EKS-HOS-00393

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-HOS-00393

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
STE/STE11	370.8	2.4E-110	358	617	260

Status

Reviewed

Ensembl Protein

ENSP00000462086

UniProt Accession

Q99759; B2RCW2; D3DU15; Q5BKZ6; Q8N3I9;

Protein Name

Mitogen-activated protein kinase kinase kinase 3

Protein Synonyms/Alias

MAPK/ERK kinase kinase 3; MEK kinase 3; MEKK 3;

Gene Name

MAP3K3

Gene Synonyms/Alias

MAP3K3; MAPKKK3, MEKK3;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSG00000198909	ENSP00000464482	ENST00000581732
ENSG00000198909	ENSP00000464532	ENST00000585302
ENSG00000198909	ENSP00000354927	ENST00000361357
ENSG00000198909	ENSP00000354485	ENST00000361733
ENSG00000198909	ENSP00000462086	ENST00000577395
ENSG00000198909	ENSP00000462917	ENST00000577597
ENSG00000198909	ENSP00000464130	ENST00000584573
ENSG00000198909	ENSP00000461988	ENST00000579585

Organism

Homo sapiens

Functional Description

Component of a protein kinase signal transductioncascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.

Protein Length

622

Protein Sequence
(FASTA)

MDEQEALNSI MNDLVALQMN RRHRMPGYET MKNKDTGHSN RQSDVRIKFE HNGERRIIAF 60

SRPVKYEDVE HKVTTVFGQP LDLHYMNNEL SILLKNQDDL DKAIDILDRS SSMKSLRILL 120

LSQDRNHNSS SPHSGVSRQV RIKASQSAGD INTIYQPPEP RSRHLSVSSQ NPGRSSPPPG 180

YVPERQQHIA RQGSYTSINS EGEFIPETSE QCMLDPLSSA ENSLSGSCQS LDSPSFRKSR 240

MSRAQSFPDN RQEYSDRETQ LYDKGVKGGT YPRRYHVSVH HKDYSDGRRT FPRIRRHQGN 300

LFTLVPSSRS LSTNGENMGL AVQYLDPRGR LRSADSENAL SVQERNVPTK SPSAPINWRR 360

GKLLGQGAFG RVYLCYDVDT GRELASKQVQ FDPDSPETSK EVSALECEIQ LLKNLQHERI 420

VQYYGCLRDR AEKTLTIFME YMPGGSVKDQ LKAYGALTES VTRKYTRQIL EGMSYLHSNM 480

IVHRDIKGAN ILRDSAGNVK LGDFGASKRL QTICMSGTGM RSVTGTPYWM SPEVISGEGY 540

GRKADVWSLG CTVVEMLTEK PPWAEYEAMA AIFKIATQPT NPQLPSHISE HGRDFLRRIF 600

VEARQRPSAE ELLTHHFAQL MY 622

Nucleotide Sequence
(FASTA)

ATGGACGAAC AGGAGGCATT GAACTCAATC ATGAACGATC TGGTGGCCCT CCAGATGAAC 60

CGACGTCACC GGATGCCTGG ATATGAGACC ATGAAGAACA AAGACACAGG TCACTCAAAT 120

AGGCAGAGTG ACGTCAGAAT CAAGTTCGAG CACAACGGGG AGAGGCGAAT TATAGCGTTC 180

AGCCGGCCTG TGAAATATGA AGATGTGGAG CACAAGGTGA CAACAGTATT TGGACAACCT 240

CTTGATCTAC ATTACATGAA CAATGAGCTC TCCATCCTGC TGAAAAACCA AGATGATCTT 300

GATAAAGCAA TTGACATTTT AGATAGAAGC TCAAGCATGA AAAGCCTTAG GATATTGCTG 360

TTGTCCCAGG ACAGAAACCA TAACAGTTCC TCTCCCCACT CTGGGGTGTC CAGACAGGTG 420

CGGATCAAGG CTTCCCAGTC CGCAGGGGAT ATAAATACTA TCTACCAGCC CCCCGAGCCC 480

AGAAGCAGGC ACCTCTCTGT CAGCTCCCAG AACCCTGGCC GAAGCTCACC TCCCCCTGGC 540

TATGTTCCTG AGCGGCAGCA GCACATTGCC CGGCAGGGGT CCTACACCAG CATCAACAGT 600

GAGGGGGAGT TCATCCCAGA GACCAGCGAG CAGTGCATGC TGGATCCCCT GAGCAGTGCA 660

GAAAATTCCT TGTCTGGAAG CTGCCAATCC TTGGACAGCC CATCCTTCCG GAAATCACGA 720

ATGTCCCGTG CCCAGAGCTT CCCTGACAAC AGACAGGAAT ACTCAGATCG GGAAACTCAG 780

CTTTATGACA AAGGGGTCAA AGGTGGAACC TACCCCCGGC GCTACCACGT GTCTGTGCAC 840

CACAAGGACT ACAGTGATGG CAGAAGAACA TTTCCCCGAA TACGGCGTCA TCAAGGCAAC 900

TTGTTCACCC TGGTGCCCTC CAGCCGCTCC CTGAGCACAA ATGGCGAGAA CATGGGTCTG 960

GCTGTGCAAT ACCTGGACCC CCGTGGGCGC CTGCGGAGTG CGGACAGCGA GAATGCCCTC 1020

TCTGTGCAGG AGAGGAATGT GCCAACCAAG TCTCCCAGTG CCCCCATCAA CTGGCGCCGG 1080

GGAAAGCTCC TGGGCCAGGG TGCCTTCGGC AGGGTCTATT TGTGCTATGA CGTGGACACG 1140

GGACGTGAAC TTGCTTCCAA GCAGGTCCAA TTTGATCCAG ACAGTCCTGA GACAAGCAAG 1200

GAGGTGAGTG CTCTGGAGTG CGAGATCCAG TTGCTAAAGA ACTTGCAGCA TGAGCGCATC 1260

GTGCAGTACT ATGGCTGTCT GCGGGACCGC GCTGAGAAGA CCCTGACCAT CTTCATGGAG 1320

TACATGCCAG GGGGCTCGGT GAAAGACCAG TTGAAGGCTT ACGGTGCTCT GACAGAGAGC 1380

GTGACCCGAA AGTACACGCG GCAGATCCTG GAGGGCATGT CCTACCTGCA CAGCAACATG 1440

ATTGTTCACC GGGACATTAA GGGAGCCAAC ATCCTCCGAG ACTCTGCTGG GAATGTAAAG 1500

CTGGGGGACT TTGGGGCCAG CAAACGCCTG CAGACGATCT GTATGTCGGG GACGGGCATG 1560

CGCTCCGTCA CTGGCACACC CTACTGGATG AGCCCTGAGG TGATCAGCGG CGAGGGCTAT 1620

GGAAGGAAAG CAGACGTGTG GAGCCTGGGC TGCACTGTGG TGGAGATGCT GACAGAGAAA 1680

CCACCGTGGG CAGAGTATGA AGCTATGGCC GCCATCTTCA AGATTGCCAC CCAGCCCACC 1740

AATCCTCAGC TGCCCTCCCA CATCTCTGAA CATGGCCGGG ACTTCCTGAG GCGCATTTTT 1800

GTGGAGGCTC GCCAGAGACC TTCAGCTGAG GAGCTGCTCA CACACCACTT TGCACAGCTC 1860

ATGTACTGA 1869

Domain Profile

S: 1     wqkrevigkGsfgsvyaaldvdtgellavKeveikpekksekkevkalkeEikllkelkH  60

         w++++++g+G+fg+vy+++dvdtg+ la K+v+++p++ +++kev+al++Ei+llk+l+H

Q: 358   WRRGKLLGQGAFGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSALECEIQLLKNLQH  417

         899*********************************************************

S: 61    enivkyyGv..eveeeelkiflEyvegGslssllekygkleesvikkytkqileglayLH  118

         e+iv+yyG+  ++ e++l+if+Ey++gGs++++l++yg+l+esv++kyt+qileg++yLH

Q: 418   ERIVQYYGClrDRAEKTLTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYLH  477

         *********77778999*******************************************

S: 119   ekkivHRDiKgaNilldssgvvklsDFGaskrlaelntkaaelesltGtlaymaPEvikk  178

         +++ivHRDiKgaNil ds+g+vkl+DFGaskrl++++++ + ++s+tGt+++m+PEvi++

Q: 478   SNMIVHRDIKGANILRDSAGNVKLGDFGASKRLQTICMSGTGMRSVTGTPYWMSPEVISG  537

         **********************************************************99

S: 179   kekgygrkaDiWslgcvviemltgkrPfaeldeelaimfkvgakktkPeipeklseegkd  238

         +  gygrkaD+Wslgc+v+emlt+k+P+ae+ e++a++fk++++ t+P++p+++se+g+d

Q: 538   E--GYGRKADVWSLGCTVVEMLTEKPPWAEY-EAMAAIFKIATQPTNPQLPSHISEHGRD  594

         8..****************************.9***************************

S: 239   flekcleldpkkrpsaeeLLkhef  262

         fl++++ +++++rpsaeeLL+h+f

Q: 595   FLRRIF-VEARQRPSAEELLTHHF  617

         ******.8999***********99

Domain Sequence
(FASTA)

WRRGKLLGQG AFGRVYLCYD VDTGRELASK QVQFDPDSPE TSKEVSALEC EIQLLKNLQH 60

ERIVQYYGCL RDRAEKTLTI FMEYMPGGSV KDQLKAYGAL TESVTRKYTR QILEGMSYLH 120

SNMIVHRDIK GANILRDSAG NVKLGDFGAS KRLQTICMSG TGMRSVTGTP YWMSPEVISG 180

EGYGRKADVW SLGCTVVEML TEKPPWAEYE AMAAIFKIAT QPTNPQLPSH ISEHGRDFLR 240

RIFVEARQRP SAEELLTHHF 260

Keyword

3D-structure; Alternative splicing; ATP-binding; Complete proteome; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tupaia belangeri"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005829	; C:cytosol
GO:0005524	; F:ATP binding
GO:0004709	; F:MAP kinase kinase kinase activity
GO:0046872	; F:metal ion binding
GO:0043123	; P:positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0046777	; P:protein autophosphorylation

KEGG

hsa:4215

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000270	; OPR_PB1.
IPR000719	; Prot_kinase_cat_dom.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.

Pfam

PF00564	; PB1; 1.
PF00069	; Pkinase; 1.

SMARTs

SM00666	; PB1; 1.
SM00220	; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; FALSE_NEG.

Prints

Created Date

20-Feb-2013