EKS-MUM-00256

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00256

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CAMK/CAMKL	325.1	6.5E-97	57	307	251

Status

Reviewed

Ensembl Protein

ENSMUSP00000080769

UniProt Accession

Q8BZN4; Q80ZW3; Q8CIC0; Q9DBV0;

Protein Name

NUAK family SNF1-like kinase 2

Protein Synonyms/Alias

Omphalocele kinase 2;

Gene Name

Nuak2

Gene Synonyms/Alias

Nuak2; Omphk2;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000009772	ENSMUSP00000072039	ENSMUST00000072177
ENSMUSG00000009772	ENSMUSP00000080769	ENSMUST00000082125

Organism

Mus musculus

Functional Description

Stress-activated kinase involved in tolerance to glucosestarvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95- activated tumor cells. Able to phosphorylate 'Ser-464' of LATS1 (By similarity).

Protein Length

631

Protein Sequence
(FASTA)

MESVALLQRP SQAPSASALA SESARPLADG LIKSPKPLMK KQAVKRHHHK HNLRHRYEFL 60

ETLGKGTYGK VKKARESSGR LVAIKSIRKD KIKDEQDLLH IRREIEIMSS LNHPHIIAIH 120

EVFENSSKIV IVMEYASRGD LYDYISERPR LSERDARHFF RQIVSALHYC HQNGIVHRDL 180

KLENILLDAN GNIKIADFGL SNLYHKGKFL QTFCGSPLYA SPEIVNGKPY VGPEVDSWSL 240

GVLLYILVHG TMPFDGQDHK TLVKQISNGA YREPPKPSDA CGLIRWLLMV NPTRRATLED 300

VASHWWVNWG YTTGVGEQEA LREGGHPSGD FGRASMADWL RRSSRPLLEN GAKVCSFFKQ 360

HVPGGGSTVP GLERQHSLKK SRKENDMAQN LQGDPAEDTS SRPGKSSLKL PKGILKKKSS 420

TSSGEVQEDP QELRPVPDTP GQPVPAVSLL PRKGILKKSR QRESGYYSSP EPSESGELLD 480

ASDVFVSGDP VEQKSPQASG LLLHRKGILK LNGKFSRTAL EGTTPSTFGS LDQLASSHPA 540

ARPSRPSGAV SEDSILSSES FDQLDLPERL PETPLRGCVS VDNLRGLEQP PSEGLKRWWQ 600

ESLGDSCFSL TDCQEVTAAY RQALGICSKL S 631

Nucleotide Sequence
(FASTA)

ATGGAGTCGG TGGCCTTACT CCAGCGCCCG AGCCAGGCTC CCTCGGCCTC CGCCCTGGCC 60

TCGGAGAGCG CCCGGCCGCT GGCGGACGGG CTCATCAAGT CGCCTAAACC TCTGATGAAG 120

AAGCAGGCGG TGAAGCGGCA CCATCACAAA CACAACCTGC GGCACCGCTA CGAGTTCCTG 180

GAGACGCTGG GCAAGGGCAC CTACGGGAAG GTGAAGAAGG CACGAGAGAG CTCGGGGCGT 240

CTGGTGGCCA TCAAGTCCAT CAGGAAAGAC AAAATCAAAG ATGAGCAGGA TCTGCTGCAC 300

ATACGGAGGG AGATTGAGAT CATGTCTTCA CTCAACCACC CCCACATCAT TGCCATCCAT 360

GAAGTGTTTG AGAATAGCAG CAAGATTGTG ATTGTCATGG AGTATGCCAG CCGAGGCGAT 420

CTGTATGATT ACATCAGTGA GCGGCCACGG CTGAGTGAGC GGGACGCCAG GCATTTCTTC 480

CGACAGATCG TGTCTGCCCT GCACTACTGC CACCAGAACG GGATCGTTCA CCGAGATCTC 540

AAGCTGGAAA ACATCCTTCT AGATGCCAAT GGAAACATCA AGATTGCTGA CTTTGGCCTC 600

TCCAACCTGT ACCACAAAGG CAAGTTCCTC CAGACGTTCT GTGGGAGCCC TCTCTACGCC 660

TCGCCTGAGA TAGTCAACGG GAAGCCCTAT GTGGGCCCAG AGGTGGACAG CTGGTCTCTG 720

GGCGTTCTCC TGTACATCCT GGTGCATGGC ACCATGCCCT TTGACGGGCA GGATCATAAA 780

ACACTGGTGA AGCAAATCAG TAACGGGGCT TACCGTGAGC CGCCCAAGCC GTCCGATGCC 840

TGTGGCCTGA TCCGGTGGCT GTTAATGGTG AACCCCACCC GTCGGGCCAC ACTGGAGGAT 900

GTAGCCAGTC ATTGGTGGGT CAACTGGGGT TACACCACCG GAGTCGGGGA ACAGGAAGCC 960

CTGCGTGAGG GTGGGCACCC TAGTGGTGAC TTTGGCCGGG CCTCCATGGC GGACTGGTTA 1020

CGTCGCTCCT CGCGCCCCCT CCTGGAGAAT GGAGCCAAGG TGTGCAGCTT CTTCAAGCAG 1080

CACGTGCCGG GAGGTGGAAG CACTGTACCT GGGCTGGAGC GGCAACATTC TCTTAAGAAG 1140

TCCCGAAAGG AGAATGACAT GGCTCAAAAT CTGCAAGGTG ACCCGGCTGA GGATACCTCT 1200

TCTCGCCCTG GCAAGAGCAG CCTTAAGCTT CCGAAAGGCA TTCTCAAGAA AAAGTCCTCT 1260

ACCTCGTCAG GGGAGGTACA GGAGGACCCT CAGGAACTCA GACCGGTGCC TGATACTCCA 1320

GGGCAGCCTG TCCCTGCTGT ATCCCTGCTC CCAAGGAAAG GCATCCTTAA GAAGTCTCGA 1380

CAGCGTGAAT CTGGTTACTA CTCCTCTCCA GAGCCCAGCG AGTCTGGGGA ACTCTTAGAC 1440

GCCAGTGATG TGTTTGTGAG TGGGGACCCC GTGGAGCAGA AGTCTCCACA GGCTTCAGGG 1500

CTCCTCCTCC ACCGCAAGGG CATTCTCAAA CTCAATGGCA AGTTCTCCCG CACAGCCTTA 1560

GAAGGCACTA CCCCTAGCAC CTTTGGCTCC CTGGACCAAC TGGCCTCCTC CCATCCTGCA 1620

GCCCGGCCCA GCCGCCCCTC AGGGGCTGTG AGTGAGGACA GCATCCTGTC CTCCGAGTCC 1680

TTTGACCAAT TGGACTTGCC TGAACGTCTT CCCGAAACCC CACTGAGGGG CTGTGTGTCT 1740

GTGGACAACC TGAGGGGGCT TGAGCAGCCT CCCTCAGAAG GTCTGAAGCG ATGGTGGCAG 1800

GAATCCTTGG GGGATAGCTG CTTTTCTCTG ACAGACTGCC AAGAGGTGAC TGCAGCCTAC 1860

AGACAAGCCC TAGGAATCTG CTCAAAGCTC AGCTGA 1896

Domain Profile

S: 1     ykllktlGegsfgkVklakhketkekvavKiikkkkl.seeslekikrEieilkklkhpn  59

         y++l+tlG+g++gkVk+a+++  ++ va+K i+k+k+ +e+ l +i+rEiei+++l+hp+

Q: 57    YEFLETLGKGTYGKVKKARES-SGRLVAIKSIRKDKIkDEQDLLHIRREIEIMSSLNHPH  115

         99***************9986.799**********996667788****************

S: 60    iikllevietkkklylvleyasggeLfdyieekgrlkekearklfkqlvsavkYlhskgi  119

         ii ++ev+e+++k+++v+eyas+g+L+dyi+e+ rl+e++ar++f+q+vsa++Y+h++gi

Q: 116   IIAIHEVFENSSKIVIVMEYASRGDLYDYISERPRLSERDARHFFRQIVSALHYCHQNGI  175

         ************************************************************

S: 120   vHrDlKpeNllldkkenikiaDFGlanlfkegklletfcGsppYaaPellkgkkYegpkv  179

         vHrDlK+eN+lld+++nikiaDFGl+nl+++gk l+tfcGsp Ya+Pe+++gk+Y gp+v

Q: 176   VHRDLKLENILLDANGNIKIADFGLSNLYHKGKFLQTFCGSPLYASPEIVNGKPYVGPEV  235

         ************************************************************

S: 180   DvWslGvvLyalvtgklPfdeenlkellekilkgkleipkklskelesLlrklLvvdpek  239

         D WslGv+Ly+lv g++Pfd+++ k+l+++i++g ++ p k s ++  L+r lL+v+p++

Q: 236   DSWSLGVLLYILVHGTMPFDGQDHKTLVKQISNGAYREPPKPS-DACGLIRWLLMVNPTR  294

         ****************************************966.5668************

S: 240   Ratieeilkhewl  252

         Rat+e++ +h w+

Q: 295   RATLEDVASHWWV  307

         ************6

Domain Sequence
(FASTA)

YEFLETLGKG TYGKVKKARE SSGRLVAIKS IRKDKIKDEQ DLLHIRREIE IMSSLNHPHI 60

IAIHEVFENS SKIVIVMEYA SRGDLYDYIS ERPRLSERDA RHFFRQIVSA LHYCHQNGIV 120

HRDLKLENIL LDANGNIKIA DFGLSNLYHK GKFLQTFCGS PLYASPEIVN GKPYVGPEVD 180

SWSLGVLLYI LVHGTMPFDG QDHKTLVKQI SNGAYREPPK PSDACGLIRW LLMVNPTRRA 240

TLEDVASHWW V 251

Keyword

Alternative splicing; Apoptosis; ATP-binding; Complete proteome; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Dasypus novemcinctus"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005524	; F:ATP binding
GO:0000287	; F:magnesium ion binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0030036	; P:actin cytoskeleton organization
GO:0006915	; P:apoptotic process
GO:0042149	; P:cellular response to glucose starvation
GO:0043066	; P:negative regulation of apoptotic process

KEGG

mmu:74137

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013