EKS-MAM-00301

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MAM-00301

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/EGFR	498.4	6.7E-150	667	924	258

Status

Reviewed

Ensembl Protein

ENSMMUP00000029470

UniProt Accession

P55245;

Protein Name

Epidermal growth factor receptor

Protein Synonyms/Alias

Gene Name

EGFR

Gene Synonyms/Alias

EGFR;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMMUG00000022394	ENSMMUP00000029471	ENSMMUT00000031497
ENSMMUG00000022394	ENSMMUP00000029470	ENSMMUT00000031496
ENSMMUG00000022394	ENSMMUP00000029469	ENSMMUT00000031495

Organism

Macaca mulatta

Functional Description

Receptor tyrosine kinase binding ligands of the EGFfamily and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.

Protein Length

1078

Protein Sequence
(FASTA)

MRPSGTAGAA LLALLAALCP ASRALEEKKV CQGTSNKLTQ LGTFEDHFLS LQRMFNNCEV 60

VLGNLEITYV QRNYDLSFLK TIQEVAGYVL IALNTVERIP LENLQIIRGN MYYENSYALA 120

VLSNYDANKT GLKELPMRNL QGQKCDPSCP NGSCWGAGEE NCQKLTKIIC AQQCSGRCRG 180

KSPSDCCHNQ CAAGCTGPRE SDCLVCRKFR DEATCKDTCP PLMLYNPTTY QMDVNPEGKY 240

SFGATCVKKC PRNYVVTDHG SCVRACGADS YEMEEDGVRK CKKCEGPCRK VCNGIGIGEF 300

KDTLSINATN IKHFKNCTSI SGDLHILPVA FRGDSFTHTP PLDPQELDIL KTVKEITGFL 360

LIQAWPENRT DLHAFENLEI IRGRTKQHGQ FSLAVVSLNI TSLGLRSLKE ISDGDVIISG 420

NKNLCYANTI NWKKLFGTSS QKTKIISNRG ENSCKATGQV CHALCSPEGC WGPEPRDCVS 480

CQNVSRGREC VDKCNVLEGE PREFVENSEC IQCHPECLPQ VMNITCTGRG PDNCIQCAHY 540

IDGPHCVKTC PAGVMGENNT LVWKYADAGH VCHLCHPNCT YGCTGPGLEG CARNGPKIPS 600

IATGMVGALL LLLVVALGIG LFMRRRHIVR KRTLRRLLQE RELVEPLTPS GEAPNQALLR 660

ILKETEFKKI KVLGSGAFGT VYKGLWIPEG EKVKIPVAIK ELREATSPKA NKEILDEAYV 720

MASVDNPHVC RLLGICLTST VQLITQLMPF GCLLDYVREH KDNIGSQYLL NWCVQIAKGM 780

NYLEDRRLVH RDLAARNVLV KTPQHVKITD FGLAKLLGAE EKEYHAEGGK VPIKWMALES 840

ILHRIYTHQS DVWSYGVTVW ELMTFGSKPY DGIPASEISS ILEKGERLPQ PPICTIDVYM 900

IMVKCWMIDA DSRPKFRELI IEFSKMARDP QRYLVIQGDE RMHLPSPTDS NFYRALMDEE 960

DMDDVVDADE YLIPQQGFFS SPSTSRTPLL SSLSATSNNS TVACIDRNGL QSCPIKEDSF 1020

LQRYSSDPTG ALTEDSIDDT FLPVPGEWLV WKHSCSSASL THSAAASLHH PVSKPGAP 1078

Nucleotide Sequence
(FASTA)

ATGCGACCCT CCGGGACGGC CGGGGCCGCG CTCCTGGCGC TGCTGGCTGC GCTCTGCCCA 60

GCGAGTCGGG CTCTGGAGGA AAAGAAAGTT TGCCAAGGCA CGAGTAACAA ACTCACGCAG 120

TTGGGCACTT TTGAAGATCA TTTTCTCAGC CTCCAGAGGA TGTTCAATAA CTGTGAGGTG 180

GTCCTTGGGA ATTTGGAAAT TACCTACGTG CAGAGGAATT ATGATCTTTC CTTCTTAAAG 240

ACCATCCAGG AGGTGGCTGG TTATGTCCTC ATCGCCCTCA ACACAGTGGA GCGGATTCCT 300

TTGGAAAACC TGCAGATCAT CAGAGGAAAC ATGTACTATG AAAATTCCTA TGCCTTAGCA 360

GTCTTATCTA ACTATGATGC AAATAAAACC GGACTGAAGG AGCTGCCCAT GAGAAACTTA 420

CAGGGCCAAA AGTGTGATCC AAGCTGTCCC AATGGGAGCT GCTGGGGTGC AGGAGAGGAG 480

AACTGCCAGA AACTGACCAA AATCATCTGT GCCCAGCAGT GCTCCGGGCG CTGCCGCGGC 540

AAGTCCCCCA GTGACTGCTG CCACAACCAG TGTGCCGCGG GCTGCACGGG CCCCCGGGAG 600

AGCGACTGCC TGGTCTGCCG CAAATTCCGA GACGAAGCCA CGTGCAAGGA CACCTGCCCC 660

CCACTCATGC TCTACAACCC CACCACATAC CAGATGGATG TGAACCCCGA GGGCAAATAC 720

AGCTTTGGTG CCACCTGCGT GAAGAAGTGT CCCCGTAATT ATGTGGTGAC AGATCACGGC 780

TCGTGCGTCC GAGCCTGCGG GGCCGACAGC TATGAGATGG AGGAAGACGG CGTCCGCAAG 840

TGTAAGAAGT GCGAAGGGCC TTGCCGCAAA GTGTGTAATG GAATAGGTAT TGGTGAATTT 900

AAAGACACAC TCTCCATAAA TGCTACAAAT ATTAAACACT TCAAAAACTG CACCTCCATC 960

AGTGGCGATC TCCACATCCT GCCGGTGGCA TTTAGGGGTG ACTCCTTCAC ACACACTCCG 1020

CCTCTGGATC CACAGGAACT GGATATTCTG AAAACCGTAA AGGAAATCAC AGGGTTTTTG 1080

CTGATTCAGG CTTGGCCTGA AAACAGGACG GACCTCCATG CTTTTGAGAA CCTAGAAATC 1140

ATACGTGGCA GGACCAAGCA ACACGGTCAG TTTTCTCTTG CGGTCGTCAG CCTGAACATA 1200

ACATCCTTGG GATTACGCTC CCTCAAGGAG ATAAGCGATG GAGATGTGAT AATTTCAGGA 1260

AACAAAAATT TGTGCTATGC AAATACAATA AACTGGAAAA AACTGTTTGG GACCTCCAGT 1320

CAGAAAACCA AAATTATAAG CAACAGAGGT GAAAACAGCT GCAAGGCCAC GGGCCAGGTC 1380

TGCCATGCCT TGTGCTCCCC CGAGGGCTGC TGGGGCCCGG AGCCCAGGGA CTGCGTCTCC 1440

TGCCAGAATG TCAGCCGAGG CAGAGAATGC GTGGACAAGT GCAACGTCCT GGAGGGCGAG 1500

CCAAGGGAGT TTGTGGAGAA CTCTGAGTGC ATACAGTGCC ACCCAGAATG CCTGCCCCAG 1560

GTCATGAACA TCACCTGCAC AGGACGGGGA CCAGACAACT GTATCCAGTG TGCCCACTAC 1620

ATTGACGGCC CCCACTGCGT CAAGACCTGC CCAGCAGGAG TCATGGGAGA AAACAACACC 1680

CTGGTCTGGA AGTACGCAGA CGCCGGCCAC GTGTGCCACC TGTGCCATCC AAACTGCACC 1740

TACGGATGCA CTGGGCCAGG TCTTGAAGGC TGTGCAAGGA ACGGGCCTAA GATCCCATCC 1800

ATCGCCACTG GGATGGTGGG GGCCCTCCTC TTGCTGCTGG TGGTGGCCCT GGGGATCGGC 1860

CTCTTCATGC GAAGGCGCCA CATCGTTCGG AAGCGCACAC TGCGGAGGCT GCTGCAGGAG 1920

AGGGAGCTTG TGGAGCCTCT TACGCCCAGT GGAGAAGCTC CCAACCAAGC TCTCTTGAGG 1980

ATCTTGAAGG AAACTGAATT CAAGAAGATC AAAGTGCTGG GCTCCGGTGC GTTCGGAACT 2040

GTGTATAAGG GACTCTGGAT CCCAGAAGGT GAGAAAGTTA AAATTCCCGT CGCTATCAAG 2100

GAATTAAGAG AAGCAACATC TCCGAAAGCC AACAAGGAAA TCCTCGATGA AGCCTACGTG 2160

ATGGCCAGCG TGGACAACCC CCATGTGTGC CGCCTGCTGG GCATCTGCCT CACCTCCACC 2220

GTGCAGCTCA TTACGCAGCT CATGCCCTTC GGCTGCCTCC TGGACTACGT CCGGGAACAC 2280

AAGGACAATA TCGGCTCCCA GTACCTGCTC AACTGGTGTG TGCAGATTGC AAAGGGCATG 2340

AACTACTTGG AGGACCGGCG CTTGGTGCAC CGCGACCTGG CAGCCAGGAA CGTACTGGTG 2400

AAAACGCCAC AGCATGTCAA GATCACGGAT TTTGGGCTGG CCAAACTGCT GGGTGCAGAA 2460

GAGAAAGAAT ACCATGCAGA AGGAGGCAAA GTGCCTATCA AGTGGATGGC ATTGGAATCA 2520

ATTTTACACC GAATTTATAC CCACCAGAGT GATGTCTGGA GCTACGGGGT GACCGTTTGG 2580

GAGTTGATGA CCTTTGGATC CAAGCCATAT GACGGAATCC CTGCCAGCGA GATCTCCTCC 2640

ATCTTGGAGA AAGGAGAACG CCTCCCCCAG CCACCCATAT GTACCATCGA TGTCTACATG 2700

ATCATGGTCA AGTGCTGGAT GATAGACGCA GATAGTCGCC CAAAGTTCCG TGAGTTGATC 2760

ATTGAATTCT CCAAAATGGC CCGAGACCCC CAGCGCTACC TTGTTATTCA GGGGGATGAA 2820

AGAATGCATT TGCCAAGCCC TACAGACTCC AACTTCTACC GTGCCCTGAT GGATGAAGAA 2880

GACATGGACG ACGTGGTGGA TGCCGACGAG TACCTCATCC CACAGCAAGG CTTCTTCAGC 2940

AGCCCCTCCA CGTCACGGAC TCCCCTCCTG AGCTCTCTGA GTGCAACTAG CAACAATTCC 3000

ACTGTGGCTT GCATTGATAG AAATGGGCTG CAAAGCTGTC CCATCAAGGA AGACAGCTTC 3060

TTACAGCGAT ACAGCTCAGA CCCCACAGGC GCCTTGACTG AGGACAGCAT AGACGACACC 3120

TTCCTCCCAG TGCCTGGTGA GTGGCTTGTC TGGAAACATT CCTGCTCCTC AGCCTCCTTG 3180

ACCCACTCAG CAGCAGCCAG TCTCCACCAC CCAGTGTCCA AGCCAGGTGC TCCC 3234

Domain Profile

S: 1     lkklkvlGsGafGtvykGiwipegenvkipvaikvleektgkkankelldealvlasldh  60

         +kk+kvlGsGafGtvykG+wipege+vkipvaik+l+e+t++kanke+ldea+v+as+d+

Q: 667   FKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDN  726

         59**********************************************************

S: 61    anlvrllglclsstlqlvtqllplGclldyvrehkdklGsqellnwcvqiakGmsyleek  120

         ++++rllg+cl+st+ql+tql+p+Gclldyvrehkd++Gsq+llnwcvqiakGm+yle++

Q: 727   PHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDR  786

         ************************************************************

S: 121   rlvhrdlaarnvlvkspnhvkitdfGlaklleadekelkaeggkvpikwlalesilkrky  180

         rlvhrdlaarnvlvk+p+hvkitdfGlakll a+eke++aeggkvpikw+alesil+r+y

Q: 787   RLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIY  846

         ************************************************************

S: 181   thqsdvwsyGvtvwelltfGakpydgipakeipdllekGerlaqppictidvymvlvkcw  240

         thqsdvwsyGvtvwel+tfG+kpydgipa+ei+++lekGerl+qppictidvym++vkcw

Q: 847   THQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCW  906

         ************************************************************

S: 241   midaesrpkfkelaeefs  258

         mida+srpkf+el+ efs

Q: 907   MIDADSRPKFRELIIEFS  924

         ****************96

Domain Sequence
(FASTA)

FKKIKVLGSG AFGTVYKGLW IPEGEKVKIP VAIKELREAT SPKANKEILD EAYVMASVDN 60

PHVCRLLGIC LTSTVQLITQ LMPFGCLLDY VREHKDNIGS QYLLNWCVQI AKGMNYLEDR 120

RLVHRDLAAR NVLVKTPQHV KITDFGLAKL LGAEEKEYHA EGGKVPIKWM ALESILHRIY 180

THQSDVWSYG VTVWELMTFG SKPYDGIPAS EISSILEKGE RLPQPPICTI DVYMIMVKCW 240

MIDADSRPKF RELIIEFS 258

Keyword

ATP-binding; Cell membrane; Complete proteome; Endoplasmic reticulum; Endosome; Golgi apparatus; Isopeptide bond; Kinase; Membrane; Nucleotide-binding; Nucleus; Receptor; Reference proteome; Transferase; Tyrosine-protein kinase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Loxodonta africana"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0016323	; C:basolateral plasma membrane
GO:0030139	; C:endocytic vesicle
GO:0005789	; C:endoplasmic reticulum membrane
GO:0005768	; C:endosome
GO:0010008	; C:endosome membrane
GO:0000139	; C:Golgi membrane
GO:0045121	; C:membrane raft
GO:0031965	; C:nuclear membrane
GO:0048471	; C:perinuclear region of cytoplasm
GO:0005524	; F:ATP binding
GO:0005006	; F:epidermal growth factor-activated receptor activity
GO:0030235	; F:nitric-oxide synthase regulator activity
GO:0004713	; F:protein tyrosine kinase activity
GO:0000902	; P:cell morphogenesis
GO:0008283	; P:cell proliferation
GO:0016337	; P:cell-cell adhesion
GO:0021795	; P:cerebral cortex cell migration
GO:0048546	; P:digestive tract morphogenesis
GO:0001892	; P:embryonic placenta development
GO:0001942	; P:hair follicle development
GO:0060571	; P:morphogenesis of an epithelial fold
GO:0043066	; P:negative regulation of apoptotic process
GO:0042177	; P:negative regulation of protein catabolic process
GO:0035413	; P:positive regulation of catenin import into nucleus
GO:0030335	; P:positive regulation of cell migration
GO:0031659	; P:positive regulation of cyclin-dependent protein kinase activity involved in G1/S
GO:0045739	; P:positive regulation of DNA repair
GO:0045740	; P:positive regulation of DNA replication
GO:0050679	; P:positive regulation of epithelial cell proliferation
GO:0048146	; P:positive regulation of fibroblast proliferation
GO:0043406	; P:positive regulation of MAP kinase activity
GO:0045429	; P:positive regulation of nitric oxide biosynthetic process
GO:0051897	; P:positive regulation of protein kinase B signaling cascade
GO:0046777	; P:protein autophosphorylation
GO:0050999	; P:regulation of nitric-oxide synthase activity
GO:0050730	; P:regulation of peptidyl-tyrosine phosphorylation
GO:0070141	; P:response to UV-A
GO:0007435	; P:salivary gland morphogenesis

KEGG

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.

Pfam

PF07714

; Pkinase_Tyr; 1.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; PARTIAL.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; PARTIAL.

Prints

PR00109

; TYRKINASE.

Created Date

20-Feb-2013