EKS-MUM-00435

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00435

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/PDGFR	595.7	7.9E-179	592	926	335

Status

Reviewed

Ensembl Protein

ENSMUSP00000005815

UniProt Accession

P05532; Q61415; Q61416; Q61417; Q6LEE9; Q6QJB7; Q6QJB8; Q7TS86; Q8C8K9;

Protein Name

Mast/stem cell growth factor receptor Kit

Protein Synonyms/Alias

SCFR; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit;

Gene Name

Kit

Gene Synonyms/Alias

Kit; Sl;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000005672	ENSMUSP00000116465	ENSMUST00000144270
ENSMUSG00000005672	ENSMUSP00000005815	ENSMUST00000005815

Organism

Mus musculus

Functional Description

Tyrosine-protein kinase that acts as cell-surfacereceptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.

Protein Length

979

Protein Sequence
(FASTA)

MRGARGAWDL LCVLLVLLRG QTATSQPSAS PGEPSPPSIH PAQSELIVEA GDTLSLTCID 60

PDFVRWTFKT YFNEMVENKK NEWIQEKAEA TRTGTYTCSN SNGLTSSIYV FVRDPAKLFL 120

VGLPLFGKED SDALVRCPLT DPQVSNYSLI ECDGKSLPTD LTFVPNPKAG ITIKNVKRAY 180

HRLCVRCAAQ RDGTWLHSDK FTLKVRAAIK AIPVVSVPET SHLLKKGDTF TVVCTIKDVS 240

TSVNSMWLKM NPQPQHIAQV KHNSWHRGDF NYERQETLTI SSARVDDSGV FMCYANNTFG 300

SANVTTTLKV VEKGFINISP VKNTTVFVTD GENVDLVVEY EAYPKPEHQQ WIYMNRTSAN 360

KGKDYVKSDN KSNIRYVNQL RLTRLKGTEG GTYTFLVSNS DASASVTFNV YVNTKPEILT 420

YDRLINGMLQ CVAEGFPEPT IDWYFCTGAE QRCTTPVSPV DVQVQNVSVS PFGKLVVQSS 480

IDSSVFRHNG TVECKASNDV GKSSAFFNFA FKGNNKEQIQ AHTLFTPLLI GFVVAAGAMG 540

IIVMVLTYKY LQKPMYEVQW KVVEEINGNN YVYIDPTQLP YDHKWEFPRN RLSFGKTLGA 600

GAFGKVVEAT AYGLIKSDAA MTVAVKMLKP SAHLTEREAL MSELKVLSYL GNHMNIVNLL 660

GACTVGGPTL VITEYCCYGD LLNFLRRKRD SFIFSKQEEQ AEAALYKNLL HSTEPSCDSS 720

NEYMDMKPGV SYVVPTKTDK RRSARIDSYI ERDVTPAIME DDELALDLDD LLSFSYQVAK 780

GMAFLASKNC IHRDLAARNI LLTHGRITKI CDFGLARDIR NDSNYVVKGN ARLPVKWMAP 840

ESIFSCVYTF ESDVWSYGIF LWELFSLGSS PYPGMPVDSK FYKMIKEGFR MVSPEHAPAE 900

MYDVMKTCWD ADPLKRPTFK QVVQLIEKQI SDSTKHIYSN LANCNPNPEN PVVVDHSVRV 960

NSVGSSASST QPLLVHEDA 979

Nucleotide Sequence
(FASTA)

ATGAGAGGCG CTCGCGGCGC CTGGGATCTG CTCTGCGTCC TGTTGGTCCT GCTCCGTGGC 60

CAGACAGCCA CGTCTCAGCC ATCTGCAAGT CCAGGGGAGC CGTCTCCGCC ATCCATCCAT 120

CCAGCACAAT CAGAGTTAAT AGTTGAAGCT GGCGACACCC TCAGCCTGAC GTGCATTGAT 180

CCCGACTTTG TCAGATGGAC TTTCAAGACC TATTTCAATG AAATGGTTGA GAATAAAAAA 240

AATGAATGGA TCCAGGAAAA AGCCGAGGCC ACTCGCACGG GCACATACAC GTGCAGCAAC 300

AGCAATGGCC TCACGAGTTC TATTTACGTG TTTGTTAGAG ATCCTGCCAA ACTTTTCCTG 360

GTTGGCCTTC CCTTGTTTGG CAAAGAAGAC AGCGACGCGC TGGTCCGCTG CCCTCTGACA 420

GACCCACAGG TGTCCAATTA TTCCCTCATC GAGTGTGATG GGAAATCTCT CCCCACGGAC 480

CTGACGTTTG TCCCAAACCC CAAGGCTGGC ATCACCATCA AAAACGTGAA GCGCGCCTAC 540

CACCGGCTCT GTGTCCGCTG TGCTGCTCAG CGTGACGGTA CATGGCTGCA TTCTGACAAA 600

TTCACCCTCA AAGTGCGGGC AGCCATCAAG GCTATCCCTG TTGTGTCTGT GCCTGAAACA 660

AGTCACCTCC TTAAGAAAGG GGACACATTT ACGGTGGTGT GCACCATAAA AGATGTGTCT 720

ACATCCGTGA ACTCCATGTG GCTAAAGATG AACCCTCAGC CTCAGCACAT AGCCCAGGTA 780

AAGCACAATA GCTGGCACCG GGGTGACTTC AATTATGAAC GCCAGGAGAC GCTGACTATC 840

AGCTCGGCAA GAGTTGACGA TTCTGGAGTG TTCATGTGTT ATGCCAATAA TACTTTTGGA 900

TCAGCAAATG TCACAACAAC CTTGAAAGTA GTAGAAAAAG GATTCATCAA CATCTCCCCT 960

GTGAAGAACA CTACAGTATT TGTAACCGAT GGAGAAAACG TAGATTTGGT TGTTGAATAC 1020

GAGGCCTACC CCAAACCCGA GCACCAGCAG TGGATATATA TGAACAGGAC CTCGGCTAAC 1080

AAAGGGAAGG ATTATGTCAA ATCTGATAAC AAAAGCAACA TCAGATATGT GAACCAACTT 1140

CGCCTGACCA GATTAAAAGG CACAGAAGGA GGCACTTATA CCTTTCTGGT GTCCAACTCT 1200

GATGCCAGTG CTTCCGTGAC ATTCAACGTT TACGTGAACA CAAAACCAGA AATCCTGACG 1260

TACGACAGGC TCATAAATGG CATGCTCCAG TGTGTGGCAG AGGGATTCCC GGAGCCCACA 1320

ATAGATTGGT ATTTTTGTAC AGGAGCAGAG CAAAGGTGTA CCACTCCTGT CTCACCAGTG 1380

GACGTACAGG TCCAGAATGT ATCTGTGTCA CCATTTGGAA AACTGGTGGT TCAGAGTTCC 1440

ATAGACTCCA GCGTCTTCCG GCACAACGGC ACGGTGGAGT GTAAGGCCTC CAACGATGTG 1500

GGCAAGAGTT CCGCCTTCTT TAACTTTGCA TTTAAAGGTA ACAACAAAGA GCAAATCCAG 1560

GCCCACACTC TGTTCACGCC GCTGCTCATT GGCTTTGTGG TTGCAGCTGG CGCGATGGGG 1620

ATCATTGTGA TGGTGCTCAC CTACAAATAT TTGCAGAAAC CCATGTATGA AGTACAATGG 1680

AAGGTTGTCG AGGAGATAAA TGGAAACAAT TATGTTTACA TAGACCCGAC GCAACTTCCT 1740

TATGATCACA AATGGGAGTT TCCCAGAAAC AGGCTGAGTT TTGGAAAGAC ATTGGGAGCT 1800

GGTGCCTTCG GGAAGGTCGT TGAGGCCACT GCATATGGCT TGATTAAGTC GGATGCTGCC 1860

ATGACAGTTG CCGTGAAGAT GCTCAAACCA AGTGCCCATT TAACAGAAAG AGAGGCCCTA 1920

ATGTCGGAAC TGAAGGTCCT GAGCTACCTG GGCAATCACA TGAATATTGT GAACCTGCTT 1980

GGCGCATGCA CGGTGGGAGG GCCCACCCTG GTCATTACAG AATATTGTTG CTATGGTGAT 2040

CTTTTGAATT TTTTGAGAAG GAAGCGTGAC TCGTTTATTT TCTCAAAGCA AGAAGAGCAG 2100

GCAGAAGCGG CACTTTATAA GAACCTTCTG CACTCAACGG AGCCTTCCTG TGACAGTTCA 2160

AATGAATATA TGGACATGAA GCCTGGCGTT TCCTACGTGG TGCCAACCAA GACAGACAAG 2220

AGGAGATCCG CAAGAATAGA CTCGTACATA GAAAGAGACG TGACTCCTGC CATCATGGAA 2280

GATGACGAGC TGGCTCTGGA CCTGGATGAT TTGCTGAGCT TCTCCTACCA GGTGGCCAAG 2340

GGCATGGCGT TCCTCGCCTC CAAGAATTGT ATTCACAGAG ATTTGGCAGC CAGGAATATC 2400

CTCCTCACTC ACGGGCGGAT CACAAAGATT TGCGATTTCG GGCTAGCCAG AGACATCAGG 2460

AATGATTCGA ATTACGTGGT CAAAGGAAAT GCACGACTGC CCGTGAAGTG GATGGCACCA 2520

GAGAGCATTT TCAGCTGCGT GTACACATTT GAAAGTGATG TCTGGTCCTA TGGGATTTTC 2580

CTCTGGGAGC TCTTCTCCTT AGGAAGCAGC CCCTACCCAG GGATGCCGGT CGACTCCAAG 2640

TTCTACAAGA TGATCAAGGA AGGCTTCCGG ATGGTCAGCC CGGAGCACGC GCCTGCCGAA 2700

ATGTATGACG TCATGAAGAC TTGCTGGGAC GCTGACCCCT TGAAAAGGCC AACATTCAAG 2760

CAGGTTGTCC AACTTATTGA GAAGCAGATC TCGGACAGCA CCAAGCACAT TTACTCCAAC 2820

TTGGCAAACT GCAACCCCAA CCCAGAGAAC CCCGTGGTGG TGGACCATTC CGTGAGGGTC 2880

AACTCGGTGG GCAGCAGCGC CTCTTCTACG CAGCCCCTGC TCGTGCACGA AGATGCCTGA 2940

Domain Profile

S: 1     lelGkvlGsGafGkvveatayGlsksdavlkvavkmlkstarssekealmselkilshlG  60

         l++Gk+lG+GafGkvveatayGl+ksda+++vavkmlk++a+ +e+ealmselk+ls+lG

Q: 592   LSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLG  651

         79**********************************************************

S: 61    ehenivnllGactkgGpiyviteyccyGdllnylrrkresflkkslekkkekeeeldska  120

         +h+nivnllGact+gGp++viteyccyGdlln+lrrkr+sf+   ++k++e++e++ +k+

Q: 652   NHMNIVNLLGACTVGGPTLVITEYCCYGDLLNFLRRKRDSFI---FSKQEEQAEAALYKN  708

         ******************************************...888899999999999

S: 121   lladkslrsksslssesseeyvdlkkdvsdvssskadklasekkksalerevkaaleeed  180

         l     l+s++ ++ +ss+ey+d+k++vs+v ++k+dk++s++++s++er+v++a++e+d

Q: 709   L-----LHSTEPSC-DSSNEYMDMKPGVSYVVPTKTDKRRSARIDSYIERDVTPAIMEDD  762

         9.....89988887.999******************************************

S: 181   sevleledllsfsyqvakGmeflaskncvhrdlaarnvllteGkvvkicdfGlardimkd  240

         + +l+l+dllsfsyqvakGm+flasknc+hrdlaarn+llt+G+++kicdfGlardi++d

Q: 763   ELALDLDDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIRND  822

         ************************************************************

S: 241   snyvvkGnarlpvkwmapesifdslytvksdvwsyGillweifslGstpypgllvdskfy  300

         snyvvkGnarlpvkwmapesif+++yt++sdvwsyGi+lwe+fslGs+pypg++vdskfy

Q: 823   SNYVVKGNARLPVKWMAPESIFSCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFY  882

         ************************************************************

S: 301   klikeGyrmaqpayateeiyeimqkcweaepekrpsfkqlvell  344

         k+ikeG+rm +p++a++e+y++m++cw+a+p krp+fkq+v+l+

Q: 883   KMIKEGFRMVSPEHAPAEMYDVMKTCWDADPLKRPTFKQVVQLI  926

         *****************************************996

Domain Sequence
(FASTA)

LSFGKTLGAG AFGKVVEATA YGLIKSDAAM TVAVKMLKPS AHLTEREALM SELKVLSYLG 60

NHMNIVNLLG ACTVGGPTLV ITEYCCYGDL LNFLRRKRDS FIFSKQEEQA EAALYKNLLH 120

STEPSCDSSN EYMDMKPGVS YVVPTKTDKR RSARIDSYIE RDVTPAIMED DELALDLDDL 180

LSFSYQVAKG MAFLASKNCI HRDLAARNIL LTHGRITKIC DFGLARDIRN DSNYVVKGNA 240

RLPVKWMAPE SIFSCVYTFE SDVWSYGIFL WELFSLGSSP YPGMPVDSKF YKMIKEGFRM 300

VSPEHAPAEM YDVMKTCWDA DPLKRPTFKQ VVQLI 335

Keyword

3D-structure; Alternative splicing; ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Disease mutation; Disulfide bond; Glycoprotein; Immunoglobulin domain; Kinase; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Proto-oncogene; Receptor; Reference proteome; Repeat; Signal; Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0009897	; C:external side of plasma membrane
GO:0005615	; C:extracellular space
GO:0016021	; C:integral to membrane
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0019955	; F:cytokine binding
GO:0046872	; F:metal ion binding
GO:0005020	; F:stem cell factor receptor activity
GO:0031532	; P:actin cytoskeleton reorganization
GO:0000187	; P:activation of MAPK activity
GO:0060326	; P:cell chemotaxis
GO:0097067	; P:cellular response to thyroid hormone stimulus
GO:0002371	; P:dendritic cell cytokine production
GO:0050910	; P:detection of mechanical stimulus involved in sensory perception of sound
GO:0048565	; P:digestive tract development
GO:0035162	; P:embryonic hemopoiesis
GO:0030218	; P:erythrocyte differentiation
GO:0038162	; P:erythropoietin-mediated signaling pathway
GO:0038093	; P:Fc receptor signaling pathway
GO:0035234	; P:germ cell programmed cell death
GO:0006687	; P:glycosphingolipid metabolic process
GO:0002327	; P:immature B cell differentiation
GO:0006954	; P:inflammatory response
GO:0007243	; P:intracellular protein kinase cascade
GO:0030032	; P:lamellipodium assembly
GO:0002320	; P:lymphoid progenitor cell differentiation
GO:0008584	; P:male gonad development
GO:0002551	; P:mast cell chemotaxis
GO:0032762	; P:mast cell cytokine production
GO:0043303	; P:mast cell degranulation
GO:0060374	; P:mast cell differentiation
GO:0035855	; P:megakaryocyte development
GO:0097326	; P:melanocyte adhesion
GO:0030318	; P:melanocyte differentiation
GO:0097324	; P:melanocyte migration
GO:0002318	; P:myeloid progenitor cell differentiation
GO:0043069	; P:negative regulation of programmed cell death
GO:0001541	; P:ovarian follicle development
GO:0018108	; P:peptidyl-tyrosine phosphorylation
GO:0008284	; P:positive regulation of cell proliferation
GO:0010628	; P:positive regulation of gene expression
GO:0043406	; P:positive regulation of MAP kinase activity
GO:0043410	; P:positive regulation of MAPK cascade
GO:0051091	; P:positive regulation of sequence-specific DNA binding transcription factor activity
GO:0042511	; P:positive regulation of tyrosine phosphorylation of Stat1 protein
GO:0042517	; P:positive regulation of tyrosine phosphorylation of Stat3 protein
GO:0042523	; P:positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0046777	; P:protein autophosphorylation
GO:0008360	; P:regulation of cell shape
GO:0048070	; P:regulation of developmental pigmentation
GO:0009314	; P:response to radiation
GO:0007286	; P:spermatid development
GO:0048863	; P:stem cell differentiation
GO:0030217	; P:T cell differentiation

KEGG

mmu:16590

;

InterPros

IPR007110	; Ig-like.
IPR013783	; Ig-like_fold.
IPR013098	; Ig_I-set.
IPR003599	; Ig_sub.
IPR003598	; Ig_sub2.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.
IPR016243	; Tyr_kinase_CSF1/PDGF_rcpt.
IPR001824	; Tyr_kinase_rcpt_3_CS.

Pfam

PF07679	; I-set; 1.
PF07714	; Pkinase_Tyr; 1.

SMARTs

SM00409	; IG; 2.
SM00408	; IGc2; 1.
SM00219	; TyrKc; 1.

Prosites

PS50835	; IG_LIKE; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS00240	; RECEPTOR_TYR_KIN_III; 1.

Prints

Created Date

20-Feb-2013