EKS-MUM-00527

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00527

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Atypical/TIF1	N/A	N/A	N/A	N/A	N/A

Status

Reviewed

Ensembl Protein

ENSMUSP00000005705

UniProt Accession

Q62318; P70391; Q8C283; Q99PN4;

Protein Name

Transcription intermediary factor 1-beta

Protein Synonyms/Alias

TIF1-beta; E3 SUMO-protein ligase TRIM28; KRAB-A-interacting protein; KRIP-1; Tripartite motif-containing protein 28;

Gene Name

Trim28

Gene Synonyms/Alias

Trim28; Krip1, Tif1b;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000005566	ENSMUSP00000005705	ENSMUST00000005705

Organism

Mus musculus

Functional Description

Nuclear corepressor for KRAB domain-containing zincfinger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteosomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6 (By similarity).

Protein Length

834

Protein Sequence
(FASTA)

MAASAAATAA ASAATAASAA SGSPGSGEGS AGGEKRPAAS SAAAASAAAS SPAGGGGEAQ 60

ELLEHCGVCR ERLRPERDPR LLPCLHSACS ACLGPATPAA ANNSGDGGSA GDGAMVDCPV 120

CKQQCYSKDI VENYFMRDSG SKASSDSQDA NQCCTSCEDN APATSYCVEC SEPLCETCVE 180

AHQRVKYTKD HTVRSTGPAK TRDGERTVYC NVHKHEPLVL FCESCDTLTC RDCQLNAHKD 240

HQYQFLEDAV RNQRKLLASL VKRLGDKHAT LQKNTKEVRS SIRQVSDVQK RVQVDVKMAI 300

LQIMKELNKR GRVLVNDAQK VTEGQQERLE RQHWTMTKIQ KHQEHILRFA SWALESDNNT 360

ALLLSKKLIY FQLHRALKMI VDPVEPHGEM KFQWDLNAWT KSAEAFGKIV AERPGTNSTG 420

PGPMAPPRAP GPLSKQGSGS SQPMEVQEGY GFGSDDPYSS AEPHVSGMKR SRSGEGEVSG 480

LLRKVPRVSL ERLDLDLTSD SQPPVFKVFP GSTTEDYNLI VIERGAAAAA AGQAGTVPPG 540

APGAPPLPGM AIVKEEETEA AIGAPPAAPE GPETKPVLMP LTEGPGAEGP RLASPSGSTS 600

SGLEVVAPEV TSAPVSGPGI LDDSATICRV CQKPGDLVMC NQCEFCFHLD CHLPALQDVP 660

GEEWSCSLCH VLPDLKEEDG SLSLDGADST GVVAKLSPAN QRKCERVLLA LFCHEPCRPL 720

HQLATDSTFS MEQPGGTLDL TLIRARLQEK LSPPYSSPQE FAQDVGRMFK QFNKLTEDKA 780

DVQSIIGLQR FFETRMNDAF GDTKFSAVLV EPPPLNLPSA GLSSQELSGP GDGP 834

Nucleotide Sequence
(FASTA)

ATGGCGGCCT CGGCGGCAGC GACTGCAGCG GCCTCGGCCG CGACGGCCGC CTCGGCGGCC 60

TCTGGTAGCC CAGGGTCGGG CGAGGGCTCG GCGGGCGGTG AGAAGCGTCC GGCTGCTTCC 120

TCAGCCGCGG CGGCCTCTGC AGCCGCGTCG TCCCCTGCGG GGGGCGGTGG CGAGGCGCAG 180

GAGCTTCTGG AGCACTGCGG CGTGTGTCGC GAGCGCCTGC GGCCCGAGCG GGATCCTCGG 240

CTGCTGCCCT GTCTACATTC GGCCTGCAGT GCCTGCCTGG GCCCCGCTAC ACCCGCCGCA 300

GCGAATAATT CGGGGGATGG CGGCTCGGCG GGCGACGGCG CTATGGTGGA TTGTCCAGTG 360

TGCAAACAGC AGTGCTACTC CAAAGACATC GTGGAGAATT ATTTTATGCG TGATAGTGGC 420

AGTAAGGCCT CTTCTGATTC CCAGGATGCT AACCAGTGCT GCACTAGCTG TGAAGATAAT 480

GCCCCAGCCA CTAGCTATTG TGTGGAGTGC TCTGAACCAC TTTGTGAGAC CTGTGTGGAG 540

GCTCACCAGC GGGTGAAATA CACCAAGGAC CACACTGTGC GCTCCACAGG ACCTGCTAAG 600

ACTCGAGATG GAGAGCGAAC AGTCTACTGT AATGTGCACA AGCATGAGCC CCTCGTGCTG 660

TTCTGTGAGA GCTGTGACAC ACTCACCTGC CGCGACTGCC AGCTCAACGC TCACAAGGAC 720

CATCAGTACC AGTTTTTGGA AGATGCAGTG AGGAACCAAC GTAAACTCTT GGCTTCACTG 780

GTGAAACGTC TTGGGGACAA ACATGCCACA CTTCAGAAAA ACACCAAGGA GGTTCGAAGC 840

TCGATCCGCC AGGTGTCTGA TGTGCAGAAG CGAGTGCAGG TTGATGTCAA GATGGCCATT 900

CTGCAGATCA TGAAGGAGCT GAATAAGCGG GGTCGAGTTC TGGTCAATGA TGCCCAGAAG 960

GTGACCGAGG GTCAGCAGGA ACGTCTGGAG CGCCAGCACT GGACCATGAC CAAAATTCAG 1020

AAGCACCAGG AACACATTTT GCGTTTTGCC TCTTGGGCTC TGGAGAGTGA TAACAATACA 1080

GCTCTCTTGC TCTCTAAGAA GCTGATCTAT TTCCAGCTGC ATCGGGCCCT CAAAATGATT 1140

GTGGATCCTG TGGAGCCTCA TGGTGAGATG AAGTTTCAGT GGGATCTCAA TGCCTGGACC 1200

AAGAGTGCTG AAGCCTTTGG CAAGATTGTG GCTGAGCGTC CTGGTACGAA CTCCACAGGT 1260

CCTGGGCCCA TGGCTCCTCC AAGAGCCCCA GGCCCTCTAA GCAAGCAAGG TTCTGGCAGT 1320

AGCCAGCCCA TGGAAGTACA AGAGGGATAT GGCTTTGGGT CAGATGATCC CTATTCAAGT 1380

GCAGAGCCGC ATGTATCAGG CATGAAGCGG TCCCGCTCTG GTGAGGGAGA GGTAAGTGGC 1440

CTCTTAAGGA AGGTGCCACG TGTGAGCCTT GAACGCCTGG ATCTGGACCT CACCTCTGAC 1500

AGCCAGCCAC CAGTCTTCAA GGTCTTTCCT GGAAGCACTA CTGAGGACTA CAATCTGATT 1560

GTTATTGAGC GTGGTGCTGC TGCAGCAGCT GCTGGTCAGG CTGGGACTGT GCCACCAGGA 1620

GCCCCTGGTG CCCCACCCCT TCCTGGCATG GCCATTGTCA AGGAAGAAGA GACAGAAGCT 1680

GCTATTGGAG CTCCCCCGGC TGCCCCCGAG GGTCCTGAAA CCAAGCCTGT GTTGATGCCT 1740

CTGACTGAAG GTCCTGGTGC CGAGGGACCT CGTCTAGCTT CACCTAGTGG CAGTACCAGC 1800

TCAGGCTTGG AGGTGGTGGC TCCTGAGGTT ACCTCAGCCC CAGTAAGTGG GCCAGGTATC 1860

CTGGATGACA GTGCCACTAT CTGCCGTGTC TGCCAGAAAC CAGGTGACCT GGTCATGTGT 1920

AACCAGTGCG AATTTTGCTT CCACCTGGAT TGCCACCTCC CTGCCCTGCA GGATGTTCCA 1980

GGGGAGGAAT GGAGTTGCTC ACTCTGCCAC GTGCTCCCTG ACCTAAAGGA GGAAGATGGA 2040

AGCCTCAGCC TGGATGGAGC AGATAGCACT GGTGTGGTAG CTAAACTCTC ACCAGCCAAC 2100

CAGCGGAAAT GTGAGCGTGT TCTCCTGGCC CTGTTCTGCC ATGAACCATG CCGTCCCTTG 2160

CATCAGCTGG CTACCGACTC TACATTCTCC ATGGAGCAGC CTGGTGGTAC CCTAGACCTG 2220

ACCTTGATTC GTGCTCGCCT CCAAGAGAAG CTGTCACCTC CTTATAGCTC CCCCCAGGAG 2280

TTTGCTCAAG ATGTGGGCCG CATGTTCAAA CAGTTCAACA AGCTGACTGA GGACAAGGCA 2340

GATGTTCAGT CCATCATCGG CTTGCAGCGC TTCTTTGAGA CACGCATGAA TGATGCCTTT 2400

GGTGACACCA AGTTTTCTGC TGTGCTGGTA GAACCACCAC CATTGAACCT TCCCAGTGCT 2460

GGCCTAAGTT CTCAGGAGCT CTCTGGCCCT GGTGATGGCC CCTGA 2505

Domain Profile

N/A

Domain Sequence
(FASTA)

N/A

Keyword

Acetylation; Alternative splicing; Bromodomain; Complete proteome; Isopeptide bond; Ligase; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Ubl conjugation; Ubl conjugation pathway; Zinc; Zinc-finger.

Sequence Source

Ensembl

Orthology

Ortholog group

Anolis carolinensis"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Macropus eugenii"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>

Gene Ontology

GO:0005719	; C:nuclear euchromatin
GO:0005720	; C:nuclear heterochromatin
GO:0005654	; C:nucleoplasm
GO:0003677	; F:DNA binding
GO:0004672	; F:protein kinase activity
GO:0003713	; F:transcription coactivator activity
GO:0003714	; F:transcription corepressor activity
GO:0004842	; F:ubiquitin-protein ligase activity
GO:0008270	; F:zinc ion binding
GO:0060028	; P:convergent extension involved in axis elongation
GO:0006281	; P:DNA repair
GO:0060669	; P:embryonic placenta morphogenesis
GO:0001837	; P:epithelial to mesenchymal transition
GO:0000122	; P:negative regulation of transcription from RNA polymerase II promoter
GO:0045739	; P:positive regulation of DNA repair
GO:0045893	; P:positive regulation of transcription, DNA-dependent
GO:0046777	; P:protein autophosphorylation
GO:0051259	; P:protein oligomerization
GO:0016925	; P:protein sumoylation
GO:0006351	; P:transcription, DNA-dependent

KEGG

mmu:21849

;

InterPros

IPR003649	; Bbox_C.
IPR001487	; Bromodomain.
IPR019786	; Zinc_finger_PHD-type_CS.
IPR000315	; Znf_B-box.
IPR011011	; Znf_FYVE_PHD.
IPR001965	; Znf_PHD.
IPR019787	; Znf_PHD-finger.
IPR001841	; Znf_RING.
IPR013083	; Znf_RING/FYVE/PHD.

Pfam

PF00628	; PHD; 1.
PF00643	; zf-B_box; 2.

SMARTs

SM00502	; BBC; 1.
SM00336	; BBOX; 2.
SM00297	; BROMO; 1.
SM00249	; PHD; 1.
SM00184	; RING; 2.

Prosites

PS00633	; BROMODOMAIN_1; FALSE_NEG.
PS50014	; BROMODOMAIN_2; FALSE_NEG.
PS50119	; ZF_BBOX; 2.
PS01359	; ZF_PHD_1; 1.
PS50016	; ZF_PHD_2; 1.
PS00518	; ZF_RING_1; FALSE_NEG.
PS50089	; ZF_RING_2; 1.

Prints

Created Date

20-Feb-2013