EKS-HOS-00373

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00373

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/Src	447.4	6.8E-134	277	526	250

Status

Reviewed

Ensembl Protein

ENSP00000324740

UniProt Accession

P07947; A6NLB3; D3DUH1;

Protein Name

Tyrosine-protein kinase Yes

Protein Synonyms/Alias

Proto-oncogene c-Yes; p61-Yes;

Gene Name

YES1

Gene Synonyms/Alias

YES1; YES;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000176105	ENSP00000462468	ENST00000584307
ENSG00000176105	ENSP00000324740	ENST00000314574
ENSG00000176105	ENSP00000464380	ENST00000577961

Organism

Homo sapiens

Functional Description

Non-receptor protein tyrosine kinase that is involved inthe regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis.

Protein Length

543

Protein Sequence
(FASTA)

MGCIKSKENK SPAIKYRPEN TPEPVSTSVS HYGAEPTTVS PCPSSSAKGT AVNFSSLSMT 60

PFGGSSGVTP FGGASSSFSV VPSSYPAGLT GGVTIFVALY DYEARTTEDL SFKKGERFQI 120

INNTEGDWWE ARSIATGKNG YIPSNYVAPA DSIQAEEWYF GKMGRKDAER LLLNPGNQRG 180

IFLVRESETT KGAYSLSIRD WDEIRGDNVK HYKIRKLDNG GYYITTRAQF DTLQKLVKHY 240

TEHADGLCHK LTTVCPTVKP QTQGLAKDAW EIPRESLRLE VKLGQGCFGE VWMGTWNGTT 300

KVAIKTLKPG TMMPEAFLQE AQIMKKLRHD KLVPLYAVVS EEPIYIVTEF MSKGSLLDFL 360

KEGDGKYLKL PQLVDMAAQI ADGMAYIERM NYIHRDLRAA NILVGENLVC KIADFGLARL 420

IEDNEYTARQ GAKFPIKWTA PEAALYGRFT IKSDVWSFGI LQTELVTKGR VPYPGMVNRE 480

VLEQVERGYR MPCPQGCPES LHELMNLCWK KDPDERPTFE YIQSFLEDYF TATEPQYQPG 540

ENL 543

Nucleotide Sequence
(FASTA)

ATGGGCTGCA TTAAAAGTAA AGAAAACAAA AGTCCAGCCA TTAAATACAG ACCTGAAAAT 60

ACTCCAGAGC CTGTCAGTAC AAGTGTGAGC CATTATGGAG CAGAACCCAC TACAGTGTCA 120

CCATGTCCGT CATCTTCAGC AAAGGGAACA GCAGTTAATT TCAGCAGTCT TTCCATGACA 180

CCATTTGGAG GATCCTCAGG GGTAACGCCT TTTGGAGGTG CATCTTCCTC ATTTTCAGTG 240

GTGCCAAGTT CATATCCTGC TGGTTTAACA GGTGGTGTTA CTATATTTGT GGCCTTATAT 300

GATTATGAAG CTAGAACTAC AGAAGACCTT TCATTTAAGA AGGGTGAAAG ATTTCAAATA 360

ATTAACAATA CGGAAGGAGA TTGGTGGGAA GCAAGATCAA TCGCTACAGG AAAGAATGGT 420

TATATCCCGA GCAATTATGT AGCGCCTGCA GATTCCATTC AGGCAGAAGA ATGGTATTTT 480

GGCAAAATGG GGAGAAAAGA TGCTGAAAGA TTACTTTTGA ATCCTGGAAA TCAACGAGGT 540

ATTTTCTTAG TAAGAGAGAG TGAAACAACT AAAGGTGCTT ATTCCCTTTC TATTCGTGAT 600

TGGGATGAGA TAAGGGGTGA CAATGTGAAA CACTACAAAA TTAGGAAACT TGACAATGGT 660

GGATACTATA TCACAACCAG AGCACAATTT GATACTCTGC AGAAATTGGT GAAACACTAC 720

ACAGAACATG CTGATGGTTT ATGCCACAAG TTGACAACTG TGTGTCCAAC TGTGAAACCT 780

CAGACTCAAG GTCTAGCAAA AGATGCTTGG GAAATCCCTC GAGAATCTTT GCGACTAGAG 840

GTTAAACTAG GACAAGGATG TTTCGGCGAA GTGTGGATGG GAACATGGAA TGGAACCACG 900

AAAGTAGCAA TCAAAACACT AAAACCAGGT ACAATGATGC CAGAAGCTTT CCTTCAAGAA 960

GCTCAGATAA TGAAAAAATT AAGACATGAT AAACTTGTTC CACTATATGC TGTTGTTTCT 1020

GAAGAACCAA TTTACATTGT CACTGAATTT ATGTCAAAAG GAAGCTTATT AGATTTCCTT 1080

AAGGAAGGAG ATGGAAAGTA TTTGAAGCTT CCACAGCTGG TTGATATGGC TGCTCAGATT 1140

GCTGATGGTA TGGCATATAT TGAAAGAATG AACTATATTC ACCGAGATCT TCGGGCTGCT 1200

AATATTCTTG TAGGAGAAAA TCTTGTGTGC AAAATAGCAG ACTTTGGTTT AGCAAGGTTA 1260

ATTGAAGACA ATGAATACAC AGCAAGACAA GGTGCAAAAT TTCCAATCAA ATGGACAGCT 1320

CCTGAAGCTG CACTGTATGG TCGGTTTACA ATAAAGTCTG ATGTCTGGTC ATTTGGAATT 1380

CTGCAAACAG AACTAGTAAC AAAGGGCCGA GTGCCATATC CAGGTATGGT GAACCGTGAA 1440

GTATTAGAAC AAGTGGAGCG AGGATACAGG ATGCCGTGCC CTCAGGGCTG TCCAGAATCC 1500

CTCCATGAAT TGATGAATCT GTGTTGGAAG AAGGACCCTG ATGAAAGACC AACATTTGAA 1560

TATATTCAGT CCTTCTTGGA AGACTACTTC ACTGCTACAG AGCCACAGTA CCAGCCAGGA 1620

GAAAATTTAT AA 1632

Domain Profile

S: 1     lklvkklGeGqfGevwlgkwkgsvkvavktlkegtlspeafleeaqilkklrheklvkly  60

         l+l+ klG+G+fGevw+g+w+g++kva+ktlk+gt++peafl+eaqi+kklrh+klv ly

Q: 277   LRLEVKLGQGCFGEVWMGTWNGTTKVAIKTLKPGTMMPEAFLQEAQIMKKLRHDKLVPLY  336

         68999*******************************************************

S: 61    avvseeePiyivtelmakGslldflkeeegkklklpklvdlaaqvaeGmayleeknlihr  120

         avvse ePiyivte+m+kGslldflke +gk+lklp+lvd+aaq+a+Gmay+e++n+ihr

Q: 337   AVVSE-EPIYIVTEFMSKGSLLDFLKEGDGKYLKLPQLVDMAAQIADGMAYIERMNYIHR  395

         ****9.******************************************************

S: 121   dlaarnvlvgeslvvkvadfGlarlieddeytakegaklPikWtaPeaiklgkftiksdv  180

         dl+a+n+lvge+lv+k+adfGlarlied+eyta++gak+PikWtaPea+ +g+ftiksdv

Q: 396   DLRAANILVGENLVCKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDV  455

         ************************************************************

S: 181   WsfGillteivtkGkvPypGmtneevleqvergyrlprpeecpeelyelllecwkkdpee  240

         WsfGil te+vtkG+vPypGm n+evleqvergyr+p+p+ cpe+l+el++ cwkkdp+e

Q: 456   WSFGILQTELVTKGRVPYPGMVNREVLEQVERGYRMPCPQGCPESLHELMNLCWKKDPDE  515

         ************************************************************

S: 241   rPtfetlqevl  251

         rPtfe++q++l

Q: 516   RPTFEYIQSFL  526

         *******9986

Domain Sequence
(FASTA)

LRLEVKLGQG CFGEVWMGTW NGTTKVAIKT LKPGTMMPEA FLQEAQIMKK LRHDKLVPLY 60

AVVSEEPIYI VTEFMSKGSL LDFLKEGDGK YLKLPQLVDM AAQIADGMAY IERMNYIHRD 120

LRAANILVGE NLVCKIADFG LARLIEDNEY TARQGAKFPI KWTAPEAALY GRFTIKSDVW 180

SFGILQTELV TKGRVPYPGM VNREVLEQVE RGYRMPCPQG CPESLHELMN LCWKKDPDER 240

PTFEYIQSFL 250

Keyword

3D-structure; ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Lipoprotein; Membrane; Myristate; Nucleotide-binding; Palmitate; Phosphoprotein; Polymorphism; Proto-oncogene; Reference proteome; SH2 domain; SH3 domain; Transferase; Tyrosine-protein kinase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005829	; C:cytosol
GO:0005794	; C:Golgi apparatus
GO:0005815	; C:microtubule organizing center
GO:0005886	; C:plasma membrane
GO:0005524	; F:ATP binding
GO:0004715	; F:non-membrane spanning protein tyrosine kinase activity
GO:0007596	; P:blood coagulation
GO:0050900	; P:leukocyte migration
GO:0043114	; P:regulation of vascular permeability
GO:0031295	; P:T cell costimulation

KEGG

hsa:7525

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR000980	; SH2.
IPR001452	; SH3_domain.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.

Pfam

PF07714	; Pkinase_Tyr; 1.
PF00017	; SH2; 1.
PF00018	; SH3_1; 1.

SMARTs

SM00252	; SH2; 1.
SM00326	; SH3; 1.
SM00219	; TyrKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS50001	; SH2; 1.
PS50002	; SH3; 1.

Prints

PR00401	; SH2DOMAIN.
PR00452	; SH3DOMAIN.
PR00109	; TYRKINASE.

Created Date

20-Feb-2013