| Tag | Content |
|---|
| EKPD ID | EKS-HOS-00376 |
| Classification | | Group/Family | Score | E-Value | Start | End | Domain Length |
|---|
| TK/Src | 434.1 | 7.4E-130 | 245 | 494 | 250 |
|
| Status | Reviewed |
| Ensembl Protein | ENSP00000337825 |
| UniProt Accession | P06239; D3DPP8; P07100; Q12850; Q13152; Q5TDH8; Q5TDH9; Q7RTZ3; Q96DW4; Q9NYT8; |
| Protein Name | Tyrosine-protein kinase Lck |
| Protein Synonyms/Alias | Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK; |
| Gene Name | LCK |
| Gene Synonyms/Alias | LCK; |
| Ensembl Information | |
| Organism | Homo sapiens |
| Functional Description | Non-receptor tyrosine-protein kinase that plays anessential role in the selection and maturation of developing T- cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP. |
| Protein Length | 509 |
Protein Sequence
(FASTA) | | MGCGCSSHPE DDWMENIDVC ENCHYPIVPL DGKGTLLIRN GSEVRDPLVT YEGSNPPASP 60 | | LQDNLVIALH SYEPSHDGDL GFEKGEQLRI LEQSGEWWKA QSLTTGQEGF IPFNFVAKAN 120 | | SLEPEPWFFK NLSRKDAERQ LLAPGNTHGS FLIRESESTA GSFSLSVRDF DQNQGEVVKH 180 | | YKIRNLDNGG FYISPRITFP GLHELVRHYT NASDGLCTRL SRPCQTQKPQ KPWWEDEWEV 240 | | PRETLKLVER LGAGQFGEVW MGYYNGHTKV AVKSLKQGSM SPDAFLAEAN LMKQLQHQRL 300 | | VRLYAVVTQE PIYIITEYME NGSLVDFLKT PSGIKLTINK LLDMAAQIAE GMAFIEERNY 360 | | IHRDLRAANI LVSDTLSCKI ADFGLARLIE DNEYTAREGA KFPIKWTAPE AINYGTFTIK 420 | | SDVWSFGILL TEIVTHGRIP YPGMTNPEVI QNLERGYRMV RPDNCPEELY QLMRLCWKER 480 | | PEDRPTFDYL RSVLEDFFTA TEGQYQPQP 509 |
|
Nucleotide Sequence
(FASTA) | | ATGGGCTGTG GCTGCAGCTC ACACCCGGAA GATGACTGGA TGGAAAACAT CGATGTGTGT 60 | | GAGAACTGCC ATTATCCCAT AGTCCCACTG GATGGCAAGG GCACGCTGCT CATCCGAAAT 120 | | GGCTCTGAGG TGCGGGACCC ACTGGTTACC TACGAAGGCT CCAATCCGCC GGCTTCCCCA 180 | | CTGCAAGACA ACCTGGTTAT CGCTCTGCAC AGCTATGAGC CCTCTCACGA CGGAGATCTG 240 | | GGCTTTGAGA AGGGGGAACA GCTCCGCATC CTGGAGCAGA GCGGCGAGTG GTGGAAGGCG 300 | | CAGTCCCTGA CCACGGGCCA GGAAGGCTTC ATCCCCTTCA ATTTTGTGGC CAAAGCGAAC 360 | | AGCCTGGAGC CCGAACCCTG GTTCTTCAAG AACCTGAGCC GCAAGGACGC GGAGCGGCAG 420 | | CTCCTGGCGC CCGGGAACAC TCACGGCTCC TTCCTCATCC GGGAGAGCGA GAGCACCGCG 480 | | GGATCGTTTT CACTGTCGGT CCGGGACTTC GACCAGAACC AGGGAGAGGT GGTGAAACAT 540 | | TACAAGATCC GTAATCTGGA CAACGGTGGC TTCTACATCT CCCCTCGAAT CACTTTTCCC 600 | | GGCCTGCATG AACTGGTCCG CCATTACACC AATGCTTCAG ATGGGCTGTG CACACGGTTG 660 | | AGCCGCCCCT GCCAGACCCA GAAGCCCCAG AAGCCGTGGT GGGAGGACGA GTGGGAGGTT 720 | | CCCAGGGAGA CGCTGAAGCT GGTGGAGCGG CTGGGGGCTG GACAGTTCGG GGAGGTGTGG 780 | | ATGGGGTACT ACAACGGGCA CACGAAGGTG GCGGTGAAGA GCCTGAAGCA GGGCAGCATG 840 | | TCCCCGGACG CCTTCCTGGC CGAGGCCAAC CTCATGAAGC AGCTGCAACA CCAGCGGCTG 900 | | GTTCGGCTCT ACGCTGTGGT CACCCAGGAG CCCATCTACA TCATCACTGA ATACATGGAG 960 | | AATGGGAGTC TAGTGGATTT TCTCAAGACC CCTTCAGGCA TCAAGTTGAC CATCAACAAA 1020 | | CTCCTGGACA TGGCAGCCCA AATTGCAGAA GGCATGGCAT TCATTGAAGA GCGGAATTAT 1080 | | ATTCATCGTG ACCTTCGGGC TGCCAACATT CTGGTGTCTG ACACCCTGAG CTGCAAGATT 1140 | | GCAGACTTTG GCCTAGCACG CCTCATTGAG GACAACGAGT ACACAGCCAG GGAGGGGGCC 1200 | | AAGTTTCCCA TTAAGTGGAC AGCGCCAGAA GCCATTAACT ACGGGACATT CACCATCAAG 1260 | | TCAGATGTGT GGTCTTTTGG GATCCTGCTG ACGGAAATTG TCACCCACGG CCGCATCCCT 1320 | | TACCCAGGGA TGACCAACCC GGAGGTGATT CAGAACCTGG AGCGAGGCTA CCGCATGGTG 1380 | | CGCCCTGACA ACTGTCCAGA GGAGCTGTAC CAACTCATGA GGCTGTGCTG GAAGGAGCGC 1440 | | CCAGAGGACC GGCCCACCTT TGACTACCTG CGCAGTGTGC TGGAGGACTT CTTCACGGCC 1500 | | ACAGAGGGCC AGTACCAGCC TCAGCCTTGA 1530 |
|
| Domain Profile | S: 1 lklvkklGeGqfGevwlgkwkgsvkvavktlkegtlspeafleeaqilkklrheklvkly 60 | lklv++lG+GqfGevw+g+++g++kvavk+lk+g++sp+afl+ea+++k+l+h++lv+ly | Q: 245 LKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHQRLVRLY 304 | 6899******************************************************** |
| S: 61 avvseeePiyivtelmakGslldflkeeegkklklpklvdlaaqvaeGmayleeknlihr 120 | avv++ ePiyi+te+m++Gsl+dflk+ +g kl+++kl+d+aaq+aeGma++ee+n+ihr | Q: 305 AVVTQ-EPIYIITEYMENGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFIEERNYIHR 363 | ****9.****************************************************** |
| S: 121 dlaarnvlvgeslvvkvadfGlarlieddeytakegaklPikWtaPeaiklgkftiksdv 180 | dl+a+n+lv+++l +k+adfGlarlied+eyta+egak+PikWtaPeai++g+ftiksdv | Q: 364 DLRAANILVSDTLSCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIKSDV 423 | ************************************************************ |
| S: 181 WsfGillteivtkGkvPypGmtneevleqvergyrlprpeecpeelyelllecwkkdpee 240 | WsfGillteivt+G++PypGmtn ev++++ergyr+ rp++cpeely+l+ cwk++pe+ | Q: 424 WSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYQLMRLCWKERPED 483 | ************************************************************ |
| S: 241 rPtfetlqevl 251 | rPtf++l++vl | Q: 484 RPTFDYLRSVL 494 | *******9876 |
|
Domain Sequence
(FASTA) | | LKLVERLGAG QFGEVWMGYY NGHTKVAVKS LKQGSMSPDA FLAEANLMKQ LQHQRLVRLY 60 | | AVVTQEPIYI ITEYMENGSL VDFLKTPSGI KLTINKLLDM AAQIAEGMAF IEERNYIHRD 120 | | LRAANILVSD TLSCKIADFG LARLIEDNEY TAREGAKFPI KWTAPEAINY GTFTIKSDVW 180 | | SFGILLTEIV THGRIPYPGM TNPEVIQNLE RGYRMVRPDN CPEELYQLMR LCWKERPEDR 240 | | PTFDYLRSVL 250 |
|
| Keyword | 3D-structure; Acetylation; Alternative splicing; ATP-binding; Cell membrane; Chromosomal rearrangement; Complete proteome; Cytoplasm; Disease mutation; Host-virus interaction; Kinase; Lipoprotein; Membrane; Myristate; Nucleotide-binding; Palmitate; Phosphoprotein; Polymorphism; Proto-oncogene; Reference proteome; SH2 domain; SH3 domain; Transferase; Tyrosine-protein kinase. |
| Sequence Source | Ensembl |
| Orthology | |
| Gene Ontology | |
| KEGG | |
| InterPros | |
| Pfam | |
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| Prints | |
| Created Date | 20-Feb-2013 |