EKS-MEG-00040

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MEG-00040

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TKL/RAF	412.9	5.2E-124	349	606	258

Status

Unreviewed

Ensembl Protein

ENSMGAP00000004996

UniProt Accession

G1N0N1;

Protein Name

Protein Synonyms/Alias

Gene Name

RAF1

Gene Synonyms/Alias

RAF1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMGAG00000005082	ENSMGAP00000004996	ENSMGAT00000005721

Organism

Meleagris gallopavo

Functional Description

Protein Length

647

Protein Sequence
(FASTA)

MEHIQGAWKT ISNGFGLKDS VFDGPNCISP TIVQQFGYQR RASDDGKISD TSKTSNTIRV 60

FLPNKQRTVV NVRNGMTLHD CLMKALKVRG LQPECCAVFR LVTEPKGKKV RLDWNTDAAS 120

LIGEELQVDF LDHVPLTTHN FARKTFLKLA FCDICQKFLL NGFRCQTCGY KFHEHCSTKV 180

PTMCVDWSNI RQLLLFPNSN ISDSGVPALP PLTMRRMRES VSRIPVSSQH RYSTPHVFTF 240

NTSNPSSEGT LSQRQRSTST PNVHMVSTTM PVDSRIIEDA IRNHSESASP SALSGSPNNM 300

SPTGWSQPKT PVPAQRERAP GTNTQEKNKI RPRGQRDSSY YWEIEASEVM LSTRIGSGSF 360

GTVYKGKWHG DVAVKILKVV DPTPEQFQAF RNEVAVLRKT RHVNILLFMG YMTKDNLAIV 420

TQWCEGSSLY KHLHVQETKF QMFQLIDIAR QTAQGMDYLH AKNIIHRDMK SNNIFLHEGL 480

TVKIGDFGLA TVKSRWSGSQ QVEQPTGSIL WMAPEVIRMQ DSNPFSFQSD VYSYGIVLYE 540

LMTGELPYSH INNRDQIIFM VGRGYASPDL SKLYKNCPKA MKRLVADCLK KVREERPLFP 600

QILSSIELLQ HSLPKINRSA SEPSLHRASH TEDINSCTLT STRLPVF 647

Nucleotide Sequence
(FASTA)

ATGGAGCACA TTCAGGGAGC TTGGAAGACT ATCAGTAATG GTTTTGGACT CAAGGATTCT 60

GTCTTCGATG GCCCAAACTG CATTTCACCA ACCATTGTCC AGCAGTTTGG TTATCAGCGC 120

CGAGCATCTG ATGATGGCAA AATATCAGAT ACTTCTAAAA CCAGCAATAC CATTCGAGTT 180

TTCTTGCCCA ACAAGCAACG TACAGTGGTA AATGTGCGAA ATGGGATGAC CTTACATGAT 240

TGTCTCATGA AGGCACTTAA AGTAAGAGGT TTGCAGCCAG AATGCTGTGC AGTTTTTCGT 300

CTTGTTACTG AACCAAAAGG TAAAAAAGTG CGTTTGGATT GGAACACTGA TGCTGCCTCC 360

TTGATTGGTG AAGAACTGCA GGTGGACTTT CTTGATCATG TTCCACTCAC TACACACAAT 420

TTTGCTCGGA AGACATTTCT AAAGCTTGCT TTCTGTGACA TCTGCCAGAA GTTCCTCCTA 480

AATGGGTTTC GGTGTCAGAC GTGCGGTTAT AAATTCCACG AGCACTGCAG CACTAAAGTT 540

CCAACCATGT GTGTCGACTG GAGCAATATC AGGCAACTCT TATTGTTCCC AAATTCAAAT 600

ATCAGTGACA GTGGTGTCCC TGCACTACCT CCCTTGACAA TGAGACGGAT GCGGGAGTCT 660

GTCTCCCGGA TACCTGTTAG CTCCCAGCAC AGGTATTCTA CACCTCATGT CTTTACATTC 720

AACACATCAA ATCCTTCCTC TGAGGGCACC CTTTCCCAAA GACAGCGATC TACATCCACA 780

CCAAATGTCC ACATGGTTAG CACTACAATG CCAGTTGACA GCCGGATAAT TGAGGATGCA 840

ATTCGAAATC ATAGTGAATC AGCTTCACCC TCTGCTCTGT CTGGAAGTCC TAACAATATG 900

AGCCCGACTG GCTGGTCTCA GCCCAAAACC CCAGTCCCAG CCCAGAGAGA GCGAGCCCCT 960

GGGACAAATA CACAAGAAAA AAACAAAATT AGGCCTCGTG GACAAAGAGA TTCTAGTTAT 1020

TACTGGGAAA TAGAAGCAAG TGAAGTCATG CTTTCTACCA GAATAGGGTC AGGTTCTTTT 1080

GGAACTGTTT ACAAGGGCAA ATGGCATGGG GATGTAGCAG TGAAAATACT AAAAGTTGTA 1140

GATCCAACCC CAGAACAGTT TCAGGCTTTC AGAAATGAAG TGGCTGTATT AAGGAAGACT 1200

CGGCATGTTA ATATTTTGCT CTTCATGGGC TACATGACTA AAGATAACCT GGCCATTGTC 1260

ACACAGTGGT GTGAAGGCAG CAGTCTGTAT AAACACCTGC ACGTTCAAGA GACCAAGTTC 1320

CAAATGTTCC AGCTCATTGA CATTGCTCGG CAGACAGCAC AGGGAATGGA CTATTTACAT 1380

GCAAAGAATA TCATCCACAG GGACATGAAA TCCAATAATA TATTTCTTCA TGAAGGCCTC 1440

ACAGTGAAAA TAGGAGACTT TGGTCTAGCA ACTGTAAAAT CCAGGTGGAG TGGATCGCAA 1500

CAGGTGGAGC AACCCACTGG TTCCATTTTG TGGATGGCAC CAGAAGTGAT ACGGATGCAA 1560

GACAGTAATC CGTTCAGTTT CCAGTCAGAT GTCTACTCCT ATGGAATAGT ACTGTATGAG 1620

CTAATGACAG GAGAGCTGCC ATACTCCCAC ATAAACAACC GCGACCAGAT TATTTTCATG 1680

GTTGGCCGAG GATATGCTTC TCCAGACCTC AGCAAGTTGT ACAAGAACTG CCCCAAAGCA 1740

ATGAAGAGGC TTGTAGCAGA TTGTTTGAAG AAAGTTAGGG AAGAAAGACC TTTGTTTCCG 1800

CAAATACTGT CTTCCATTGA ATTGCTGCAA CATTCTTTAC CCAAAATCAA CCGGAGCGCT 1860

TCCGAACCAT CTCTGCACCG CGCATCCCAT ACAGAGGACA TAAATTCTTG CACGTTAACA 1920

TCCACAAGAC TGCCTGTTTT TTAG 1944

Domain Profile

S: 1     velaekiGsGrfGtvyrgkwhGdvavkllnveelseeqleaFkkevaaykktRhenivLF  60

         v+l+++iGsG+fGtvy+gkwhGdvavk+l+v ++++eq++aF++eva+++ktRh+ni+LF

Q: 349   VMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLF  408

         57899*******************************************************

S: 61    mGyvskpelaivtslckgssLykllheqetkldlaklidiarqiaqgmdYLhakkilhkD  120

         mGy++k++laivt++c+gssLyk+lh+qetk+++ +lidiarq+aqgmdYLhak+i+h+D

Q: 409   MGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRD  468

         ************************************************************

S: 121   lksknilleeglkvvitDFgllsvkrllsgrrekqleipkgwilylapeviralsidleq  180

         +ks+ni+l+egl+v+i+DFgl++vk+++sg  ++q+e+p+g+il++apevir+      q

Q: 469   MKSNNIFLHEGLTVKIGDFGLATVKSRWSG--SQQVEQPTGSILWMAPEVIRM------Q  520

         ******************************..*********************......*

S: 181   delpfskesDvyafGivlyellarelpyke.esadqilfavgrG.lkpdlskvksklpke  238

         d++pfs++sDvy++Givlyel+++elpy++ +++dqi+f+vgrG ++pdlsk+++++pk+

Q: 521   DSNPFSFQSDVYSYGIVLYELMTGELPYSHiNNRDQIIFMVGRGyASPDLSKLYKNCPKA  580

         ************************************************************

S: 239   lkellleClkfekeeRPlftqilkkl  264

         +k+l+++Clk+ +eeRPlf+qil+++

Q: 581   MKRLVADCLKKVREERPLFPQILSSI  606

         **********************9875

Domain Sequence
(FASTA)

VMLSTRIGSG SFGTVYKGKW HGDVAVKILK VVDPTPEQFQ AFRNEVAVLR KTRHVNILLF 60

MGYMTKDNLA IVTQWCEGSS LYKHLHVQET KFQMFQLIDI ARQTAQGMDY LHAKNIIHRD 120

MKSNNIFLHE GLTVKIGDFG LATVKSRWSG SQQVEQPTGS ILWMAPEVIR MQDSNPFSFQ 180

SDVYSYGIVL YELMTGELPY SHINNRDQII FMVGRGYASP DLSKLYKNCP KAMKRLVADC 240

LKKVREERPL FPQILSSI 258

Keyword

ATP-binding; Complete proteome; Kinase; Metal-binding; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase; Zinc.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Macropus eugenii"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005622	; C:intracellular
GO:0031143	; C:pseudopodium
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0005057	; F:receptor signaling protein activity
GO:0006915	; P:apoptotic process
GO:0007010	; P:cytoskeleton organization
GO:0035556	; P:intracellular signal transduction
GO:0043066	; P:negative regulation of apoptotic process
GO:0008285	; P:negative regulation of cell proliferation
GO:0031333	; P:negative regulation of protein complex assembly
GO:0048011	; P:nerve growth factor receptor signaling pathway
GO:0033138	; P:positive regulation of peptidyl-serine phosphorylation

KEGG

mgp:100541264

;

InterPros

IPR020454	; DAG/PE-bd.
IPR011009	; Kinase-like_dom.
IPR002219	; Prot_Kinase_C-like_PE/DAG-bd.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR003116	; Raf-like_ras-bd.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00130	; C1_1; 1.
PF07714	; Pkinase_Tyr; 1.
PF02196	; RBD; 1.

SMARTs

SM00109	; C1; 1.
SM00455	; RBD; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS50898	; RBD; 1.
PS00479	; ZF_DAG_PE_1; 1.
PS50081	; ZF_DAG_PE_2; 1.

Prints

PR00008

; DAGPEDOMAIN.

Created Date

20-Feb-2013