EKS-MUM-00224

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00224

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
AGC/PKN	555.5	3.8E-167	608	867	260

Status

Reviewed

Ensembl Protein

ENSMUSP00000133691

UniProt Accession

Q8BWW9; Q3TBR3; Q80WS2; Q8BJL7; Q8BL62;

Protein Name

Serine/threonine-protein kinase N2

Protein Synonyms/Alias

PKN gamma; Protein kinase C-like 2; Protein-kinase C-related kinase 2;

Gene Name

Pkn2

Gene Synonyms/Alias

Pkn2; Prk2, Prkcl2;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000004591	ENSMUSP00000134046	ENSMUST00000173913
ENSMUSG00000004591	ENSMUSP00000134559	ENSMUST00000174422
ENSMUSG00000004591	ENSMUSP00000133691	ENSMUST00000173830
ENSMUSG00000004591	ENSMUSP00000134041	ENSMUST00000174680
ENSMUSG00000004591	ENSMUSP00000039566	ENSMUST00000043812
ENSMUSG00000004591	ENSMUSP00000133419	ENSMUST00000173615

Organism

Mus musculus

Functional Description

PKC-related serine/threonine-protein kinase and Rho/Raceffector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c- fos serum response factor (SRF).

Protein Length

935

Protein Sequence
(FASTA)

MASNPDRGEI LLTELQGDSR TLPFSENVSA VQKLDFSDTM VQQKLDDIKD RIKREIRKEL 60

KIKEGAENLR KVTTDKKNLA YVDNILKKSN KKLEELHHKL QELNAHIVVS DPEDSTDCPR 120

TPDTPNSDSR SSTSNNRLMA LQKQLDIELK VKQGAENMIQ MYSNGSSKDR KLHGTAQQLL 180

QDSKTKIEVI RMQILQAVQT NELAFDNAKP VISPLELRME ELRHHFKIEF AVAEGAKNVM 240

KLLGSGKVTD RKALSEAQAR FNESSQKLDL LKYSLEQRLN ELPRNHPKSS VVIEELSLVA 300

SPTLSPRQSM LSTQNQYSTL SKPAALTGTL EVRLMGCQDI LENVPGRSKA TSVALPGWSP 360

SDNRSSFMSR TSKSKSGSSR NLLKTDDLSN DVCAVLKLDN TVVGQTSWKP ISNQSWDQKF 420

TLELDRVTFF NPVIERRPKL QRQKKIFSKQ QGKTFLRAPQ MNINIATWGR LVRRAIPTVN 480

HSGTFSPQTP VPATVPVVDA RIPDLAPPAS DSTVTKLDFD LEPEPPPAPP RASSLGETDE 540

SSELRVLDIP GQGSETVFNI ENDRNNLRPK SKSEYELSIP DSGRSCWGVG ELDDKRAQQR 600

FQFSLQDFRC CAVLGRGHFG KVLLAEYKHT NEMFAIKALK KGDIVARDEV DSLMCEKRIF 660

ETVNSVRHPF LVNLFACFQT KEHVCFVMEY AAGGDLMMHI HTDVFSEPRA VFYAACVVLG 720

LQYLHEHKIV YRDLKLDNLL LDTEGFVKIA DFGLCKEGMG YGDRTSTFCG TPEFLAPEVL 780

TETSYTRAVD WWGLGVLIYE MLVGESPFPG DDEEEVFDSI VNDEVRYPRF LSTEAISIMR 840

RLLRRNPERR LGAGEKDAED VKKHPFFRLT DWSALMDKKV KPPFVPTIRG REDVSNFDDE 900

FTSEAPILTP PREPRILLEE EQEMFHDFDY VADWC 935

Nucleotide Sequence
(FASTA)

ATGGCGTCCA ACCCCGACCG GGGGGAGATC CTCCTCACCG AGCTGCAGGG AGATTCCCGG 60

ACTCTTCCAT TTTCTGAGAA TGTGAGTGCT GTTCAGAAAT TAGACTTTTC AGATACAATG 120

GTGCAACAGA AATTGGACGA CATCAAGGAT CGAATAAAGA GAGAAATAAG GAAAGAACTG 180

AAAATCAAAG AAGGAGCTGA AAACCTGAGG AAAGTTACAA CAGATAAGAA AAATTTGGCT 240

TATGTAGACA ACATTTTGAA AAAATCAAAT AAAAAATTAG AAGAATTACA TCACAAGCTA 300

CAAGAATTAA ATGCACACAT CGTTGTATCG GATCCAGAGG ACTCCACAGA TTGTCCGAGG 360

ACTCCAGATA CTCCAAATAG TGACTCTCGT AGTTCTACTA GCAACAATAG ACTGATGGCC 420

TTACAGAAAC AACTGGATAT AGAACTTAAA GTAAAGCAGG GTGCAGAGAA CATGATACAG 480

ATGTACTCAA ATGGCTCTTC AAAGGATCGG AAACTCCATG GTACAGCGCA GCAACTGCTA 540

CAGGATAGCA AGACAAAAAT AGAAGTCATC CGGATGCAGA TTCTTCAGGC CGTCCAGACC 600

AATGAGCTGG CTTTTGATAA TGCAAAACCT GTGATAAGCC CTCTTGAGCT TCGGATGGAA 660

GAATTAAGAC ATCATTTTAA GATAGAATTT GCAGTAGCCG AAGGTGCAAA GAATGTCATG 720

AAGTTACTTG GCTCAGGAAA AGTAACAGAC AGAAAAGCGC TTTCAGAAGC ACAAGCGAGA 780

TTTAATGAAT CAAGTCAGAA GTTGGACCTT TTAAAGTATT CACTAGAGCA AAGGTTAAAT 840

GAGCTCCCCA GGAATCACCC CAAAAGCAGT GTCGTCATTG AGGAGCTTTC ACTGGTTGCG 900

TCACCAACCC TGAGTCCACG GCAGAGCATG CTGTCCACGC AGAACCAGTA TAGCACACTG 960

TCCAAGCCAG CGGCGCTAAC CGGTACTTTG GAAGTTCGTC TTATGGGGTG CCAAGATATC 1020

CTAGAGAATG TACCTGGACG GTCAAAAGCA ACGTCGGTAG CACTACCTGG GTGGAGTCCA 1080

AGTGACAACA GATCGTCTTT TATGAGCAGA ACCAGCAAGA GTAAAAGTGG GAGTAGTCGC 1140

AACCTCCTGA AAACGGATGA CTTGTCCAAT GATGTTTGTG CTGTTTTGAA GCTAGATAAT 1200

ACTGTGGTTG GTCAAACTAG CTGGAAACCC ATTTCAAATC AGTCGTGGGA CCAGAAGTTT 1260

ACACTGGAAC TAGACAGGGT TACTTTTTTT AATCCAGTTA TTGAAAGACG ACCAAAACTC 1320

CAAAGACAAA AGAAAATTTT TTCAAAACAA CAAGGCAAAA CATTTCTCAG AGCTCCTCAG 1380

ATGAATATTA ATATTGCCAC TTGGGGAAGA CTAGTGAGAC GAGCTATTCC CACAGTAAAT 1440

CATTCTGGCA CATTCAGCCC TCAGACTCCT GTGCCTGCAA CAGTGCCAGT CGTTGACGCA 1500

CGCATTCCTG ATCTAGCACC TCCGGCCAGT GACTCTACAG TAACCAAATT GGACTTTGAT 1560

CTTGAACCTG AACCTCCTCC AGCCCCACCT CGTGCTTCTT CCCTTGGAGA GACAGATGAA 1620

TCTTCTGAAT TGAGAGTTTT GGATATACCT GGACAGGGTT CAGAAACTGT TTTCAATATT 1680

GAGAATGACA GAAATAACCT GCGTCCAAAA TCCAAATCTG AATATGAGCT GAGCATTCCT 1740

GACTCAGGTC GAAGCTGCTG GGGTGTTGGA GAGCTTGATG ACAAGAGAGC TCAGCAGAGG 1800

TTTCAGTTCA GTCTACAGGA CTTCAGGTGC TGTGCTGTCT TGGGGAGAGG ACACTTTGGA 1860

AAGGTGCTGT TGGCGGAATA TAAACACACA AATGAAATGT TTGCAATAAA AGCCTTAAAG 1920

AAAGGAGACA TTGTGGCACG AGATGAAGTA GACAGCCTGA TGTGTGAAAA AAGAATTTTT 1980

GAGACTGTGA ATAGTGTCAG GCACCCGTTT CTAGTGAACC TTTTTGCATG TTTCCAAACA 2040

AAAGAACATG TTTGCTTCGT GATGGAGTAT GCTGCAGGTG GTGACCTGAT GATGCACATC 2100

CATACTGATG TCTTCTCTGA ACCCAGAGCT GTATTTTATG CAGCATGTGT AGTTCTTGGG 2160

TTGCAATACT TACATGAACA CAAAATTGTT TATAGAGATT TGAAGTTGGA TAACTTGTTG 2220

CTAGATACAG AAGGCTTTGT GAAAATTGCT GACTTTGGTC TTTGCAAAGA AGGAATGGGG 2280

TATGGAGATA GGACAAGCAC ATTTTGTGGT ACTCCTGAGT TTCTTGCCCC AGAAGTCCTA 2340

ACAGAAACAT CGTACACAAG GGCTGTAGAT TGGTGGGGCC TTGGTGTACT TATATATGAG 2400

ATGCTCGTCG GCGAGTCTCC CTTTCCTGGT GATGATGAAG AAGAGGTTTT TGACAGTATT 2460

GTAAATGACG AAGTAAGGTA TCCAAGGTTC TTATCTACAG AAGCCATTTC CATAATGAGA 2520

AGGCTGCTAA GGAGAAATCC TGAGCGGCGC CTGGGAGCTG GTGAGAAGGA TGCAGAGGAT 2580

GTCAAGAAGC ACCCGTTTTT CCGGCTAACC GACTGGAGTG CTCTAATGGA CAAAAAAGTA 2640

AAGCCACCAT TTGTACCTAC CATTAGAGGA CGGGAAGATG TTAGTAACTT TGATGATGAA 2700

TTTACCTCAG AAGCACCTAT TCTGACTCCA CCTCGAGAAC CAAGGATACT CTTGGAAGAG 2760

GAGCAGGAGA TGTTCCACGA TTTTGACTAC GTTGCTGATT GGTGTTAA 2808

Domain Profile

S: 1     frllavlgrghfgkvllseykstgelyaikalkkgdilardevesllcekrileavssvr  60

         fr++avlgrghfgkvll+eyk+t+e++aikalkkgdi+ardev+sl+cekri+e+v+svr

Q: 608   FRCCAVLGRGHFGKVLLAEYKHTNEMFAIKALKKGDIVARDEVDSLMCEKRIFETVNSVR  667

         9***********************************************************

S: 61    hpflvnllacfqtkehvcfvmeyaaggdlmlhihedvfsepravfyaacvvlglqflhek  120

         hpflvnl+acfqtkehvcfvmeyaaggdlm+hih+dvfsepravfyaacvvlglq+lhe+

Q: 668   HPFLVNLFACFQTKEHVCFVMEYAAGGDLMMHIHTDVFSEPRAVFYAACVVLGLQYLHEH  727

         ************************************************************

S: 121   kivyrdlkldnllldtegyvkiadfglckeglgygdrtstfcgtpeflapevltetsytr  180

         kivyrdlkldnllldteg+vkiadfglckeg+gygdrtstfcgtpeflapevltetsytr

Q: 728   KIVYRDLKLDNLLLDTEGFVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTETSYTR  787

         ************************************************************

S: 181   avdwwglgvllyemlvgespfpgddeeevfdsivndevryprflsveaieilrrllrknp  240

         avdwwglgvl+yemlvgespfpgddeeevfdsivndevryprfls+eai+i+rrllr+np

Q: 788   AVDWWGLGVLIYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSTEAISIMRRLLRRNP  847

         ************************************************************

S: 241   ekrlgaserdaedvkkqpff  260

         e+rlga+e+daedvkk+pff

Q: 848   ERRLGAGEKDAEDVKKHPFF  867

         *******************9

Domain Sequence
(FASTA)

FRCCAVLGRG HFGKVLLAEY KHTNEMFAIK ALKKGDIVAR DEVDSLMCEK RIFETVNSVR 60

HPFLVNLFAC FQTKEHVCFV MEYAAGGDLM MHIHTDVFSE PRAVFYAACV VLGLQYLHEH 120

KIVYRDLKLD NLLLDTEGFV KIADFGLCKE GMGYGDRTST FCGTPEFLAP EVLTETSYTR 180

AVDWWGLGVL IYEMLVGESP FPGDDEEEVF DSIVNDEVRY PRFLSTEAIS IMRRLLRRNP 240

ERRLGAGEKD AEDVKKHPFF 260

Keyword

Acetylation; Alternative splicing; Apoptosis; ATP-binding; Cell adhesion; Cell cycle; Cell division; Cell junction; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Membrane; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; Transcription; Transcription regulation; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0043296	; C:apical junction complex
GO:0032154	; C:cleavage furrow
GO:0005737	; C:cytoplasm
GO:0005856	; C:cytoskeleton
GO:0030027	; C:lamellipodium
GO:0016020	; C:membrane
GO:0030496	; C:midbody
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0042826	; F:histone deacetylase binding
GO:0004697	; F:protein kinase C activity
GO:0004674	; F:protein serine/threonine kinase activity
GO:0043297	; P:apical junction assembly
GO:0006915	; P:apoptotic process
GO:0007155	; P:cell adhesion
GO:0007049	; P:cell cycle
GO:0051301	; P:cell division
GO:0010631	; P:epithelial cell migration
GO:0071777	; P:positive regulation of cell cycle cytokinesis
GO:0045931	; P:positive regulation of mitotic cell cycle
GO:2000145	; P:regulation of cell motility
GO:0006355	; P:regulation of transcription, DNA-dependent
GO:0007165	; P:signal transduction
GO:0006351	; P:transcription, DNA-dependent

KEGG

mmu:109333

;

InterPros

IPR000961	; AGC-kinase_C.
IPR000008	; C2_Ca-dep.
IPR008973	; C2_Ca/lipid-bd_dom_CaLB.
IPR011072	; HR1_rho-bd.
IPR011009	; Kinase-like_dom.
IPR017892	; Pkinase_C.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF02185	; HR1; 3.
PF00069	; Pkinase; 1.
PF00433	; Pkinase_C; 1.

SMARTs

SM00239	; C2; 1.
SM00742	; Hr1; 3.
SM00133	; S_TK_X; 1.
SM00220	; S_TKc; 1.

Prosites

PS51285	; AGC_KINASE_CTER; 1.
PS50004	; C2; FALSE_NEG.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013