EKS-HOS-00423

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00423

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CAMK/DAPK	478.8	2.0E-143	14	275	262

Status

Reviewed

Ensembl Protein

ENSP00000419026

UniProt Accession

P53355; B7ZLD2; Q14CQ7; Q1W5W0; Q68CP8; Q6ZRZ3; Q9BTL8;

Protein Name

Death-associated protein kinase 1

Protein Synonyms/Alias

DAP kinase 1;

Gene Name

DAPK1

Gene Synonyms/Alias

DAPK1; DAPK;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000196730	ENSP00000417076	ENST00000472284
ENSG00000196730	ENSP00000350785	ENST00000358077
ENSG00000196730	ENSP00000418885	ENST00000469640
ENSG00000196730	ENSP00000419026	ENST00000491893
ENSG00000196730	ENSP00000417746	ENST00000489291
ENSG00000196730	ENSP00000386135	ENST00000408954

Organism

Homo sapiens

Functional Description

Calcium/calmodulin-dependent serine/threonine kinaseinvolved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca(2+) influx through NMDA receptor channels, resulting in an irreversible neuronal death. Isoform 2 cannot induce apoptosis but can inducemembrane blebbing.

Protein Length

1364

Protein Sequence
(FASTA)

MTVFRQENVD DYYDTGEELG SGQFAVVKKC REKSTGLQYA AKFIKKRRTK SSRRGVSRED 60

IEREVSILKE IQHPNVITLH EVYENKTDVI LILELVAGGE LFDFLAEKES LTEEEATEFL 120

KQILNGVYYL HSLQIAHFDL KPENIMLLDR NVPKPRIKII DFGLAHKIDF GNEFKNIFGT 180

PEFVAPEIVN YEPLGLEADM WSIGVITYIL LSGASPFLGD TKQETLANVS AVNYEFEDEY 240

FSNTSALAKD FIRRLLVKDP KKRMTIQDSL QHPWIKPKDT QQALSRKASA VNMEKFKKFA 300

ARKKWKQSVR LISLCQRLSR SFLSRSNMSV ARSDDTLDEE DSFVMKAIIH AINDDNVPGL 360

QHLLGSLSNY DVNQPNKHGT PPLLIAAGCG NIQILQLLIK RGSRIDVQDK GGSNAVYWAA 420

RHGHVDTLKF LSENKCPLDV KDKSGEMALH VAARYGHADV AQLLCSFGSN PNIQDKEEET 480

PLHCAAWHGY YSVAKALCEA GCNVNIKNRE GETPLLTASA RGYHDIVECL AEHGADLNAC 540

DKDGHIALHL AVRRCQMEVI KTLLSQGCFV DYQDRHGNTP LHVACKDGNM PIVVALCEAN 600

CNLDISNKYG RTPLHLAANN GILDVVRYLC LMGASVEALT TDGKTAEDLA RSEQHEHVAG 660

LLARLRKDTH RGLFIQQLRP TQNLQPRIKL KLFGHSGSGK TTLVESLKCG LLRSFFRRRR 720

PRLSSTNSSR FPPSPLASKP TVSVSINNLY PGCENVSVRS RSMMFEPGLT KGMLEVFVAP 780

THHPHCSADD QSTKAIDIQN AYLNAFGGKL KNPLQVVLVA THADIMNVPR PAGGEFGYDK 840

DTSLLKEIRN RFGNDLHISN KLFVLDAGAS GSKDMKVLRN HLQEIRSQIV SVCPPMTHLC 900

EKIISTLPSW RKLNGPNQLM SLQQFVYDVQ DQLNPLASEE DLRRIAQQLH STGEINIMQS 960

ETVQDVLLLD PRWLCTNVLG KLLSVETPRA LHHYRGRYTV EDIQRLVPDS DVEELLQILD 1020

AMDICARDLS SGTMVDVPAL IKTDNLHRSW ADEEDEVMVY GGVRIVPVEH LTPFPCGIFH 1080

KVQVNLCRWI HQQSTEGDAD IRLWVNGCKL ANRGAELLVL LVNHGQGIEV QVRGLETEKI 1140

KCCLLLDSVC STIENVMATT LPGLLTVKHY LSPQQLREHH EPVMIYQPRD FFRAQTLKET 1200

SLTNTMGGYK ESFSSIMCFG CHDVYSQASL GMDIHASDLN LLTRRKLSRL LDPPDPLGKD 1260

WCLLAMNLGL PDLVAKYNTS NGAPKDFLPS PLHALLREWT TYPESTVGTL MSKLRELGRR 1320

DAADFLLKAS SVFKINLDGN GQEAYASSCN SGTSYNSISS VVSR 1364

Nucleotide Sequence
(FASTA)

ATGACCGTGT TCAGGCAGGA AAACGTGGAT GATTACTACG ACACCGGCGA GGAACTTGGC 60

AGTGGACAGT TTGCGGTTGT GAAGAAATGC CGTGAGAAAA GCACCGGCCT CCAGTATGCC 120

GCCAAATTCA TCAAGAAAAG GAGGACTAAG TCCAGCCGGC GGGGTGTGAG CCGCGAGGAC 180

ATCGAGCGGG AGGTCAGCAT CCTGAAGGAG ATCCAGCACC CCAATGTCAT CACCCTGCAC 240

GAGGTCTATG AGAACAAGAC GGACGTCATC CTGATCTTGG AACTCGTTGC AGGTGGCGAG 300

CTGTTTGACT TCTTAGCTGA AAAGGAATCT TTAACTGAAG AGGAAGCAAC TGAATTTCTC 360

AAACAAATTC TTAATGGTGT TTACTACCTG CACTCCCTTC AAATCGCCCA CTTTGATCTT 420

AAGCCTGAGA ACATAATGCT TTTGGATAGA AATGTCCCCA AACCTCGGAT CAAGATCATT 480

GACTTTGGGT TGGCCCATAA AATTGACTTT GGAAATGAAT TTAAAAACAT ATTTGGGACT 540

CCAGAGTTTG TCGCTCCTGA GATAGTCAAC TATGAACCTC TTGGTCTTGA GGCAGATATG 600

TGGAGTATCG GGGTAATAAC CTATATCCTC CTAAGTGGGG CCTCCCCATT TCTTGGAGAC 660

ACTAAGCAAG AAACGTTAGC AAATGTATCC GCTGTCAACT ACGAATTTGA GGATGAATAC 720

TTCAGTAATA CCAGTGCCCT AGCCAAAGAT TTCATAAGAA GACTTCTGGT CAAGGATCCA 780

AAGAAGAGAA TGACAATTCA AGATAGTTTG CAGCATCCCT GGATCAAGCC TAAAGATACA 840

CAACAGGCAC TTAGTAGAAA AGCATCAGCA GTAAACATGG AGAAATTCAA GAAGTTTGCA 900

GCCCGGAAAA AATGGAAACA ATCCGTTCGC TTGATATCAC TGTGCCAAAG ATTATCCAGG 960

TCATTCCTGT CCAGAAGTAA CATGAGTGTT GCCAGAAGCG ATGATACTCT GGATGAGGAA 1020

GACTCCTTTG TGATGAAAGC CATCATCCAT GCCATCAACG ATGACAATGT CCCAGGCCTG 1080

CAGCACCTTC TGGGCTCATT ATCCAACTAT GATGTTAACC AACCCAACAA GCACGGGACA 1140

CCTCCATTAC TCATTGCTGC TGGCTGTGGG AATATTCAAA TACTACAGTT GCTCATTAAA 1200

AGAGGCTCGA GAATCGATGT CCAGGATAAG GGCGGGTCCA ATGCCGTCTA CTGGGCTGCT 1260

CGGCATGGCC ACGTCGATAC CTTGAAATTT CTCAGTGAGA ACAAATGCCC TTTGGATGTG 1320

AAAGACAAGT CTGGAGAGAT GGCCCTCCAC GTGGCAGCTC GCTATGGCCA TGCTGACGTG 1380

GCTCAGTTAC TGTGCAGCTT CGGCTCAAAT CCCAATATCC AGGACAAGGA AGAAGAAACC 1440

CCCCTGCACT GTGCTGCTTG GCACGGCTAT TACTCTGTGG CCAAAGCCCT TTGTGAAGCC 1500

GGCTGTAACG TGAACATCAA GAACCGAGAA GGAGAGACGC CCCTCCTGAC AGCCTCTGCC 1560

AGGGGCTACC ACGACATCGT GGAGTGTCTG GCCGAACATG GAGCCGACCT TAATGCTTGC 1620

GACAAGGACG GACACATTGC CCTTCATCTG GCTGTAAGAC GGTGTCAGAT GGAGGTAATC 1680

AAGACTCTCC TCAGCCAAGG GTGTTTCGTC GATTATCAAG ACAGGCACGG CAATACTCCC 1740

CTCCATGTGG CATGTAAAGA TGGCAACATG CCTATCGTGG TGGCCCTCTG TGAAGCAAAC 1800

TGCAATTTGG ACATCTCCAA CAAGTATGGG CGAACGCCTC TGCACCTTGC GGCCAACAAC 1860

GGAATCCTAG ACGTGGTCCG GTATCTCTGT CTGATGGGAG CCAGCGTTGA GGCGCTGACC 1920

ACGGACGGAA AGACGGCAGA AGATCTTGCT AGATCGGAAC AGCACGAGCA CGTAGCAGGT 1980

CTCCTTGCAA GACTTCGAAA GGATACGCAC CGAGGACTCT TCATCCAGCA GCTCCGACCC 2040

ACACAGAACC TGCAGCCAAG AATTAAGCTC AAGCTGTTTG GCCACTCGGG ATCCGGGAAA 2100

ACCACCCTTG TAGAATCTCT CAAGTGTGGG CTGCTGAGGA GCTTTTTCAG AAGGCGTCGG 2160

CCCAGACTGT CTTCCACCAA CTCCAGCAGG TTCCCACCTT CACCCCTGGC TTCTAAGCCC 2220

ACAGTCTCAG TGAGCATCAA CAACCTGTAC CCAGGCTGCG AGAACGTGAG TGTGAGGAGC 2280

CGCAGCATGA TGTTCGAGCC GGGTCTTACC AAAGGGATGC TGGAGGTGTT TGTGGCCCCG 2340

ACCCACCACC CGCACTGCTC GGCCGATGAC CAGTCCACCA AGGCCATCGA CATCCAGAAC 2400

GCTTATTTGA ATGCCTTCGG TGGCAAGCTG AAGAACCCAC TCCAAGTTGT CCTGGTGGCC 2460

ACCCACGCTG ACATCATGAA TGTTCCTCGA CCGGCTGGAG GCGAGTTTGG ATATGACAAA 2520

GACACATCGT TGCTGAAAGA GATTAGGAAC AGGTTTGGAA ATGATCTTCA CATTTCAAAT 2580

AAGCTGTTTG TTCTGGATGC TGGGGCTTCT GGGTCAAAGG ACATGAAGGT ACTTCGAAAT 2640

CATCTGCAAG AAATACGAAG CCAGATTGTT TCGGTCTGTC CTCCCATGAC TCACCTGTGT 2700

GAGAAAATCA TCTCCACGCT GCCTTCCTGG AGGAAGCTCA ATGGACCCAA CCAGCTGATG 2760

TCGCTGCAGC AGTTTGTGTA CGACGTGCAG GACCAGCTGA ACCCCCTGGC CAGCGAGGAG 2820

GACCTCAGGC GCATTGCTCA GCAGCTCCAC AGCACAGGCG AGATCAACAT CATGCAAAGT 2880

GAAACAGTTC AGGACGTGCT GCTCCTGGAC CCCCGCTGGC TCTGCACAAA CGTCCTGGGG 2940

AAGTTGCTGT CCGTGGAGAC CCCACGGGCG CTGCACCACT ACCGGGGCCG CTACACCGTG 3000

GAGGACATCC AGCGCCTGGT GCCCGACAGC GACGTGGAGG AGCTGCTGCA GATCCTCGAT 3060

GCCATGGACA TCTGCGCCCG GGACCTGAGC AGCGGGACCA TGGTGGACGT CCCAGCCCTG 3120

ATCAAGACAG ACAACCTGCA CCGCTCCTGG GCTGATGAGG AGGACGAGGT GATGGTGTAT 3180

GGTGGCGTGC GCATCGTGCC CGTGGAACAC CTCACCCCCT TCCCATGTGG CATCTTTCAC 3240

AAGGTCCAGG TGAACCTGTG CCGGTGGATC CACCAGCAAA GCACAGAGGG CGACGCGGAC 3300

ATCCGCCTGT GGGTGAATGG CTGCAAGCTG GCCAACCGTG GGGCCGAGCT GCTGGTGCTG 3360

CTGGTCAACC ACGGCCAGGG CATTGAGGTC CAGGTCCGCG GCCTGGAGAC GGAGAAGATC 3420

AAGTGCTGCC TGCTGCTGGA CTCGGTGTGC AGCACCATTG AGAACGTCAT GGCCACCACG 3480

CTGCCAGGGC TCCTGACCGT GAAGCATTAC CTGAGCCCCC AGCAGCTGCG GGAGCACCAT 3540

GAGCCCGTCA TGATCTACCA GCCACGGGAC TTCTTCCGGG CACAGACTCT GAAGGAAACC 3600

TCACTGACCA ACACCATGGG GGGGTACAAG GAAAGCTTCA GCAGCATCAT GTGCTTCGGG 3660

TGTCACGACG TCTACTCACA GGCCAGCCTC GGCATGGACA TCCATGCATC AGACCTGAAC 3720

CTCCTCACTC GGAGGAAACT GAGTCGCCTG CTGGACCCGC CCGACCCCCT GGGGAAGGAC 3780

TGGTGCCTTC TCGCCATGAA CTTAGGCCTC CCTGACCTCG TGGCAAAGTA CAACACCAGT 3840

AACGGGGCTC CCAAGGATTT CCTCCCCAGC CCCCTCCACG CCCTGCTGCG GGAATGGACC 3900

ACCTACCCTG AGAGCACAGT GGGCACCCTC ATGTCCAAAC TGAGGGAGCT GGGTCGCCGG 3960

GATGCCGCAG ACTTTTTGCT GAAGGCATCC TCTGTGTTCA AAATCAACCT GGATGGCAAT 4020

GGCCAGGAGG CCTATGCCTC GAGCTGCAAC AGCGGCACCT CTTACAATTC CATTAGCTCT 4080

GTTGTATCCC GGTGA 4095

Domain Profile

S: 2     etgkelgsGkfavvrkcrekstGkeyaakflkkrrlkssrrgvsreeierevavlkeirs  61

         +tg+elgsG+favv+kcrekstG +yaakf+kkrr+kssrrgvsre+ierev++lkei+

Q: 14    DTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILKEIQ-  72

         789********************************************************.

S: 62    epnvinlhevyenksdvililelvaGGelfdllaekeslseeeatellkqilegvkylhs  121

         +pnvi+lhevyenk+dvililelvaGGelfd+laekesl+eeeate+lkqil+gv+ylhs

Q: 73    HPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILNGVYYLHS  132

         ************************************************************

S: 122   kkivhldlkPenillldkkvpkgdiklidfGlsrkieegaelkeilGtPefvaPeivnye  181

          +i+h+dlkPeni+lld++vpk++ik+idfGl++ki+ g+e+k+i+GtPefvaPeivnye

Q: 133   LQIAHFDLKPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYE  192

         ************************************************************

S: 182   plsletdmwsiGvityillsgasPflGdtkqetllnisavnldfseelfssvselakdfi  241

         pl+le+dmwsiGvityillsgasPflGdtkqetl+n+savn++f++e+fs++s lakdfi

Q: 193   PLGLEADMWSIGVITYILLSGASPFLGDTKQETLANVSAVNYEFEDEYFSNTSALAKDFI  252

         ************************************************************

S: 242   rrllvkdpekrltieeclehswl  264

         rrllvkdp+kr+ti+++l+h+w+

Q: 253   RRLLVKDPKKRMTIQDSLQHPWI  275

         **********************8

Domain Sequence
(FASTA)

DTGEELGSGQ FAVVKKCREK STGLQYAAKF IKKRRTKSSR RGVSREDIER EVSILKEIQH 60

PNVITLHEVY ENKTDVILIL ELVAGGELFD FLAEKESLTE EEATEFLKQI LNGVYYLHSL 120

QIAHFDLKPE NIMLLDRNVP KPRIKIIDFG LAHKIDFGNE FKNIFGTPEF VAPEIVNYEP 180

LGLEADMWSI GVITYILLSG ASPFLGDTKQ ETLANVSAVN YEFEDEYFSN TSALAKDFIR 240

RLLVKDPKKR MTIQDSLQHP WI 262

Keyword

3D-structure; Alternative splicing; ANK repeat; Apoptosis; ATP-binding; Calmodulin-binding; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0015629	; C:actin cytoskeleton
GO:0005737	; C:cytoplasm
GO:0005524	; F:ATP binding
GO:0005516	; F:calmodulin binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0097190	; P:apoptotic signaling pathway
GO:0006917	; P:induction of apoptosis
GO:0007243	; P:intracellular protein kinase cascade
GO:0046777	; P:protein autophosphorylation
GO:0010506	; P:regulation of autophagy
GO:2000310	; P:regulation of N-methyl-D-aspartate selective glutamate receptor activity

KEGG

hsa:1612

;

InterPros

IPR002110	; Ankyrin_rpt.
IPR020683	; Ankyrin_rpt-contain_dom.
IPR000488	; Death.
IPR020676	; Death-assoc_prot_kinase.
IPR011029	; DEATH-like.
IPR011009	; Kinase-like_dom.
IPR020675	; Myosin_light_ch_kinase-rel.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00023	; Ank; 6.
PF00531	; Death; 1.
PF00069	; Pkinase; 1.

SMARTs

SM00248	; ANK; 9.
SM00005	; DEATH; 1.
SM00220	; S_TKc; 1.

Prosites

PS50297	; ANK_REP_REGION; 1.
PS50088	; ANK_REPEAT; 7.
PS50017	; DEATH_DOMAIN; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

PR01415

; ANKYRIN.

Created Date

20-Feb-2013