EKS-HOS-00440

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00440

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/ULK	391.4	1.1E-116	16	278	263

Status

Reviewed

Ensembl Protein

ENSP00000324560

UniProt Accession

O75385; Q9UQ28;

Protein Name

Serine/threonine-protein kinase ULK1

Protein Synonyms/Alias

Autophagy-related protein 1 homolog; ATG1; hATG1; Unc-51-like kinase 1;

Gene Name

ULK1

Gene Synonyms/Alias

ULK1; KIAA0722;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000177169	ENSP00000444298	ENST00000541761
ENSG00000177169	ENSP00000439648	ENST00000538444
ENSG00000177169	ENSP00000441794	ENST00000540647
ENSG00000177169	ENSP00000446219	ENST00000542419
ENSG00000177169	ENSP00000444983	ENST00000542313
ENSG00000177169	ENSP00000324560	ENST00000321867
ENSG00000177169	ENSP00000438953	ENST00000537421

Organism

Homo sapiens

Functional Description

Serine/threonine-protein kinase involved in autophagy inresponse to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation.

Protein Length

1050

Protein Sequence
(FASTA)

MEPGRGGTET VGKFEFSRKD LIGHGAFAVV FKGRHREKHD LEVAVKCINK KNLAKSQTLL 60

GKEIKILKEL KHENIVALYD FQEMANSVYL VMEYCNGGDL ADYLHAMRTL SEDTIRLFLQ 120

QIAGAMRLLH SKGIIHRDLK PQNILLSNPA GRRANPNSIR VKIADFGFAR YLQSNMMAAT 180

LCGSPMYMAP EVIMSQHYDG KADLWSIGTI VYQCLTGKAP FQASSPQDLR LFYEKNKTLV 240

PTIPRETSAP LRQLLLALLQ RNHKDRMDFD EFFHHPFLDA SPSVRKSPPV PVPSYPSSGS 300

GSSSSSSSTS HLASPPSLGE MQQLQKTLAS PADTAGFLHS SRDSGGSKDS SCDTDDFVMV 360

PAQFPGDLVA EAPSAKPPPD SLMCSGSSLV ASAGLESHGR TPSPSPPCSS SPSPSGRAGP 420

FSSSRCGASV PIPVPTQVQN YQRIERNLQS PTQFQTPRSS AIRRSGSTSP LGFARASPSP 480

PAHAEHGGVL ARKMSLGGGR PYTPSPQVGT IPERPGWSGT PSPQGAEMRG GRSPRPGSSA 540

PEHSPRTSGL GCRLHSAPNL SDLHVVRPKL PKPPTDPLGA VFSPPQASPP QPSHGLQSCR 600

NLRGSPKLPD FLQRNPLPPI LGSPTKAVPS FDFPKTPSSQ NLLALLARQG VVMTPPRNRT 660

LPDLSEVGPF HGQPLGPGLR PGEDPKGPFG RSFSTSRLTD LLLKAAFGTQ APDPGSTESL 720

QEKPMEIAPS AGFGGSLHPG ARAGGTSSPS PVVFTVGSPP SGSTPPQGPR TRMFSAGPTG 780

SASSSARHLV PGPCSEAPAP ELPAPGHGCS FADPITANLE GAVTFEAPDL PEETLMEQEH 840

TEILRGLRFT LLFVQHVLEI AALKGSASEA AGGPEYQLQE SVVADQISLL SREWGFAEQL 900

VLYLKVAELL SSGLQSAIDQ IRAGKLCLSS TVKQVVRRLN ELYKASVVSC QGLSLRLQRF 960

FLDKQRLLDR IHSITAERLI FSHAVQMVQS AALDEMFQHR EGCVPRYHKA LLLLEGLQHM 1020

LSDQADIENV TKCKLCIERR LSALLTGICA 1050

Nucleotide Sequence
(FASTA)

ATGGAGCCCG GCCGCGGCGG CACAGAGACC GTGGGCAAGT TCGAGTTCTC CCGCAAGGAC 60

CTGATCGGCC ACGGCGCCTT CGCGGTGGTC TTCAAGGGCC GCCACCGCGA GAAGCACGAT 120

TTGGAGGTCG CCGTCAAGTG CATTAACAAG AAGAACCTCG CCAAGTCTCA GACGCTGCTG 180

GGGAAGGAAA TCAAAATCCT GAAGGAACTG AAACATGAAA ACATCGTGGC CCTGTACGAC 240

TTCCAGGAAA TGGCTAATTC TGTCTACCTG GTTATGGAGT ACTGCAACGG TGGGGACCTG 300

GCCGACTACC TGCACGCCAT GCGCACGCTG AGCGAGGACA CCATCAGGCT CTTCCTGCAG 360

CAGATCGCGG GCGCCATGCG GCTTCTGCAC AGCAAAGGCA TCATCCACCG CGACCTGAAA 420

CCGCAGAACA TCCTGCTGTC CAACCCCGCC GGCCGCCGCG CCAACCCCAA CAGCATCCGC 480

GTCAAGATCG CTGACTTCGG CTTCGCGCGG TACCTCCAGA GCAACATGAT GGCGGCCACA 540

CTCTGCGGCT CCCCCATGTA CATGGCCCCC GAGGTCATCA TGTCCCAGCA CTACGACGGG 600

AAGGCGGACC TGTGGAGCAT CGGCACCATC GTCTACCAGT GCCTGACGGG GAAGGCGCCC 660

TTCCAGGCCA GCAGCCCCCA GGACCTGCGC CTGTTCTACG AGAAGAACAA GACGTTGGTC 720

CCCACCATCC CCCGGGAGAC CTCGGCCCCG CTGCGGCAGC TGCTCCTGGC CCTACTGCAA 780

CGCAACCACA AGGACCGCAT GGACTTCGAT GAGTTTTTTC ATCACCCTTT CCTCGATGCC 840

AGCCCCTCGG TCAGGAAATC CCCACCCGTG CCTGTGCCCT CGTACCCAAG CTCGGGGTCC 900

GGCAGCAGCT CCAGCAGCAG CTCCACCTCC CACCTGGCCT CCCCGCCGTC CCTGGGCGAG 960

ATGCAGCAGC TGCAGAAGAC CCTGGCCTCC CCGGCTGACA CCGCTGGCTT CCTGCACAGC 1020

TCCCGGGACT CTGGTGGCAG CAAGGACTCT TCCTGTGACA CAGACGACTT CGTCATGGTC 1080

CCCGCGCAGT TTCCAGGTGA CCTGGTGGCT GAGGCGCCCA GTGCCAAACC CCCGCCAGAC 1140

AGCCTGATGT GCAGTGGGAG CTCACTGGTG GCCTCTGCGG GCTTGGAGAG CCACGGCCGG 1200

ACCCCATCTC CATCCCCACC CTGCAGCAGC TCCCCCAGTC CCTCAGGCCG GGCTGGCCCG 1260

TTCTCCAGCA GCAGGTGCGG CGCCTCTGTC CCCATCCCAG TCCCCACGCA GGTGCAGAAC 1320

TACCAGCGCA TTGAGCGAAA CCTGCAGTCA CCCACCCAGT TCCAAACACC TCGGTCCTCT 1380

GCCATCCGCA GGTCAGGCAG CACCAGCCCC CTGGGCTTTG CAAGGGCCAG CCCCTCGCCC 1440

CCTGCCCACG CTGAGCATGG AGGCGTCCTG GCCAGGAAGA TGTCTCTGGG TGGAGGCCGG 1500

CCCTACACGC CATCTCCTCA AGTTGGAACC ATCCCTGAGC GGCCAGGCTG GAGCGGGACG 1560

CCCTCCCCAC AGGGAGCTGA GATGCGGGGT GGCAGGTCCC CTCGTCCAGG CTCCTCTGCA 1620

CCCGAGCACT CTCCCCGCAC TTCCGGGCTG GGCTGCCGCC TGCACAGCGC CCCCAACCTG 1680

TCTGACTTGC ACGTCGTCCG CCCCAAGCTG CCCAAACCCC CCACGGACCC CCTGGGAGCT 1740

GTGTTCAGCC CACCACAGGC CAGCCCTCCC CAGCCGTCCC ACGGCCTGCA GTCCTGCCGG 1800

AACCTGCGGG GCTCACCCAA GCTGCCCGAC TTCCTGCAGC GAAACCCCCT GCCCCCCATC 1860

CTGGGCTCCC CCACCAAGGC TGTGCCCTCC TTTGACTTCC CGAAGACCCC CAGCTCCCAG 1920

AACCTGCTGG CCCTCCTAGC CCGGCAGGGC GTGGTGATGA CGCCCCCTCG AAACCGGACG 1980

CTGCCCGACC TCTCGGAGGT GGGACCCTTC CATGGTCAGC CGTTGGGCCC TGGCCTGCGG 2040

CCAGGCGAGG ACCCCAAGGG CCCCTTTGGC CGGTCTTTCA GCACCAGCCG CCTCACTGAC 2100

CTGCTCCTTA AGGCGGCGTT TGGGACACAA GCCCCGGACC CGGGCAGCAC GGAGAGCCTG 2160

CAGGAGAAGC CCATGGAGAT CGCACCCTCA GCTGGCTTTG GAGGGAGCCT GCACCCAGGA 2220

GCCCGTGCTG GGGGCACCAG CAGCCCTTCC CCGGTGGTCT TCACCGTGGG CTCTCCCCCG 2280

AGCGGGAGCA CGCCCCCCCA GGGCCCCCGC ACCAGGATGT TCTCAGCGGG CCCCACTGGC 2340

TCTGCCAGCT CTTCTGCCCG CCACCTGGTG CCTGGGCCCT GCAGCGAGGC CCCAGCCCCT 2400

GAGCTCCCTG CTCCAGGACA CGGCTGCAGC TTTGCCGACC CCATTACTGC GAACCTGGAG 2460

GGGGCTGTGA CCTTCGAGGC CCCCGACCTC CCTGAGGAGA CCCTCATGGA GCAAGAGCAC 2520

ACGGAGATCC TGCGTGGCCT GCGCTTCACG CTGCTGTTCG TGCAGCACGT CCTGGAGATC 2580

GCAGCCCTGA AGGGCAGCGC CAGTGAGGCG GCGGGGGGCC CTGAGTACCA GCTGCAGGAG 2640

AGTGTGGTGG CCGACCAGAT CAGCCTGCTG AGCCGAGAAT GGGGCTTCGC GGAACAGCTG 2700

GTGCTGTACC TGAAGGTGGC CGAGCTACTG TCCTCCGGCC TGCAAAGTGC CATCGACCAG 2760

ATCCGGGCCG GCAAGCTCTG CCTGTCGTCC ACTGTGAAGC AGGTGGTGCG CAGGCTGAAT 2820

GAGCTGTACA AGGCCAGCGT GGTGTCCTGC CAGGGCCTGA GCCTGCGGCT GCAGCGCTTC 2880

TTCCTGGACA AGCAGCGGCT CCTGGACCGC ATTCACAGCA TCACTGCCGA GAGGCTCATC 2940

TTCAGCCACG CTGTGCAGAT GGTGCAGTCG GCTGCCCTGG ACGAGATGTT CCAGCACCGT 3000

GAGGGCTGCG TCCCACGCTA CCACAAGGCC CTGCTGCTCC TGGAGGGGCT GCAGCACATG 3060

CTCTCGGACC AGGCCGACAT CGAGAACGTC ACCAAGTGCA AGCTGTGCAT TGAGCGGAGA 3120

CTCTCGGCGC TGCTGACTGG CATCTGTGCC TGA 3153

Domain Profile

S: 1     ysleeeiGkGsfavvykgrlrkktsqvvAikvvekkklkkslenlekeikilkelkheni  60

         +s ++ iG+G+favv+kgr+r+k++ +vA+k+++kk+l+ks++ l keikilkelkheni

Q: 16    FSRKDLIGHGAFAVVFKGRHREKHDLEVAVKCINKKNLAKSQTLLGKEIKILKELKHENI  75

         7889********************************************************

S: 61    valldveetskevylvmeycaggdLakllkqkkeLsedtvreflqqlasalkaLhskkiv  120

         val+d++e++++vylvmeyc+ggdLa++l+++++Lsedt+r flqq+a a+++Lhsk+i+

Q: 76    VALYDFQEMANSVYLVMEYCNGGDLADYLHAMRTLSEDTIRLFLQQIAGAMRLLHSKGII  135

         ************************************************************

S: 121   hrDlkPqniLlsyadakk.elkkvtlkladFglarvlkeesmaatlkGsplYmaPEvvke  179

         hrDlkPqniLls+  +++ + +++++k+adFg+ar+l++++maatl+Gsp+YmaPEv+++

Q: 136   HRDLKPQNILLSNPAGRRaNPNSIRVKIADFGFARYLQSNMMAATLCGSPMYMAPEVIMS  195

         ************99999999****************************************

S: 180   qqydakaDlWSiGtilyellvGkpPfaaesiseLvekiekndvleppipkeisselkdll  239

         q+yd kaDlWSiGti+y++l+Gk+Pf+a+s+++L+ ++ekn++l p+ip+e+s+ l++ll

Q: 196   QHYDGKADLWSIGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTLVPTIPRETSAPLRQLL  255

         ************************************************************

S: 240   qsLLqrdpkkRisfeelfahpfl  262

          +LLqr++k+R++f+e+f+hpfl

Q: 256   LALLQRNHKDRMDFDEFFHHPFL  278

         *********************96

Domain Sequence
(FASTA)

FSRKDLIGHG AFAVVFKGRH REKHDLEVAV KCINKKNLAK SQTLLGKEIK ILKELKHENI 60

VALYDFQEMA NSVYLVMEYC NGGDLADYLH AMRTLSEDTI RLFLQQIAGA MRLLHSKGII 120

HRDLKPQNIL LSNPAGRRAN PNSIRVKIAD FGFARYLQSN MMAATLCGSP MYMAPEVIMS 180

QHYDGKADLW SIGTIVYQCL TGKAPFQASS PQDLRLFYEK NKTLVPTIPR ETSAPLRQLL 240

LALLQRNHKD RMDFDEFFHH PFL 263

Keyword

ATP-binding; Autophagy; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005776	; C:autophagic vacuole
GO:0030659	; C:cytoplasmic vesicle membrane
GO:0005829	; C:cytosol
GO:0043005	; C:neuron projection
GO:0043025	; C:neuronal cell body
GO:0034045	; C:pre-autophagosomal structure membrane
GO:0070969	; C:ULK1-ATG13-FIP200 complex
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0000045	; P:autophagic vacuole assembly
GO:0048675	; P:axon extension
GO:0031669	; P:cellular response to nutrient levels
GO:0021707	; P:cerebellar granule cell differentiation
GO:0048671	; P:negative regulation of collateral sprouting
GO:0031175	; P:neuron projection development
GO:0031102	; P:neuron projection regeneration
GO:0016239	; P:positive regulation of macroautophagy
GO:0046777	; P:protein autophosphorylation
GO:0008104	; P:protein localization
GO:0021933	; P:radial glia guided migration of cerebellar granule cell
GO:0007265	; P:Ras protein signal transduction
GO:0031623	; P:receptor internalization
GO:0051386	; P:regulation of nerve growth factor receptor signaling pathway
GO:0042594	; P:response to starvation

KEGG

hsa:8408

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR016237	; Ser/Thr_kin_STPK_Ulk-1/2.
IPR008271	; Ser/Thr_kinase_AS.
IPR022708	; Ser/Thr_kinase_C.

Pfam

PF12063	; DUF3543; 1.
PF00069	; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013