EKS-MUM-00423

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00423

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
STE/STE11	348.5	7.3E-104	1332	1590	259

Status

Reviewed

Ensembl Protein

ENSMUSP00000086459

UniProt Accession

O08648; O08649; O70124; Q6PDG6;

Protein Name

Mitogen-activated protein kinase kinase kinase 4

Protein Synonyms/Alias

MAPK/ERK kinase kinase 4; MEK kinase 4; MEKK 4;

Gene Name

Map3k4

Gene Synonyms/Alias

Map3k4; Mekk4;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000014426	ENSMUSP00000086459	ENSMUST00000089058

Organism

Mus musculus

Functional Description

Component of a protein kinase signal transductioncascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6.

Protein Length

1597

Protein Sequence
(FASTA)

MRDAIAEPVP PPALADTPAA AMEELRPAPP PQPEPDPECC PAARQECMLG ESARKSMESD 60

PEDFSDETNT ETLYGTSPPS TPRQMKRLSA KHQRNSAGRP ASRSNLKEKM NTPSQSPHKD 120

LGKGVETVEE YSYKQEKKIR ATLRTTERDH KKNAQCSFML DSVAGSLPKK SIPDVDLNKP 180

YLSLGCSNAK LPVSMPMPIA RTARQTSRTD CPADRLKFFE TLRLLLKLTS VSKKKDREQR 240

GQENTAAFWF NRSNELIWLE LQAWHAGRTI NDQDLFLYTA RQAIPDIINE ILTFKVNYGS 300

IAFSSNGAGF NGPLVEGQCR TPQETNRVGC SSYHEHLQRQ RVSFEQVKRI MELLEYMEAL 360

YPSLQALQKD YERYAAKDFE DRVQALCLWL NITKDLNQKL RIMGTVLGIK NLSDIGWPVF 420

EIPSPRPSKG YEPEDEVEDT EVELRELESG TEESDEEPTP SPRVPELRLS TDAILDSRSQ 480

GCVSRKLERL ESEEDSIGWG TADCGPEASR HCLTSIYRPF VDKALKQMGL RKLILRLHKL 540

MNGSLQRARV ALVKDDRPVE FSDFPGPMWG SDYVQLSGTP PSSEQKCSAV SWEELRAMDL 600

PSFEPAFLVL CRVLLNVIHE CLKLRLEQRP AGEPSLLSIK QLVRECKEVL KGGLLMKQYY 660

QFMLQEVLGG LEKTDCNMDA FEEDLQKMLM VYFDYMRSWI QMLQQLPQAS HSLKNLLEEE 720

WNFTKEITHY IRGGEAQAGK LFCDIAGMLL KSTGSFLESG LQESCAELWT SADDNGAADE 780

LRRSVIEISR ALKELFHEAR ERASKALGFA KMLRKDLEIA AEFVLSASAR ELLDALKAKQ 840

YVKVQIPGLE NLHVFVPDSL AEEKKIILQL LNAATGKDCS KDPDDVFMDA FLLLTKHGDR 900

ARDSEDGWGT WEARAVKIVP QVETVDTLRS MQVDNLLLVV MESAHLVLQR KAFQQSIEGL 960

MTVRHEQTSS QPIIAKGLQQ LKNDALELCN RISDAIDRVD HMFTLEFDAE VEESESATLQ 1020

QYYREAMIQG YNFGFEYHKE VVRLMSGEFR QKIGDKYISF AQKWMNYVLT KCESGRGTRP 1080

RWATQGFDFL QAIEPAFISA LPEDDFLSLQ ALMNECIGHV IGKPHSPVTA IHRNSPRPVK 1140

VPRCHSDPPN PHLIIPTPEG FSTRSVPSDA RTHGNSVAAA AAVAAAATTA AGRPGPGGGD 1200

SVPAKPVNTA PDTRGSSVPE NDRLASIAAE LQFRSLSRHS SPTEERDEPA YPRSDSSGST 1260

RRSWELRTLI SQTKDSASKQ GPIEAIQKSV RLFEERRYRE MRRKNIIGQV CDTPKSYDNV 1320

MHVGLRKVTF KWQRGNKIGE GQYGKVYTCI SVDTGELMAM KEIRFQPNDH KTIKETADEL 1380

KIFEGIKHPN LVRYFGVELH REEMYIFMEY CDEGTLEEVS RLGLQEHVIR LYTKQITVAI 1440

NVLHEHGIVH RDIKGANIFL TSSGLIKLGD FGCSVKLKNN AQTMPGEVNS TLGTAAYMAP 1500

EVITRAKGEG HGRAADIWSL GCVVIEMVTG KRPWHEYEHN FQIMYKVGMG HKPPIPERLS 1560

PEGKAFLSHC LESDPKIRWT ASQLLDHAFV KVCTDEE 1597

Nucleotide Sequence
(FASTA)

ATGAGAGACG CCATCGCCGA GCCGGTGCCC CCTCCTGCCC TCGCCGACAC CCCTGCAGCC 60

GCCATGGAGG AGCTGCGGCC AGCACCGCCG CCACAGCCCG AGCCGGATCC GGAGTGCTGC 120

CCAGCGGCGA GGCAGGAGTG CATGTTGGGA GAGTCGGCTC GCAAAAGTAT GGAATCCGAT 180

CCAGAGGACT TTTCTGATGA AACAAATACA GAGACTCTCT ACGGCACCTC ACCCCCAAGC 240

ACACCTCGAC AGATGAAACG CCTGTCAGCC AAGCACCAGA GGAACAGCGC AGGGAGGCCG 300

GCCAGCCGAT CGAACTTGAA AGAAAAAATG AACACACCGA GTCAGTCTCC ACATAAAGAT 360

TTGGGGAAGG GAGTGGAGAC CGTGGAAGAA TACAGCTACA AGCAGGAGAA GAAGATTCGA 420

GCAACTCTGA GAACAACGGA GCGAGACCAT AAGAAAAATG CACAGTGCTC GTTCATGTTG 480

GACTCGGTGG CTGGGTCTTT GCCAAAAAAA TCGATTCCAG ATGTGGATCT CAATAAGCCT 540

TACCTCAGTC TCGGCTGTAG CAATGCCAAG CTGCCCGTCT CGATGCCCAT GCCGATAGCC 600

AGAACTGCAC GGCAGACTTC CCGGACTGAC TGCCCCGCAG ATCGCTTAAA GTTCTTTGAA 660

ACACTGCGCC TTTTGCTAAA GCTTACCTCA GTCTCGAAGA AGAAGGACAG GGAGCAGAGG 720

GGACAAGAAA ACACGGCTGC TTTCTGGTTC AACCGATCGA ACGAACTGAT CTGGTTAGAA 780

CTGCAGGCCT GGCACGCGGG CCGCACCATC AATGACCAGG ACCTCTTTCT CTACACAGCC 840

CGCCAGGCCA TCCCAGACAT CATCAATGAG ATCCTCACCT TCAAAGTTAA CTACGGGAGC 900

ATTGCCTTCT CCAGCAATGG AGCCGGTTTC AACGGGCCCT TGGTAGAAGG CCAGTGCAGA 960

ACCCCTCAGG AGACAAACCG TGTGGGCTGC TCATCGTACC ACGAGCACCT CCAGCGCCAG 1020

AGGGTCTCGT TTGAGCAGGT GAAGCGGATA ATGGAGCTGC TGGAGTACAT GGAGGCACTT 1080

TACCCATCCT TGCAGGCTCT GCAGAAGGAC TATGAACGGT ACGCCGCCAA GGACTTTGAG 1140

GACAGAGTGC AGGCGCTCTG CCTGTGGCTC AACATCACGA AAGATCTAAA TCAGAAGCTG 1200

CGGATCATGG GCACCGTGCT GGGCATCAAG AACCTATCAG ACATTGGCTG GCCAGTGTTT 1260

GAAATCCCCT CCCCTCGGCC GTCCAAGGGC TACGAGCCAG AGGACGAGGT CGAGGACACG 1320

GAGGTTGAGC TGAGGGAGCT GGAGAGCGGG ACGGAGGAGA GTGACGAGGA GCCAACCCCC 1380

AGTCCGAGGG TGCCAGAGCT CAGGCTGTCC ACAGACGCCA TCTTGGACAG TCGCTCCCAG 1440

GGCTGCGTCT CCAGGAAGCT GGAGAGGCTC GAGTCAGAGG AAGATTCCAT AGGCTGGGGG 1500

ACAGCGGACT GTGGCCCTGA AGCCAGCAGG CATTGTTTGA CTTCTATCTA TAGACCATTC 1560

GTGGACAAAG CACTGAAGCA AATGGGGCTA AGAAAGTTAA TTTTACGACT TCATAAGCTT 1620

ATGAATGGGT CCTTGCAAAG AGCTCGTGTA GCTCTGGTGA AGGACGACCG TCCAGTGGAG 1680

TTCTCTGACT TTCCAGGTCC CATGTGGGGC TCGGATTATG TGCAGTTGTC GGGAACACCT 1740

CCTTCCTCAG AGCAGAAGTG TAGCGCTGTG TCCTGGGAAG AACTGAGAGC CATGGACCTG 1800

CCTTCCTTTG AGCCCGCCTT CCTGGTGCTC TGTCGGGTCC TGCTGAACGT GATCCACGAG 1860

TGCCTGAAGC TGCGGCTGGA ACAGAGGCCT GCCGGGGAGC CTTCCCTCTT GAGTATCAAA 1920

CAGCTAGTGC GAGAGTGTAA AGAGGTCCTA AAGGGCGGGC TCCTGATGAA GCAGTATTAC 1980

CAGTTCATGC TGCAGGAGGT CCTGGGCGGA CTGGAGAAGA CCGACTGCAA CATGGATGCC 2040

TTTGAGGAGG ACCTGCAGAA GATGCTGATG GTGTATTTTG ATTACATGAG AAGCTGGATC 2100

CAAATGCTAC AGCAGTTACC TCAGGCTTCC CATAGCTTAA AAAACCTGCT AGAAGAGGAA 2160

TGGAATTTCA CCAAAGAAAT AACCCATTAT ATCCGTGGCG GAGAAGCGCA GGCTGGAAAG 2220

CTTTTCTGTG ACATCGCAGG GATGCTGCTG AAATCCACAG GGAGCTTTCT GGAATCCGGC 2280

CTGCAGGAGA GCTGTGCTGA GCTGTGGACC AGCGCCGACG ACAACGGTGC TGCCGACGAG 2340

CTAAGGAGAT CTGTCATCGA GATCAGCCGA GCACTCAAGG AGCTCTTCCA CGAAGCCAGG 2400

GAAAGAGCCT CCAAGGCCCT GGGCTTTGCT AAAATGCTGA GGAAGGACCT AGAAATAGCA 2460

GCAGAGTTCG TGCTATCTGC ATCAGCCCGA GAGCTCCTGG ACGCTCTGAA AGCAAAGCAG 2520

TATGTTAAGG TACAGATTCC CGGGTTAGAG AATTTGCACG TGTTTGTCCC CGACAGCCTC 2580

GCTGAGGAGA AGAAAATTAT TTTGCAGCTA CTCAATGCTG CCACAGGAAA GGACTGCTCA 2640

AAGGATCCAG ACGACGTCTT CATGGATGCC TTCCTGCTCC TGACCAAGCA TGGGGACCGA 2700

GCCCGTGACT CAGAAGATGG CTGGGGCACA TGGGAAGCTC GGGCTGTCAA AATTGTGCCT 2760

CAGGTGGAGA CTGTGGACAC CCTGAGAAGC ATGCAGGTGG ACAACCTTCT GCTGGTTGTC 2820

ATGGAGTCTG CTCACCTCGT ACTTCAGAGA AAAGCCTTCC AGCAGTCCAT TGAGGGGCTG 2880

ATGACTGTAC GCCATGAGCA GACATCTAGC CAGCCCATCA TCGCCAAAGG TTTGCAGCAG 2940

CTCAAGAACG ATGCACTTGA GCTATGCAAC AGAATCAGCG ATGCCATCGA CCGTGTGGAC 3000

CACATGTTCA CCCTGGAGTT CGATGCTGAG GTCGAGGAGT CTGAGTCGGC CACGCTGCAG 3060

CAGTACTACC GAGAAGCCAT GATTCAGGGC TACAACTTTG GGTTTGAGTA TCATAAAGAA 3120

GTTGTTCGTT TGATGTCTGG GGAATTCAGG CAGAAGATAG GAGACAAATA TATAAGCTTC 3180

GCCCAGAAGT GGATGAATTA CGTGCTGACC AAATGCGAGA GCGGCAGAGG CACAAGACCC 3240

AGATGGGCCA CCCAAGGATT TGATTTCCTA CAAGCCATTG AACCTGCCTT TATTTCAGCT 3300

TTACCAGAAG ATGACTTCTT GAGTTTGCAA GCCCTGATGA ATGAGTGCAT CGGGCACGTC 3360

ATAGGAAAGC CACACAGCCC TGTCACAGCT ATCCATCGGA ACAGCCCCCG CCCTGTGAAG 3420

GTGCCCCGAT GCCACAGTGA CCCTCCTAAC CCTCACCTCA TCATCCCGAC TCCAGAGGGA 3480

TTCAGCACCC GGAGCGTGCC TTCCGACGCT CGGACCCATG GCAACTCTGT TGCTGCTGCT 3540

GCTGCTGTTG CTGCCGCCGC CACCACTGCT GCTGGCCGCC CTGGCCCAGG TGGTGGTGAC 3600

TCTGTGCCAG CCAAACCTGT CAACACTGCC CCTGATACCA GGGGTTCCAG TGTCCCTGAA 3660

AACGACCGCT TGGCCTCCAT AGCTGCAGAA CTGCAGTTCA GGTCTCTGAG TCGGCACTCA 3720

AGCCCCACGG AAGAGCGAGA CGAGCCAGCG TATCCTCGGA GTGACTCAAG TGGATCAACT 3780

CGGAGAAGCT GGGAACTTCG AACACTCATC AGCCAGACCA AAGACTCGGC CTCTAAGCAG 3840

GGGCCCATAG AAGCTATCCA GAAGTCAGTC CGACTGTTTG AAGAGAGGAG GTATCGAGAG 3900

ATGAGGAGAA AGAATATCAT CGGCCAAGTG TGCGATACCC CTAAGTCCTA TGATAACGTC 3960

ATGCATGTTG GACTGAGGAA GGTGACATTT AAGTGGCAAA GAGGAAACAA AATTGGAGAA 4020

GGACAGTATG GAAAAGTATA CACCTGCATC AGTGTTGACA CAGGGGAGCT GATGGCCATG 4080

AAGGAGATTC GATTTCAGCC TAACGACCAC AAGACTATCA AGGAGACTGC AGACGAGTTG 4140

AAAATATTTG AAGGCATCAA GCACCCCAAC CTGGTCCGGT ATTTTGGCGT GGAGCTTCAC 4200

AGGGAAGAGA TGTACATCTT CATGGAGTAC TGTGATGAGG GTACACTAGA GGAGGTGTCA 4260

CGACTGGGCC TGCAGGAGCA CGTCATCAGG TTATATACCA AGCAGATCAC TGTCGCCATC 4320

AACGTCCTCC ATGAGCACGG CATCGTTCAC CGAGACATCA AAGGTGCCAA TATCTTCCTT 4380

ACGTCATCTG GACTAATCAA GCTGGGAGAT TTTGGATGCT CTGTAAAACT TAAAAACAAC 4440

GCCCAGACCA TGCCCGGAGA GGTGAACAGC ACCCTAGGGA CAGCAGCTTA CATGGCCCCT 4500

GAAGTTATTA CCCGAGCCAA AGGAGAAGGC CACGGACGTG CGGCAGATAT CTGGAGTCTG 4560

GGGTGCGTCG TCATAGAGAT GGTGACTGGC AAGCGGCCTT GGCATGAGTA TGAACACAAC 4620

TTTCAGATTA TGTACAAGGT GGGGATGGGA CACAAGCCAC CAATCCCGGA AAGGCTAAGC 4680

CCTGAAGGAA AGGCCTTTCT CTCGCACTGC CTGGAAAGTG ACCCGAAGAT ACGGTGGACA 4740

GCCAGCCAGC TCCTCGACCA CGCTTTTGTC AAGGTTTGCA CAGATGAAGA GTGA 4794

Domain Profile

S: 1     wqkrevigkGsfgsvyaaldvdtgellavKeveikpekksekkevkalkeEikllkelkH  60

         wq+++ ig+G++g+vy++++vdtgel+a+Ke++++p++++   ++k+ ++E+k+++ +kH

Q: 1332  WQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHK---TIKETADELKIFEGIKH  1388

         99********************************999876...799**************

S: 61    enivkyyGveveeeelkiflEyvegGslssllekygkleesvikkytkqileglayLHek  120

         +n+v+y+Gve ++ee++if+Ey+++G+l+++ +    l+e+vi+ ytkqi  ++++LHe+

Q: 1389  PNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRL--GLQEHVIRLYTKQITVAINVLHEH  1446

         *******************************884..7***********************

S: 121   kivHRDiKgaNilldssgvvklsDFGaskrlaelntka.aelesltGtlaymaPEvikk.  178

         +ivHRDiKgaNi+l+ssg++kl+DFG+s +l+++++++ +e++s+ Gt+aymaPEvi++

Q: 1447  GIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMpGEVNSTLGTAAYMAPEVITRa  1506

         *******************************9988776256*****************98

S: 179   kekgygrkaDiWslgcvviemltgkrPfaeldeelaimfkvgakktkPeipeklseegkd  238

         k +g+gr+aDiWslgcvviem+tgkrP++e++++++im+kvg+ + kP+ipe+ls+egk+

Q: 1507  KGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGH-KPPIPERLSPEGKA  1565

         899******************************************.**************

S: 239   flekcleldpkkrpsaeeLLkhefl  263

         fl++cle+dpk r++a++LL+h f+

Q: 1566  FLSHCLESDPKIRWTASQLLDHAFV  1590

         **********************996

Domain Sequence
(FASTA)

WQRGNKIGEG QYGKVYTCIS VDTGELMAMK EIRFQPNDHK TIKETADELK IFEGIKHPNL 60

VRYFGVELHR EEMYIFMEYC DEGTLEEVSR LGLQEHVIRL YTKQITVAIN VLHEHGIVHR 120

DIKGANIFLT SSGLIKLGDF GCSVKLKNNA QTMPGEVNST LGTAAYMAPE VITRAKGEGH 180

GRAADIWSLG CVVIEMVTGK RPWHEYEHNF QIMYKVGMGH KPPIPERLSP EGKAFLSHCL 240

ESDPKIRWTA SQLLDHAFV 259

Keyword

Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0048471	; C:perinuclear region of cytoplasm
GO:0005524	; F:ATP binding
GO:0004709	; F:MAP kinase kinase kinase activity
GO:0046872	; F:metal ion binding
GO:0004672	; F:protein kinase activity
GO:0000186	; P:activation of MAPKK activity
GO:0060718	; P:chorionic trophoblast cell differentiation
GO:0048263	; P:determination of dorsal identity
GO:0019100	; P:male germ-line sex determination
GO:0000165	; P:MAPK cascade
GO:0001890	; P:placenta development
GO:0043507	; P:positive regulation of JUN kinase activity
GO:0010468	; P:regulation of gene expression

KEGG

mmu:26407

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013