: 233   SIHAIKNMRRKMEDKHVCIPDFNMLFNLEDQeeQAYFAVFDGHGGVDAAIYASIHLHVNL  292

         567899**************99766555544459************************99

S: 62    aeelesseseerkvkkalakaFlstdeelkvleeksldidksGstavvalikdsklyvan  121

          ++         +  +al +aF  tde++  ++++  ++ ++G+t vv  i+++ l+va

Q: 293   VRQEM----FPHDPAEALCRAFRVTDERF--VQKAARESLRCGTTGVVTFIRGNMLHVAW  346

         99955....6788888999**********..7777788888*******************

S: 122   vGdSravLcrngkavelssdHkpsredEleRieeaggsvilkdga.RvlGvLavsRalGd  180

         vGdS+++L r+g+avel + Hkp+redE++Rie+ gg v+ + ga Rv+G+L vsRa+Gd

Q: 347   VGDSQVMLVRKGQAVELMKPHKPDREDEKQRIEALGGCVV-WFGAwRVNGSLSVSRAIGD  405

         *************************************999.55899**************

S: 181   lylkpyvtpePevtshrltsedefliLasDGlwdvvsneeavslvrdvle.kkeaeqeaa  239

          + kpy+  + +  s+ l+  +++liLa+DG +d+v++ eav++v d+l+ ++ + + +a

Q: 406   AEHKPYICGDADSASTVLDGTEDYLILACDGFYDTVNPDEAVKVVSDHLKeNNGDSSMVA  465

         ************************************************9966778899**

S: 240   kkLvklalargskdnvsaivv  260

         +kLv+ a++ gs+dn+++ivv

Q: 466   HKLVASARDAGSSDNITVIVV  486

         *****************9997

Domain Sequence
(FASTA)

SIHAIKNMRR KMEDKHVCIP DFNMLFNLED QEEQAYFAVF DGHGGVDAAI YASIHLHVNL 60

VRQEMFPHDP AEALCRAFRV TDERFVQKAA RESLRCGTTG VVTFIRGNML HVAWVGDSQV 120

MLVRKGQAVE LMKPHKPDRE DEKQRIEALG GCVVWFGAWR VNGSLSVSRA IGDAEHKPYI 180

CGDADSASTV LDGTEDYLIL ACDGFYDTVN PDEAVKVVSD HLKENNGDSS MVAHKLVASA 240

RDAGSSDNIT VIVV 254

Keyword

Alternative splicing; Complete proteome; Cytoplasm; Hydrolase; Magnesium; Manganese; Metal-binding; Nucleus; Polymorphism; Protein phosphatase; Reference proteome; Repeat.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0005730	; C:nucleolus
GO:0043234	; C:protein complex
GO:0046872	; F:metal ion binding
GO:0004722	; F:protein serine/threonine phosphatase activity
GO:0035690	; P:cellular response to drug
GO:0006469	; P:negative regulation of protein kinase activity
GO:0035970	; P:peptidyl-threonine dephosphorylation
GO:0051496	; P:positive regulation of stress fiber assembly

KEGG

InterPros

IPR001932	; PP2C-like.
IPR000222	; PP2C_Mn2_Asp60_BS.
IPR015655	; Protein_Pase_2C.

Pfam

PF00481

; PP2C; 1.

SMARTs

SM00331	; PP2C_SIG; 1.
SM00332	; PP2Cc; 1.

Prosites

PS01032

; PP2C; 1.

Prints

Created Date

20-Feb-2013