EKS-HOS-00121

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00121

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/NAK	362.6	6.2E-108	41	313	273

Status

Reviewed

Ensembl Protein

ENSP00000314499

UniProt Accession

O14976; Q9BVY6;

Protein Name

Cyclin-G-associated kinase

Protein Synonyms/Alias

Gene Name

GAK

Gene Synonyms/Alias

GAK;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000178950	ENSP00000421814	ENST00000512325
ENSG00000178950	ENSP00000424553	ENST00000504668
ENSG00000178950	ENSP00000314499	ENST00000314167
ENSG00000178950	ENSP00000426062	ENST00000510799
ENSG00000178950	ENSP00000426728	ENST00000507580
ENSG00000178950	ENSP00000426908	ENST00000505819
ENSG00000178950	ENSP00000421361	ENST00000511163
ENSG00000178950	ENSP00000424701	ENST00000502656
ENSG00000178950	ENSP00000422391	ENST00000511229
ENSG00000178950	ENSP00000421049	ENST00000511980

Organism

Homo sapiens

Functional Description

Associates with cyclin G and CDK5. Seems to act as anauxilin homolog that is involved in the uncoating of clathrin- coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.

Protein Length

1311

Protein Sequence
(FASTA)

MSLLQSALDF LAGPGSLGGA SGRDQSDFVG QTVELGELRL RVRRVLAEGG FAFVYEAQDV 60

GSGREYALKR LLSNEEEKNR AIIQEVCFMK KLSGHPNIVQ FCSAASIGKE ESDTGQAEFL 120

LLTELCKGQL VEFLKKMESR GPLSCDTVLK IFYQTCRAVQ HMHRQKPPII HRDLKVENLL 180

LSNQGTIKLC DFGSATTISH YPDYSWSAQR RALVEEEITR NTTPMYRTPE IIDLYSNFPI 240

GEKQDIWALG CILYLLCFRQ HPFEDGAKLR IVNGKYSIPP HDTQYTVFHS LIRAMLQVNP 300

EERLSIAEVV HQLQEIAAAR NVNPKSPITE LLEQNGGYGS ATLSRGPPPP VGPAGSGYSG 360

GLALAEYDQP YGGFLDILRG GTERLFTNLK DTSSKVIQSV ANYAKGDLDI SYITSRIAVM 420

SFPAEGVESA LKNNIEDVRL FLDSKHPGHY AVYNLSPRTY RPSRFHNRVS ECGWAARRAP 480

HLHTLYNICR NMHAWLRQDH KNVCVVHCMD GRAASAVAVC SFLCFCRLFS TAEAAVYMFS 540

MKRCPPGIWP SHKRYIEYMC DMVAEEPITP HSKPILVRAV VMTPVPLFSK QRSGCRPFCE 600

VYVGDERVAS TSQEYDKMRD FKIEDGKAVI PLGVTVQGDV LIVIYHARST LGGRLQAKMA 660

SMKMFQIQFH TGFVPRNATT VKFAKYDLDA CDIQEKYPDL FQVNLEVEVE PRDRPSREAP 720

PWENSSMRGL NPKILFSSRE EQQDILSKFG KPELPRQPGS TAQYDAGAGS PEAEPTDSDS 780

PPSSSADASR FLHTLDWQEE KEAETGAENA SSKESESALM EDRDESEVSD EGGSPISSEG 840

QEPRADPEPP GLAAGLVQQD LVFEVETPAV LPEPVPQEDG VDLLGLHSEV GAGPAVPPQA 900

CKAPSSNTDL LSCLLGPPEA ASQGPPEDLL SEDPLLLASP APPLSVQSTP RGGPPAAADP 960

FGPLLPSSGN NSQPCSNPDL FGEFLNSDSV TVPPSFPSAH SAPPPSCSAD FLHLGDLPGE 1020

PSKMTASSSN PDLLGGWAAW TETAASAVAP TPATEGPLFS PGGQPAPCGS QASWTKSQNP 1080

DPFADLGDLS SGLQGSPAGF PPGGFIPKTA TTPKGSSSWQ TSRPPAQGAS WPPQAKPPPK 1140

ACTQPRPNYA SNFSVIGARE ERGVRAPSFA QKPKVSENDF EDLLSNQGFS SRSDKKGPKT 1200

IAEMRKQDLA KDTDPLKLKL LDWIEGKERN IRALLSTLHT VLWDGESRWT PVGMADLVAP 1260

EQVKKHYRRA VLAVHPDKAA GQPYEQHAKM IFMELNDAWS EFENQGSRPL F 1311

Nucleotide Sequence
(FASTA)

ATGTCGCTGC TGCAGTCGGC GCTCGACTTC TTGGCGGGTC CAGGCTCCCT GGGCGGTGCT 60

TCCGGCCGCG ACCAGAGTGA CTTCGTGGGG CAGACGGTGG AACTGGGCGA GCTGCGGCTG 120

CGGGTGCGGC GGGTCCTGGC CGAAGGAGGG TTTGCATTTG TGTATGAAGC TCAAGATGTG 180

GGGAGTGGCA GAGAGTATGC ATTAAAGAGG CTATTATCCA ATGAAGAGGA AAAGAACAGA 240

GCCATCATTC AAGAAGTTTG CTTCATGAAA AAGCTTTCCG GCCACCCGAA CATTGTCCAG 300

TTTTGTTCTG CAGCGTCTAT AGGAAAAGAG GAGTCAGACA CGGGGCAGGC TGAGTTCCTC 360

TTGCTCACAG AGCTCTGTAA AGGGCAGCTG GTGGAATTTT TGAAGAAAAT GGAATCTCGA 420

GGCCCCCTTT CGTGCGACAC GGTTCTGAAG ATCTTCTACC AGACGTGCCG CGCCGTGCAG 480

CACATGCACC GGCAGAAGCC GCCCATCATC CACAGGGACC TCAAGGTTGA GAACTTGTTG 540

CTTAGTAACC AAGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACGAC CATCTCGCAC 600

TACCCTGACT ACAGCTGGAG CGCCCAGAGG CGAGCCCTGG TGGAGGAAGA GATCACGAGG 660

AATACAACAC CAATGTATAG AACACCAGAA ATCATAGACT TGTATTCCAA CTTCCCGATC 720

GGCGAGAAGC AGGATATCTG GGCCCTGGGC TGCATCTTGT ACCTGCTGTG CTTCCGGCAG 780

CACCCTTTTG AGGATGGAGC GAAACTTCGA ATAGTCAATG GGAAGTACTC GATCCCCCCG 840

CACGACACGC AGTACACGGT CTTCCACAGC CTCATCCGCG CCATGCTGCA GGTGAACCCG 900

GAGGAGCGGC TGTCCATCGC CGAGGTGGTG CACCAGCTGC AGGAGATCGC GGCCGCCCGC 960

AACGTGAACC CCAAGTCTCC CATCACAGAG CTCCTGGAGC AGAATGGAGG CTACGGGAGC 1020

GCCACACTGT CCCGAGGGCC ACCCCCTCCC GTGGGCCCCG CTGGCAGTGG CTACAGTGGA 1080

GGCCTGGCGC TGGCGGAGTA CGACCAGCCG TATGGCGGCT TCCTGGACAT TCTGCGGGGT 1140

GGGACAGAGC GGCTCTTCAC CAACCTCAAG GACACCTCCT CCAAGGTCAT CCAGTCCGTC 1200

GCTAATTATG CAAAGGGTGA CCTGGACATA TCTTACATCA CATCCAGAAT TGCAGTGATG 1260

TCATTCCCAG CAGAAGGTGT GGAGTCAGCG CTCAAAAACA ACATCGAAGA TGTGCGGTTG 1320

TTCCTGGACT CCAAGCACCC AGGGCACTAT GCCGTCTACA ACCTGTCCCC GAGGACCTAC 1380

CGGCCCTCCA GGTTCCACAA CCGGGTCTCC GAGTGTGGCT GGGCAGCACG GCGGGCCCCA 1440

CACCTGCACA CCCTGTACAA CATCTGCAGG AACATGCACG CCTGGCTGCG GCAGGACCAC 1500

AAGAACGTCT GCGTCGTGCA CTGCATGGAC GGGAGAGCCG CGTCTGCTGT GGCCGTCTGC 1560

TCCTTCCTGT GCTTCTGCCG TCTCTTCAGC ACCGCGGAGG CCGCCGTGTA CATGTTCAGC 1620

ATGAAGCGCT GCCCACCAGG CATCTGGCCA TCCCACAAAA GGTACATCGA GTACATGTGT 1680

GACATGGTGG CGGAGGAGCC CATCACACCC CACAGCAAGC CCATCCTGGT GAGGGCCGTG 1740

GTCATGACAC CCGTGCCGCT GTTCAGCAAG CAGAGGAGCG GCTGCAGGCC CTTCTGCGAG 1800

GTCTACGTGG GGGACGAGCG TGTGGCCAGC ACCTCCCAGG AGTACGACAA GATGCGGGAC 1860

TTTAAGATTG AAGATGGCAA AGCGGTGATT CCCCTGGGCG TCACGGTGCA AGGAGACGTG 1920

CTCATCGTCA TCTATCACGC CCGGTCCACT CTGGGCGGCC GGCTGCAGGC CAAGATGGCA 1980

TCCATGAAGA TGTTCCAGAT TCAGTTCCAC ACGGGGTTTG TGCCTCGGAA CGCCACCACT 2040

GTGAAATTTG CCAAGTATGA CCTGGACGCG TGTGACATTC AAGAAAAATA CCCGGATTTA 2100

TTTCAAGTGA ACCTGGAAGT GGAGGTGGAG CCCAGGGACA GGCCGAGCCG GGAAGCCCCA 2160

CCATGGGAGA ACTCGAGCAT GAGGGGGCTG AACCCCAAAA TCCTGTTTTC CAGCCGGGAG 2220

GAGCAGCAAG ACATTCTGTC TAAGTTTGGG AAGCCGGAGC TTCCCCGGCA GCCTGGCTCC 2280

ACGGCTCAGT ATGATGCTGG GGCAGGGTCC CCGGAAGCCG AACCCACAGA CTCTGACTCA 2340

CCGCCAAGCA GCAGCGCGGA CGCCAGTCGC TTCCTGCACA CGCTGGACTG GCAGGAAGAG 2400

AAGGAGGCAG AGACTGGTGC AGAAAATGCC TCTTCCAAGG AGAGCGAGTC TGCCCTGATG 2460

GAGGACAGAG ACGAGAGTGA GGTGTCAGAT GAAGGGGGAT CCCCGATCTC CAGCGAGGGC 2520

CAGGAACCCA GGGCCGACCC AGAGCCCCCC GGCCTGGCAG CAGGGCTGGT GCAGCAGGAC 2580

TTGGTTTTTG AGGTGGAGAC ACCGGCTGTG CTGCCAGAGC CTGTGCCACA GGAAGACGGG 2640

GTCGACCTCC TGGGCCTGCA CTCCGAGGTG GGCGCAGGGC CAGCTGTACC CCCGCAGGCC 2700

TGCAAGGCCC CCTCCAGCAA CACCGACCTG CTCAGCTGCC TCCTTGGGCC CCCTGAGGCC 2760

GCCTCCCAGG GGCCCCCGGA GGATCTGCTC AGCGAGGACC CGCTGCTCCT GGCAAGCCCG 2820

GCCCCTCCCC TGAGCGTGCA GAGCACCCCA AGAGGAGGGC CCCCTGCCGC TGCTGACCCC 2880

TTTGGCCCGC TTCTGCCGTC TTCAGGCAAC AACTCCCAGC CCTGCTCCAA TCCTGATCTC 2940

TTCGGCGAAT TTCTCAATTC GGACTCTGTG ACCGTCCCAC CATCCTTCCC GTCTGCCCAC 3000

AGTGCTCCGC CCCCATCCTG CAGCGCCGAC TTCCTGCACC TGGGGGATCT GCCAGGAGAG 3060

CCCAGCAAGA TGACAGCCTC GTCCAGCAAC CCAGACCTGC TGGGAGGATG GGCTGCCTGG 3120

ACCGAGACTG CAGCATCGGC AGTGGCCCCC ACGCCAGCCA CAGAAGGCCC CCTCTTCTCT 3180

CCTGGAGGTC AGCCGGCCCC TTGTGGCTCT CAGGCCAGCT GGACCAAGTC TCAGAACCCG 3240

GACCCATTTG CTGACCTTGG CGACCTCAGC TCCGGCCTCC AAGGCTCACC AGCTGGATTC 3300

CCTCCTGGGG GCTTCATTCC CAAAACGGCC ACCACGCCCA AAGGCAGCAG CTCCTGGCAG 3360

ACAAGTCGGC CGCCAGCCCA GGGCGCCTCA TGGCCCCCTC AGGCCAAGCC GCCCCCCAAA 3420

GCCTGCACAC AGCCAAGGCC TAACTATGCC TCGAACTTCA GTGTGATCGG GGCGCGGGAG 3480

GAGCGGGGGG TCCGCGCACC CAGCTTTGCT CAAAAGCCAA AAGTCTCTGA GAACGACTTT 3540

GAAGATCTGT TGTCCAATCA AGGCTTCTCC TCCAGGTCTG ACAAGAAAGG GCCAAAGACC 3600

ATTGCAGAGA TGAGGAAGCA GGACCTGGCT AAAGACACGG ACCCACTCAA GCTGAAGCTC 3660

CTGGACTGGA TTGAGGGCAA GGAGCGGAAC ATCCGGGCCC TGCTGTCCAC GCTGCACACA 3720

GTGCTGTGGG ACGGGGAGAG CCGCTGGACG CCCGTGGGCA TGGCCGACCT GGTGGCTCCG 3780

GAGCAAGTGA AGAAGCACTA TCGCCGCGCG GTGCTGGCTG TGCACCCCGA CAAGGCTGCG 3840

GGGCAGCCGT ACGAGCAGCA CGCCAAGATG ATCTTCATGG AGCTGAATGA CGCCTGGTCG 3900

GAGTTTGAGA ACCAGGGCTC CCGGCCCCTC TTCTGA 3936

Domain Profile

S: 2     riekvlaeGGfalvylaedastgekyalKrilvneeedleeikrEieimkklsghknivk  61

         r+++vlaeGGfa+vy a+d+ +g++yalKr+l+neee++++i++E+++mkklsgh+niv+

Q: 41    RVRRVLAEGGFAFVYEAQDVGSGREYALKRLLSNEEEKNRAIIQEVCFMKKLSGHPNIVQ  100

         89**********************************************************

S: 62    lv..dssvkeekdngskevlllleyckkgslvdllnkrlekgelseaeilkilldvtrav  119

         ++  +s  kee+d+g++e+lll+e+ck g+lv++l+k++++g ls++++lki+++++rav

Q: 101   FCsaASIGKEESDTGQAEFLLLTELCK-GQLVEFLKKMESRGPLSCDTVLKIFYQTCRAV  159

         **877888999****************.8*******************************

S: 120   aalHalsvpliHRDlkveNillsdegelvlcDlGSattaileve...skqelsalqeeie  176

         +++H++++p+iHRDlkveN+lls++g+++lcD+GSatt+++ ++   s+q+++ ++eei+

Q: 160   QHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDyswSAQRRALVEEEIT  219

         *******************************************999999***********

S: 177   knttlsyrsPElidlysnlvideksDiWaLGcllYklcffesPfeesvklailngkysiP  236

         +ntt++yr+PE+idlysn++i+ek+DiWaLGc+lY lcf+++Pfe+++kl+i+ngkysiP

Q: 220   RNTTPMYRTPEIIDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIP  279

         ************************************************************

S: 237   ensryseklkslikkmlevdpeeRptiaqvlell  270

         +++++++ ++sli++ml+v+peeR++ia+v+++l

Q: 280   PHDTQYTVFHSLIRAMLQVNPEERLSIAEVVHQL  313

         ******************************9986

Domain Sequence
(FASTA)

RVRRVLAEGG FAFVYEAQDV GSGREYALKR LLSNEEEKNR AIIQEVCFMK KLSGHPNIVQ 60

FCSAASIGKE ESDTGQAEFL LLTELCKGQL VEFLKKMESR GPLSCDTVLK IFYQTCRAVQ 120

HMHRQKPPII HRDLKVENLL LSNQGTIKLC DFGSATTISH YPDYSWSAQR RALVEEEITR 180

NTTPMYRTPE IIDLYSNFPI GEKQDIWALG CILYLLCFRQ HPFEDGAKLR IVNGKYSIPP 240

HDTQYTVFHS LIRAMLQVNP EERLSIAEVV HQL 273

Keyword

3D-structure; ATP-binding; Cell cycle; Cell junction; Complete proteome; Cytoplasm; Golgi apparatus; Kinase; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005925	; C:focal adhesion
GO:0005794	; C:Golgi apparatus
GO:0048471	; C:perinuclear region of cytoplasm
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0007049	; P:cell cycle

KEGG

hsa:2580

;

InterPros

IPR008973	; C2_Ca/lipid-bd_dom_CaLB.
IPR001623	; DnaJ_N.
IPR011009	; Kinase-like_dom.
IPR014019	; Phosphatase_tensin-typ.
IPR000719	; Prot_kinase_cat_dom.
IPR008271	; Ser/Thr_kinase_AS.
IPR014020	; Tensin_phosphatase_C2-dom.

Pfam

PF00226	; DnaJ; 1.
PF00069	; Pkinase; 1.
PF10409	; PTEN_C2; 1.

SMARTs

SM00271

; DnaJ; 1.

Prosites

PS51182	; C2_TENSIN; 1.
PS00636	; DNAJ_1; FALSE_NEG.
PS50076	; DNAJ_2; 1.
PS51181	; PPASE_TENSIN; 1.
PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013