EKS-MUM-00408

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00408

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TKL/MLK	347.5	1.1E-103	120	377	258

Status

Reviewed

Ensembl Protein

ENSMUSP00000004156

UniProt Accession

Q80XI6; Q8K0M8; Q9JJ15;

Protein Name

Mitogen-activated protein kinase kinase kinase 11

Protein Synonyms/Alias

Mixed lineage kinase 3;

Gene Name

Map3k11

Gene Synonyms/Alias

Map3k11; Mlk3;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000004054	ENSMUSP00000004156	ENSMUST00000004156

Organism

Mus musculus

Functional Description

Activates the JUN N-terminal pathway. Required forserum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle (By similarity).

Protein Length

850

Protein Sequence
(FASTA)

MEPLKNLFLK SPLGSWNGSG SGGGGGTGGV RPEGSPKATA AYANPVWTAL FDYEPNGQDE 60

LALRKGDRVE VLSRDAAISG DEGWWAGQVG GQVGIFPSNY VSRGGGPPPC EVASFQELRL 120

EEVIGIGGFG KVYRGSWRGE LVAVKAARQD PDEDISVTAE SVRQEARLFA MLAHPNIIAL 180

KAVCLEEPNL CLVMEYAAGG PLSRALAGRR VPPHVLVNWA VQIARGMHYL HCEALVPVIH 240

RDLKSNNILL LQPIEGDDME HKTLKITDFG LAREWHKTTQ MSAAGTYAWM APEVIKASTF 300

SKGSDVWSFG VLLWELLTGE VPYRGIDCLA VAYGVAVNKL TLPIPSTCPE PFAQLMADCW 360

AQDPHRRPDF ASILQQLEAL EAQVLREMPR DSFHSMQEGW KREIQGLFDE LRAKEKELLS 420

REEELTRAAR EQRSQAEQLR RREHLLAQWE LEVFERELTL LLQQVDRERP HVRRRRGTFK 480

RSKLRARDGG ERISMPLDFK HRITVQASPG LDRRRNVFEV GAGDSPTFPR FRAIQLEPTE 540

SGQTWGRQSP RRLEDSSNGE RRACWAWGPS SPKPGEAQNG RRRSRMDEAT WYLDSDDSSP 600

LGSPSTPPAL NGNPPRPSPE PEEPRRAGPT ERGNSSGTPK LIQRALLRGT ALLASLGLGR 660

DLQPPGGLSR ERGESPTAPP PAQMPSPCPP ELPSTPLIRL SQTTPDAHSS PTPGPLLLDL 720

GVPSGQPSAK SPRREETRGR TVSPPPGISR SAPGTPGTPR SPPLGLISRP RPSPLRSRID 780

PWSFVSAGPR PSPLPSPQPA PRRAPWTLFP DSDPFWDSPP ANPFRGGSQD CRTQTKDMGA 840

QAPWAPEAGP 850

Nucleotide Sequence
(FASTA)

ATGGAGCCCT TGAAGAACCT CTTCCTCAAG AGCCCGCTGG GGTCATGGAA CGGCAGTGGC 60

AGTGGGGGCG GCGGGGGTAC CGGTGGAGTC CGGCCGGAGG GGTCTCCGAA GGCAACAGCA 120

GCTTATGCCA ATCCTGTCTG GACAGCCCTG TTCGACTATG AGCCCAATGG GCAGGACGAA 180

CTAGCCCTGA GGAAGGGCGA CCGTGTGGAG GTGCTGTCCA GGGATGCAGC CATCTCAGGA 240

GATGAGGGCT GGTGGGCGGG CCAGGTGGGT GGCCAGGTGG GCATTTTTCC ATCCAACTAT 300

GTATCTCGGG GTGGAGGCCC ACCCCCCTGT GAGGTGGCCA GCTTCCAGGA GCTGCGACTG 360

GAGGAGGTGA TCGGCATCGG TGGCTTCGGC AAGGTCTACC GTGGCAGCTG GCGAGGGGAG 420

CTGGTGGCTG TGAAGGCAGC CCGACAGGAC CCTGATGAGG ACATCAGTGT AACAGCTGAG 480

AGTGTTCGAC AGGAGGCCCG GCTTTTTGCC ATGCTGGCCC ATCCCAACAT CATTGCCCTC 540

AAAGCTGTGT GCCTGGAGGA GCCAAACCTA TGCCTGGTGA TGGAGTATGC AGCTGGTGGG 600

CCTCTTAGCC GGGCCCTGGC TGGTCGGCGT GTGCCCCCCC ATGTGCTGGT CAACTGGGCT 660

GTGCAGATTG CCCGAGGAAT GCACTACCTG CACTGTGAGG CTCTGGTGCC TGTGATACAC 720

CGAGACCTGA AGTCCAACAA CATTCTGTTG CTGCAGCCCA TCGAGGGTGA CGACATGGAA 780

CACAAGACCC TAAAGATTAC TGACTTCGGC CTCGCCCGAG AGTGGCACAA AACCACCCAG 840

ATGAGTGCTG CGGGCACCTA CGCTTGGATG GCTCCCGAGG TTATCAAGGC CTCCACCTTC 900

TCCAAGGGCA GCGACGTCTG GAGCTTTGGA GTGCTGCTGT GGGAACTGCT GACTGGGGAG 960

GTGCCCTACC GTGGCATCGA CTGTCTTGCC GTAGCCTATG GTGTGGCTGT AAACAAGTTA 1020

ACGTTACCCA TCCCATCTAC CTGCCCTGAG CCCTTTGCAC AACTCATGGC TGACTGCTGG 1080

GCACAGGACC CCCACCGCAG GCCCGACTTC GCCTCCATCC TGCAGCAGTT GGAAGCTCTA 1140

GAAGCGCAGG TGCTGCGGGA GATGCCACGG GACTCCTTCC ATTCCATGCA GGAAGGCTGG 1200

AAACGCGAGA TCCAGGGCCT GTTTGATGAG CTGCGGGCCA AGGAAAAGGA ACTACTGAGC 1260

CGGGAGGAGG AACTGACGCG CGCTGCACGT GAGCAGCGGT CCCAGGCGGA GCAGCTGCGA 1320

CGGCGCGAGC ACCTGCTGGC GCAGTGGGAG CTGGAGGTGT TCGAGCGCGA GCTGACGCTG 1380

CTACTGCAGC AGGTGGACCG AGAACGGCCG CACGTGCGCC GCCGCCGGGG CACCTTCAAG 1440

CGGAGCAAAC TCCGAGCAAG GGACGGCGGA GAACGCATCA GCATGCCCCT GGATTTCAAA 1500

CACCGCATCA CAGTGCAAGC CTCTCCTGGC CTGGATCGGA GGAGAAACGT GTTCGAGGTT 1560

GGGGCTGGGG ACTCGCCCAC CTTCCCTAGA TTCCGAGCCA TCCAGTTGGA ACCTACAGAA 1620

TCAGGCCAGA CTTGGGGTCG CCAGTCCCCC AGACGTCTTG AAGATTCAAG CAATGGAGAG 1680

AGGCGTGCTT GCTGGGCCTG GGGTCCCAGC TCCCCCAAGC CTGGGGAGGC CCAGAACGGG 1740

AGGAGACGGT CCCGCATGGA TGAAGCCACG TGGTACCTAG ATTCAGACGA CTCGTCCCCA 1800

TTAGGGTCCC CTAGTACTCC CCCGGCACTC AACGGTAATC CTCCAAGGCC TAGCCCCGAG 1860

CCAGAGGAGC CACGGAGGGC CGGGCCCACG GAGCGAGGCA ATAGCTCTGG GACGCCTAAA 1920

CTGATCCAAC GTGCATTGCT ACGGGGGACC GCTCTGCTGG CTTCCCTAGG CCTTGGCCGA 1980

GATCTGCAAC CTCCAGGAGG CCTGAGCCGT GAACGCGGGG AGTCCCCGAC AGCACCACCC 2040

CCTGCACAGA TGCCCTCACC CTGCCCACCT GAGCTGCCCT CCACCCCGCT CATCCGCCTC 2100

TCACAAACAA CACCTGATGC CCATAGTTCA CCCACTCCTG GACCCTTATT GCTAGACCTA 2160

GGTGTTCCCT CTGGCCAGCC ATCAGCCAAG AGCCCTCGAC GGGAAGAGAC ACGTGGGAGA 2220

ACCGTCTCAC CCCCACCAGG AATATCACGC TCTGCTCCTG GCACACCTGG AACCCCTCGC 2280

TCACCACCGT TGGGCCTAAT CAGCCGACCT CGGCCCTCAC CACTCCGTAG CCGTATTGAC 2340

CCATGGAGCT TCGTGTCAGC TGGGCCACGG CCTTCACCCC TGCCCTCTCC ACAGCCTGCA 2400

CCCCGACGGG CACCGTGGAC CTTATTCCCA GACTCAGATC CCTTCTGGGA CTCCCCACCT 2460

GCCAACCCCT TCCGGGGAGG CTCCCAGGAC TGCAGGACGC AGACCAAAGA CATGGGTGCC 2520

CAGGCCCCAT GGGCACCAGA AGCAGGGCCC TGA 2553

Domain Profile

S: 3     leeligsgafgkvykakwrkkevavKklreeaee..kekleklereakllsklshpniie  60

         lee+ig g fgkvy+++wr++ vavK++r++  e  + ++e++++ea+l+++l+hpnii+

Q: 120   LEEVIGIGGFGKVYRGSWRGELVAVKAARQDPDEdiSVTAESVRQEARLFAMLAHPNIIA  179

         789****************************99988999*********************

S: 61    llGvcleepnlllvmeyveggsLsnvlhekskreidlevlvswalqiArGmaYLhseapk  120

         l++vcleepnl+lvmey++gg Ls++l++   r+++++vlv+wa+qiArGm+YLh ea +

Q: 180   LKAVCLEEPNLCLVMEYAAGGPLSRALAG---RRVPPHVLVNWAVQIARGMHYLHCEALV  236

         *****************************...9***************************

S: 121   kiihrdLksknvLl.......seekktlkicdFGtarelskstkmsaagtlawmaPEvlk  173

         ++ihrdLks+n+Ll       ++e+ktlki+dFG are++k+t+msaagt+awmaPEv+k

Q: 237   PVIHRDLKSNNILLlqpiegdDMEHKTLKITDFGLAREWHKTTQMSAAGTYAWMAPEVIK  296

         *********************9**************************************

S: 174   eekvseksDvwSygvvLWelltrevPykdlselaiaykvalkglrlpipsscpeklkkLm  233

         ++++s+ sDvwS+gv+LWellt+evPy++++ la+ay+va+++l+lpips+cpe++++Lm

Q: 297   ASTFSKGSDVWSFGVLLWELLTGEVPYRGIDCLAVAYGVAVNKLTLPIPSTCPEPFAQLM  356

         ************************************************************

S: 234   kecwnedpekRpsfseivksl  254

          +cw++dp++Rp+f++i+++l

Q: 357   ADCWAQDPHRRPDFASILQQL  377

         *****************9986

Domain Sequence
(FASTA)

LEEVIGIGGF GKVYRGSWRG ELVAVKAARQ DPDEDISVTA ESVRQEARLF AMLAHPNIIA 60

LKAVCLEEPN LCLVMEYAAG GPLSRALAGR RVPPHVLVNW AVQIARGMHY LHCEALVPVI 120

HRDLKSNNIL LLQPIEGDDM EHKTLKITDF GLAREWHKTT QMSAAGTYAW MAPEVIKAST 180

FSKGSDVWSF GVLLWELLTG EVPYRGIDCL AVAYGVAVNK LTLPIPSTCP EPFAQLMADC 240

WAQDPHRRPD FASILQQL 258

Keyword

ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; SH3 domain; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Dipodomys ordii"; ?>Equus caballus"; ?>Felis catus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005813	; C:centrosome
GO:0005874	; C:microtubule
GO:0005524	; F:ATP binding
GO:0004706	; F:JUN kinase kinase kinase activity
GO:0004713	; F:protein tyrosine kinase activity
GO:0007257	; P:activation of JUN kinase activity
GO:0008219	; P:cell death
GO:0008283	; P:cell proliferation
GO:0000080	; P:G1 phase of mitotic cell cycle
GO:0007017	; P:microtubule-based process
GO:0046330	; P:positive regulation of JNK cascade
GO:0043525	; P:positive regulation of neuron apoptotic process
GO:0046777	; P:protein autophosphorylation
GO:0046328	; P:regulation of JNK cascade

KEGG

mmu:26403

;

InterPros

IPR011009	; Kinase-like_dom.
IPR016231	; MAPKKK9/10/11.
IPR015785	; MAPKKK9/10/11-like.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008271	; Ser/Thr_kinase_AS.
IPR011511	; SH3_2.
IPR001452	; SH3_domain.
IPR020635	; Tyr_kinase_cat_dom.

Pfam

PF07714	; Pkinase_Tyr; 1.
PF07653	; SH3_2; 1.

SMARTs

SM00326	; SH3; 1.
SM00219	; TyrKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS50002	; SH3; 1.

Prints

PR00452	; SH3DOMAIN.
PR00109	; TYRKINASE.

Created Date

20-Feb-2013