EKS-GAG-00359

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-GAG-00359

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/WEE	346.1	1.3E-103	217	489	273

Status

Reviewed

Ensembl Protein

ENSGALP00000020972

UniProt Accession

E1BTE1;

Protein Name

Wee1-like protein kinase 2

Protein Synonyms/Alias

Wee1-like protein kinase 1B; Wee1B kinase;

Gene Name

WEE2

Gene Synonyms/Alias

WEE2; WEE1B;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSGALG00000012875	ENSGALP00000020972	ENSGALT00000021002

Organism

Gallus gallus

Functional Description

Oocyte-specific protein tyrosine kinase thatphosphorylates and inhibits CDK1 and acts as a key regulator of meiosis. Required to maintain meiotic arrest in oocytes by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry (By similarity).

Protein Length

565

Protein Sequence
(FASTA)

MDDWENNDFI QRLDFSSCGE EGEDRSINEE DTLSSSPIKH CDFQKWNSPL PATPHRKLSE 60

IFLSRTKSWV SPTLKSSPGV SRTHGNAETP LHITWKKLQL CDTPHTPKSL LSKTAFPSPG 120

AKVPPKGFRH LRFTPGADLD DSTQASLVNI NPFTPESYRQ MLFLPNGKRK AREIKLVHLA 180

QSFCEKAEVQ RKDSAVRPAD RCPLKDSNMV SRYQKEFLEL ERIGVGEFGS VYKCIKRLDG 240

CVYAIKRSKR PLAGSSDEQL ALREVYAHAV LGHHPHVVRY YSAWAEDDHM IIQNEHCNGG 300

SLQDVLLENA KRGQYFPEAK LKEILLQVSM GLKYIHNSGL VHLDIKPSNI FICHKLVVEG 360

QAGQEESDSD DEFSSGVMYK IGDLGHVTSI ANPQVEEGDR RFLANEILQE QYFHLPKADI 420

FALALTIALA AGAGPLPHNG AMWHHIRKGN IPSIPQKLPN GFIELLKLMI HPDPMERPSA 480

TALTKHPILR PSLGKAVQLQ KQLNVEKCKT AMLERELKAA RLAQTLMKDQ PLGNANLQES 540

ETSPKKNNKR LVGGKNCRSF SFTVG 565

Nucleotide Sequence
(FASTA)

ATGGATGACT GGGAGAACAA CGACTTCATC CAGAGGCTGG ATTTTTCCAG CTGTGGAGAA 60

GAGGGTGAAG ACAGAAGCAT TAATGAGGAG GACACACTGA GTTCCAGCCC AATCAAGCAC 120

TGTGACTTCC AGAAGTGGAA CAGCCCTCTC CCTGCAACTC CTCACAGGAA GCTCAGTGAG 180

ATTTTCCTGA GCAGGACCAA ATCGTGGGTG AGCCCTACCC TGAAGTCCTC CCCAGGTGTG 240

AGCAGGACCC ATGGCAATGC AGAGACACCA CTGCACATCA CTTGGAAGAA GCTGCAGCTC 300

TGTGACACCC CCCACACTCC CAAGAGCCTG TTATCAAAGA CAGCTTTTCC TTCACCTGGG 360

GCAAAGGTCC CACCCAAAGG CTTCAGACAT CTGAGATTTA CTCCTGGTGC TGACTTGGAT 420

GACTCTACCC AAGCTTCTCT TGTCAACATC AATCCCTTTA CACCAGAGTC ATACCGACAA 480

ATGCTCTTTC TTCCTAATGG CAAGAGGAAA GCCAGAGAGA TCAAACTTGT CCACCTTGCA 540

CAATCATTTT GTGAGAAAGC TGAAGTACAG AGAAAGGACT CTGCTGTGAG ACCAGCAGAT 600

AGATGTCCTC TGAAAGACAG CAATATGGTT TCCCGCTACC AGAAGGAATT CCTGGAACTA 660

GAGAGGATTG GTGTAGGGGA ATTTGGTTCT GTCTACAAGT GTATCAAGCG CCTTGATGGA 720

TGTGTTTATG CCATCAAACG CTCCAAGAGG CCTCTGGCAG GATCCTCGGA TGAGCAGCTG 780

GCATTGCGTG AGGTTTATGC TCATGCAGTC CTTGGACACC ACCCCCATGT TGTGCGCTAC 840

TACTCAGCAT GGGCAGAAGA TGATCACATG ATTATTCAAA ATGAACATTG TAATGGTGGG 900

AGTCTCCAAG ATGTGCTTCT GGAAAATGCA AAGCGTGGCC AGTATTTCCC TGAAGCAAAG 960

CTGAAAGAAA TCTTGTTACA AGTCTCCATG GGACTAAAAT ACATCCACAA TTCTGGCCTT 1020

GTGCACTTGG ATATTAAACC TAGTAATATC TTCATCTGTC ACAAGCTGGT AGTTGAGGGC 1080

CAAGCTGGAC AGGAAGAGAG TGACAGTGAT GATGAGTTCT CTTCTGGTGT GATGTATAAG 1140

ATTGGTGACC TTGGCCACGT GACATCAATA GCAAACCCTC AAGTGGAGGA AGGAGACAGA 1200

CGGTTTTTAG CCAATGAGAT TTTGCAGGAG CAATACTTCC ATTTGCCAAA GGCAGACATC 1260

TTTGCCCTGG CGCTCACAAT AGCTCTGGCA GCAGGTGCGG GACCACTGCC TCACAATGGG 1320

GCCATGTGGC ACCACATCCG CAAAGGCAAT ATCCCATCAA TCCCTCAGAA GCTTCCCAAT 1380

GGCTTTATTG AACTCCTCAA GCTCATGATC CATCCTGATC CAATGGAAAG ACCCTCTGCC 1440

ACAGCTCTTA CGAAGCACCC AATCCTCCGT CCTTCACTTG GAAAAGCTGT GCAGCTCCAG 1500

AAGCAGCTAA ATGTGGAGAA GTGTAAGACT GCCATGCTGG AAAGGGAACT AAAAGCAGCT 1560

CGCCTTGCCC AGACCCTCAT GAAGGACCAG CCCTTAGGAA ATGCCAACTT ACAGGAGTCT 1620

GAGACCTCTC CAAAGAAGAA CAACAAGCGC CTGGTGGGAG GGAAGAATTG TCGTTCATTT 1680

AGCTTTACTG TGGGT 1695

Domain Profile

S: 1     FeevekiGkGefgevfkvvkredgklyAikkskkplkglskekkalrEvtahavlgqhen  60

         F e+e+iG+Gefg+v+k++kr+dg++yAik+sk+pl+g+s+e+ alrEv+ahavlg+h++

Q: 217   FLELERIGVGEFGSVYKCIKRLDGCVYAIKRSKRPLAGSSDEQLALREVYAHAVLGHHPH  276

         7899********************************************************

S: 61    vvryisaWeeddslyiqtElCeggsLeaalqeceakkkaleeaelkkilldlllglrylH  120

         vvry+saW+edd+++iq+E C+ggsL+++l e+ +  ++++ea+lk+ill+++ gl+y+H

Q: 277   VVRYYSAWAEDDHMIIQNEHCNGGSLQDVLLENAKRGQYFPEAKLKEILLQVSMGLKYIH  336

         ************************************************************

S: 121   ssslvHlDikpenifi.....................tkevtlklgDlGlvtelnsedks  159

         +s lvHlDikp+nifi                     ++ v++k+gDlG+vt++    ++

Q: 337   NSGLVHLDIKPSNIFIchklvvegqagqeesdsddefSSGVMYKIGDLGHVTSI---ANP  393

         ***********************************99999**************...99*

S: 160   kveEGDsrylakEilqeeyskl.kaDiFslgltilevaanvelPsnGeaWhklRkgklpd  218

         +veEGD+r+la+Eilqe+y +l kaDiF+l+lti+ +a++ +lP+nG +Wh++Rkg++p

Q: 394   QVEEGDRRFLANEILQEQYFHLpKADIFALALTIALAAGAGPLPHNGAMWHHIRKGNIP-  452

         ***********************************************************.

S: 219   eilkelsedlrellklmlepdperRptaeellkhevl  255

          i ++l + + ellklm++pdp +Rp+a++l kh++l

Q: 453   SIPQKLPNGFIELLKLMIHPDPMERPSATALTKHPIL  489

         **********************************986

Domain Sequence
(FASTA)

FLELERIGVG EFGSVYKCIK RLDGCVYAIK RSKRPLAGSS DEQLALREVY AHAVLGHHPH 60

VVRYYSAWAE DDHMIIQNEH CNGGSLQDVL LENAKRGQYF PEAKLKEILL QVSMGLKYIH 120

NSGLVHLDIK PSNIFICHKL VVEGQAGQEE SDSDDEFSSG VMYKIGDLGH VTSIANPQVE 180

EGDRRFLANE ILQEQYFHLP KADIFALALT IALAAGAGPL PHNGAMWHHI RKGNIPSIPQ 240

KLPNGFIELL KLMIHPDPME RPSATALTKH PIL 273

Keyword

ATP-binding; Coiled coil; Complete proteome; Kinase; Magnesium; Meiosis; Metal-binding; Nucleotide-binding; Nucleus; Reference proteome; Transferase; Tyrosine-protein kinase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Equus caballus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Ochotona princeps"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>

Gene Ontology

GO:0005813	; C:centrosome
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0000287	; F:magnesium ion binding
GO:0004715	; F:non-membrane spanning protein tyrosine kinase activity
GO:0004674	; F:protein serine/threonine kinase activity
GO:0007343	; P:egg activation
GO:0007143	; P:female meiosis
GO:0035038	; P:female pronucleus assembly
GO:0007137	; P:meiotic metaphase II
GO:0007128	; P:meiotic prophase I
GO:0007067	; P:mitosis
GO:0060283	; P:negative regulation of oocyte development
GO:0060631	; P:regulation of meiosis I

KEGG

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR008271	; Ser/Thr_kinase_AS.
IPR017164	; Wee1-like_protein_kinase.

Pfam

PF00069

; Pkinase; 1.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013