EKS-HOS-00008

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00008

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/Eph	509.1	8.6E-153	633	892	260

Status

Reviewed

Ensembl Protein

ENSP00000332118

UniProt Accession

P54753; Q7Z740;

Protein Name

Ephrin type-B receptor 3

Protein Synonyms/Alias

EPH-like tyrosine kinase 2; EPH-like kinase 2; Embryonic kinase 2; EK2; hEK2; Tyrosine-protein kinase TYRO6;

Gene Name

EPHB3

Gene Synonyms/Alias

EPHB3; ETK2, HEK2, TYRO6;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000182580	ENSP00000332118	ENST00000330394

Organism

Homo sapiens

Functional Description

Receptor tyrosine kinase which binds promiscuouslytransmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobs of the cerebral cortex. Beside its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.

Protein Length

998

Protein Sequence
(FASTA)

MARARPPPPP SPPPGLLPLL PPLLLLPLLL LPAGCRALEE TLMDTKWVTS ELAWTSHPES 60

GWEEVSGYDE AMNPIRTYQV CNVRESSQNN WLRTGFIWRR DVQRVYVELK FTVRDCNSIP 120

NIPGSCKETF NLFYYEADSD VASASSPFWM ENPYVKVDTI APDESFSRLD AGRVNTKVRS 180

FGPLSKAGFY LAFQDQGACM SLISVRAFYK KCASTTAGFA LFPETLTGAE PTSLVIAPGT 240

CIPNAVEVSV PLKLYCNGDG EWMVPVGACT CATGHEPAAK ESQCRPCPPG SYKAKQGEGP 300

CLPCPPNSRT TSPAASICTC HNNFYRADSD SADSACTTVP SPPRGVISNV NETSLILEWS 360

EPRDLGGRDD LLYNVICKKC HGAGGASACS RCDDNVEFVP RQLGLTERRV HISHLLAHTR 420

YTFEVQAVNG VSGKSPLPPR YAAVNITTNQ AAPSEVPTLR LHSSSGSSLT LSWAPPERPN 480

GVILDYEMKY FEKSEGIAST VTSQMNSVQL DGLRPDARYV VQVRARTVAG YGQYSRPAEF 540

ETTSERGSGA QQLQEQLPLI VGSATAGLVF VVAVVVIAIV CLRKQRHGSD SEYTEKLQQY 600

IAPGMKVYID PFTYEDPNEA VREFAKEIDV SCVKIEEVIG AGEFGEVCRG RLKQPGRREV 660

FVAIKTLKVG YTERQRRDFL SEASIMGQFD HPNIIRLEGV VTKSRPVMIL TEFMENCALD 720

SFLRLNDGQF TVIQLVGMLR GIAAGMKYLS EMNYVHRDLA ARNILVNSNL VCKVSDFGLS 780

RFLEDDPSDP TYTSSLGGKI PIRWTAPEAI AYRKFTSASD VWSYGIVMWE VMSYGERPYW 840

DMSNQDVINA VEQDYRLPPP MDCPTALHQL MLDCWVRDRN LRPKFSQIVN TLDKLIRNAA 900

SLKVIASAQS GMSQPLLDRT VPDYTTFTTV GDWLDAIKMG RYKESFVSAG FASFDLVAQM 960

TAEDLLRIGV TLAGHQKKIL SSIQDMRLQM NQTLPVQV 998

Nucleotide Sequence
(FASTA)

ATGGCCAGAG CCCGCCCGCC GCCGCCGCCG TCGCCGCCGC CGGGGCTTCT GCCGCTGCTC 60

CCTCCGCTGC TGCTGCTGCC GCTGCTGCTG CTGCCCGCCG GCTGCCGGGC GCTGGAAGAG 120

ACCCTCATGG ACACAAAATG GGTAACATCT GAGTTGGCGT GGACATCTCA TCCAGAAAGT 180

GGGTGGGAAG AGGTGAGTGG CTACGATGAG GCCATGAATC CCATCCGCAC ATACCAGGTG 240

TGTAATGTGC GCGAGTCAAG CCAGAACAAC TGGCTTCGCA CGGGGTTCAT CTGGCGGCGG 300

GATGTGCAGC GGGTCTACGT GGAGCTCAAG TTCACTGTGC GTGACTGCAA CAGCATCCCC 360

AACATCCCCG GCTCCTGCAA GGAGACCTTC AACCTCTTCT ACTACGAGGC TGACAGCGAT 420

GTGGCCTCAG CCTCCTCCCC CTTCTGGATG GAGAACCCCT ACGTGAAAGT GGACACCATT 480

GCACCCGATG AGAGCTTCTC GCGGCTGGAT GCCGGCCGTG TCAACACCAA GGTGCGCAGC 540

TTTGGGCCAC TTTCCAAGGC TGGCTTCTAC CTGGCCTTCC AGGACCAGGG CGCCTGCATG 600

TCGCTCATCT CCGTGCGCGC CTTCTACAAG AAGTGTGCAT CCACCACCGC AGGCTTCGCA 660

CTCTTCCCCG AGACCCTCAC TGGGGCGGAG CCCACCTCGC TGGTCATTGC TCCTGGCACC 720

TGCATCCCTA ACGCCGTGGA GGTGTCGGTG CCACTCAAGC TCTACTGCAA CGGCGATGGG 780

GAGTGGATGG TGCCTGTGGG TGCCTGCACC TGTGCCACCG GCCATGAGCC AGCTGCCAAG 840

GAGTCCCAGT GCCGCCCCTG TCCCCCTGGG AGCTACAAGG CGAAGCAGGG AGAGGGGCCC 900

TGCCTCCCAT GTCCCCCCAA CAGCCGTACC ACCTCCCCAG CCGCCAGCAT CTGCACCTGC 960

CACAATAACT TCTACCGTGC AGACTCGGAC TCTGCGGACA GTGCCTGTAC CACCGTGCCA 1020

TCTCCACCCC GAGGTGTGAT CTCCAATGTG AATGAAACCT CACTGATCCT CGAGTGGAGT 1080

GAGCCCCGGG ACCTGGGTGG CCGGGATGAC CTCCTGTACA ATGTCATCTG CAAGAAGTGC 1140

CATGGGGCTG GAGGGGCCTC AGCCTGCTCA CGCTGTGATG ACAACGTGGA GTTTGTGCCT 1200

CGGCAGCTGG GCCTGACGGA GCGCCGGGTC CACATCAGCC ATCTGCTGGC CCACACGCGC 1260

TACACCTTTG AGGTGCAGGC GGTCAACGGT GTCTCGGGCA AGAGCCCTCT GCCGCCTCGT 1320

TATGCGGCCG TGAATATCAC CACAAACCAG GCTGCCCCGT CTGAAGTGCC CACACTACGC 1380

CTGCACAGCA GCTCAGGCAG CAGCCTCACC CTATCCTGGG CACCCCCAGA GCGGCCCAAC 1440

GGAGTCATCC TGGACTACGA GATGAAGTAC TTTGAGAAGA GCGAGGGCAT CGCCTCCACA 1500

GTGACCAGCC AGATGAACTC CGTGCAGCTG GACGGGCTTC GGCCTGACGC CCGCTATGTG 1560

GTCCAGGTCC GTGCCCGCAC AGTAGCTGGC TATGGGCAGT ACAGCCGCCC TGCCGAGTTT 1620

GAGACCACAA GTGAGAGAGG CTCTGGGGCC CAGCAGCTCC AGGAGCAGCT TCCCCTCATC 1680

GTGGGCTCCG CTACAGCTGG GCTTGTCTTC GTGGTGGCTG TCGTGGTCAT CGCTATCGTC 1740

TGCCTCAGGA AGCAGCGACA CGGCTCTGAT TCGGAGTACA CGGAGAAGCT GCAGCAGTAC 1800

ATTGCTCCTG GAATGAAGGT TTATATTGAC CCTTTTACCT ACGAGGACCC TAATGAGGCT 1860

GTTCGGGAGT TTGCCAAGGA GATCGACGTG TCCTGCGTCA AGATCGAGGA GGTGATCGGA 1920

GCTGGGGAAT TTGGGGAAGT GTGCCGTGGT CGACTGAAAC AGCCTGGCCG CCGAGAGGTG 1980

TTTGTGGCCA TCAAGACGCT GAAGGTGGGC TACACCGAGA GGCAGCGGCG GGACTTCCTA 2040

AGCGAGGCCT CCATCATGGG TCAGTTTGAT CACCCCAATA TAATCCGGCT CGAGGGCGTG 2100

GTCACCAAAA GTCGGCCAGT TATGATCCTC ACTGAGTTCA TGGAAAACTG CGCCCTGGAC 2160

TCCTTCCTCC GGCTCAACGA TGGGCAGTTC ACGGTCATCC AGCTGGTGGG CATGTTGCGG 2220

GGCATTGCTG CCGGCATGAA GTACCTGTCC GAGATGAACT ATGTGCACCG CGACCTGGCT 2280

GCTCGCAACA TCCTTGTCAA CAGCAACCTG GTCTGCAAAG TCTCAGACTT TGGCCTCTCC 2340

CGCTTCCTGG AGGATGACCC CTCCGATCCT ACCTACACCA GTTCCCTGGG CGGGAAGATC 2400

CCCATCCGCT GGACTGCCCC AGAGGCCATA GCCTATCGGA AGTTCACTTC TGCTAGTGAT 2460

GTCTGGAGCT ACGGAATTGT CATGTGGGAG GTCATGAGCT ATGGAGAGCG ACCCTACTGG 2520

GACATGAGCA ACCAGGATGT CATCAATGCC GTGGAGCAGG ATTACCGGCT GCCACCACCC 2580

ATGGACTGTC CCACAGCACT GCACCAGCTC ATGCTGGACT GCTGGGTGCG GGACCGGAAC 2640

CTCAGGCCCA AATTCTCCCA GATTGTCAAT ACCCTGGACA AGCTCATCCG CAATGCTGCC 2700

AGCCTCAAGG TCATTGCCAG CGCTCAGTCT GGCATGTCAC AGCCCCTCCT GGACCGCACG 2760

GTCCCAGATT ACACAACCTT CACGACAGTT GGTGATTGGC TGGATGCCAT CAAGATGGGG 2820

CGGTACAAGG AGAGCTTCGT CAGTGCGGGG TTTGCATCTT TTGACCTGGT GGCCCAGATG 2880

ACGGCAGAAG ACCTGCTCCG TATTGGGGTC ACCCTGGCCG GCCACCAGAA GAAGATCCTG 2940

AGCAGTATCC AGGACATGCG GCTGCAGATG AACCAGACGC TGCCTGTGCA GGTCTGA 2997

Domain Profile

S: 1     vkiekviGaGefGevcsGrlklpgkreiavaiktlkagstekqrrdflseasilGqfdhp  60

         vkie+viGaGefGevc+Grlk+pg+re+ vaiktlk g+te+qrrdflseasi+Gqfdhp

Q: 633   VKIEEVIGAGEFGEVCRGRLKQPGRREVFVAIKTLKVGYTERQRRDFLSEASIMGQFDHP  692

         79**********************************************************

S: 61    niirleGvvtkskpvmivteymengaldsflrkkdgqftviqlvgmlrGiasgmkylsdm  120

         niirleGvvtks+pvmi+te+men+aldsflr +dgqftviqlvgmlrGia+gmkyls+m

Q: 693   NIIRLEGVVTKSRPVMILTEFMENCALDSFLRLNDGQFTVIQLVGMLRGIAAGMKYLSEM  752

         ************************************************************

S: 121   gyvhrdlaarnilvnsnlvckvsdfGlsrvledds.eaaytts.ggkipirwtapeaiay  178

         +yvhrdlaarnilvnsnlvckvsdfGlsr+ledd+ +++yt+s ggkipirwtapeaiay

Q: 753   NYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDPsDPTYTSSlGGKIPIRWTAPEAIAY  812

         **********************************99*****999****************

S: 179   rkftsasdvwsyGivlwevlsyGerpywdmsnqdvikaieegyrlpppmdcpaalyqlml  238

         rkftsasdvwsyGiv+wev+syGerpywdmsnqdvi+a+e++yrlpppmdcp+al+qlml

Q: 813   RKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVINAVEQDYRLPPPMDCPTALHQLML  872

         ************************************************************

S: 239   dcwqkdraerpkfeqivsil  258

         dcw +dr+ rpkf+qiv++l

Q: 873   DCWVRDRNLRPKFSQIVNTL  892

         *****************975

Domain Sequence
(FASTA)

VKIEEVIGAG EFGEVCRGRL KQPGRREVFV AIKTLKVGYT ERQRRDFLSE ASIMGQFDHP 60

NIIRLEGVVT KSRPVMILTE FMENCALDSF LRLNDGQFTV IQLVGMLRGI AAGMKYLSEM 120

NYVHRDLAAR NILVNSNLVC KVSDFGLSRF LEDDPSDPTY TSSLGGKIPI RWTAPEAIAY 180

RKFTSASDVW SYGIVMWEVM SYGERPYWDM SNQDVINAVE QDYRLPPPMD CPTALHQLML 240

DCWVRDRNLR PKFSQIVNTL 260

Keyword

3D-structure; Angiogenesis; ATP-binding; Cell membrane; Cell projection; Complete proteome; Developmental protein; Disulfide bond; Glycoprotein; Kinase; Membrane; Neurogenesis; Nucleotide-binding; Phosphoprotein; Polymorphism; Receptor; Reference proteome; Repeat; Signal; Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0030425	; C:dendrite
GO:0005887	; C:integral to plasma membrane
GO:0005524	; F:ATP binding
GO:0008046	; F:axon guidance receptor activity
GO:0005003	; F:ephrin receptor activity
GO:0001525	; P:angiogenesis
GO:0007411	; P:axon guidance
GO:0007413	; P:axonal fasciculation
GO:0016477	; P:cell migration
GO:0021952	; P:central nervous system projection neuron axonogenesis
GO:0022038	; P:corpus callosum development
GO:0060997	; P:dendritic spine morphogenesis
GO:0048546	; P:digestive tract morphogenesis
GO:0060021	; P:palate development
GO:0051965	; P:positive regulation of synapse assembly
GO:0046777	; P:protein autophosphorylation
GO:0050770	; P:regulation of axonogenesis
GO:0043088	; P:regulation of Cdc42 GTPase activity
GO:0022407	; P:regulation of cell-cell adhesion
GO:0032314	; P:regulation of Rac GTPase activity
GO:0031290	; P:retinal ganglion cell axon guidance
GO:0034446	; P:substrate adhesion-dependent cell spreading
GO:0048538	; P:thymus development
GO:0001655	; P:urogenital system development

KEGG

hsa:2049

;

InterPros

IPR001090	; Ephrin_rcpt_lig-bd_dom.
IPR003961	; Fibronectin_type3.
IPR008979	; Galactose-bd-like.
IPR013783	; Ig-like_fold.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001660	; SAM.
IPR013761	; SAM/pointed.
IPR021129	; SAM_type1.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR011641	; Tyr-kin_ephrin_A/B_rcpt-like.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.
IPR016257	; Tyr_kinase_ephrin_rcpt.
IPR001426	; Tyr_kinase_rcpt_V_CS.

Pfam

PF01404	; Ephrin_lbd; 1.
PF00041	; fn3; 2.
PF07699	; GCC2_GCC3; 1.
PF07714	; Pkinase_Tyr; 1.
PF00536	; SAM_1; 1.

SMARTs

SM00615	; EPH_lbd; 1.
SM00060	; FN3; 2.
SM00454	; SAM; 1.
SM00219	; TyrKc; 1.

Prosites

PS51550	; EPH_LBD; 1.
PS50853	; FN3; 2.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS00790	; RECEPTOR_TYR_KIN_V_1; 1.
PS00791	; RECEPTOR_TYR_KIN_V_2; 1.
PS50105	; SAM_DOMAIN; 1.

Prints

PR00109

; TYRKINASE.

Created Date

20-Feb-2013