EKS-GAG-00042

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-GAG-00042

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TKL/RAF	412.9	6.9E-124	349	606	258

Status

Reviewed

Ensembl Protein

ENSGALP00000007991

UniProt Accession

P05625; Q90893; Q90894;

Protein Name

RAF proto-oncogene serine/threonine-protein kinase

Protein Synonyms/Alias

C-MIL; C-RAF; MIL proto-oncogene serine/threonine-protein kinase; RAF-1;

Gene Name

RAF1

Gene Synonyms/Alias

RAF1; MIL;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSGALG00000004998	ENSGALP00000033314	ENSGALT00000033956
ENSGALG00000004998	ENSGALP00000007991	ENSGALT00000008005

Organism

Gallus gallus

Functional Description

Serine/threonine-protein kinase that acts as aregulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2) (By similarity).

Protein Length

647

Protein Sequence
(FASTA)

MEHIQGAWKT ISNGFGLKDS VFDGPNCISP TIVQQFGYQR RASDDGKISD TSKTSNTIRV 60

FLPNKQRTVV NVRNGMTLHD CLMKALKVRG LQPECCAVFR LVTEPKGKKV RLDWNTDAAS 120

LIGEELQVDF LDHVPLTTHN FARKTFLKLA FCDICQKFLL NGFRCQTCGY KFHEHCSTKV 180

PTMCVDWSNI RQLLLFPNSN ISDSGVPALP PLTMRRMRES VSRIPVSSQH RYSTPHVFTF 240

NTSNPSSEGT LSQRQRSTST PNVHMVSTTM PVDSRIIEDA IRNHSESASP SALSGSPNNM 300

SPTGWSQPKT PVPAQRERAP GTNTQEKNKI RPRGQRDSSY YWEIEASEVM LSTRIGSGSF 360

GTVYKGKWHG DVAVKILKVV DPTPEQFQAF RNEVAVLRKT RHVNILLFMG YMTKDNLAIV 420

TQWCEGSSLY KHLHVQETKF QMFQLIDIAR QTAQGMDYLH AKNIIHRDMK SNNIFLHEGL 480

TVKIGDFGLA TVKSRWSGSQ QVEQPTGSIL WMAPEVIRMQ DSNPFSFQSD VYSYGIVLYE 540

LMTGELPYSH INNRDQIIFM VGRGYASPDL SKLYKNCPKA MKRLVADCLK KVREERPLFP 600

QILSSIELLQ HSLPKINRSA SEPSLHRASH TEDINSCTLT STRLPVF 647

Nucleotide Sequence
(FASTA)

ATGGAGCACA TTCAGGGAGC TTGGAAGACT ATCAGTAATG GTTTTGGACT CAAGGATTCT 60

GTCTTTGATG GCCCAAACTG CATTTCACCG ACCATTGTCC AGCAGTTTGG TTATCAGCGC 120

CGAGCATCTG ATGATGGCAA GATATCAGAT ACTTCCAAAA CCAGTAATAC CATTCGAGTT 180

TTCTTGCCCA ATAAGCAACG TACAGTGGTA AATGTGCGAA ATGGGATGAC CTTACATGAT 240

TGTCTCATGA AGGCACTTAA AGTAAGAGGT CTGCAGCCAG AATGCTGTGC AGTTTTTCGT 300

CTTGTTACTG AACCAAAAGG TAAAAAAGTG CGTTTGGATT GGAACACTGA TGCTGCCTCC 360

TTGATTGGTG AGGAACTGCA GGTGGACTTT CTTGATCACG TTCCGCTCAC TACACACAAT 420

TTTGCTCGGA AGACATTTCT AAAGCTTGCT TTCTGTGACA TCTGCCAGAA GTTCCTCCTG 480

AATGGGTTTC GGTGTCAGAC GTGTGGTTAT AAATTCCACG AGCACTGCAG CACTAAAGTT 540

CCAACCATGT GCGTCGACTG GAGCAATATC AGGCAACTCT TATTGTTCCC AAATTCAAAT 600

ATCAGTGACA GTGGTGTCCC TGCACTACCT CCCTTGACAA TGAGACGGAT GCGGGAGTCT 660

GTCTCCCGGA TACCTGTTAG CTCCCAGCAC AGGTATTCTA CACCTCATGT CTTTACATTC 720

AACACATCAA ATCCTTCCTC TGAGGGCACC CTTTCCCAAA GACAGCGATC TACATCCACA 780

CCAAATGTCC ACATGGTTAG CACTACAATG CCAGTAGACA GCCGGATAAT TGAGGATGCA 840

ATTCGAAACC ATAGTGAATC AGCTTCACCC TCCGCTCTGT CTGGGAGTCC TAACAATATG 900

AGCCCGACTG GCTGGTCTCA GCCCAAAACG CCAGTCCCAG CCCAGAGGGA GAGAGCCCCC 960

GGAACGAATA CACAGGAGAA AAATAAAATT AGGCCTCGTG GACAAAGAGA TTCTAGTTAT 1020

TACTGGGAAA TAGAAGCAAG CGAAGTCATG CTTTCTACCA GAATAGGGTC AGGTTCTTTT 1080

GGAACTGTTT ACAAAGGCAA ATGGCATGGG GATGTAGCAG TGAAAATATT AAAGGTTGTA 1140

GATCCAACCC CAGAACAGTT TCAGGCTTTC AGAAACGAAG TGGCTGTATT AAGGAAGACC 1200

CGGCATGTTA ATATTTTGCT CTTCATGGGC TACATGACTA AAGATAACCT GGCCATTGTC 1260

ACACAGTGGT GTGAAGGCAG CAGTCTGTAT AAACACCTGC ACGTTCAAGA GACCAAGTTC 1320

CAAATGTTCC AGCTCATTGA CATTGCTCGG CAGACAGCGC AGGGAATGGA CTATTTGCAT 1380

GCAAAGAATA TCATCCACAG AGACATGAAA TCCAATAATA TATTTCTTCA TGAAGGCCTC 1440

ACAGTGAAAA TAGGAGACTT TGGTCTAGCA ACTGTAAAAT CCAGGTGGAG TGGATCGCAG 1500

CAGGTGGAGC AACCCACTGG TTCCATTTTG TGGATGGCAC CAGAAGTGAT ACGGATGCAA 1560

GACAGCAATC CGTTCAGTTT TCAGTCAGAT GTCTACTCCT ATGGAATAGT ATTGTATGAG 1620

CTAATGACAG GAGAGCTGCC ATACTCCCAC ATAAACAACC GCGACCAGAT TATTTTCATG 1680

GTTGGTCGAG GATATGCTTC TCCAGACCTC AGCAAGTTGT ACAAGAACTG CCCCAAAGCA 1740

ATGAAGAGGC TCGTAGCAGA TTGTTTGAAG AAAGTTAGGG AAGAAAGACC CTTGTTTCCG 1800

CAAATACTGT CTTCCATTGA ATTGCTGCAA CATTCTTTAC CCAAAATCAA CCGGAGTGCT 1860

TCCGAACCAT CTCTGCACCG CGCATCCCAT ACAGAGGACA TAAATTCTTG CACGTTAACA 1920

TCCACAAGAC TGCCTGTTTT TTAG 1944

Domain Profile

S: 1     velaekiGsGrfGtvyrgkwhGdvavkllnveelseeqleaFkkevaaykktRhenivLF  60

         v+l+++iGsG+fGtvy+gkwhGdvavk+l+v ++++eq++aF++eva+++ktRh+ni+LF

Q: 349   VMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLF  408

         57899*******************************************************

S: 61    mGyvskpelaivtslckgssLykllheqetkldlaklidiarqiaqgmdYLhakkilhkD  120

         mGy++k++laivt++c+gssLyk+lh+qetk+++ +lidiarq+aqgmdYLhak+i+h+D

Q: 409   MGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRD  468

         ************************************************************

S: 121   lksknilleeglkvvitDFgllsvkrllsgrrekqleipkgwilylapeviralsidleq  180

         +ks+ni+l+egl+v+i+DFgl++vk+++sg  ++q+e+p+g+il++apevir+      q

Q: 469   MKSNNIFLHEGLTVKIGDFGLATVKSRWSG--SQQVEQPTGSILWMAPEVIRM------Q  520

         ******************************..*********************......*

S: 181   delpfskesDvyafGivlyellarelpyke.esadqilfavgrG.lkpdlskvksklpke  238

         d++pfs++sDvy++Givlyel+++elpy++ +++dqi+f+vgrG ++pdlsk+++++pk+

Q: 521   DSNPFSFQSDVYSYGIVLYELMTGELPYSHiNNRDQIIFMVGRGyASPDLSKLYKNCPKA  580

         ************************************************************

S: 239   lkellleClkfekeeRPlftqilkkl  264

         +k+l+++Clk+ +eeRPlf+qil+++

Q: 581   MKRLVADCLKKVREERPLFPQILSSI  606

         **********************9875

Domain Sequence
(FASTA)

VMLSTRIGSG SFGTVYKGKW HGDVAVKILK VVDPTPEQFQ AFRNEVAVLR KTRHVNILLF 60

MGYMTKDNLA IVTQWCEGSS LYKHLHVQET KFQMFQLIDI ARQTAQGMDY LHAKNIIHRD 120

MKSNNIFLHE GLTVKIGDFG LATVKSRWSG SQQVEQPTGS ILWMAPEVIR MQDSNPFSFQ 180

SDVYSYGIVL YELMTGELPY SHINNRDQII FMVGRGYASP DLSKLYKNCP KAMKRLVADC 240

LKKVREERPL FPQILSSI 258

Keyword

Alternative splicing; ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Kinase; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Proto-oncogene; Reference proteome; Serine/threonine-protein kinase; Transferase; Zinc; Zinc-finger.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Macropus eugenii"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0005886	; C:plasma membrane
GO:0031143	; C:pseudopodium
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0005057	; F:receptor signaling protein activity
GO:0006915	; P:apoptotic process
GO:0007010	; P:cytoskeleton organization
GO:0035556	; P:intracellular signal transduction
GO:0043066	; P:negative regulation of apoptotic process
GO:0008285	; P:negative regulation of cell proliferation
GO:0031333	; P:negative regulation of protein complex assembly
GO:0048011	; P:nerve growth factor receptor signaling pathway
GO:0033138	; P:positive regulation of peptidyl-serine phosphorylation

KEGG

gga:396245

;

InterPros

IPR020454	; DAG/PE-bd.
IPR011009	; Kinase-like_dom.
IPR002219	; Prot_Kinase_C-like_PE/DAG-bd.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR003116	; Raf-like_ras-bd.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00130	; C1_1; 1.
PF07714	; Pkinase_Tyr; 1.
PF02196	; RBD; 1.

SMARTs

SM00109	; C1; 1.
SM00455	; RBD; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS50898	; RBD; 1.
PS00479	; ZF_DAG_PE_1; 1.
PS50081	; ZF_DAG_PE_2; 1.

Prints

PR00008

; DAGPEDOMAIN.

Created Date

20-Feb-2013