EKS-HOS-00164

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00164

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/TLK	616.2	3.4E-185	360	638	279

Status

Reviewed

Ensembl Protein

ENSP00000409222

UniProt Accession

Q9UKI8; B3KR15; B4DX87; Q14150; Q8N591; Q9NYH2; Q9Y4F6;

Protein Name

Serine/threonine-protein kinase tousled-like 1

Protein Synonyms/Alias

PKU-beta; Tousled-like kinase 1;

Gene Name

TLK1

Gene Synonyms/Alias

TLK1; KIAA0137;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000198586	ENSP00000402165	ENST00000442919
ENSG00000198586	ENSP00000354089	ENST00000360843
ENSG00000198586	ENSP00000387313	ENST00000409443
ENSG00000198586	ENSP00000428113	ENST00000521943
ENSG00000198586	ENSP00000393165	ENST00000453628
ENSG00000198586	ENSP00000397910	ENST00000413010
ENSG00000198586	ENSP00000411099	ENST00000431350
ENSG00000198586	ENSP00000409222	ENST00000434911
ENSG00000198586	ENSP00000352810	ENST00000359766

Organism

Homo sapiens

Functional Description

Rapidly and transiently inhibited by phosphorylationfollowing the generation of DNA double-stranded breaks during S- phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by faciliting the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'.

Protein Length

670

Protein Sequence
(FASTA)

MAVLFLYDLK TTGKTPEKKQ SESSRGRKRK AENQNESSQG KSIGGRGHKI SDYFEYQGGN 60

GSSPVRGIPP AIRSPQNSHS HSTPSSSVRP NSPSPTALAF GDHPIVQPKQ LSFKIIQTDL 120

TMLKLAALES NKIQDLEKKE GRIDDLLRAN CDLRRQIDEQ QKLLEKYKER LNKCISMSKK 180

LLIEKSTQEK LSSREKSMQD RLRLGHFTTV RHGASFTEQW TDGFAFQNLV KQQEWVNQQR 240

EDIERQRKLL AKRKPPTANN SQAPSTNSEP KQRKNKAVNG AENDPFVRPN LPQLLTLAEY 300

HEQEEIFKLR LGHLKKEEAE IQAELERLER VRNLHIRELK RINNEDNSQF KDHPTLNERY 360

LLLHLLGRGG FSEVYKAFDL YEQRYAAVKI HQLNKSWRDE KKENYHKHAC REYRIHKELD 420

HPRIVKLYDY FSLDTDTFCT VLEYCEGNDL DFYLKQHKLM SEKEARSIVM QIVNALRYLN 480

EIKPPIIHYD LKPGNILLVD GTACGEIKIT DFGLSKIMDD DSYGVDGMDL TSQGAGTYWY 540

LPPECFVVGK EPPKISNKVD VWSVGVIFFQ CLYGRKPFGH NQSQQDILQE NTILKATEVQ 600

FPVKPVVSSE AKAFIRRCLA YRKEDRFDVH QLANDPYLLP HMRRSNSSGN LHMAGLTASP 660

TPPSSSIITY 670

Nucleotide Sequence
(FASTA)

ATGGCTGTTT TATTCTTGTA TGATTTGAAA ACTACTGGCA AGACACCGGA GAAGAAACAA 60

TCGGAATCAT CCAGGGGAAG AAAGAGAAAA GCAGAAAACC AGAATGAAAG TAGTCAGGGA 120

AAAAGTATTG GGGGACGTGG CCACAAAATT AGCGACTATT TTGAATACCA GGGTGGAAAT 180

GGCTCAAGTC CAGTAAGAGG CATACCTCCT GCAATCCGTT CTCCTCAAAA TTCACATTCA 240

CATTCCACTC CTTCCTCATC TGTTCGACCG AATAGCCCTT CTCCTACTGC ATTAGCATTT 300

GGGGACCACC CTATTGTACA ACCAAAGCAA TTATCCTTTA AAATTATTCA GACTGATCTC 360

ACAATGCTGA AATTAGCAGC ATTAGAAAGT AATAAAATCC AGGACCTGGA AAAGAAGGAG 420

GGACGTATAG ATGATTTGCT CAGGGCTAAC TGTGATCTCA GACGGCAAAT AGATGAACAA 480

CAAAAATTAC TTGAAAAATA CAAAGAACGA TTAAATAAGT GCATATCAAT GAGCAAGAAA 540

CTTCTTATTG AAAAGAGTAC ACAAGAAAAG CTGTCAAGCA GAGAGAAGAG TATGCAAGAT 600

CGATTACGCC TCGGGCACTT TACAACAGTT AGACATGGCG CTTCATTTAC TGAACAATGG 660

ACAGATGGTT TTGCATTTCA GAATCTTGTG AAGCAACAAG AATGGGTGAA TCAGCAAAGG 720

GAAGATATTG AAAGGCAAAG GAAACTTCTA GCCAAACGCA AACCTCCCAC AGCTAATAAT 780

TCTCAGGCAC CCTCTACCAA TTCTGAACCA AAACAAAGGA AAAACAAAGC AGTCAATGGA 840

GCAGAGAATG ATCCCTTTGT TAGACCAAAT TTACCACAAC TGTTGACTTT GGCAGAATAT 900

CATGAACAGG AAGAAATTTT CAAACTTAGA CTAGGACATC TCAAAAAGGA AGAGGCAGAA 960

ATCCAGGCAG AACTTGAACG TTTGGAAAGA GTCAGAAATC TTCACATACG TGAGCTGAAA 1020

AGAATAAACA ATGAAGATAA TTCACAGTTC AAAGATCACC CAACATTAAA TGAAAGATAT 1080

TTATTACTTC ATCTGCTTGG TAGAGGTGGC TTTAGTGAAG TGTATAAGGC TTTTGACCTT 1140

TATGAACAAA GATATGCTGC TGTGAAGATA CATCAGCTTA ATAAAAGCTG GAGAGATGAG 1200

AAGAAAGAAA ACTACCACAA ACATGCCTGC AGAGAGTATA GAATACACAA AGAACTGGAT 1260

CACCCCAGAA TAGTTAAACT CTATGATTAT TTCTCCTTGG ATACAGATAC GTTTTGTACA 1320

GTGTTAGAAT ACTGTGAAGG CAATGACTTG GATTTCTATC TGAAGCAACA CAAGTTAATG 1380

TCAGAGAAAG AAGCTCGGTC TATTGTAATG CAGATTGTAA ATGCACTAAG ATATCTCAAT 1440

GAGATCAAAC CCCCTATTAT ACATTATGAT CTTAAGCCAG GAAACATCCT ACTGGTAGAT 1500

GGAACAGCAT GTGGTGAAAT CAAAATCACT GATTTTGGTC TGTCCAAGAT TATGGATGAT 1560

GATAGCTATG GTGTAGATGG AATGGATCTA ACTTCCCAGG GGGCAGGCAC TTACTGGTAT 1620

TTACCTCCTG AGTGTTTTGT AGTTGGAAAA GAGCCACCAA AGATTTCCAA CAAGGTTGAT 1680

GTATGGTCGG TTGGAGTCAT CTTCTTTCAG TGTCTTTATG GTAGAAAGCC ATTTGGTCAC 1740

AATCAATCTC AACAAGACAT TCTTCAAGAA AATACAATAT TAAAAGCCAC AGAAGTCCAG 1800

TTCCCTGTAA AACCGGTTGT AAGCAGTGAA GCCAAGGCAT TTATAAGACG CTGTTTGGCA 1860

TATCGAAAAG AAGATCGATT TGATGTGCAC CAGCTGGCAA ATGACCCATA CCTTCTCCCA 1920

CACATGAGAA GATCAAATTC TTCAGGAAAC CTACACATGG CTGGGCTGAC AGCATCCCCT 1980

ACACCCCCTT CTTCAAGCAT AATTACTTAC TGA 2013

Domain Profile

S: 1     ylllkllgkggfsevykafdlkeqryvavkihqlnkdwkdekkenyvkhalreyrihkel  60

         ylll+llg+ggfsevykafdl+eqry+avkihqlnk+w+dekkeny+kha+reyrihkel

Q: 360   YLLLHLLGRGGFSEVYKAFDLYEQRYAAVKIHQLNKSWRDEKKENYHKHACREYRIHKEL  419

         9***********************************************************

S: 61    dhprivklydllsldtdsfctvleyvegndldfylkqhkllsekearsiimqivsalkyl  120

         dhprivklyd++sldtd+fctvley+egndldfylkqhkl+sekearsi+mqiv+al+yl

Q: 420   DHPRIVKLYDYFSLDTDTFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIVMQIVNALRYL  479

         ************************************************************

S: 121   neikppiihydlkpgnillvegtacgeikitdfglskimdddsydsddgleltsqgagty  180

         neikppiihydlkpgnillv+gtacgeikitdfglskimdddsy++d g++ltsqgagty

Q: 480   NEIKPPIIHYDLKPGNILLVDGTACGEIKITDFGLSKIMDDDSYGVD-GMDLTSQGAGTY  538

         *********************************************99.************

S: 181   wylppecfvvgkeppkisnkvdvwsvgvifyqclygkkpfghnqsqqdileentilkate  240

         wylppecfvvgkeppkisnkvdvwsvgvif+qclyg+kpfghnqsqqdil+entilkate

Q: 539   WYLPPECFVVGKEPPKISNKVDVWSVGVIFFQCLYGRKPFGHNQSQQDILQENTILKATE  598

         ************************************************************

S: 241   vqfpskpvvsseakafirrclayrkedridvqqlakdeyl  280

         vqfp+kpvvsseakafirrclayrkedr+dv+qla+d+yl

Q: 599   VQFPVKPVVSSEAKAFIRRCLAYRKEDRFDVHQLANDPYL  638

         **************************************97

Domain Sequence
(FASTA)

YLLLHLLGRG GFSEVYKAFD LYEQRYAAVK IHQLNKSWRD EKKENYHKHA CREYRIHKEL 60

DHPRIVKLYD YFSLDTDTFC TVLEYCEGND LDFYLKQHKL MSEKEARSIV MQIVNALRYL 120

NEIKPPIIHY DLKPGNILLV DGTACGEIKI TDFGLSKIMD DDSYGVDGMD LTSQGAGTYW 180

YLPPECFVVG KEPPKISNKV DVWSVGVIFF QCLYGRKPFG HNQSQQDILQ ENTILKATEV 240

QFPVKPVVSS EAKAFIRRCL AYRKEDRFDV HQLANDPYL 279

Keyword

Alternative splicing; ATP-binding; Cell cycle; Chromatin regulator; Coiled coil; Complete proteome; DNA damage; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0007049	; P:cell cycle
GO:0016568	; P:chromatin modification
GO:0006886	; P:intracellular protein transport
GO:0035556	; P:intracellular signal transduction
GO:0001672	; P:regulation of chromatin assembly or disassembly
GO:0006974	; P:response to DNA damage stimulus

KEGG

hsa:9874

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013