EKS-MUM-00413

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00413

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TKL/MLK	319.3	4.5E-95	17	260	244

Status

Reviewed

Ensembl Protein

ENSMUSP00000118983

UniProt Accession

Q9ESL4; Q3V1X8; Q8BR73; Q9ESL3;

Protein Name

Mitogen-activated protein kinase kinase kinase MLT

Protein Synonyms/Alias

Leucine zipper- and sterile alpha motif kinase ZAK; MLK-like mitogen-activated protein triple kinase; Mixed lineage kinase-related kinase; MLK-related kinase; MRK; Sterile alpha motif- and leucine zipper-containing kinase AZK;

Gene Name

Mltk

Gene Synonyms/Alias

Mltk;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000004085	ENSMUSP00000118983	ENSMUST00000135469
ENSMUSG00000004085	ENSMUSP00000119340	ENSMUST00000144111
ENSMUSG00000004085	ENSMUSP00000088334	ENSMUST00000090824

Organism

Mus musculus

Functional Description

Stress-activated component of a protein kinase signaltransduction cascade. Regulates the JNK and p38 pathways. Pro- apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2. Isoform 1, but not isoform 2, phosphorylates histone H3 at 'Ser-28'.

Protein Length

454

Protein Sequence
(FASTA)

MSSLGASFVQ IKFDDLQFFE NCGGGSFGSV YRAKWISQDK EVAVKKLLKI EKEAEILSVL 60

SHRNIIQFYG VILEPPNYGI VTEYASLGSL YDYINSNRSE EMDMEHIMTW ATDVAKGMHY 120

LHMEAPVKVI HRDLKSRNVV IAADGVLKIC DFGASRFHNH TTHMSLVGTF PWMAPEVIQS 180

LPVSETCDTY SYGVVLWEML TREVPFKGLE GLQVAWLVVE KNERLTIPSS CPRSFAELLH 240

QCWEADAKKR PSFKQIISIL ESMSNDTNLP DQCNSFLHNK AEWRCEIEAT LERLKKLERD 300

LSFKEQELKE RERRLKMWEQ KLTEQSNTPL LLPLSARMSE ESYFESKTEE SNSAEMSCQI 360

TAASNGEGHG MNPGLQAMML MGFGDVFSMN KAGAVLHSGM QINMQAKQNS SKTTCKRRGK 420

KVNMALGFSD FDLSEGDDDD HDGDDAENDV DNSE 454

Nucleotide Sequence
(FASTA)

ATGTCGTCTC TCGGAGCCTC CTTTGTGCAA ATTAAGTTCG ACGACTTGCA GTTTTTTGAA 60

AACTGCGGTG GAGGAAGTTT TGGGAGTGTG TATCGAGCCA AATGGATATC ACAGGACAAG 120

GAGGTGGCTG TAAAGAAGTT ACTCAAAATA GAGAAAGAGG CAGAAATCCT GAGCGTCCTC 180

AGTCACAGAA ACATCATCCA GTTTTATGGA GTGATTCTGG AACCTCCCAA CTATGGCATC 240

GTCACAGAAT ATGCTTCCCT GGGCTCCCTG TATGATTACA TTAACAGCAA CAGGAGTGAA 300

GAGATGGACA TGGAACACAT CATGACCTGG GCCACTGACG TAGCCAAAGG GATGCATTAC 360

TTACACATGG AAGCTCCTGT CAAGGTGATC CACAGAGACC TCAAGTCAAG AAACGTTGTT 420

ATCGCTGCTG ACGGAGTGCT GAAGATCTGT GACTTCGGTG CCTCGCGGTT CCATAACCAC 480

ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 540

CTCCCTGTGT CTGAAACCTG TGACACGTAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 600

ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 660

AAAAACGAGA GATTAACCAT TCCAAGCAGC TGCCCCAGAA GCTTTGCTGA ACTGCTACAC 720

CAGTGTTGGG AGGCTGATGC CAAGAAACGG CCATCGTTCA AGCAAATCAT TTCAATCCTG 780

GAGTCTATGT CAAATGACAC GAACCTTCCT GACCAGTGTA ACTCATTTTT ACACAACAAG 840

GCGGAGTGGA GGTGTGAAAT TGAGGCAACC CTTGAGCGAC TGAAGAAACT AGAGCGAGAT 900

CTCAGCTTTA AAGAGCAGGA GCTCAAAGAA CGGGAGAGAC GTCTCAAGAT GTGGGAGCAG 960

AAGCTGACGG AGCAATCCAA CACCCCGCTT CTCTTGCCTC TCTCTGCAAG AATGTCTGAG 1020

GAGTCTTACT TTGAATCTAA AACAGAGGAG TCAAACAGTG CAGAGATGTC ATGCCAGATC 1080

ACTGCAGCAA GTAACGGGGA GGGCCATGGC ATGAACCCAG GCCTGCAGGC CATGATGCTC 1140

ATGGGCTTTG GGGATGTCTT CTCAATGAAC AAAGCAGGAG CTGTGCTGCA TTCTGGGATG 1200

CAGATAAACA TGCAAGCCAA GCAGAATTCA TCCAAAACCA CATGTAAGAG GAGAGGGAAG 1260

AAAGTCAACA TGGCCCTGGG GTTCAGTGAC TTTGACCTGT CAGAAGGTGA CGATGATGAC 1320

CATGATGGTG ACGATGCTGA GAATGATGTG GATAATAGTG AATGA 1365

Domain Profile

S: 2     eleeligsgafgkvykakwrkke..vavKklreeaeekekleklereakllsklshpnii  59

         ++ e +g g+fg+vy+akw +++  vavKkl +          +e+ea++ls lsh+nii

Q: 17    QFFENCGGGSFGSVYRAKWISQDkeVAVKKLLK----------IEKEAEILSVLSHRNII  66

         67799**************665444****9987..........899**************

S: 60    ellGvcleepnlllvmeyveggsLsnvlhekskreidlevlvswalqiArGmaYLhseap  119

         +++Gv+le+pn+++v+ey++ gsL++++++++++e+d+e++++wa+++A+Gm+YLh eap

Q: 67    QFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMEHIMTWATDVAKGMHYLHMEAP  126

         ************************************************************

S: 120   kkiihrdLksknvLlseekktlkicdFGtarelskstkmsaagtlawmaPEvlkeekvse  179

         +k+ihrdLks+nv++ +++ +lkicdFG +r+++++t+ms  gt+ wmaPEv+++ +vse

Q: 127   VKVIHRDLKSRNVVI-AADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSE  185

         ***************.********************************************

S: 180   ksDvwSygvvLWelltrevPykdlselaiaykvalkglrlpipsscpeklkkLmkecwne  239

         ++D +SygvvLWe+ltrevP+k+l+ l++a+ v++k++rl+ipsscp+++++L+++cw++

Q: 186   TCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEA  245

         ************************************************************

S: 240   dpekRpsfseivksl  254

         d++kRpsf++i+++l

Q: 246   DAKKRPSFKQIISIL  260

         ***********9875

Domain Sequence
(FASTA)

QFFENCGGGS FGSVYRAKWI SQDKEVAVKK LLKIEKEAEI LSVLSHRNII QFYGVILEPP 60

NYGIVTEYAS LGSLYDYINS NRSEEMDMEH IMTWATDVAK GMHYLHMEAP VKVIHRDLKS 120

RNVVIAADGV LKICDFGASR FHNHTTHMSL VGTFPWMAPE VIQSLPVSET CDTYSYGVVL 180

WEMLTREVPF KGLEGLQVAW LVVEKNERLT IPSSCPRSFA ELLHQCWEAD AKKRPSFKQI 240

ISIL 244

Keyword

Alternative splicing; ATP-binding; Cell cycle; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0000287	; F:magnesium ion binding
GO:0004709	; F:MAP kinase kinase kinase activity
GO:0007257	; P:activation of JUN kinase activity
GO:0007050	; P:cell cycle arrest
GO:0000075	; P:cell cycle checkpoint
GO:0007010	; P:cytoskeleton organization
GO:0000077	; P:DNA damage checkpoint
GO:0043065	; P:positive regulation of apoptotic process
GO:0009314	; P:response to radiation

KEGG

mmu:65964

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR001660	; SAM.
IPR013761	; SAM/pointed.
IPR021129	; SAM_type1.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF07714	; Pkinase_Tyr; 1.
PF00536	; SAM_1; 1.

SMARTs

SM00454

; SAM; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS50105	; SAM_DOMAIN; 1.

Prints

PR00109

; TYRKINASE.

Created Date

20-Feb-2013