EKS-MUM-00044

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00044

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CK1/VRK	378.2	4.7E-113	145	411	267

Status

Reviewed

Ensembl Protein

ENSMUSP00000002275

UniProt Accession

Q8K3G5; Q921W6;

Protein Name

Inactive serine/threonine-protein kinase VRK3

Protein Synonyms/Alias

Serine/threonine-protein pseudokinase VRK3; Vaccinia-related kinase 3;

Gene Name

Vrk3

Gene Synonyms/Alias

Vrk3;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000002205	ENSMUSP00000132748	ENSMUST00000171821
ENSMUSG00000002205	ENSMUSP00000130331	ENSMUST00000147952
ENSMUSG00000002205	ENSMUSP00000002275	ENSMUST00000002275
ENSMUSG00000002205	ENSMUSP00000119073	ENSMUST00000144515
ENSMUSG00000002205	ENSMUSP00000131704	ENSMUST00000165957
ENSMUSG00000002205	ENSMUSP00000122114	ENSMUST00000125705

Organism

Mus musculus

Functional Description

Inactive kinase that suppresses ERK activity bypromoting phosphatase activity of DUSP3 which specifically dephosphorylates and inactivates ERK in the nucleus.

Protein Length

453

Protein Sequence
(FASTA)

MISFCPVCGK SVKVSFKFCP YCGKALPVEE DGGTQSAVTP HVSSVPGSRR DLNSSFETSP 60

KKVKCSHTVT SLPLSRHSDC DSSGSDNTLT SPDRATGTRS RPLTPKGSPL SNRQSPQTLK 120

RTRVTTSLQA LATGTELTDQ NGKHWTLGAL QIRDDQGILY EAEPTSAVPS ESRTQKWRFS 180

LKLDSKDGRL FNEQNFFQRV AKPLQVNKWK KQFLLPLLAI PTCIGFGIHQ DKYRFLVFPS 240

LGRSLQSALD DNPKHVVSER CVLQVACRLL DALEYLHENE YVHGNLTAEN VFVNPEDLSQ 300

VTLVGYGFTY RYCPGGKHVA YKEGSRSPHD GDLEFISMDL HKGCGPSRRS DLQTLGYCML 360

KWLYGSLPWT NCLPNTEKIT RQKQKYLDSP ERLVGLCGRW NKASETLREY LKVVMALNYE 420

EKPPYATLRN SLEALLQDMR VSPYDPLDLQ MVP 453

Nucleotide Sequence
(FASTA)

ATGATCTCCT TCTGTCCAGT CTGTGGCAAA AGTGTCAAGG TATCATTCAA GTTCTGCCCT 60

TACTGTGGCA AGGCCCTGCC TGTAGAGGAG GATGGAGGCA CCCAGTCTGC CGTGACGCCT 120

CATGTGTCAT CCGTTCCAGG CTCCAGGAGA GACCTGAACT CCAGTTTTGA AACCTCTCCC 180

AAGAAAGTGA AATGCTCACA CACTGTCACT TCTCTCCCAC TCTCCCGCCA CTCTGACTGT 240

GACAGTTCTG GGTCAGACAA CACCTTGACT TCTCCTGACA GGGCCACAGG CACCCGGAGC 300

AGACCCCTGA CCCCCAAAGG CAGCCCACTT TCCAACAGGC AGAGCCCTCA GACTCTGAAG 360

CGGACCCGGG TGACCACCTC ACTCCAGGCT CTAGCCACAG GAACAGAGCT GACAGACCAG 420

AATGGGAAAC ACTGGACCCT GGGGGCCCTG CAGATCCGGG ATGACCAAGG CATTCTCTAT 480

GAAGCTGAGC CTACTTCTGC CGTCCCCTCT GAATCAAGGA CCCAGAAGTG GAGATTCTCA 540

CTCAAACTGG ACTCCAAGGA TGGGCGCCTT TTCAATGAGC AGAACTTCTT TCAGAGAGTA 600

GCCAAGCCTC TGCAAGTGAA CAAATGGAAG AAGCAGTTCT TGCTCCCGCT CCTGGCCATC 660

CCCACCTGTA TCGGCTTTGG CATTCACCAG GACAAGTACA GGTTCCTAGT ATTCCCCAGC 720

CTGGGGAGGA GCCTTCAGTC AGCCCTGGAT GACAACCCAA AGCATGTGGT ATCAGAGAGA 780

TGTGTGCTTC AGGTGGCCTG CAGGCTGCTG GATGCTCTGG AGTATCTCCA TGAAAATGAG 840

TATGTTCACG GGAACCTGAC AGCTGAGAAT GTCTTTGTGA ATCCAGAGGA TCTGAGCCAG 900

GTGACCCTGG TGGGCTATGG CTTCACCTAC CGATACTGCC CAGGTGGCAA ACACGTGGCC 960

TACAAAGAAG GCAGCAGGAG TCCACACGAT GGGGACTTGG AGTTCATTAG CATGGACCTG 1020

CACAAGGGAT GCGGACCCTC CCGCCGCAGC GATCTCCAGA CCTTGGGCTA CTGTATGCTC 1080

AAGTGGCTTT ATGGGTCCCT GCCATGGACA AATTGCCTTC CCAACACCGA AAAGATAACT 1140

AGGCAGAAGC AGAAGTATCT GGACAGCCCC GAGCGCCTCG TGGGACTGTG TGGCCGCTGG 1200

AACAAGGCCT CAGAGACCCT GCGGGAGTAC CTGAAGGTGG TGATGGCCCT CAATTATGAG 1260

GAGAAGCCAC CCTATGCCAC GCTGAGGAAC AGCCTAGAAG CTCTGCTGCA GGATATGCGG 1320

GTGTCACCCT ATGACCCTCT GGACCTCCAG ATGGTGCCTT AG 1362

Domain Profile

S: 1     wklgkkigkggfGeiyla.....ltckkeekdaklvvkvepkenGpLfvelkfyqraakk  55

         w+lg  + ++++G++y+a     +++++  ++ ++++k++ k+ G+Lf+e++f+qr+ak+

Q: 145   WTLGALQIRDDQGILYEAeptsaVPSESRTQKWRFSLKLDSKD-GRLFNEQNFFQRVAKP  203

         999***************9987778888899999******999.****************

S: 56    eqikkwkkkkklkllgiPtliasGlhefegekyRflvlprlgrdLqklleqngkr.lsek  114

          q++kwkk++ l+ll+iPt+i++G+h+   +kyRflv+p+lgr+Lq++l++n+k+ +se+

Q: 204   LQVNKWKKQFLLPLLAIPTCIGFGIHQ---DKYRFLVFPSLGRSLQSALDDNPKHvVSER  260

         ***************************...*************************99***

S: 115   tvlqlavrlldvleylHeneYvHgdikAanllleeeeasqvyLvdyGlayrycpegkhke  174

         +vlq+a+rlld+leylHeneYvHg+++A+n+++++e+ sqv+Lv+yG++yrycp+gkh++

Q: 261   CVLQVACRLLDALEYLHENEYVHGNLTAENVFVNPEDLSQVTLVGYGFTYRYCPGGKHVA  320

         ************************************************************

S: 175   ykedkrraHdGtleftslDaHkGvapsrRsDleiLgYcllkWltgkLPWeallkdpekvq  234

         yke++r++HdG+lef+s+D+HkG++psrRsDl++LgYc+lkWl+g+LPW+++l+++ek++

Q: 321   YKEGSRSPHDGDLEFISMDLHKGCGPSRRSDLQTLGYCMLKWLYGSLPWTNCLPNTEKIT  380

         ************************************************************

S: 235   kakekfldelpellkkllkkkssseelakyl  265

         ++k+k+ld++++l+  + +++++se+l++yl

Q: 381   RQKQKYLDSPERLVGLCGRWNKASETLREYL  411

         ******************************7

Domain Sequence
(FASTA)

WTLGALQIRD DQGILYEAEP TSAVPSESRT QKWRFSLKLD SKDGRLFNEQ NFFQRVAKPL 60

QVNKWKKQFL LPLLAIPTCI GFGIHQDKYR FLVFPSLGRS LQSALDDNPK HVVSERCVLQ 120

VACRLLDALE YLHENEYVHG NLTAENVFVN PEDLSQVTLV GYGFTYRYCP GGKHVAYKEG 180

SRSPHDGDLE FISMDLHKGC GPSRRSDLQT LGYCMLKWLY GSLPWTNCLP NTEKITRQKQ 240

KYLDSPERLV GLCGRWNKAS ETLREYL 267

Keyword

Complete proteome; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0004672	; F:protein kinase activity
GO:0070373	; P:negative regulation of ERK1 and ERK2 cascade
GO:0032516	; P:positive regulation of phosphoprotein phosphatase activity

KEGG

mmu:101568

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.

Pfam

PF00069

; Pkinase; 1.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; FALSE_NEG.

Prints

Created Date

20-Feb-2013