EKS-HOS-00324

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00324

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
STE/STE20	348.6	1.1E-103	249	500	252

Status

Reviewed

Ensembl Protein

ENSP00000314067

UniProt Accession

Q13177; Q13154; Q6ISC3;

Protein Name

PAK-2p34

Protein Synonyms/Alias

Gamma-PAK; PAK65; S6/H4 kinase; p21-activated kinase 2; PAK-2; p58; p27; p34; C-t-PAK2;

Gene Name

PAK2

Gene Synonyms/Alias

PAK2;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000180370	ENSP00000402927	ENST00000426668
ENSG00000180370	ENSP00000314067	ENST00000327134

Organism

Homo sapiens

Functional Description

Serine/threonine protein kinase that plays a role in avariety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF- induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase- activated PAK2 phosphorylates MKNK1 and reduces cellular translation.

Protein Length

524

Protein Sequence
(FASTA)

MSDNGELEDK PPAPPVRMSS TIFSTGGKDP LSANHSLKPL PSVPEEKKPR HKIISIFSGT 60

EKGSKKKEKE RPEISPPSDF EHTIHVGFDA VTGEFTGMPE QWARLLQTSN ITKLEQKKNP 120

QAVLDVLKFY DSNTVKQKYL SFTPPEKDGF PSGTPALNAK GTEAPAVVTE EEDDDEETAP 180

PVIAPRPDHT KSIYTRSVID PVPAPVGDSH VDGAAKSLDK QKKKTKMTDE EIMEKLRTIV 240

SIGDPKKKYT RYEKIGQGAS GTVFTATDVA LGQEVAIKQI NLQKQPKKEL IINEILVMKE 300

LKNPNIVNFL DSYLVGDELF VVMEYLAGGS LTDVVTETCM DEAQIAAVCR ECLQALEFLH 360

ANQVIHRDIK SDNVLLGMEG SVKLTDFGFC AQITPEQSKR STMVGTPYWM APEVVTRKAY 420

GPKVDIWSLG IMAIEMVEGE PPYLNENPLR ALYLIATNGT PELQNPEKLS PIFRDFLNRC 480

LEMDVEKRGS AKELLQHPFL KLAKPLSSLT PLIMAAKEAM KSNR 524

Nucleotide Sequence
(FASTA)

ATGTCTGATA ACGGAGAACT GGAAGATAAG CCTCCAGCAC CTCCTGTGCG AATGAGCAGC 60

ACCATCTTTA GCACTGGAGG CAAAGACCCT TTGTCAGCCA ATCACAGTTT GAAACCTTTG 120

CCCTCTGTTC CAGAAGAGAA AAAGCCCAGG CATAAAATCA TCTCCATATT CTCAGGCACA 180

GAGAAAGGAA GTAAAAAGAA AGAAAAGGAA CGGCCAGAAA TTTCTCCTCC ATCTGATTTT 240

GAGCACACCA TCCATGTTGG CTTTGATGCT GTTACTGGAG AATTCACTGG CATGCCAGAA 300

CAGTGGGCTC GATTACTACA GACCTCCAAT ATCACCAAAC TAGAGCAAAA GAAGAATCCT 360

CAGGCTGTGC TGGATGTCCT AAAGTTCTAC GACTCCAACA CAGTGAAGCA GAAATATCTG 420

AGCTTTACTC CTCCTGAGAA AGATGGCTTT CCTTCTGGAA CACCAGCACT GAATGCCAAG 480

GGAACAGAAG CACCCGCAGT AGTGACAGAG GAGGAGGATG ATGATGAAGA GACTGCTCCT 540

CCCGTTATTG CCCCGCGACC GGATCATACG AAATCAATTT ACACACGGTC TGTAATTGAC 600

CCTGTTCCTG CACCAGTTGG TGATTCACAT GTTGATGGTG CTGCCAAGTC TTTAGACAAA 660

CAGAAAAAGA AGACTAAGAT GACAGATGAA GAGATTATGG AGAAATTAAG AACTATCGTG 720

AGCATAGGTG ACCCTAAGAA AAAATATACA AGATATGAAA AAATTGGACA AGGGGCTTCT 780

GGTACAGTTT TCACTGCTAC TGACGTTGCA CTGGGACAGG AGGTTGCTAT CAAACAAATT 840

AATTTACAGA AACAGCCAAA GAAGGAACTG ATCATTAACG AGATTCTGGT GATGAAAGAA 900

TTGAAAAATC CCAACATCGT TAACTTTTTG GACAGTTACC TGGTAGGAGA TGAATTGTTT 960

GTGGTCATGG AATACCTTGC TGGGGGGTCA CTCACTGATG TGGTAACAGA AACGTGCATG 1020

GATGAAGCAC AGATTGCTGC TGTATGCAGA GAGTGTTTAC AGGCATTGGA GTTTTTACAT 1080

GCTAATCAAG TGATCCACAG AGACATCAAA AGTGACAATG TACTTTTGGG AATGGAAGGA 1140

TCTGTTAAGC TCACTGACTT TGGTTTCTGT GCCCAGATCA CCCCTGAGCA GAGCAAACGC 1200

AGTACCATGG TCGGAACGCC ATACTGGATG GCACCAGAGG TGGTTACACG GAAAGCTTAT 1260

GGCCCTAAAG TCGACATATG GTCTCTGGGT ATCATGGCTA TTGAGATGGT AGAAGGAGAG 1320

CCTCCATACC TCAATGAAAA TCCCTTGAGG GCCTTGTACC TAATAGCAAC TAATGGAACC 1380

CCAGAACTTC AGAATCCAGA GAAACTTTCC CCAATATTTC GGGATTTCTT AAATCGATGT 1440

TTGGAAATGG ATGTGGAAAA AAGGGGTTCA GCCAAAGAAT TATTACAGCA TCCTTTCCTG 1500

AAACTGGCCA AACCGTTATC TAGCTTGACA CCACTGATCA TGGCAGCTAA AGAAGCAATG 1560

AAGAGTAACC GTTAA 1575

Domain Profile

S: 1     yelleeigkGalgkvylarekktgelvaikkinlekqekleelqkeisvlkklkhpnive  60

         y+ +e+ig+Ga+g+v++a+++  g+ vaik+inl+kq+k+e++++ei v+k+lk+pniv+

Q: 249   YTRYEKIGQGASGTVFTATDVALGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVN  308

         7889********************************************************

S: 61    ylesflkeeelwlvmeylagGslsdlvkeelkselkEeqiayilrevlkaLeylHskkvi  120

         +l+s+l+ +el++vmeylagGsl+d+v+   ++ ++E qia+++re+l+aLe+lH+++vi

Q: 309   FLDSYLVGDELFVVMEYLAGGSLTDVVT---ETCMDEAQIAAVCRECLQALEFLHANQVI  365

         ****************************...9****************************

S: 121   HrdikakniLlteegeVkLsdfgvaaqlektrkkrktvvgtpywmAPEviaqeekgYdek  180

         Hrdik++n+Ll +eg+VkL+dfg++aq++ +++kr+t+vgtpywmAPEv++  +k Y  k

Q: 366   HRDIKSDNVLLGMEGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVT--RKAYGPK  423

         ***************************************************..9******

S: 181   aDiwslGItaielaegepPfkdleplkallkilkneppklkskkkfskefkefvekclqk  240

         +DiwslGI+aie++egepP+ +++pl+al++i++n +p+l++++k+s+ f++f+++cl+

Q: 424   VDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEM  483

         ************************************************************

S: 241   npekRpsasellkhkFl  257

         ++ekR sa+ell+h+Fl

Q: 484   DVEKRGSAKELLQHPFL  500

         ****************7

Domain Sequence
(FASTA)

YTRYEKIGQG ASGTVFTATD VALGQEVAIK QINLQKQPKK ELIINEILVM KELKNPNIVN 60

FLDSYLVGDE LFVVMEYLAG GSLTDVVTET CMDEAQIAAV CRECLQALEF LHANQVIHRD 120

IKSDNVLLGM EGSVKLTDFG FCAQITPEQS KRSTMVGTPY WMAPEVVTRK AYGPKVDIWS 180

LGIMAIEMVE GEPPYLNENP LRALYLIATN GTPELQNPEK LSPIFRDFLN RCLEMDVEKR 240

GSAKELLQHP FL 252

Keyword

3D-structure; Acetylation; Allosteric enzyme; Apoptosis; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Growth regulation; Host-virus interaction; Kinase; Lipoprotein; Membrane; Myristate; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sorex araneus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005829	; C:cytosol
GO:0005634	; C:nucleus
GO:0048471	; C:perinuclear region of cytoplasm
GO:0005886	; C:plasma membrane
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0030296	; F:protein tyrosine kinase activator activity
GO:0007411	; P:axon guidance
GO:0006921	; P:cellular component disassembly involved in execution phase of apoptosis
GO:0043066	; P:negative regulation of apoptotic process
GO:2001271	; P:negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis
GO:0006469	; P:negative regulation of protein kinase activity
GO:0018105	; P:peptidyl-serine phosphorylation
GO:2001238	; P:positive regulation of extrinsic apoptotic signaling pathway
GO:0046777	; P:protein autophosphorylation
GO:0050690	; P:regulation of defense response to virus by virus
GO:0040008	; P:regulation of growth
GO:0031295	; P:T cell costimulation
GO:0050852	; P:T cell receptor signaling pathway
GO:0016032	; P:viral reproduction
GO:0019048	; P:virus-host interaction

KEGG

hsa:5062

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000095	; PAK_box_Rho-bd.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00786	; PBD; 1.
PF00069	; Pkinase; 1.

SMARTs

SM00285	; PBD; 1.
SM00220	; S_TKc; 1.

Prosites

PS50108	; CRIB; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013